Restriction map for
pFN21AF0007
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# NO CUT ENZYMES ( 100 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BcgI BciVI BfrI BglII BlnI
BsaBI BsaI BseAI BsiEI BsiWI
BspDI BspEI BspHI BspLU11I BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoT22I FseI FspI HincII
HindII HpaI KasI KpnI MamI
MfeI MluI MroI MslI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
SexAI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
TfiI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 805 CGCG CG^CG
AcsI 1771 GAATTT [GA]^AATT[CT]
AcyI 306 GGCGTC G[GA]^CG[CT]C
AocI 1702 CCTGAGG CC^T[ACGT]AGG
ApoI 1771 GAATTT [GA]^AATT[CT]
AvaI 1730 CCCGGG C^[CT]CG[GA]G
BamHI 2043 GGATCC G^GATCC
BbrPI 1420 CACGTG CAC^GTG
BbsI 1792 GTCTTC GAAGAC(2/6)
BclI 918 TGATCA T^GATCA
BfaI 328 CTAG C^TAG
BpuAI 1792 GTCTTC GAAGAC(2/6)
BsaAI 1420 CACGTG [CT]AC^GT[GA]
BsaHI 306 GGCGTC G[GA]^CG[CT]C
BsmBI 1351 GAGACG CGTCTC(1/5)
BsmI 1642 GCATTC GAATGC(1/-1)
BsoBI 1730 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1661 TGTACA T^GTACA
BsrDI 623 GCAATG GCAATG(2/0)
BsrGI 1661 TGTACA T^GTACA
BssSI 1395 CACGAG CACGAG(-5/-1)
BstUI 805 CGCG CG^CG
BstXI 1026 CCAGAACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1702 CCTGAGG CC^T[ACGT]AGG
DrdI 1510 GACGAATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1702 CCTGAGG CC^T[ACGT]AGG
EcoRV 1565 GATATC GAT^ATC
FbaI 918 TGATCA T^GATCA
Hin1I 306 GGCGTC G[GA]^CG[CT]C
HindIII 43 AAGCTT A^AGCTT
KspI 804 CCGCGG CCGC^GG
MaeI 328 CTAG C^TAG
MseI 638 TTAA T^TAA
MvnI 805 CGCG CG^CG
NspI 795 GCATGC [GA]CATG^[CT]
PmaCI 1420 CACGTG CAC^GTG
PmlI 1420 CACGTG CAC^GTG
SacI 1360 GAGCTC GAGCT^C
SacII 804 CCGCGG CCGC^GG
SapI 1813 GAAGAGC GCTCTTC(1/4)
ScaI 596 AGTACT AGT^ACT
SfiI 1392 GGCCACGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1730 CCCGGG CCC^GGG
SphI 795 GCATGC GCATG^C
SspBI 1661 TGTACA T^GTACA
Tru9I 638 TTAA T^TAA
Tsp509I 1772 AATT ^AATT
XmaCI 1730 CCCGGG C^CCGGG
XmaI 1730 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
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AflIII 465 ACGTGT A^C[GA][CT]GT
1419 ACACGT
Aor51HI 989 AGCGCT AGC^GCT
1211 AGCGCT
BalI 1391 TGGCCA TGG^CCA
2049 TGGCCA
BsaWI 209 ACCGGA [AT]^CCGG[AT]
1672 ACCGGA
BsmAI 1351 GAGAC GTCTC(1/5)
554 GTCTC
BsrFI 27 GCCGGC [GA]^CCGG[CT]
2028 GCCGGC
Cfr10I 27 GCCGGC [GA]^CCGG[CT]
2028 GCCGGC
EaeI 1391 TGGCCA [CT]^GGCC[GA]
2049 TGGCCA
EarI 195 GAAGAG CTCTTC(1/4)
1813 GAAGAG
Eco47III 989 AGCGCT AGC^GCT
1211 AGCGCT
EcoNI 1182 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1647 CCTGGCACAGG
HgaI 307 GCGTC GACGC(5/10)
2010 GCGTC
HinfI 299 GACTC G^A[ACGT]TC
1997 GACTC
Ksp632I 195 GAAGAG CTCTTC(1/4)
1813 GAAGAG
MaeII 465 ACGT A^CGT
1421 ACGT
MluNI 1391 TGGCCA TGG^CCA
2049 TGGCCA
MscI 1391 TGGCCA TGG^CCA
2049 TGGCCA
NaeI 27 GCCGGC GCC^GGC
2028 GCCGGC
NcoI 528 CCATGG C^CATGG
1032 CCATGG
NgoMIV 27 GCCGGC G^CCGGC
2028 GCCGGC
PflMI 171 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1383 CCAAGCCCTGG
PleI 299 GACTC GAGTC(4/5)
1997 GACTC
PpuMI 737 GGGACCC [GA]G^G[AT]CC[CT]
1295 GGGACCT
PstI 412 CTGCAG CTGCA^G
1186 CTGCAG
PvuII 61 CAGCTG CAG^CTG
607 CAGCTG
SmlI 700 CTCAAG C^T[CT][GA]AG
732 CTTGAG
TaqI 665 TCGA T^CGA
1995 TCGA
TthHB8I 665 TCGA T^CGA
1995 TCGA
Van91I 171 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1383 CCAAGCCCTGG
XcmI 171 CCAGAAACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1637 CCACAGCATTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaII BanI BanII
BbvCI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspMI BsrBI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI XhoII