Restriction map for
pFN21AB8635
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaWI BseAI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MseI MspA1I
MunI NcoI NdeI NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuII RcaI RsaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 369 GGCGCC G[GA]^CG[CT]C
AflIII 104 ACGTGT A^C[GA][CT]GT
AlwNI 81 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 183 GGGCCC GGGCC^C
BanI 369 GGCGCC G^G[CT][GA]CC
BbeI 369 GGCGCC GGCGC^C
BcgI 376 GCACCGTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 52 CTAG C^TAG
BsaHI 369 GGCGCC G[GA]^CG[CT]C
BsaI 532 GAGACC GGTCTC(1/5)
BsiEI 395 CGATCG CG[GA][CT]^CG
BsmAI 532 GAGAC GTCTC(1/5)
BsrBI 650 CCGCTC CCGCTC(-3/-3)
BsrI 251 ACTGG ACTGG(1/-1)
BssHII 490 GCGCGC G^CGCGC
BssSI 292 CACGAG CACGAG(-5/-1)
BstF5I 708 GGATG GGATG(2/0)
DraIII 100 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 355 CCACGG C^C[GA][CT]GG
EcoNI 51 CCTAGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 708 GGATG GGATG(9/13)
HaeII 369 GGCGCC [GA]GCGC^[CT]
HgaI 680 GCGTC GACGC(5/10)
Hin1I 369 GGCGCC G[GA]^CG[CT]C
HinfI 390 GACTC G^A[ACGT]TC
KasI 369 GGCGCC G^GCGCC
MaeI 52 CTAG C^TAG
MaeII 104 ACGT A^CGT
MaeIII 469 GTGAC ^GT[ACGT]AC
MslI 416 CACCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 369 GGCGCC GG^CGCC
PflMI 206 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 390 GACTC GAGTC(4/5)
PstI 282 CTGCAG CTGCA^G
PvuI 395 CGATCG CGAT^CG
SapI 69 GAAGAGC GCTCTTC(1/4)
SfcI 282 CTGCAG C^T[GA][CT]AG
SfoI 369 GGCGCC GGC^GCC
SmlI 256 CTCAAG C^T[CT][GA]AG
Tsp45I 469 GTGAC ^GT[GC]AC
TspRI 153 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 206 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AluI 14 AGCT AG^CT
95 AGCT
AvaI 384 CTCGGG C^[CT]CG[GA]G
462 CTCGGG
BanII 183 GGGCCC G[GA]GC[CT]^C
578 GAGCCC
BmyI 183 GGGCCC G[AGT]GC[ACT]^C
578 GAGCCC
BsoBI 384 CTCGGG C^[CT]CG[GA]G
462 CTCGGG
Bsp1286I 183 GGGCCC G[AGT]GC[ACT]^C
578 GAGCCC
BspMI 85 ACCTGC ACCTGC(4/8)
231 GCAGGT
BsrFI 287 GCCGGC [GA]^CCGG[CT]
540 GCCGGC
Cfr10I 287 GCCGGC [GA]^CCGG[CT]
540 GCCGGC
DpnII 396 GATC ^GATC
591 GATC
EarI 40 CTCTTC CTCTTC(1/4)
69 GAAGAG
HaeIII 184 GGCC GG^CC
477 GGCC
Ksp632I 40 CTCTTC CTCTTC(1/4)
69 GAAGAG
MboI 396 GATC ^GATC
591 GATC
MboII 69 GAAGA GAAGA(8/7)
41 TCTTC
NaeI 287 GCCGGC GCC^GGC
540 GCCGGC
NdeII 396 GATC ^GATC
591 GATC
NgoMIV 287 GCCGGC G^CCGGC
540 GCCGGC
Sau3AI 396 GATC ^GATC
591 GATC
SfaNI 709 GATGC GCATC(5/9)
278 GCATC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AvaII BbvI BcnI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmFI BssKI BstNI BstUI
Cac8I CfoI Cfr13I DraII EcoO109I
EcoRII FauI Fnu4HI HapII HhaI
HinP1I HpaII HphI ItaI MnlI
MspI MvaI MvnI MwoI NciI
NlaIV PpuMI Sau96I ScrFI TseI