Restriction map for
pFN21AF0007
(Linear) (Six-base) MAPSORT of: FLJ00007.seq Check: 4506 from: 1 to: 2070
FLJ00007.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 351 1908 2070
Size: 351 1557 162
Fragments arranged by size:
1557 351 162
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 214 844 868 1064 1240 2070
Size: 58 156 630 24 196 176 830
Fragments arranged by size:
830 630 196 176 156 58 24
AfeI AGC'GCT
Cuts at: 0 991 1213 2070
Size: 991 222 857
Fragments arranged by size:
991 857 222
AflIII A'CryG_T
Cuts at: 0 465 1419 2070
Size: 465 954 651
Fragments arranged by size:
954 651 465
AlwNI CAG_nnn'CTG
Cuts at: 0 177 564 609 916 1659 1866 2070
Size: 177 387 45 307 743 207 204
Fragments arranged by size:
743 387 307 207 204 177 45
ApaI G_GGCC'C
Cuts at: 0 1404 1737 1928 2070
Size: 1404 333 191 142
Fragments arranged by size:
1404 333 191 142
ApoI r'AATT_y
Cuts at: 0 1771 2070
Size: 1771 299
AvaI C'yCGr_G
Cuts at: 0 1730 2070
Size: 1730 340
BamHI G'GATC_C
Cuts at: 0 2043 2070
Size: 2043 27
BanI G'GyrC_C
Cuts at: 0 206 250 1989 2070
Size: 206 44 1739 81
Fragments arranged by size:
1739 206 81 44
BanII G_rGCy'C
Cuts at: 0 1364 1404 1637 1737 1928 2070
Size: 1364 40 233 100 191 142
Fragments arranged by size:
1364 233 191 142 100 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 1785 2070
Size: 1785 285
BbvCI CC'TCA_GC
Cuts at: 0 404 893 1313 2070
Size: 404 489 420 757
Fragments arranged by size:
757 489 420 404
BclI T'GATC_A
Cuts at: 0 918 2070
Size: 918 1152
BglI GCCn_nnn'nGGC
Cuts at: 0 280 1177 1399 2070
Size: 280 897 222 671
Fragments arranged by size:
897 671 280 222
BlpI GC'TnA_GC
Cuts at: 0 64 430 1322 1693 2070
Size: 64 366 892 371 377
Fragments arranged by size:
892 377 371 366 64
Bme1580I G_kGCm'C
Cuts at: 0 209 1146 1404 1713 1737 1928 2070
Size: 209 937 258 309 24 191 142
Fragments arranged by size:
937 309 258 209 191 142 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 579 588 2070
Size: 579 9 1482
Fragments arranged by size:
1482 579 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 154 286 373 541 811 955 994
Size: 127 27 132 87 168 270 144 39
Cuts at: 994 1078 1504 1782 2070
Size: 84 426 278 288
Fragments arranged by size:
426 288 278 270 168 144 132 127
87 84 39 27
Bpu10I CC'TnA_GC
Cuts at: 0 367 404 754 893 1313 1559 2070
Size: 367 37 350 139 420 246 511
Fragments arranged by size:
511 420 367 350 246 139 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 685 753 2070
Size: 685 68 1317
Fragments arranged by size:
1317 685 68
BsaAI yAC'GTr
Cuts at: 0 1422 2070
Size: 1422 648
BsaHI Gr'CG_yC
Cuts at: 0 307 2070
Size: 307 1763
BsaWI w'CCGG_w
Cuts at: 0 209 1672 2070
Size: 209 1463 398
Fragments arranged by size:
1463 398 209
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1462 1492 2070
Size: 1462 30 578
Fragments arranged by size:
1462 578 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1363 1384 1510 1702 1871 2070
Size: 1363 21 126 192 169 199
Fragments arranged by size:
1363 199 192 169 126 21
BseYI C'CCAG_C
Cuts at: 0 283 602 833 1885 2070
Size: 283 319 231 1052 185
Fragments arranged by size:
1052 319 283 231 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1400 1703 1886 1916 2070
Size: 1400 303 183 30 154
Fragments arranged by size:
1400 303 183 154 30
BsiHKAI G_wGCw'C
Cuts at: 0 571 731 1364 2070
Size: 571 160 633 706
Fragments arranged by size:
706 633 571 160
BsmI GAATG_Cn'
Cuts at: 0 1642 2070
Size: 1642 428
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1345 2070
Size: 1345 725
Bsp1286I G_dGCh'C
Cuts at: 0 209 571 731 1146 1364 1404 1637 1713
Size: 209 362 160 415 218 40 233 76
Cuts at: 1713 1737 1928 2070
Size: 24 191 142
Fragments arranged by size:
415 362 233 218 209 191 160 142
76 40 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 351 641 1908 2070
Size: 351 290 1267 162
Fragments arranged by size:
1267 351 290 162
BsrBI CCG'CTC
Cuts at: 0 215 1086 1263 2070
Size: 215 871 177 807
Fragments arranged by size:
871 807 215 177
BsrDI GCAATG_nn'
Cuts at: 0 630 2070
Size: 630 1440
BsrFI r'CCGG_y
Cuts at: 0 27 2028 2070
Size: 27 2001 42
Fragments arranged by size:
2001 42 27
BsrGI T'GTAC_A
Cuts at: 0 1661 2070
Size: 1661 409
BssSI C'ACGA_G
Cuts at: 0 1395 2070
Size: 1395 675
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1033 2070
Size: 1033 1037
BstYI r'GATC_y
Cuts at: 0 99 118 533 1022 2043 2058 2070
Size: 99 19 415 489 1021 15 12
Fragments arranged by size:
1021 489 415 99 19 15 12
Bsu36I CC'TnA_GG
Cuts at: 0 1703 2070
Size: 1703 367
BtgI C'CryG_G
Cuts at: 0 278 528 804 1032 2070
Size: 278 250 276 228 1038
Fragments arranged by size:
1038 278 276 250 228
DrdI GACnn_nn'nnGTC
Cuts at: 0 1516 2070
Size: 1516 554
EaeI y'GGCC_r
Cuts at: 0 1391 2049 2070
Size: 1391 658 21
Fragments arranged by size:
1391 658 21
EarI CTCTTCn'nnn_
Cuts at: 0 190 1808 2070
Size: 190 1618 262
Fragments arranged by size:
1618 262 190
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 112 360 570 1126 2070
Size: 112 248 210 556 944
Fragments arranged by size:
944 556 248 210 112
EcoICRI GAG'CTC
Cuts at: 0 1362 2070
Size: 1362 708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 127 154 214 286 373 541 811
Size: 58 69 27 60 72 87 168 270
Cuts at: 811 844 868 955 994 1064 1078 1240 1504
Size: 33 24 87 39 70 14 162 264
Cuts at: 1504 1782 2070
Size: 278 288
Fragments arranged by size:
288 278 270 264 168 162 87 87
72 70 69 60 58 39 33 27
24 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1186 1651 2070
Size: 1186 465 419
Fragments arranged by size:
1186 465 419
EcoO109I rG'GnC_Cy
Cuts at: 0 705 738 1296 1400 1401 1656 1925 2070
Size: 705 33 558 104 1 255 269 145
Fragments arranged by size:
705 558 269 255 145 104 33 1
EcoRV GAT'ATC
Cuts at: 0 1567 2070
Size: 1567 503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 66 1837 2070
Size: 66 1771 233
Fragments arranged by size:
1771 233 66
HaeII r_GCGC'y
Cuts at: 0 261 353 993 1215 2070
Size: 261 92 640 222 855
Fragments arranged by size:
855 640 261 222 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 423 455 1118 1150 1977 2009
Size: 103 32 288 32 663 32 827 32
Cuts at: 2009 2070
Size: 61
Fragments arranged by size:
827 663 288 103 61 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 43 2070
Size: 43 2027
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1330 1898 2070
Size: 421 909 568 172
Fragments arranged by size:
909 568 421 172
MscI TGG'CCA
Cuts at: 0 1393 2051 2070
Size: 1393 658 19
Fragments arranged by size:
1393 658 19
MspA1I CmG'CkG
Cuts at: 0 63 459 609 806 1312 2070
Size: 63 396 150 197 506 758
Fragments arranged by size:
758 506 396 197 150 63
NaeI GCC'GGC
Cuts at: 0 29 2030 2070
Size: 29 2001 40
Fragments arranged by size:
2001 40 29
NcoI C'CATG_G
Cuts at: 0 528 1032 2070
Size: 528 504 1038
Fragments arranged by size:
1038 528 504
NgoMIV G'CCGG_C
Cuts at: 0 27 2028 2070
Size: 27 2001 42
Fragments arranged by size:
2001 42 27
NspI r_CATG'y
Cuts at: 0 799 2070
Size: 799 1271
PasI CC'CwG_GG
Cuts at: 0 1831 1969 2070
Size: 1831 138 101
Fragments arranged by size:
1831 138 101
PflMI CCAn_nnn'nTGG
Cuts at: 0 177 1389 2070
Size: 177 1212 681
Fragments arranged by size:
1212 681 177
PfoI T'CCnGG_A
Cuts at: 0 53 422 788 932 1577 1976 2070
Size: 53 369 366 144 645 399 94
Fragments arranged by size:
645 399 369 366 144 94 53
PmlI CAC'GTG
Cuts at: 0 1422 2070
Size: 1422 648
PpuMI rG'GwC_Cy
Cuts at: 0 738 1296 2070
Size: 738 558 774
Fragments arranged by size:
774 738 558
PspOMI G'GGCC_C
Cuts at: 0 1400 1733 1924 2070
Size: 1400 333 191 146
Fragments arranged by size:
1400 333 191 146
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1097 1129 2070
Size: 1097 32 941
Fragments arranged by size:
1097 941 32
PstI C_TGCA'G
Cuts at: 0 416 1190 2070
Size: 416 774 880
Fragments arranged by size:
880 774 416
PvuII CAG'CTG
Cuts at: 0 63 609 2070
Size: 63 546 1461
Fragments arranged by size:
1461 546 63
SacI G_AGCT'C
Cuts at: 0 1364 2070
Size: 1364 706
SacII CC_GC'GG
Cuts at: 0 807 2070
Size: 807 1263
SanDI GG'GwC_CC
Cuts at: 0 738 2070
Size: 738 1332
SapI GCTCTTCn'nnn_
Cuts at: 0 1808 2070
Size: 1808 262
ScaI AGT'ACT
Cuts at: 0 598 2070
Size: 598 1472
SfcI C'TryA_G
Cuts at: 0 123 412 1186 2070
Size: 123 289 774 884
Fragments arranged by size:
884 774 289 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1399 2070
Size: 1399 671
SmaI CCC'GGG
Cuts at: 0 1732 2070
Size: 1732 338
SmlI C'TyrA_G
Cuts at: 0 700 732 2070
Size: 700 32 1338
Fragments arranged by size:
1338 700 32
SphI G_CATG'C
Cuts at: 0 799 2070
Size: 799 1271
StyI C'CwwG_G
Cuts at: 0 353 476 528 1032 2070
Size: 353 123 52 504 1038
Fragments arranged by size:
1038 504 353 123 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 329 2069 2070
Size: 329 1740 1
Fragments arranged by size:
1740 329 1
TatI w'GTAC_w
Cuts at: 0 596 1661 2070
Size: 596 1065 409
Fragments arranged by size:
1065 596 409
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 454 486 1462 1494 2070
Size: 454 32 976 32 576
Fragments arranged by size:
976 576 454 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 178 1644 2070
Size: 178 1466 426
Fragments arranged by size:
1466 426 178
XmaI C'CCGG_G
Cuts at: 0 1730 2070
Size: 1730 340
Enzymes that do cut:
AarI AcuI AfeI AflIII AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BclI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI
Bsu36I BtgI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HindIII MmeI
MscI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PsrI PstI PvuII SacI
SacII SanDI SapI ScaI SfcI SfiI SmaI SmlI
SphI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BciVI BfrBI BglII BmgBI BmtI BplI
BsaI BsaBI BsiEI BsiWI BspEI BspHI BssHII BstAPI
BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII EagI EcoRI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI MslI NarI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PpiI PshAI
PsiI PspXI PvuI RsrII SalI SbfI SexAI SfoI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmnI ZraI