(Circular) (Six-base) MAPSORT of: pFN21AB6525.seq Check: 1172 from: 1 to: 5800 pFN21AB6525 5800 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3143 1937 Size: 1206 4594 Acc65I G'GTAC_C Cuts at: 3121 3121 Size: 5800 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2645 3863 5247 5515 1632 Size: 1013 1218 1384 268 1917 Fragments arranged by size: 1917 1384 1218 1013 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 4699 5676 1203 Size: 3496 977 1327 Fragments arranged by size: 3496 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 4529 4529 Size: 5800 AleI CACnn'nnGTG Cuts at: 1166 2384 1166 Size: 1218 4582 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5800 AlwNI CAG_nnn'CTG Cuts at: 2179 2350 2945 3543 5115 2179 Size: 171 595 598 1572 2864 Fragments arranged by size: 2864 1572 598 595 171 ApaI G_GGCC'C Cuts at: 2168 2372 2168 Size: 204 5596 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 2428 3109 3335 3487 5596 1493 Size: 935 681 226 152 2109 1697 Fragments arranged by size: 2109 1697 935 681 226 152 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 2128 2468 3125 1870 Size: 73 185 340 657 4545 Fragments arranged by size: 4545 657 340 185 73 BamHI G'GATC_C Cuts at: 2768 3130 3497 2768 Size: 362 367 5071 Fragments arranged by size: 5071 367 362 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2386 2447 2732 3121 4576 Size: 325 217 627 599 61 285 389 1455 Cuts at: 4576 618 Size: 1842 Fragments arranged by size: 1842 1455 627 599 389 325 285 217 61 BanII G_rGCy'C Cuts at: 1391 2043 2168 2214 2372 2529 2610 2959 3119 Size: 652 125 46 158 157 81 349 160 Cuts at: 3119 1391 Size: 4072 Fragments arranged by size: 4072 652 349 160 158 157 125 81 46 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4829 BbvCI CC'TCA_GC Cuts at: 2022 2022 Size: 5800 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3991 4025 1903 Size: 34 2054 34 3678 Fragments arranged by size: 3678 2054 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 1548 Size: 5800 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2199 2355 4411 Size: 107 122 71 631 605 527 156 2056 Cuts at: 4411 136 Size: 1525 Fragments arranged by size: 2056 1525 631 605 527 156 122 107 71 BglII A'GATC_T Cuts at: 2272 5795 2272 Size: 3523 2277 BlpI GC'TnA_GC Cuts at: 3217 3217 Size: 5800 Bme1580I G_kGCm'C Cuts at: 1790 2168 2372 2391 2450 3861 5017 1790 Size: 378 204 19 59 1411 1156 2573 Fragments arranged by size: 2573 1411 1156 378 204 59 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5800 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2375 3531 4489 Size: 416 53 83 399 24 958 1156 958 Cuts at: 4489 442 Size: 1753 Fragments arranged by size: 1753 1156 958 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5800 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2629 2629 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2594 2615 2865 4460 1239 Size: 1355 21 250 1595 2579 Fragments arranged by size: 2579 1595 1355 250 21 Bpu10I CC'TnA_GC Cuts at: 2022 2180 2022 Size: 158 5642 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2423 3925 4790 5088 2423 Size: 1502 865 298 3135 Fragments arranged by size: 3135 1502 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2258 4463 882 Size: 617 759 2205 2219 Fragments arranged by size: 2219 2205 759 617 BsaAI yAC'GTr Cuts at: 493 1204 3591 493 Size: 711 2387 2702 Fragments arranged by size: 2702 2387 711 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3607 3989 4624 275 Size: 53 83 186 936 2074 382 635 1451 Fragments arranged by size: 2074 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2689 4233 4631 4905 5052 5463 2689 Size: 1544 398 274 147 411 3026 Fragments arranged by size: 3026 1544 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2211 2241 1178 Size: 30 165 30 808 30 4737 Fragments arranged by size: 4737 808 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2432 2967 2992 1186 Size: 1246 535 25 3994 Fragments arranged by size: 3994 1246 535 25 BseYI C'CCAG_C Cuts at: 2035 2161 2353 2636 5003 5499 2035 Size: 126 192 283 2367 496 2336 Fragments arranged by size: 2367 2336 496 283 192 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2855 1163 Size: 573 1119 4108 Fragments arranged by size: 4108 1119 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3250 4011 5039 1540 Size: 275 186 1249 761 1028 2301 Fragments arranged by size: 2301 1249 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2529 2571 2817 2901 2959 3861 3946 5017 2529 Size: 42 246 84 58 902 85 1071 3312 Fragments arranged by size: 3312 1071 902 246 85 84 58 42 BsmI GAATG_Cn' Cuts at: 3311 3404 3311 Size: 93 5707 BsmBI CGTCTCn'nnnn_ Cuts at: 3009 3009 Size: 5800 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 2168 2214 2372 2391 2450 2529 Size: 399 253 125 46 158 19 59 79 Cuts at: 2529 2571 2610 2817 2901 2959 3119 3861 3946 Size: 42 39 207 84 58 160 742 85 Cuts at: 3946 5017 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 253 207 160 158 125 85 84 79 59 58 46 42 39 19 BspEI T'CCGG_A Cuts at: 2689 5463 2689 Size: 2774 3026 BspHI T'CATG_A Cuts at: 2424 3689 5419 2424 Size: 1265 1730 2805 Fragments arranged by size: 2805 1730 1265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2672 3155 844 Size: 1828 483 3489 Fragments arranged by size: 3489 1828 483 BsrBI CCG'CTC Cuts at: 1394 1823 3687 1394 Size: 429 1864 3507 Fragments arranged by size: 3507 1864 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4295 4469 65 Size: 1183 3047 174 1396 Fragments arranged by size: 3047 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2735 4444 4631 1700 Size: 110 925 1709 187 2869 Fragments arranged by size: 2869 1709 925 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5800 BssSI C'ACGA_G Cuts at: 2900 3860 4872 2900 Size: 960 1012 3828 Fragments arranged by size: 3828 1012 960 BstAPI GCAn_nnn'nTGC Cuts at: 794 2350 2560 794 Size: 1556 210 4034 Fragments arranged by size: 4034 1556 210 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstXI CCAn_nnnn'nTGG Cuts at: 2997 2997 Size: 5800 BstYI r'GATC_y Cuts at: 1972 2272 2768 3000 3130 3497 3882 3899 5351 Size: 300 496 232 130 367 385 17 1452 Cuts at: 5351 5437 5449 5795 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 496 385 367 346 300 232 130 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2861 2861 Size: 5800 BtgI C'CryG_G Cuts at: 513 1065 2004 2015 2109 2584 513 Size: 552 939 11 94 475 3729 Fragments arranged by size: 3729 939 552 475 94 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2823 487 Size: 683 56 1597 3464 Fragments arranged by size: 3464 1597 683 56 BtsI GCAGTG_nn' Cuts at: 3316 4110 4130 3316 Size: 794 20 4986 Fragments arranged by size: 4986 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3026 4807 809 Size: 507 1710 1781 1802 Fragments arranged by size: 1802 1781 1710 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2514 2724 2737 Size: 54 1425 50 275 18 684 210 13 Cuts at: 2737 3247 4136 5765 8 Size: 510 889 1629 43 Fragments arranged by size: 1629 1425 889 684 510 275 210 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 3247 1537 Size: 275 1435 4090 Fragments arranged by size: 4090 1435 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3730 1353 Size: 2377 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2222 4387 4761 4907 387 Size: 1064 138 633 2165 374 146 1280 Fragments arranged by size: 2165 1280 1064 633 374 146 138 EcoICRI GAG'CTC Cuts at: 2527 2957 2527 Size: 430 5370 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2594 2615 2645 2865 3863 4460 5247 Size: 393 962 21 30 220 998 597 787 Cuts at: 5247 5515 1239 Size: 268 1524 Fragments arranged by size: 1524 998 962 787 597 393 268 220 30 21 EcoNI CCTnn'n_nnAGG Cuts at: 2441 2441 Size: 5800 EcoO109I rG'GnC_Cy Cuts at: 2011 2330 2347 2369 2473 3028 2011 Size: 319 17 22 104 555 4783 Fragments arranged by size: 4783 555 319 104 22 17 EcoRI G'AATT_C Cuts at: 3109 3487 3109 Size: 378 5422 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 2036 2673 4947 1678 Size: 358 637 2274 2531 Fragments arranged by size: 2531 2274 637 358 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2211 2243 2257 2289 2366 Size: 32 135 32 639 32 14 32 77 Cuts at: 2366 2398 2861 2893 4441 4473 4515 4547 1373 Size: 32 463 32 1548 32 42 32 2626 Fragments arranged by size: 2626 1548 639 463 135 77 42 32 32 32 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 669 1657 1938 3144 3390 669 Size: 988 281 1206 246 3079 Fragments arranged by size: 3079 1206 988 281 246 HindIII A'AGCT_T Cuts at: 748 2304 2313 748 Size: 1556 9 4235 Fragments arranged by size: 4235 1556 9 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KpnI G_GTAC'C Cuts at: 3125 3125 Size: 5800 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4914 5098 1305 Size: 3609 184 2007 Fragments arranged by size: 3609 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3978 Fragments arranged by size: 3978 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 3758 4117 4276 518 Size: 648 1218 1374 359 159 2042 Fragments arranged by size: 2042 1374 1218 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2111 2491 2586 2729 2942 3216 3893 Size: 411 94 380 95 143 213 274 677 Cuts at: 3893 5041 5286 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 411 380 274 245 213 143 95 94 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4309 Fragments arranged by size: 4309 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5800 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 3158 4703 1132 Size: 97 1929 1545 2229 Fragments arranged by size: 2229 1929 1545 97 PasI CC'CwG_GG Cuts at: 1348 2028 3044 1348 Size: 680 1016 4104 Fragments arranged by size: 4104 1016 680 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5800 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2011 2330 3028 2011 Size: 319 698 4783 Fragments arranged by size: 4783 698 319 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PspOMI G'GGCC_C Cuts at: 2164 2368 2164 Size: 204 5596 PstI C_TGCA'G Cuts at: 830 2346 2669 3152 830 Size: 1516 323 483 3478 Fragments arranged by size: 3478 1516 483 323 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 SacI G_AGCT'C Cuts at: 2529 2959 2529 Size: 430 5370 SacII CC_GC'GG Cuts at: 2018 2112 2587 2018 Size: 94 475 5231 Fragments arranged by size: 5231 475 94 SalI G'TCGA_C Cuts at: 1936 3142 1936 Size: 1206 4594 SanDI GG'GwC_CC Cuts at: 2011 2011 Size: 5800 SbfI CC_TGCA'GG Cuts at: 3152 3152 Size: 5800 ScaI AGT'ACT Cuts at: 4048 5760 4048 Size: 1712 4088 SexAI A'CCwGG_T Cuts at: 2238 2238 Size: 5800 SfcI C'TryA_G Cuts at: 826 1045 2342 2665 2946 3148 4283 4964 5155 Size: 219 1297 323 281 202 1135 681 191 Cuts at: 5155 826 Size: 1471 Fragments arranged by size: 1471 1297 1135 681 323 281 219 202 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 2130 3127 1872 Size: 258 997 4545 Fragments arranged by size: 4545 997 258 SmlI C'TyrA_G Cuts at: 820 1017 1943 2438 3904 4805 5067 820 Size: 197 926 495 1466 901 262 1553 Fragments arranged by size: 1553 1466 926 901 495 262 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5800 StyI C'CwwG_G Cuts at: 513 1065 2004 2996 3239 513 Size: 552 939 992 243 3074 Fragments arranged by size: 3074 992 939 552 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2137 3827 3844 3997 4182 4627 325 Size: 1255 557 1690 17 153 185 445 1498 Fragments arranged by size: 1690 1498 1255 557 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4046 5758 96 Size: 274 80 33 51 1443 2069 1712 138 Fragments arranged by size: 2069 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2965 2997 5760 5792 2965 Size: 32 2763 32 2973 Fragments arranged by size: 2973 2763 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5800 XbaI T'CTAG_A Cuts at: 3136 3136 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 2128 3125 1870 Size: 258 997 4545 Fragments arranged by size: 4545 997 258 XmnI GAAnn'nnTTC Cuts at: 3929 3929 Size: 5800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SapI SfiI SfoI SrfI SwaI XcmI