Restriction map for
pFN21AB6461
(Linear) (Six-base) MAPSORT of: KIBB6461.seq Check: 770 from: 1 to: 963
KIBB6461.orf
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 447 963
Size: 447 516
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 703 963
Size: 386 317 260
Fragments arranged by size:
386 317 260
AflIII A'CryG_T
Cuts at: 0 482 963
Size: 482 481
AleI CACnn'nnGTG
Cuts at: 0 224 963
Size: 224 739
AlwNI CAG_nnn'CTG
Cuts at: 0 273 731 922 963
Size: 273 458 191 41
Fragments arranged by size:
458 273 191 41
BanI G'GyrC_C
Cuts at: 0 538 832 963
Size: 538 294 131
Fragments arranged by size:
538 294 131
BanII G_rGCy'C
Cuts at: 0 738 749 793 963
Size: 738 11 44 170
Fragments arranged by size:
738 170 44 11
BbeI G_GCGC'C
Cuts at: 0 836 963
Size: 836 127
BglI GCCn_nnn'nGGC
Cuts at: 0 650 963
Size: 650 313
BglII A'GATC_T
Cuts at: 0 354 963
Size: 354 609
Bme1580I G_kGCm'C
Cuts at: 0 490 541 963
Size: 490 51 422
Fragments arranged by size:
490 422 51
BmrI ACTGGGnnnn_n'
Cuts at: 0 147 243 741 963
Size: 147 96 498 222
Fragments arranged by size:
498 222 147 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 722 963
Size: 295 427 241
Fragments arranged by size:
427 295 241
Bpu10I CC'TnA_GC
Cuts at: 0 442 963
Size: 442 521
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 963
Size: 179 784
BsaAI yAC'GTr
Cuts at: 0 485 717 963
Size: 485 232 246
Fragments arranged by size:
485 246 232
BsaBI GATnn'nnATC
Cuts at: 0 306 963
Size: 306 657
BsaHI Gr'CG_yC
Cuts at: 0 809 833 963
Size: 809 24 130
Fragments arranged by size:
809 130 24
BsaWI w'CCGG_w
Cuts at: 0 793 963
Size: 793 170
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 33 63 637 667 963
Size: 33 30 574 30 296
Fragments arranged by size:
574 296 33 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 442 628 963
Size: 43 399 186 335
Fragments arranged by size:
399 335 186 43
BseYI C'CCAG_C
Cuts at: 0 78 106 828 963
Size: 78 28 722 135
Fragments arranged by size:
722 135 78 28
BsiEI CG_ry'CG
Cuts at: 0 798 822 930 963
Size: 798 24 108 33
Fragments arranged by size:
798 108 33 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 195 963
Size: 195 768
Bsp1286I G_dGCh'C
Cuts at: 0 490 541 738 749 793 963
Size: 490 51 197 11 44 170
Fragments arranged by size:
490 197 170 51 44 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 447 963
Size: 447 516
BsrBI CCG'CTC
Cuts at: 0 97 963
Size: 97 866
BsrFI r'CCGG_y
Cuts at: 0 649 963
Size: 649 314
BstAPI GCAn_nnn'nTGC
Cuts at: 0 485 963
Size: 485 478
BstEII G'GTnAC_C
Cuts at: 0 87 226 963
Size: 87 139 737
Fragments arranged by size:
737 139 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 963
Size: 210 753
BstYI r'GATC_y
Cuts at: 0 354 963
Size: 354 609
BtgI C'CryG_G
Cuts at: 0 222 887 896 963
Size: 222 665 9 67
Fragments arranged by size:
665 222 67 9
BtsI GCAGTG_nn'
Cuts at: 0 553 963
Size: 553 410
EaeI y'GGCC_r
Cuts at: 0 854 927 963
Size: 854 73 36
Fragments arranged by size:
854 73 36
EagI C'GGCC_G
Cuts at: 0 927 963
Size: 927 36
EarI CTCTTCn'nnn_
Cuts at: 0 164 963
Size: 164 799
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 386 703 722 963
Size: 295 91 317 19 241
Fragments arranged by size:
317 295 241 91 19
EcoO109I rG'GnC_Cy
Cuts at: 0 901 963
Size: 901 62
HaeII r_GCGC'y
Cuts at: 0 836 963
Size: 836 127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 81 113 963
Size: 81 32 850
Fragments arranged by size:
850 81 32
HindIII A'AGCT_T
Cuts at: 0 398 963
Size: 398 565
KasI G'GCGC_C
Cuts at: 0 832 963
Size: 832 131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 963
Size: 534 429
MscI TGG'CCA
Cuts at: 0 856 963
Size: 856 107
MslI CAynn'nnrTG
Cuts at: 0 224 963
Size: 224 739
MspA1I CmG'CkG
Cuts at: 0 165 467 591 852 889 963
Size: 165 302 124 261 37 74
Fragments arranged by size:
302 261 165 124 74 37
NarI GG'CG_CC
Cuts at: 0 833 963
Size: 833 130
NcoI C'CATG_G
Cuts at: 0 896 963
Size: 896 67
NspI r_CATG'y
Cuts at: 0 640 863 963
Size: 640 223 100
Fragments arranged by size:
640 223 100
PflMI CCAn_nnn'nTGG
Cuts at: 0 548 963
Size: 548 415
PfoI T'CCnGG_A
Cuts at: 0 328 422 655 963
Size: 328 94 233 308
Fragments arranged by size:
328 308 233 94
PmlI CAC'GTG
Cuts at: 0 485 717 963
Size: 485 232 246
Fragments arranged by size:
485 246 232
PpuMI rG'GwC_Cy
Cuts at: 0 901 963
Size: 901 62
PvuII CAG'CTG
Cuts at: 0 165 591 852 963
Size: 165 426 261 111
Fragments arranged by size:
426 261 165 111
SacII CC_GC'GG
Cuts at: 0 890 963
Size: 890 73
SfcI C'TryA_G
Cuts at: 0 723 963
Size: 723 240
SfoI GGC'GCC
Cuts at: 0 834 963
Size: 834 129
SmlI C'TyrA_G
Cuts at: 0 194 963
Size: 194 769
SphI G_CATG'C
Cuts at: 0 640 963
Size: 640 323
SspI AAT'ATT
Cuts at: 0 16 963
Size: 16 947
StyI C'CwwG_G
Cuts at: 0 896 963
Size: 896 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 690 828 963
Size: 690 138 135
Fragments arranged by size:
690 138 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 637 669 963
Size: 637 32 294
Fragments arranged by size:
637 294 32
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI BanI BanII BbeI
BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsmBI
Bsp1286I BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI
BtgI BtsI EaeI EagI EarI Eco57MI EcoO109I HaeII
Hin4I HindIII KasI MmeI MscI MslI MspA1I NarI
NcoI NspI PflMI PfoI PmlI PpuMI PvuII SacII
SfcI SfoI SmlI SphI SspI StyI TaqII TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BlpI BmgBI BmtI BplI BsaI BsgI
BsiHKAI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EciI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI KpnI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI TatI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI