(Circular) (Six-base) MAPSORT of: pFN21AB6559.seq Check: 1901 from: 1 to: 5824 pFN21AB6559 5824 bp With 182 enzymes: SgfI * October 20, 2009 10:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2651 2651 Size: 5824 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3167 1937 Size: 1230 4594 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2376 2445 3887 5271 5539 1632 Size: 744 69 1442 1384 268 1917 Fragments arranged by size: 1917 1442 1384 744 268 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 2807 4723 5700 1203 Size: 1604 1916 977 1327 Fragments arranged by size: 1916 1604 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 2462 4553 2462 Size: 2091 3733 AleI CACnn'nnGTG Cuts at: 1166 2810 1166 Size: 1644 4180 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2634 1184 Size: 1450 4374 AlwNI CAG_nnn'CTG Cuts at: 2190 2522 2919 3567 5139 2190 Size: 332 397 648 1572 2875 Fragments arranged by size: 2875 1572 648 397 332 ApaI G_GGCC'C Cuts at: 2776 2776 Size: 5824 ApaLI G'TGCA_C Cuts at: 3881 5037 3881 Size: 1156 4668 ApoI r'AATT_y Cuts at: 1493 2897 3133 3359 3511 5620 1493 Size: 1404 236 226 152 2109 1697 Fragments arranged by size: 2109 1697 1404 236 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2159 2580 2940 3149 1870 Size: 73 216 421 360 209 4545 Fragments arranged by size: 4545 421 360 216 209 73 BamHI G'GATC_C Cuts at: 3021 3154 3521 3021 Size: 133 367 5324 Fragments arranged by size: 5324 367 133 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 3145 4600 618 Size: 325 217 627 269 1089 1455 1842 Fragments arranged by size: 1842 1455 1089 627 325 269 217 BanII G_rGCy'C Cuts at: 1391 2776 3042 3143 1391 Size: 1385 266 101 4072 Fragments arranged by size: 4072 1385 266 101 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2159 928 Size: 971 260 4593 Fragments arranged by size: 4593 971 260 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2679 2713 4015 4049 1903 Size: 34 742 34 1302 34 3678 Fragments arranged by size: 3678 1302 742 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3716 4926 3716 Size: 1210 4614 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BfrBI ATG'CAT Cuts at: 2973 2973 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4435 136 Size: 107 122 71 631 605 2763 1525 Fragments arranged by size: 2763 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 2776 3885 5041 1790 Size: 986 1109 1156 2573 Fragments arranged by size: 2573 1156 1109 986 BmgBI CAC'GTC Cuts at: 1318 2293 1318 Size: 975 4849 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2617 3555 4513 Size: 416 53 83 399 24 1200 938 958 Cuts at: 4513 442 Size: 1753 Fragments arranged by size: 1753 1200 958 938 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2322 2744 4484 1239 Size: 1083 422 1740 2579 Fragments arranged by size: 2579 1740 1083 422 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3949 4814 5112 3949 Size: 865 298 4661 Fragments arranged by size: 4661 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4487 882 Size: 617 2988 2219 Fragments arranged by size: 2988 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2634 2808 3615 493 Size: 711 1430 174 807 2702 Fragments arranged by size: 2702 1430 807 711 174 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3631 4013 4648 275 Size: 53 83 186 936 2098 382 635 1451 Fragments arranged by size: 2098 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4257 4655 4929 5076 5487 4257 Size: 398 274 147 411 4594 Fragments arranged by size: 4594 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5599 Fragments arranged by size: 5599 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2259 2303 2375 1186 Size: 1073 44 72 4635 Fragments arranged by size: 4635 1073 72 44 BseYI C'CCAG_C Cuts at: 2183 2446 2660 5027 5523 2183 Size: 263 214 2367 496 2484 Fragments arranged by size: 2484 2367 496 263 214 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2214 2396 1163 Size: 573 478 182 4591 Fragments arranged by size: 4591 573 478 182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3885 3970 5041 3885 Size: 85 1071 4668 Fragments arranged by size: 4668 1071 85 BsmI GAATG_Cn' Cuts at: 2975 3335 3428 2975 Size: 360 93 5371 Fragments arranged by size: 5371 360 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2776 3042 3143 3885 3970 5041 1391 Size: 399 986 266 101 742 85 1071 2174 Fragments arranged by size: 2174 1071 986 742 399 266 101 85 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2651 2825 3179 844 Size: 1807 174 354 3489 Fragments arranged by size: 3489 1807 354 174 BsrBI CCG'CTC Cuts at: 1394 1823 3711 1394 Size: 429 1888 3507 Fragments arranged by size: 3507 1888 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2391 4319 4493 65 Size: 1183 1143 1928 174 1396 Fragments arranged by size: 1928 1396 1183 1143 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2224 4468 4655 1700 Size: 110 414 2244 187 2869 Fragments arranged by size: 2869 2244 414 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 3090 794 Size: 2296 3528 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5824 BstXI CCAn_nnnn'nTGG Cuts at: 2660 2684 2660 Size: 24 5800 BstYI r'GATC_y Cuts at: 1972 3021 3154 3521 3906 3923 5375 5461 5473 Size: 1049 133 367 385 17 1452 86 12 Cuts at: 5473 5819 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1049 385 367 346 133 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2480 2480 Size: 5824 BtgI C'CryG_G Cuts at: 513 1065 2004 2079 2487 3042 513 Size: 552 939 75 408 555 3295 Fragments arranged by size: 3295 939 555 552 408 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2085 487 Size: 683 56 859 4226 Fragments arranged by size: 4226 859 683 56 BtsI GCAGTG_nn' Cuts at: 3340 4134 4154 3340 Size: 794 20 5010 Fragments arranged by size: 5010 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2122 3271 Size: 54 1425 50 275 18 246 46 1149 Cuts at: 3271 4160 5789 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1149 889 275 246 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2249 2352 2490 2600 3083 3754 1353 Size: 665 231 103 138 110 483 671 3423 Fragments arranged by size: 3423 671 665 483 231 138 110 103 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2099 4411 4785 4931 387 Size: 1064 138 510 2312 374 146 1280 Fragments arranged by size: 2312 1280 1064 510 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2322 2376 2445 2744 3887 4484 5271 Size: 393 690 54 69 299 1143 597 787 Cuts at: 5271 5539 1239 Size: 268 1524 Fragments arranged by size: 1524 1143 787 690 597 393 299 268 69 54 EcoNI CCTnn'n_nnAGG Cuts at: 2780 2780 Size: 5824 EcoO109I rG'GnC_Cy Cuts at: 2382 2443 2456 2772 2773 2382 Size: 61 13 316 1 5433 Fragments arranged by size: 5433 316 61 13 1 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 4971 1678 Size: 3293 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2448 2480 4465 4497 4539 Size: 32 135 32 876 32 1985 32 42 Cuts at: 4539 4571 1373 Size: 32 2626 Fragments arranged by size: 2626 1985 876 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3168 3414 669 Size: 988 281 1230 246 3079 Fragments arranged by size: 3079 1230 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3423 3423 Size: 5824 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 5122 1305 Size: 3633 184 2007 Fragments arranged by size: 3633 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4002 Fragments arranged by size: 4002 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2810 3782 4141 4300 518 Size: 648 1644 972 359 159 2042 Fragments arranged by size: 2042 1644 972 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2410 2450 2656 3240 3917 5065 5310 1606 Size: 804 40 206 584 677 1148 245 2120 Fragments arranged by size: 2120 1148 804 677 584 245 206 40 NaeI GCC'GGC Cuts at: 2226 2226 Size: 5824 NcoI C'CATG_G Cuts at: 513 1065 2004 2487 513 Size: 552 939 483 3850 Fragments arranged by size: 3850 939 552 483 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NgoMIV G'CCGG_C Cuts at: 2224 2224 Size: 5824 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NsiI A_TGCA'T Cuts at: 2975 2975 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 3182 4727 1132 Size: 97 1953 1545 2229 Fragments arranged by size: 2229 1953 1545 97 PasI CC'CwG_GG Cuts at: 1348 2130 2776 2851 1348 Size: 782 646 75 4321 Fragments arranged by size: 4321 782 646 75 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 2190 1108 Size: 1082 4742 PfoI T'CCnGG_A Cuts at: 2277 2754 2277 Size: 477 5347 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PmlI CAC'GTG Cuts at: 2634 2808 2634 Size: 174 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2456 2456 Size: 5824 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2772 2772 Size: 5824 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2855 2887 2855 Size: 32 5792 PstI C_TGCA'G Cuts at: 830 2215 2915 3020 3176 830 Size: 1385 700 105 156 3478 Fragments arranged by size: 3478 1385 700 156 105 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 PvuII CAG'CTG Cuts at: 2410 2656 2410 Size: 246 5578 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SapI GCTCTTCn'nnn_ Cuts at: 2249 2490 2249 Size: 241 5583 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SfcI C'TryA_G Cuts at: 826 1045 2211 2890 2911 3001 3016 3172 4307 Size: 219 1166 679 21 90 15 156 1135 Cuts at: 4307 4988 5179 826 Size: 681 191 1471 Fragments arranged by size: 1471 1166 1135 681 679 219 191 156 90 21 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 3151 1872 Size: 1279 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3928 4829 5091 820 Size: 197 926 1985 901 262 1553 Fragments arranged by size: 1985 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 3182 1132 Size: 2050 3774 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 2786 1517 Size: 1269 4555 StyI C'CwwG_G Cuts at: 513 1065 2004 2487 3049 3263 513 Size: 552 939 483 562 214 3074 Fragments arranged by size: 3074 939 562 552 483 214 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3851 3868 4021 4206 4651 325 Size: 1255 2271 17 153 185 445 1498 Fragments arranged by size: 2271 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2871 4070 5782 Size: 274 80 33 51 1443 894 1199 1712 Cuts at: 5782 96 Size: 138 Fragments arranged by size: 1712 1443 1199 894 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5784 5816 5784 Size: 32 5792 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5824 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 3149 1870 Size: 1279 4545 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI PacI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI