Restriction map for
pFN21AB6461
##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BpuAI BsaI
BseAI BsgI BsiHKAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KpnI MaeI
MfeI MluI MroI MseI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tru9I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 888 CGCG CG^CG
AflIII 482 ACACGT A^C[GA][CT]GT
AlwI 500 GGATC GGATC(4/5)
BalI 854 TGGCCA TGG^CCA
BbeI 832 GGCGCC GGCGC^C
BglI 644 GCCACACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 354 AGATCT A^GATCT
BsaBI 302 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 793 TCCGGT [AT]^CCGG[AT]
BsmAI 190 GTCTC GTCTC(1/5)
BsmBI 189 CGTCTC CGTCTC(1/5)
BspMI 456 GCAGGT ACCTGC(4/8)
BsrBI 95 CCGCTC CCGCTC(-3/-3)
BsrFI 649 ACCGGC [GA]^CCGG[CT]
BstAPI 479 GCAACACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 888 CGCG CG^CG
BstXI 203 CCAAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 354 AGATCT [GA]^GATC[CT]
Cfr10I 649 ACCGGC [GA]^CCGG[CT]
DraII 900 GGGACCT [GA]G^G[ACGT]CC[CT]
EagI 927 CGGCCG C^GGCCG
EarI 169 GAAGAG CTCTTC(1/4)
EclXI 927 CGGCCG C^GGCCG
Eco52I 927 CGGCCG C^GGCCG
EcoO109I 900 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 896 CCATGG C^C[AT][AT]GG
FauI 786 GCGGG CCCGC(4/6)
HaeII 832 GGCGCC [GA]GCGC^[CT]
HgaI 808 GACGC GACGC(5/10)
HindIII 398 AAGCTT A^AGCTT
KasI 832 GGCGCC G^GCGCC
Ksp632I 169 GAAGAG CTCTTC(1/4)
KspI 887 CCGCGG CCGC^GG
MamI 302 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 169 GAAGA GAAGA(8/7)
MflI 354 AGATCT [GA]^GATC[CT]
MluNI 854 TGGCCA TGG^CCA
MscI 854 TGGCCA TGG^CCA
MslI 220 CACCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 888 CGCG CG^CG
NarI 832 GGCGCC GG^CGCC
NcoI 896 CCATGG C^CATGG
PflMI 542 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 900 GGGACCT [GA]G^G[AT]CC[CT]
SacII 887 CCGCGG CCGC^GG
SfcI 723 CTACAG C^T[GA][CT]AG
SfoI 832 GGCGCC GGC^GCC
SmlI 194 CTCAAG C^T[CT][GA]AG
SphI 636 GCATGC GCATG^C
SspI 14 AATATT AAT^ATT
StyI 896 CCATGG C^C[AT][AT]GG
TfiI 907 GATTC G^A[AT]TC
Van91I 542 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 354 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 808 GACGCC G[GA]^CG[CT]C
832 GGCGCC
BanI 538 GGCACC G^G[CT][GA]CC
832 GGCGCC
BbrPI 483 CACGTG CAC^GTG
715 CACGTG
BpmI 737 CTCCAG CTGGAG(16/14)
274 CTGGAG
BsaAI 483 CACGTG [CT]AC^GT[GA]
715 CACGTG
BsaHI 808 GACGCC G[GA]^CG[CT]C
832 GGCGCC
BstEII 87 GGTGACC G^GT[ACGT]ACC
226 GGTGACC
BstPI 87 GGTGACC G^GT[ACGT]ACC
226 GGTGACC
CfoI 238 GCGC GCG^C
833 GCGC
EaeI 854 TGGCCA [CT]^GGCC[GA]
927 CGGCCG
EcoO65I 87 GGTGACC G^GT[ACGT]ACC
226 GGTGACC
HhaI 238 GCGC GCG^C
833 GCGC
Hin1I 808 GACGCC G[GA]^CG[CT]C
832 GGCGCC
HinP1I 238 GCGC G^CGC
833 GCGC
NspI 636 GCATGC [GA]CATG^[CT]
859 ACATGC
PmaCI 483 CACGTG CAC^GTG
715 CACGTG
PmlI 483 CACGTG CAC^GTG
715 CACGTG
SfaNI 783 GATGC GCATC(5/9)
599 GCATC
TaqI 26 TCGA T^CGA
285 TCGA
Tsp509I 380 AATT ^AATT
505 AATT
TspRI 136 CACTG CA[GC]TG[ACGT][ACGT]^
547 CAGTG
TthHB8I 26 TCGA T^CGA
285 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanII
BbvI BcnI BmyI BsaJI BseRI
BsiEI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PvuII Sau3AI Sau96I ScrFI
TseI Tsp45I