(Circular) (Six-base) MAPSORT of: pFN21AE0129.seq Check: 488 from: 1 to: 5449 pFN21AE0129 5449 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2105 2274 2650 2105 Size: 169 376 4904 Fragments arranged by size: 4904 376 169 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3259 278 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2610 2792 1937 Size: 673 182 4594 Fragments arranged by size: 4594 673 182 Acc65I G'GTAC_C Cuts at: 2770 2770 Size: 5449 AclI AA'CG_TT Cuts at: 1563 3576 3949 1563 Size: 2013 373 3063 Fragments arranged by size: 3063 2013 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3512 4896 5164 1632 Size: 1880 1384 268 1917 Fragments arranged by size: 1917 1880 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5252 AflIII A'CryG_T Cuts at: 1203 4348 5325 1203 Size: 3145 977 1327 Fragments arranged by size: 3145 1327 977 AgeI A'CCGG_T Cuts at: 4280 4280 Size: 5449 AhdI GACnn_n'nnGTC Cuts at: 4178 4178 Size: 5449 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5449 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5449 AlwNI CAG_nnn'CTG Cuts at: 2266 3192 4764 2266 Size: 926 1572 2951 Fragments arranged by size: 2951 1572 926 ApaI G_GGCC'C Cuts at: 2051 2521 2051 Size: 470 4979 ApaLI G'TGCA_C Cuts at: 2081 3506 4662 2081 Size: 1425 1156 2868 Fragments arranged by size: 2868 1425 1156 ApoI r'AATT_y Cuts at: 1493 2758 2984 3136 5245 1493 Size: 1265 226 152 2109 1697 Fragments arranged by size: 2109 1697 1265 226 152 AseI AT'TA_AT Cuts at: 160 1032 4003 160 Size: 872 2971 1606 Fragments arranged by size: 2971 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 AvaI C'yCGr_G Cuts at: 1870 1943 2774 1870 Size: 73 831 4545 Fragments arranged by size: 4545 831 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2417 2450 2417 Size: 33 5416 BamHI G'GATC_C Cuts at: 2779 3146 2779 Size: 367 5082 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 2623 2770 4225 618 Size: 325 217 627 278 558 147 1455 1842 Fragments arranged by size: 1842 1455 627 558 325 278 217 147 BanII G_rGCy'C Cuts at: 1391 2051 2325 2521 2768 1391 Size: 660 274 196 247 4072 Fragments arranged by size: 4072 660 274 247 196 BbeI G_GCGC'C Cuts at: 2069 2069 Size: 5449 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2220 928 Size: 971 321 4157 Fragments arranged by size: 4157 971 321 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3640 3674 1903 Size: 34 1703 34 3678 Fragments arranged by size: 3678 1703 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3341 4551 3341 Size: 1210 4239 BclI T'GATC_A Cuts at: 1548 1548 Size: 5449 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2656 4060 136 Size: 107 122 71 631 605 984 1404 1525 Fragments arranged by size: 1525 1404 984 631 605 122 107 71 BglII A'GATC_T Cuts at: 5444 5444 Size: 5449 BlpI GC'TnA_GC Cuts at: 2866 2866 Size: 5449 Bme1580I G_kGCm'C Cuts at: 1790 2051 2085 2521 2628 3510 4666 1790 Size: 261 34 436 107 882 1156 2573 Fragments arranged by size: 2573 1156 882 436 261 107 34 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5449 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3180 4138 442 Size: 416 53 83 399 24 1763 958 1753 Fragments arranged by size: 1763 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5449 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2444 4109 1239 Size: 1205 1665 2579 Fragments arranged by size: 2579 1665 1205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3574 4439 4737 3574 Size: 865 298 4286 Fragments arranged by size: 4286 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2180 2416 4112 882 Size: 617 681 236 1696 2219 Fragments arranged by size: 2219 1696 681 617 236 BsaAI yAC'GTr Cuts at: 493 1204 3240 493 Size: 711 2036 2702 Fragments arranged by size: 2702 2036 711 BsaBI GATnn'nnATC Cuts at: 5443 5443 Size: 5449 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 2199 3256 3638 Size: 53 83 186 936 533 133 1057 382 Cuts at: 3638 4273 275 Size: 635 1451 Fragments arranged by size: 1451 1057 936 635 533 382 186 133 83 53 BsaWI w'CCGG_w Cuts at: 3882 4280 4554 4701 5112 3882 Size: 398 274 147 411 4219 Fragments arranged by size: 4219 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2607 2637 1178 Size: 30 165 30 1204 30 3990 Fragments arranged by size: 3990 1204 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5449 BseYI C'CCAG_C Cuts at: 2568 4652 5148 2568 Size: 2084 496 2869 Fragments arranged by size: 2869 2084 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2390 1163 Size: 573 654 4222 Fragments arranged by size: 4222 654 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2899 3660 4688 1540 Size: 275 186 898 761 1028 2301 Fragments arranged by size: 2301 1028 898 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2085 2592 3510 3595 4666 2085 Size: 507 918 85 1071 2868 Fragments arranged by size: 2868 1071 918 507 85 BsmI GAATG_Cn' Cuts at: 2960 3053 2960 Size: 93 5356 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2085 2325 2521 2592 2628 2768 Size: 399 261 34 240 196 71 36 140 Cuts at: 2768 3510 3595 4666 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 261 240 196 140 85 71 36 34 BspEI T'CCGG_A Cuts at: 5112 5112 Size: 5449 BspHI T'CATG_A Cuts at: 3338 5068 3338 Size: 1730 3719 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2105 2274 2445 2596 2650 2804 844 Size: 1261 169 171 151 54 154 3489 Fragments arranged by size: 3489 1261 171 169 154 151 54 BsrBI CCG'CTC Cuts at: 1394 1823 3336 1394 Size: 429 1513 3507 Fragments arranged by size: 3507 1513 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2145 3944 4118 65 Size: 1183 897 1799 174 1396 Fragments arranged by size: 1799 1396 1183 897 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2062 2243 4093 4280 1700 Size: 110 252 181 1850 187 2869 Fragments arranged by size: 2869 1850 252 187 181 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5449 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5449 BssSI C'ACGA_G Cuts at: 2274 3509 4521 2274 Size: 1235 1012 3202 Fragments arranged by size: 3202 1235 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2266 794 Size: 1472 3977 BstBI TT'CG_AA Cuts at: 4253 4253 Size: 5449 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5449 BstYI r'GATC_y Cuts at: 1972 2742 2779 3146 3531 3548 5000 5086 5098 Size: 770 37 367 385 17 1452 86 12 Cuts at: 5098 5444 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 770 385 367 346 86 37 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2539 2580 2651 2730 513 Size: 552 939 535 41 71 79 3232 Fragments arranged by size: 3232 939 552 535 79 71 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2560 487 Size: 683 56 1334 3376 Fragments arranged by size: 3376 1334 683 56 BtsI GCAGTG_nn' Cuts at: 2151 2378 2965 3759 3779 2151 Size: 227 587 794 20 3821 Fragments arranged by size: 3821 794 587 227 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5449 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5414 DraI TTT'AAA Cuts at: 2753 2753 Size: 5449 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4456 809 Size: 507 3140 1802 Fragments arranged by size: 3140 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2241 2896 3785 Size: 54 1425 50 275 18 411 655 889 Cuts at: 3785 5414 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 655 411 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2896 1537 Size: 275 1084 4090 Fragments arranged by size: 4090 1084 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 3379 1353 Size: 897 1129 3423 Fragments arranged by size: 3423 1129 897 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2040 4036 4410 4556 387 Size: 1064 138 451 1996 374 146 1280 Fragments arranged by size: 1996 1280 1064 451 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2444 3512 4109 4896 5164 1239 Size: 393 812 1068 597 787 268 1524 Fragments arranged by size: 1524 1068 812 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2033 2047 2518 2033 Size: 14 471 4964 Fragments arranged by size: 4964 471 14 EcoRI G'AATT_C Cuts at: 2758 3136 2758 Size: 378 5071 FspI TGC'GCA Cuts at: 3955 3955 Size: 5449 HaeII r_GCGC'y Cuts at: 1678 2069 2549 4596 1678 Size: 391 480 2047 2531 Fragments arranged by size: 2531 2047 480 391 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4090 4122 4164 4196 1373 Size: 32 135 32 2518 32 42 32 2626 Fragments arranged by size: 2626 2518 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2793 3039 669 Size: 988 281 855 246 3079 Fragments arranged by size: 3079 988 855 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5449 HpaI GTT'AAC Cuts at: 3039 3039 Size: 5449 KasI G'GCGC_C Cuts at: 2065 2065 Size: 5449 KpnI G_GTAC'C Cuts at: 2774 2774 Size: 5449 MfeI C'AATT_G Cuts at: 3048 3048 Size: 5449 MluI A'CGCG_T Cuts at: 5325 5325 Size: 5449 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2319 4563 4747 1305 Size: 1014 2244 184 2007 Fragments arranged by size: 2244 2007 1014 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3627 Fragments arranged by size: 3627 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2288 2579 3407 3766 3925 518 Size: 648 1122 291 828 359 159 2042 Fragments arranged by size: 2042 1122 828 648 359 291 159 MspA1I CmG'CkG Cuts at: 1606 2484 2491 2865 3542 4690 4935 1606 Size: 878 7 374 677 1148 245 2120 Fragments arranged by size: 2120 1148 878 677 374 245 7 NaeI GCC'GGC Cuts at: 2064 2245 2064 Size: 181 5268 NarI GG'CG_CC Cuts at: 2066 2066 Size: 5449 NcoI C'CATG_G Cuts at: 513 1065 2004 2539 2730 513 Size: 552 939 535 191 3232 Fragments arranged by size: 3232 939 552 535 191 NdeI CA'TA_TG Cuts at: 387 387 Size: 5449 NgoMIV G'CCGG_C Cuts at: 2062 2243 2062 Size: 181 5268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5449 NotI GC'GGCC_GC Cuts at: 2896 2896 Size: 5449 NspI r_CATG'y Cuts at: 1132 1229 2807 4352 1132 Size: 97 1578 1545 2229 Fragments arranged by size: 2229 1578 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5449 PciI A'CATG_T Cuts at: 4348 4348 Size: 5449 PflMI CCAn_nnn'nTGG Cuts at: 1108 2449 1108 Size: 1341 4108 PfoI T'CCnGG_A Cuts at: 2355 2355 Size: 5449 PmeI GTTT'AAAC Cuts at: 2753 2753 Size: 5449 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3518 3550 5031 5063 3518 Size: 32 1481 32 3904 Fragments arranged by size: 3904 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5449 PsiI TTA'TAA Cuts at: 3019 3019 Size: 5449 PspOMI G'GGCC_C Cuts at: 2047 2517 2047 Size: 470 4979 PstI C_TGCA'G Cuts at: 830 2041 2607 2801 830 Size: 1211 566 194 3478 Fragments arranged by size: 3478 1211 566 194 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5449 PvuII CAG'CTG Cuts at: 2484 2484 Size: 5449 SalI G'TCGA_C Cuts at: 1936 2791 1936 Size: 855 4594 SapI GCTCTTCn'nnn_ Cuts at: 2250 2250 Size: 5449 SbfI CC_TGCA'GG Cuts at: 2607 2801 2607 Size: 194 5255 ScaI AGT'ACT Cuts at: 3697 5409 3697 Size: 1712 3737 SexAI A'CCwGG_T Cuts at: 2448 2448 Size: 5449 SfcI C'TryA_G Cuts at: 826 1045 2037 2603 2797 3932 4613 4804 826 Size: 219 992 566 194 1135 681 191 1471 Fragments arranged by size: 1471 1135 992 681 566 219 194 191 SfoI GGC'GCC Cuts at: 2067 2067 Size: 5449 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5449 SmaI CCC'GGG Cuts at: 1872 2776 1872 Size: 904 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3553 4454 4716 820 Size: 197 926 1610 901 262 1553 Fragments arranged by size: 1610 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3240 493 Size: 2747 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5449 SphI G_CATG'C Cuts at: 1132 2807 1132 Size: 1675 3774 SspI AAT'ATT Cuts at: 5 52 2701 3373 5 Size: 47 2649 672 2081 Fragments arranged by size: 2649 2081 672 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5449 StyI C'CwwG_G Cuts at: 513 1065 2004 2539 2730 2888 513 Size: 552 939 535 191 158 3074 Fragments arranged by size: 3074 939 552 535 191 158 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3476 3493 3646 3831 4276 325 Size: 1255 1896 17 153 185 445 1498 Fragments arranged by size: 1896 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3695 5407 96 Size: 274 80 33 51 1443 1718 1712 138 Fragments arranged by size: 1718 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2607 2639 5409 5441 2607 Size: 32 2770 32 2615 Fragments arranged by size: 2770 2615 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5449 XbaI T'CTAG_A Cuts at: 2785 2785 Size: 5449 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5449 XmaI C'CCGG_G Cuts at: 1870 2774 1870 Size: 904 4545 XmnI GAAnn'nnTTC Cuts at: 3578 3578 Size: 5449 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3257 276 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI