(Circular) (Six-base) MAPSORT of: pFN21AF0007.seq Check: 5700 from: 1 to: 6775 pFN21AF0007 6775 bp With 182 enzymes: SgfI * October 20, 2009 10:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2356 3913 2356 Size: 1557 5218 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4585 278 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4118 1937 Size: 2181 4594 Acc65I G'GTAC_C Cuts at: 4096 4096 Size: 6775 AclI AA'CG_TT Cuts at: 1563 4902 5275 1563 Size: 3339 373 3063 Fragments arranged by size: 3339 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2063 2219 2849 2873 3069 3245 4838 6222 Size: 431 156 630 24 196 176 1593 1384 Cuts at: 6222 6490 1632 Size: 268 1917 Fragments arranged by size: 1917 1593 1384 630 431 268 196 176 156 24 AfeI AGC'GCT Cuts at: 2996 3218 2996 Size: 222 6553 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6578 AflIII A'CryG_T Cuts at: 1203 2470 3424 5674 6651 1203 Size: 1267 954 2250 977 1327 Fragments arranged by size: 2250 1327 1267 977 954 AgeI A'CCGG_T Cuts at: 5606 5606 Size: 6775 AhdI GACnn_n'nnGTC Cuts at: 5504 5504 Size: 6775 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6775 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6775 AlwNI CAG_nnn'CTG Cuts at: 2182 2569 2614 2921 3664 3871 4518 6090 2182 Size: 387 45 307 743 207 647 1572 2867 Fragments arranged by size: 2867 1572 743 647 387 307 207 45 ApaI G_GGCC'C Cuts at: 3409 3742 3933 3409 Size: 333 191 6251 Fragments arranged by size: 6251 333 191 ApaLI G'TGCA_C Cuts at: 4832 5988 4832 Size: 1156 5619 ApoI r'AATT_y Cuts at: 1493 3776 4084 4310 4462 6571 1493 Size: 2283 308 226 152 2109 1697 Fragments arranged by size: 2283 2109 1697 308 226 152 AseI AT'TA_AT Cuts at: 160 1032 5329 160 Size: 872 4297 1606 Fragments arranged by size: 4297 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 AvaI C'yCGr_G Cuts at: 1870 1943 3735 4100 1870 Size: 73 1792 365 4545 Fragments arranged by size: 4545 1792 365 73 BamHI G'GATC_C Cuts at: 4048 4105 4472 4048 Size: 57 367 6351 Fragments arranged by size: 6351 367 57 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2211 2255 3994 4096 5551 Size: 325 217 627 424 44 1739 102 1455 Cuts at: 5551 618 Size: 1842 Fragments arranged by size: 1842 1739 1455 627 424 325 217 102 44 BanII G_rGCy'C Cuts at: 1391 3369 3409 3642 3742 3933 4094 1391 Size: 1978 40 233 100 191 161 4072 Fragments arranged by size: 4072 1978 233 191 161 100 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3790 928 Size: 971 1891 3913 Fragments arranged by size: 3913 1891 971 BbvCI CC'TCA_GC Cuts at: 2409 2898 3318 2409 Size: 489 420 5866 Fragments arranged by size: 5866 489 420 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4966 5000 1903 Size: 34 50 34 2945 34 3678 Fragments arranged by size: 3678 2945 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4667 5877 4667 Size: 1210 5565 BclI T'GATC_A Cuts at: 1548 2923 1548 Size: 1375 5400 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2285 3182 3404 Size: 107 122 71 631 605 613 897 222 Cuts at: 3404 5386 136 Size: 1982 1525 Fragments arranged by size: 1982 1525 897 631 613 605 222 122 107 71 BglII A'GATC_T Cuts at: 6770 6770 Size: 6775 BlpI GC'TnA_GC Cuts at: 2069 2435 3327 3698 4192 2069 Size: 366 892 371 494 4652 Fragments arranged by size: 4652 892 494 371 366 Bme1580I G_kGCm'C Cuts at: 1790 2214 3151 3409 3718 3742 3933 4836 5992 Size: 424 937 258 309 24 191 903 1156 Cuts at: 5992 1790 Size: 2573 Fragments arranged by size: 2573 1156 937 903 424 309 258 191 24 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6775 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2584 2593 4506 Size: 416 53 83 399 24 1167 9 1913 Cuts at: 4506 5464 442 Size: 958 1753 Fragments arranged by size: 1913 1753 1167 958 416 399 83 53 24 9 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6775 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2159 2291 2378 2546 2816 2960 2999 Size: 893 27 132 87 168 270 144 39 Cuts at: 2999 3083 3509 3787 5435 1239 Size: 84 426 278 1648 2579 Fragments arranged by size: 2579 1648 893 426 278 270 168 144 132 87 84 39 27 Bpu10I CC'TnA_GC Cuts at: 2372 2409 2759 2898 3318 3564 2372 Size: 37 350 139 420 246 5583 Fragments arranged by size: 5583 420 350 246 139 37 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2690 2758 4900 5765 6063 2690 Size: 68 2142 865 298 3402 Fragments arranged by size: 3402 2142 865 298 68 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5438 882 Size: 617 3939 2219 Fragments arranged by size: 3939 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3427 4566 493 Size: 711 2223 1139 2702 Fragments arranged by size: 2702 2223 1139 711 BsaBI GATnn'nnATC Cuts at: 6769 6769 Size: 6775 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2312 4582 4964 5599 Size: 53 83 186 936 779 2270 382 635 Cuts at: 5599 275 Size: 1451 Fragments arranged by size: 2270 1451 936 779 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2214 3677 5208 5606 5880 6027 6438 2214 Size: 1463 1531 398 274 147 411 2551 Fragments arranged by size: 2551 1531 1463 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3467 3497 1178 Size: 30 165 30 2064 30 4456 Fragments arranged by size: 4456 2064 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3368 3389 3515 3707 3876 1186 Size: 2182 21 126 192 169 4085 Fragments arranged by size: 4085 2182 192 169 126 21 BseYI C'CCAG_C Cuts at: 2288 2607 2838 3890 5978 6474 2288 Size: 319 231 1052 2088 496 2589 Fragments arranged by size: 2589 2088 1052 496 319 231 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3405 3708 3891 3921 1163 Size: 573 1669 303 183 30 4017 Fragments arranged by size: 4017 1669 573 303 183 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4225 4986 6014 1540 Size: 275 186 2224 761 1028 2301 Fragments arranged by size: 2301 2224 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2576 2736 3369 4836 4921 5992 2576 Size: 160 633 1467 85 1071 3359 Fragments arranged by size: 3359 1467 1071 633 160 85 BsmI GAATG_Cn' Cuts at: 3647 4286 4379 3647 Size: 639 93 6043 Fragments arranged by size: 6043 639 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3350 3350 Size: 6775 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2214 2576 2736 3151 3369 3409 3642 Size: 399 424 362 160 415 218 40 233 Cuts at: 3642 3718 3742 3933 4094 4836 4921 5992 1391 Size: 76 24 191 161 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 424 415 399 362 233 218 191 161 160 85 76 40 24 BspEI T'CCGG_A Cuts at: 6438 6438 Size: 6775 BspHI T'CATG_A Cuts at: 4664 6394 4664 Size: 1730 5045 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2356 2646 3913 4130 844 Size: 1512 290 1267 217 3489 Fragments arranged by size: 3489 1512 1267 290 217 BsrBI CCG'CTC Cuts at: 1394 1823 2220 3091 3268 4662 1394 Size: 429 397 871 177 1394 3507 Fragments arranged by size: 3507 1394 871 429 397 177 BsrDI GCAATG_nn' Cuts at: 65 1248 2635 5270 5444 65 Size: 1183 1387 2635 174 1396 Fragments arranged by size: 2635 1396 1387 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2032 4033 5419 5606 1700 Size: 110 222 2001 1386 187 2869 Fragments arranged by size: 2869 2001 1386 222 187 110 BsrGI T'GTAC_A Cuts at: 96 3666 96 Size: 3570 3205 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6775 BssSI C'ACGA_G Cuts at: 3400 4835 5847 3400 Size: 1435 1012 4328 Fragments arranged by size: 4328 1435 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6775 BstBI TT'CG_AA Cuts at: 5579 5579 Size: 6775 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6775 BstXI CCAn_nnnn'nTGG Cuts at: 3038 3038 Size: 6775 BstYI r'GATC_y Cuts at: 1972 2104 2123 2538 3027 4048 4063 4105 4472 Size: 132 19 415 489 1021 15 42 367 Cuts at: 4472 4857 4874 6326 6412 6424 6770 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1021 489 415 385 367 346 132 86 42 19 17 15 12 Bsu36I CC'TnA_GG Cuts at: 3708 3708 Size: 6775 BtgI C'CryG_G Cuts at: 513 1065 2283 2533 2809 3037 513 Size: 552 1218 250 276 228 4251 Fragments arranged by size: 4251 1218 552 276 250 228 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6036 Fragments arranged by size: 6036 683 56 BtsI GCAGTG_nn' Cuts at: 4291 5085 5105 4291 Size: 794 20 5961 Fragments arranged by size: 5961 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6775 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6740 DraI TTT'AAA Cuts at: 4079 4079 Size: 6775 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3521 5782 809 Size: 507 2205 2261 1802 Fragments arranged by size: 2261 2205 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3396 4054 4222 Size: 54 1425 50 275 18 1566 658 168 Cuts at: 4222 5111 6740 8 Size: 889 1629 43 Fragments arranged by size: 1629 1566 1425 889 658 275 168 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4222 1537 Size: 275 2410 4090 Fragments arranged by size: 4090 2410 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2195 3813 4705 1353 Size: 842 1618 892 3423 Fragments arranged by size: 3423 1618 892 842 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2117 2365 2575 3131 5362 5736 Size: 1064 138 528 248 210 556 2231 374 Cuts at: 5736 5882 387 Size: 146 1280 Fragments arranged by size: 2231 1280 1064 556 528 374 248 210 146 138 EcoICRI GAG'CTC Cuts at: 3367 3367 Size: 6775 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2132 2159 2219 2291 2378 2546 Size: 393 431 69 27 60 72 87 168 Cuts at: 2546 2816 2849 2873 2960 2999 3069 3083 3245 Size: 270 33 24 87 39 70 14 162 Cuts at: 3245 3509 3787 4838 5435 6222 6490 1239 Size: 264 278 1051 597 787 268 1524 Fragments arranged by size: 1524 1051 787 597 431 393 278 270 268 264 168 162 87 87 72 70 69 60 39 33 27 24 14 EcoNI CCTnn'n_nnAGG Cuts at: 3191 3656 3191 Size: 465 6310 EcoO109I rG'GnC_Cy Cuts at: 2710 2743 3301 3405 3406 3661 3930 2710 Size: 33 558 104 1 255 269 5555 Fragments arranged by size: 5555 558 269 255 104 33 1 EcoRI G'AATT_C Cuts at: 4084 4462 4084 Size: 378 6397 EcoRV GAT'ATC Cuts at: 3572 3572 Size: 6775 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2071 3842 2071 Size: 1771 5004 FspI TGC'GCA Cuts at: 5281 5281 Size: 6775 HaeII r_GCGC'y Cuts at: 1678 2266 2358 2998 3220 5922 1678 Size: 588 92 640 222 2702 2531 Fragments arranged by size: 2702 2531 640 588 222 92 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2140 2428 2460 3123 Size: 32 135 32 536 32 288 32 663 Cuts at: 3123 3155 3982 4014 5416 5448 5490 5522 1373 Size: 32 827 32 1402 32 42 32 2626 Fragments arranged by size: 2626 1402 827 663 536 288 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4119 4365 669 Size: 988 281 2181 246 3079 Fragments arranged by size: 3079 2181 988 281 246 HindIII A'AGCT_T Cuts at: 748 2048 748 Size: 1300 5475 HpaI GTT'AAC Cuts at: 4365 4365 Size: 6775 KpnI G_GTAC'C Cuts at: 4100 4100 Size: 6775 MfeI C'AATT_G Cuts at: 4374 4374 Size: 6775 MluI A'CGCG_T Cuts at: 6651 6651 Size: 6775 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2426 3335 3903 5889 6073 1305 Size: 1121 909 568 1986 184 2007 Fragments arranged by size: 2007 1986 1121 909 568 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3398 4056 10 Size: 54 1425 343 1566 658 2729 Fragments arranged by size: 2729 1566 1425 658 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4733 5092 5251 518 Size: 648 3567 359 159 2042 Fragments arranged by size: 3567 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2068 2464 2614 2811 3317 4191 4868 6016 Size: 462 396 150 197 506 874 677 1148 Cuts at: 6016 6261 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 874 677 506 462 396 245 197 150 NaeI GCC'GGC Cuts at: 2034 4035 2034 Size: 2001 4774 NcoI C'CATG_G Cuts at: 513 1065 2533 3037 513 Size: 552 1468 504 4251 Fragments arranged by size: 4251 1468 552 504 NdeI CA'TA_TG Cuts at: 387 387 Size: 6775 NgoMIV G'CCGG_C Cuts at: 2032 4033 2032 Size: 2001 4774 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6775 NotI GC'GGCC_GC Cuts at: 4222 4222 Size: 6775 NspI r_CATG'y Cuts at: 1132 1229 2804 4133 5678 1132 Size: 97 1575 1329 1545 2229 Fragments arranged by size: 2229 1575 1545 1329 97 PasI CC'CwG_GG Cuts at: 1348 3836 3974 1348 Size: 2488 138 4149 Fragments arranged by size: 4149 2488 138 PciI A'CATG_T Cuts at: 5674 5674 Size: 6775 PflMI CCAn_nnn'nTGG Cuts at: 1108 2182 3394 1108 Size: 1074 1212 4489 Fragments arranged by size: 4489 1212 1074 PfoI T'CCnGG_A Cuts at: 2058 2427 2793 2937 3582 3981 2058 Size: 369 366 144 645 399 4852 Fragments arranged by size: 4852 645 399 369 366 144 PmeI GTTT'AAAC Cuts at: 4079 4079 Size: 6775 PmlI CAC'GTG Cuts at: 3427 3427 Size: 6775 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4844 4876 6357 6389 4844 Size: 32 1481 32 5230 Fragments arranged by size: 5230 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2743 3301 2743 Size: 558 6217 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6775 PsiI TTA'TAA Cuts at: 4345 4345 Size: 6775 PspOMI G'GGCC_C Cuts at: 3405 3738 3929 3405 Size: 333 191 6251 Fragments arranged by size: 6251 333 191 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3102 3134 3102 Size: 32 6743 PstI C_TGCA'G Cuts at: 830 2421 3195 4127 830 Size: 1591 774 932 3478 Fragments arranged by size: 3478 1591 932 774 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6775 PvuII CAG'CTG Cuts at: 2068 2614 2068 Size: 546 6229 SacI G_AGCT'C Cuts at: 3369 3369 Size: 6775 SacII CC_GC'GG Cuts at: 2812 2812 Size: 6775 SalI G'TCGA_C Cuts at: 1936 4117 1936 Size: 2181 4594 SanDI GG'GwC_CC Cuts at: 2743 2743 Size: 6775 SapI GCTCTTCn'nnn_ Cuts at: 3813 3813 Size: 6775 SbfI CC_TGCA'GG Cuts at: 4127 4127 Size: 6775 ScaI AGT'ACT Cuts at: 2603 5023 6735 2603 Size: 2420 1712 2643 Fragments arranged by size: 2643 2420 1712 SfcI C'TryA_G Cuts at: 826 1045 2128 2417 3191 4123 5258 5939 6130 Size: 219 1083 289 774 932 1135 681 191 Cuts at: 6130 826 Size: 1471 Fragments arranged by size: 1471 1135 1083 932 774 681 289 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3404 3404 Size: 6775 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6775 SmaI CCC'GGG Cuts at: 1872 3737 4102 1872 Size: 1865 365 4545 Fragments arranged by size: 4545 1865 365 SmlI C'TyrA_G Cuts at: 820 1017 1943 2705 2737 4879 5780 6042 820 Size: 197 926 762 32 2142 901 262 1553 Fragments arranged by size: 2142 1553 926 901 762 262 197 32 SnaBI TAC'GTA Cuts at: 493 4566 493 Size: 4073 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6775 SphI G_CATG'C Cuts at: 1132 2804 4133 1132 Size: 1672 1329 3774 Fragments arranged by size: 3774 1672 1329 SspI AAT'ATT Cuts at: 5 52 4699 5 Size: 47 4647 2081 Fragments arranged by size: 4647 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6775 StyI C'CwwG_G Cuts at: 513 1065 2358 2481 2533 3037 4214 513 Size: 552 1293 123 52 504 1177 3074 Fragments arranged by size: 3074 1293 1177 552 504 123 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2334 4074 4802 4819 4972 5157 5602 Size: 1255 754 1740 728 17 153 185 445 Cuts at: 5602 325 Size: 1498 Fragments arranged by size: 1740 1498 1255 754 728 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2601 3666 5021 Size: 274 80 33 51 1443 624 1065 1355 Cuts at: 5021 6733 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1355 1065 624 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2459 2491 3467 3499 6735 6767 2459 Size: 32 976 32 3236 32 2467 Fragments arranged by size: 3236 2467 976 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6775 XbaI T'CTAG_A Cuts at: 4111 4111 Size: 6775 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2183 3649 2183 Size: 1466 5309 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6775 XmaI C'CCGG_G Cuts at: 1870 3735 4100 1870 Size: 1865 365 4545 Fragments arranged by size: 4545 1865 365 XmnI GAAnn'nnTTC Cuts at: 4904 4904 Size: 6775 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4583 276 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII FseI FspAI KasI NarI NruI NsiI PacI PspXI RsrII SexAI SfoI SrfI SwaI