(Circular) (Six-base) MAPSORT of: pFN21AB6461.seq Check: 3759 from: 1 to: 5668 pFN21AB6461 5668 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2452 2452 Size: 5668 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2989 2989 Size: 5668 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2391 2708 3731 5115 5383 1632 Size: 759 317 1023 1384 268 1917 Fragments arranged by size: 1917 1384 1023 759 317 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 2487 4567 5544 1203 Size: 1284 2080 977 1327 Fragments arranged by size: 2080 1327 1284 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 4397 4397 Size: 5668 AleI CACnn'nnGTG Cuts at: 1166 2229 1166 Size: 1063 4605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5668 AlwNI CAG_nnn'CTG Cuts at: 2278 2736 2927 3411 4983 2278 Size: 458 191 484 1572 2963 Fragments arranged by size: 2963 1572 484 458 191 ApaLI G'TGCA_C Cuts at: 3725 4881 3725 Size: 1156 4512 ApoI r'AATT_y Cuts at: 1493 2977 3203 3355 5464 1493 Size: 1484 226 152 2109 1697 Fragments arranged by size: 2109 1697 1484 226 152 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2993 1870 Size: 73 1050 4545 Fragments arranged by size: 4545 1050 73 BamHI G'GATC_C Cuts at: 2998 3365 2998 Size: 367 5301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2543 2837 2989 4444 618 Size: 325 217 627 756 294 152 1455 1842 Fragments arranged by size: 1842 1455 756 627 325 294 217 152 BanII G_rGCy'C Cuts at: 1391 2743 2754 2798 2987 1391 Size: 1352 11 44 189 4072 Fragments arranged by size: 4072 1352 189 44 11 BbeI G_GCGC'C Cuts at: 2841 2841 Size: 5668 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4697 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3859 3893 1903 Size: 34 1922 34 3678 Fragments arranged by size: 3678 1922 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 1548 Size: 5668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2655 4279 136 Size: 107 122 71 631 605 983 1624 1525 Fragments arranged by size: 1624 1525 983 631 605 122 107 71 BglII A'GATC_T Cuts at: 2359 5663 2359 Size: 3304 2364 BlpI GC'TnA_GC Cuts at: 3085 3085 Size: 5668 Bme1580I G_kGCm'C Cuts at: 1790 2495 2546 3729 4885 1790 Size: 705 51 1183 1156 2573 Fragments arranged by size: 2573 1183 1156 705 51 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5668 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2152 2248 2746 Size: 416 53 83 399 24 735 96 498 Cuts at: 2746 3399 4357 442 Size: 653 958 1753 Fragments arranged by size: 1753 958 735 653 498 416 399 96 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5668 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 2727 4328 1239 Size: 1061 427 1601 2579 Fragments arranged by size: 2579 1601 1061 427 Bpu10I CC'TnA_GC Cuts at: 2447 2447 Size: 5668 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2184 3793 4658 4956 2184 Size: 1609 865 298 2896 Fragments arranged by size: 2896 1609 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4331 882 Size: 617 2832 2219 Fragments arranged by size: 2832 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2490 2722 3459 493 Size: 711 1286 232 737 2702 Fragments arranged by size: 2702 1286 737 711 232 BsaBI GATnn'nnATC Cuts at: 2311 5662 2311 Size: 3351 2317 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2814 2838 3475 3857 Size: 53 83 186 936 1281 24 637 382 Cuts at: 3857 4492 275 Size: 635 1451 Fragments arranged by size: 1451 1281 936 637 635 382 186 83 53 24 BsaWI w'CCGG_w Cuts at: 2798 4101 4499 4773 4920 5331 2798 Size: 1303 398 274 147 411 3135 Fragments arranged by size: 3135 1303 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2038 2068 2642 2672 1178 Size: 30 165 30 635 30 574 30 4174 Fragments arranged by size: 4174 635 574 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2447 2633 1186 Size: 862 399 186 4221 Fragments arranged by size: 4221 862 399 186 BseYI C'CCAG_C Cuts at: 2083 2111 2833 4871 5367 2083 Size: 28 722 2038 496 2384 Fragments arranged by size: 2384 2038 722 496 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5095 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2803 2827 2935 3118 3879 4907 Size: 275 186 802 24 108 183 761 1028 Cuts at: 4907 1540 Size: 2301 Fragments arranged by size: 2301 1028 802 761 275 186 183 108 24 BsiHKAI G_wGCw'C Cuts at: 3729 3814 4885 3729 Size: 85 1071 4512 Fragments arranged by size: 4512 1071 85 BsmI GAATG_Cn' Cuts at: 3179 3272 3179 Size: 93 5575 BsmBI CGTCTCn'nnnn_ Cuts at: 2200 2200 Size: 5668 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2495 2546 2743 2754 2798 2987 3729 Size: 399 705 51 197 11 44 189 742 Cuts at: 3729 3814 4885 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 705 399 197 189 85 51 44 11 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 3557 5287 3557 Size: 1730 3938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2452 3023 844 Size: 1608 571 3489 Fragments arranged by size: 3489 1608 571 BsrBI CCG'CTC Cuts at: 1394 1823 2102 3555 1394 Size: 429 279 1453 3507 Fragments arranged by size: 3507 1453 429 279 BsrDI GCAATG_nn' Cuts at: 65 1248 4163 4337 65 Size: 1183 2915 174 1396 Fragments arranged by size: 2915 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2654 4312 4499 1700 Size: 110 844 1658 187 2869 Fragments arranged by size: 2869 1658 844 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5668 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 3728 4740 3728 Size: 1012 4656 BstAPI GCAn_nnn'nTGC Cuts at: 794 2490 794 Size: 1696 3972 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 2092 2231 1184 Size: 908 139 4621 Fragments arranged by size: 4621 908 139 BstXI CCAn_nnnn'nTGG Cuts at: 2215 2215 Size: 5668 BstYI r'GATC_y Cuts at: 1972 2359 2998 3365 3750 3767 5219 5305 5317 Size: 387 639 367 385 17 1452 86 12 Cuts at: 5317 5663 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 639 387 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2227 2892 2901 513 Size: 552 939 223 665 9 3280 Fragments arranged by size: 3280 939 665 552 223 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4929 Fragments arranged by size: 4929 683 56 BtsI GCAGTG_nn' Cuts at: 2558 3184 3978 3998 2558 Size: 626 794 20 4228 Fragments arranged by size: 4228 794 626 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5633 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4675 809 Size: 507 3359 1802 Fragments arranged by size: 3359 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2859 2932 3115 Size: 54 1425 50 275 18 1029 73 183 Cuts at: 3115 4004 5633 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1029 889 275 183 73 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2932 3115 1537 Size: 275 1120 183 4090 Fragments arranged by size: 4090 1120 275 183 EarI CTCTTCn'nnn_ Cuts at: 1353 2169 3598 1353 Size: 816 1429 3423 Fragments arranged by size: 3423 1429 816 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4255 4629 4775 387 Size: 1064 138 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2391 2708 2727 3731 4328 5115 Size: 393 668 91 317 19 1004 597 787 Cuts at: 5115 5383 1239 Size: 268 1524 Fragments arranged by size: 1524 1004 787 668 597 393 317 268 91 19 EcoO109I rG'GnC_Cy Cuts at: 2906 2906 Size: 5668 EcoRI G'AATT_C Cuts at: 2977 3355 2977 Size: 378 5290 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 2841 4815 1678 Size: 1163 1974 2531 Fragments arranged by size: 2531 1974 1163 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2086 2118 4309 4341 4383 Size: 32 135 32 514 32 2191 32 42 Cuts at: 4383 4415 1373 Size: 32 2626 Fragments arranged by size: 2626 2191 514 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3258 669 Size: 988 281 1074 246 3079 Fragments arranged by size: 3079 1074 988 281 246 HindIII A'AGCT_T Cuts at: 748 2403 748 Size: 1655 4013 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KasI G'GCGC_C Cuts at: 2837 2837 Size: 5668 KpnI G_GTAC'C Cuts at: 2993 2993 Size: 5668 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2539 4782 4966 1305 Size: 1234 2243 184 2007 Fragments arranged by size: 2243 2007 1234 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2861 10 Size: 54 1425 343 1029 2817 Fragments arranged by size: 2817 1425 1029 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2229 3626 3985 4144 518 Size: 648 1063 1397 359 159 2042 Fragments arranged by size: 2042 1397 1063 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2170 2472 2596 2857 2894 3084 3761 4909 Size: 564 302 124 261 37 190 677 1148 Cuts at: 4909 5154 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 564 302 261 245 190 124 37 NarI GG'CG_CC Cuts at: 2838 2838 Size: 5668 NcoI C'CATG_G Cuts at: 513 1065 2004 2901 513 Size: 552 939 897 3280 Fragments arranged by size: 3280 939 897 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5668 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5668 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NspI r_CATG'y Cuts at: 1132 1229 2645 2868 3026 4571 1132 Size: 97 1416 223 158 1545 2229 Fragments arranged by size: 2229 1545 1416 223 158 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5668 PciI A'CATG_T Cuts at: 4567 4567 Size: 5668 PflMI CCAn_nnn'nTGG Cuts at: 1108 2553 1108 Size: 1445 4223 PfoI T'CCnGG_A Cuts at: 2333 2427 2660 2333 Size: 94 233 5341 Fragments arranged by size: 5341 233 94 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PmlI CAC'GTG Cuts at: 2490 2722 2490 Size: 232 5436 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3737 3769 5250 5282 3737 Size: 32 1481 32 4123 Fragments arranged by size: 4123 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2906 2906 Size: 5668 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5668 PsiI TTA'TAA Cuts at: 3238 3238 Size: 5668 PstI C_TGCA'G Cuts at: 830 3020 830 Size: 2190 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 PvuII CAG'CTG Cuts at: 2170 2596 2857 2170 Size: 426 261 4981 Fragments arranged by size: 4981 426 261 SacII CC_GC'GG Cuts at: 2895 2895 Size: 5668 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SfcI C'TryA_G Cuts at: 826 1045 2728 3016 4151 4832 5023 826 Size: 219 1683 288 1135 681 191 1471 Fragments arranged by size: 1683 1471 1135 681 288 219 191 SfoI GGC'GCC Cuts at: 2839 2839 Size: 5668 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2995 1872 Size: 1123 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2199 3772 4673 4935 820 Size: 197 926 256 1573 901 262 1553 Fragments arranged by size: 1573 1553 926 901 262 256 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 2645 3026 1132 Size: 1513 381 3774 Fragments arranged by size: 3774 1513 381 SspI AAT'ATT Cuts at: 5 52 2021 3592 5 Size: 47 1969 1571 2081 Fragments arranged by size: 2081 1969 1571 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5668 StyI C'CwwG_G Cuts at: 513 1065 2004 2901 3107 513 Size: 552 939 897 206 3074 Fragments arranged by size: 3074 939 897 552 206 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2695 2833 3695 3712 3865 4050 4495 Size: 1255 1115 138 862 17 153 185 445 Cuts at: 4495 325 Size: 1498 Fragments arranged by size: 1498 1255 1115 862 445 185 153 138 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3914 5626 96 Size: 274 80 33 51 1443 1937 1712 138 Fragments arranged by size: 1937 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2642 2674 5628 5660 2642 Size: 32 2954 32 2650 Fragments arranged by size: 2954 2650 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5668 XbaI T'CTAG_A Cuts at: 3004 3004 Size: 5668 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2993 1870 Size: 1123 4545 XmnI GAAnn'nnTTC Cuts at: 3797 3797 Size: 5668 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI