(Circular) (Six-base) MAPSORT of: pFN21AB8635.seq Check: 1786 from: 1 to: 5428 pFN21AB8635 5428 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2227 2227 Size: 5428 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3238 278 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2771 1937 Size: 834 4594 Acc65I G'GTAC_C Cuts at: 2749 2749 Size: 5428 AclI AA'CG_TT Cuts at: 1563 3555 3928 1563 Size: 1992 373 3063 Fragments arranged by size: 3063 1992 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3491 4875 5143 1632 Size: 1859 1384 268 1917 Fragments arranged by size: 1917 1859 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5231 AflIII A'CryG_T Cuts at: 1203 2109 4327 5304 1203 Size: 906 2218 977 1327 Fragments arranged by size: 2218 1327 977 906 AgeI A'CCGG_T Cuts at: 4259 4259 Size: 5428 AhdI GACnn_n'nnGTC Cuts at: 4157 4157 Size: 5428 AleI CACnn'nnGTG Cuts at: 1166 2425 1166 Size: 1259 4169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5428 AlwNI CAG_nnn'CTG Cuts at: 2091 3171 4743 2091 Size: 1080 1572 2776 Fragments arranged by size: 2776 1572 1080 ApaI G_GGCC'C Cuts at: 2192 2192 Size: 5428 ApaLI G'TGCA_C Cuts at: 3485 4641 3485 Size: 1156 4272 ApoI r'AATT_y Cuts at: 1493 2737 2963 3115 5224 1493 Size: 1244 226 152 2109 1697 Fragments arranged by size: 2109 1697 1244 226 152 AseI AT'TA_AT Cuts at: 160 1032 3982 160 Size: 872 2950 1606 Fragments arranged by size: 2950 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 AvaI C'yCGr_G Cuts at: 1870 1943 2389 2467 2753 1870 Size: 73 446 78 286 4545 Fragments arranged by size: 4545 446 286 78 73 BamHI G'GATC_C Cuts at: 2758 3125 2758 Size: 367 5061 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2374 2749 4204 618 Size: 325 217 627 587 375 1455 1842 Fragments arranged by size: 1842 1455 627 587 375 325 217 BanII G_rGCy'C Cuts at: 1391 2192 2587 2747 1391 Size: 801 395 160 4072 Fragments arranged by size: 4072 801 395 160 BbeI G_GCGC'C Cuts at: 2378 2378 Size: 5428 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4457 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2370 2404 3619 3653 1903 Size: 34 50 34 349 34 1215 34 3678 Fragments arranged by size: 3678 1215 349 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3320 4530 3320 Size: 1210 4218 BclI T'GATC_A Cuts at: 1548 1548 Size: 5428 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4039 136 Size: 107 122 71 631 605 2367 1525 Fragments arranged by size: 2367 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5423 5423 Size: 5428 BlpI GC'TnA_GC Cuts at: 2845 2845 Size: 5428 Bme1580I G_kGCm'C Cuts at: 1790 2192 3489 4645 1790 Size: 402 1297 1156 2573 Fragments arranged by size: 2573 1297 1156 402 BmgBI CAC'GTC Cuts at: 1318 2110 1318 Size: 792 4636 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3159 4117 442 Size: 416 53 83 399 24 1742 958 1753 Fragments arranged by size: 1753 1742 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5428 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2209 2562 4088 1239 Size: 911 59 353 1526 2579 Fragments arranged by size: 2579 1526 911 353 59 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2246 3553 4418 4716 2246 Size: 1307 865 298 2958 Fragments arranged by size: 2958 1307 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2531 4091 882 Size: 617 1032 1560 2219 Fragments arranged by size: 2219 1560 1032 617 BsaAI yAC'GTr Cuts at: 493 1204 3219 493 Size: 711 2015 2702 Fragments arranged by size: 2702 2015 711 BsaBI GATnn'nnATC Cuts at: 5422 5422 Size: 5428 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2375 3235 3617 4252 Size: 53 83 186 936 842 860 382 635 Cuts at: 4252 275 Size: 1451 Fragments arranged by size: 1451 936 860 842 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3861 4259 4533 4680 5091 3861 Size: 398 274 147 411 4198 Fragments arranged by size: 4198 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2033 2063 2574 2604 1178 Size: 30 165 30 630 30 511 30 4002 Fragments arranged by size: 4002 630 511 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2312 2627 2649 1186 Size: 1126 315 22 3965 Fragments arranged by size: 3965 1126 315 22 BseYI C'CCAG_C Cuts at: 2016 2097 2531 4631 5127 2016 Size: 81 434 2100 496 2317 Fragments arranged by size: 2317 2100 496 434 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2024 2126 2255 2263 2364 1163 Size: 573 288 102 129 8 101 4227 Fragments arranged by size: 4227 573 288 129 102 101 8 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2403 2878 3639 4667 1540 Size: 275 186 402 475 761 1028 2301 Fragments arranged by size: 2301 1028 761 475 402 275 186 BsiHKAI G_wGCw'C Cuts at: 3489 3574 4645 3489 Size: 85 1071 4272 Fragments arranged by size: 4272 1071 85 BsmI GAATG_Cn' Cuts at: 2939 3032 2939 Size: 93 5335 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2192 2587 2747 3489 3574 4645 1391 Size: 399 402 395 160 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 402 399 395 160 85 BspEI T'CCGG_A Cuts at: 5091 5091 Size: 5428 BspHI T'CATG_A Cuts at: 3317 5047 3317 Size: 1730 3698 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2099 2227 2716 2783 844 Size: 1255 128 489 67 3489 Fragments arranged by size: 3489 1255 489 128 67 BsrBI CCG'CTC Cuts at: 1394 1823 2657 3315 1394 Size: 429 834 658 3507 Fragments arranged by size: 3507 834 658 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3923 4097 65 Size: 1183 2675 174 1396 Fragments arranged by size: 2675 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2292 2545 4072 4259 1700 Size: 110 482 253 1527 187 2869 Fragments arranged by size: 2869 1527 482 253 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5428 BssHII G'CGCG_C Cuts at: 1925 2495 1925 Size: 570 4858 BssSI C'ACGA_G Cuts at: 2297 3488 4500 2297 Size: 1191 1012 3225 Fragments arranged by size: 3225 1191 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5428 BstBI TT'CG_AA Cuts at: 4232 4232 Size: 5428 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5428 BstYI r'GATC_y Cuts at: 1972 2758 3125 3510 3527 4979 5065 5077 5423 Size: 786 367 385 17 1452 86 12 346 Cuts at: 5423 1972 Size: 1977 Fragments arranged by size: 1977 1452 786 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2360 513 Size: 552 1295 3581 Fragments arranged by size: 3581 1295 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4689 Fragments arranged by size: 4689 683 56 BtsI GCAGTG_nn' Cuts at: 2944 3738 3758 2944 Size: 794 20 4614 Fragments arranged by size: 4614 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5428 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5393 DraI TTT'AAA Cuts at: 2732 2732 Size: 5428 DraIII CAC_nnn'GTG Cuts at: 2110 2110 Size: 5428 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4435 809 Size: 507 3119 1802 Fragments arranged by size: 3119 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2875 3764 5393 Size: 54 1425 50 275 18 1045 889 1629 Cuts at: 5393 8 Size: 43 Fragments arranged by size: 1629 1425 1045 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2875 1537 Size: 275 1063 4090 Fragments arranged by size: 4090 1063 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2051 2069 3358 1353 Size: 698 18 1289 3423 Fragments arranged by size: 3423 1289 698 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2537 2726 4015 4389 4535 387 Size: 1064 138 948 189 1289 374 146 1280 Fragments arranged by size: 1289 1280 1064 948 374 189 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2209 2562 3491 4088 4875 5143 Size: 393 518 59 353 929 597 787 268 Cuts at: 5143 1239 Size: 1524 Fragments arranged by size: 1524 929 787 597 518 393 353 268 59 EcoNI CCTnn'n_nnAGG Cuts at: 2060 2060 Size: 5428 EcoO109I rG'GnC_Cy Cuts at: 2088 2119 2435 2088 Size: 31 316 5081 Fragments arranged by size: 5081 316 31 EcoRI G'AATT_C Cuts at: 2737 3115 2737 Size: 378 5050 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2194 2287 2194 Size: 93 5335 FspI TGC'GCA Cuts at: 3934 3934 Size: 5428 HaeII r_GCGC'y Cuts at: 1678 2378 4575 1678 Size: 700 2197 2531 Fragments arranged by size: 2531 2197 700 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2450 2482 4069 4101 4143 Size: 32 135 32 878 32 1587 32 42 Cuts at: 4143 4175 1373 Size: 32 2626 Fragments arranged by size: 2626 1587 878 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2772 3018 669 Size: 988 281 834 246 3079 Fragments arranged by size: 3079 988 834 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5428 HpaI GTT'AAC Cuts at: 3018 3018 Size: 5428 KasI G'GCGC_C Cuts at: 2374 2374 Size: 5428 KpnI G_GTAC'C Cuts at: 2753 2753 Size: 5428 MfeI C'AATT_G Cuts at: 3027 3027 Size: 5428 MluI A'CGCG_T Cuts at: 5304 5304 Size: 5428 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2389 4542 4726 1305 Size: 1084 2153 184 2007 Fragments arranged by size: 2153 2007 1084 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3606 Fragments arranged by size: 3606 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2425 3386 3745 3904 518 Size: 648 1259 961 359 159 2042 Fragments arranged by size: 2042 1259 961 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2844 3521 4669 4914 1606 Size: 1238 677 1148 245 2120 Fragments arranged by size: 2120 1238 1148 677 245 NaeI GCC'GGC Cuts at: 2294 2547 2294 Size: 253 5175 NarI GG'CG_CC Cuts at: 2375 2375 Size: 5428 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4876 NdeI CA'TA_TG Cuts at: 387 387 Size: 5428 NgoMIV G'CCGG_C Cuts at: 2292 2545 2292 Size: 253 5175 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5428 NotI GC'GGCC_GC Cuts at: 2875 2875 Size: 5428 NspI r_CATG'y Cuts at: 1132 1229 2786 4331 1132 Size: 97 1557 1545 2229 Fragments arranged by size: 2229 1557 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5428 PciI A'CATG_T Cuts at: 4327 4327 Size: 5428 PflMI CCAn_nnn'nTGG Cuts at: 1108 2217 1108 Size: 1109 4319 PfoI T'CCnGG_A Cuts at: 2551 2578 2551 Size: 27 5401 PmeI GTTT'AAAC Cuts at: 2732 2732 Size: 5428 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3497 3529 5010 5042 3497 Size: 32 1481 32 3883 Fragments arranged by size: 3883 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2088 2119 2435 2088 Size: 31 316 5081 Fragments arranged by size: 5081 316 31 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5428 PsiI TTA'TAA Cuts at: 2998 2998 Size: 5428 PspOMI G'GGCC_C Cuts at: 2188 2188 Size: 5428 PstI C_TGCA'G Cuts at: 830 2291 2780 830 Size: 1461 489 3478 Fragments arranged by size: 3478 1461 489 PvuI CG_AT'CG Cuts at: 2001 2403 2001 Size: 402 5026 SalI G'TCGA_C Cuts at: 1936 2770 1936 Size: 834 4594 SapI GCTCTTCn'nnn_ Cuts at: 2069 2069 Size: 5428 SbfI CC_TGCA'GG Cuts at: 2780 2780 Size: 5428 ScaI AGT'ACT Cuts at: 3676 5388 3676 Size: 1712 3716 SfcI C'TryA_G Cuts at: 826 1045 2287 2776 3911 4592 4783 826 Size: 219 1242 489 1135 681 191 1471 Fragments arranged by size: 1471 1242 1135 681 489 219 191 SfoI GGC'GCC Cuts at: 2376 2376 Size: 5428 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5428 SmaI CCC'GGG Cuts at: 1872 2755 1872 Size: 883 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2261 3532 4433 4695 820 Size: 197 926 318 1271 901 262 1553 Fragments arranged by size: 1553 1271 926 901 318 262 197 SnaBI TAC'GTA Cuts at: 493 3219 493 Size: 2726 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5428 SphI G_CATG'C Cuts at: 1132 2786 1132 Size: 1654 3774 SspI AAT'ATT Cuts at: 5 52 3352 5 Size: 47 3300 2081 Fragments arranged by size: 3300 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5428 StyI C'CwwG_G Cuts at: 513 1065 2867 513 Size: 552 1802 3074 Fragments arranged by size: 3074 1802 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2428 3455 3472 3625 3810 4255 325 Size: 1255 848 1027 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1027 848 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3674 5386 96 Size: 274 80 33 51 1443 1697 1712 138 Fragments arranged by size: 1712 1697 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2033 2065 2135 2167 2574 2606 5388 5420 2033 Size: 32 70 32 407 32 2782 32 2041 Fragments arranged by size: 2782 2041 407 70 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5428 XbaI T'CTAG_A Cuts at: 2764 2764 Size: 5428 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5428 XmaI C'CCGG_G Cuts at: 1870 2753 1870 Size: 883 4545 XmnI GAAnn'nnTTC Cuts at: 3557 3557 Size: 5428 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3236 276 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI