(Circular) (Six-base) MAPSORT of: pFN21AE0135.seq Check: 8912 from: 1 to: 5722 pFN21AE0135 5722 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3532 278 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3065 1937 Size: 1128 4594 Acc65I G'GTAC_C Cuts at: 3043 3043 Size: 5722 AclI AA'CG_TT Cuts at: 1563 3849 4222 1563 Size: 2286 373 3063 Fragments arranged by size: 3063 2286 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2596 2701 3785 5169 5437 1632 Size: 964 105 1084 1384 268 1917 Fragments arranged by size: 1917 1384 1084 964 268 105 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5525 AflIII A'CryG_T Cuts at: 1203 4621 5598 1203 Size: 3418 977 1327 Fragments arranged by size: 3418 1327 977 AgeI A'CCGG_T Cuts at: 4553 4553 Size: 5722 AhdI GACnn_n'nnGTC Cuts at: 4451 4451 Size: 5722 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5722 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2687 2939 1184 Size: 1503 252 3967 Fragments arranged by size: 3967 1503 252 AlwNI CAG_nnn'CTG Cuts at: 2619 2683 2973 3465 5037 2619 Size: 64 290 492 1572 3304 Fragments arranged by size: 3304 1572 492 290 64 ApaLI G'TGCA_C Cuts at: 3779 4935 3779 Size: 1156 4566 ApoI r'AATT_y Cuts at: 1493 3031 3257 3409 5518 1493 Size: 1538 226 152 2109 1697 Fragments arranged by size: 2109 1697 1538 226 152 AseI AT'TA_AT Cuts at: 160 1032 4276 160 Size: 872 3244 1606 Fragments arranged by size: 3244 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 AvaI C'yCGr_G Cuts at: 1870 1943 2696 3047 1870 Size: 73 753 351 4545 Fragments arranged by size: 4545 753 351 73 BamHI G'GATC_C Cuts at: 3052 3419 3052 Size: 367 5355 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 2634 3043 4498 618 Size: 325 217 627 234 613 409 1455 1842 Fragments arranged by size: 1842 1455 627 613 409 325 234 217 BanII G_rGCy'C Cuts at: 1391 2184 3041 1391 Size: 793 857 4072 Fragments arranged by size: 4072 857 793 BbeI G_GCGC'C Cuts at: 2025 2025 Size: 5722 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4751 BbvCI CC'TCA_GC Cuts at: 2726 2927 2726 Size: 201 5521 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3913 3947 1903 Size: 34 1976 34 3678 Fragments arranged by size: 3678 1976 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3614 4824 3614 Size: 1210 4512 BclI T'GATC_A Cuts at: 1548 2772 1548 Size: 1224 4498 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4333 136 Size: 107 122 71 631 605 2661 1525 Fragments arranged by size: 2661 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2352 5717 2352 Size: 3365 2357 BlpI GC'TnA_GC Cuts at: 3139 3139 Size: 5722 Bme1580I G_kGCm'C Cuts at: 1790 2637 3783 4939 1790 Size: 847 1146 1156 2573 Fragments arranged by size: 2573 1156 1146 847 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5722 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2480 2629 3453 Size: 416 53 83 399 24 1063 149 824 Cuts at: 3453 4411 442 Size: 958 1753 Fragments arranged by size: 1753 1063 958 824 416 399 149 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5722 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 2341 2396 4382 1239 Size: 1088 14 55 1986 2579 Fragments arranged by size: 2579 1986 1088 55 14 Bpu10I CC'TnA_GC Cuts at: 2726 2927 2726 Size: 201 5521 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2168 3847 4712 5010 2168 Size: 1679 865 298 2880 Fragments arranged by size: 2880 1679 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2207 2508 4385 882 Size: 617 708 301 1877 2219 Fragments arranged by size: 2219 1877 708 617 301 BsaAI yAC'GTr Cuts at: 493 1204 3513 493 Size: 711 2309 2702 Fragments arranged by size: 2702 2309 711 BsaBI GATnn'nnATC Cuts at: 2603 5716 2603 Size: 3113 2609 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 2883 3529 3911 Size: 53 83 186 936 489 861 646 382 Cuts at: 3911 4546 275 Size: 635 1451 Fragments arranged by size: 1451 936 861 646 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4155 4553 4827 4974 5385 4155 Size: 398 274 147 411 4492 Fragments arranged by size: 4492 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5497 Fragments arranged by size: 5497 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2393 2414 2423 2441 2444 2537 2540 Size: 943 264 21 9 18 3 93 3 Cuts at: 2540 2543 2609 2944 1186 Size: 3 66 335 3964 Fragments arranged by size: 3964 943 335 264 93 66 21 18 9 3 3 3 BseYI C'CCAG_C Cuts at: 2973 4925 5421 2973 Size: 1952 496 3274 Fragments arranged by size: 3274 1952 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5149 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2041 3172 3933 4961 1540 Size: 275 186 40 1131 761 1028 2301 Fragments arranged by size: 2301 1131 1028 761 275 186 40 BsiHKAI G_wGCw'C Cuts at: 2184 3783 3868 4939 2184 Size: 1599 85 1071 2967 Fragments arranged by size: 2967 1599 1071 85 BsmI GAATG_Cn' Cuts at: 3233 3326 3233 Size: 93 5629 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2184 2637 3041 3783 3868 4939 1391 Size: 399 394 453 404 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 453 404 399 394 85 BspEI T'CCGG_A Cuts at: 5385 5385 Size: 5722 BspHI T'CATG_A Cuts at: 2124 2857 3611 5341 2124 Size: 733 754 1730 2505 Fragments arranged by size: 2505 1730 754 733 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3077 844 Size: 2233 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2254 3609 1394 Size: 429 431 1355 3507 Fragments arranged by size: 3507 1355 431 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4217 4391 65 Size: 1183 2969 174 1396 Fragments arranged by size: 2969 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2036 2637 4366 4553 1700 Size: 110 226 601 1729 187 2869 Fragments arranged by size: 2869 1729 601 226 187 110 BsrGI T'GTAC_A Cuts at: 96 2754 96 Size: 2658 3064 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5722 BssSI C'ACGA_G Cuts at: 2204 3782 4794 2204 Size: 1578 1012 3132 Fragments arranged by size: 3132 1578 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2683 794 Size: 1889 3833 BstBI TT'CG_AA Cuts at: 4526 4526 Size: 5722 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5722 BstXI CCAn_nnnn'nTGG Cuts at: 2197 2197 Size: 5722 BstYI r'GATC_y Cuts at: 1972 2352 2700 3052 3419 3804 3821 5273 5359 Size: 380 348 352 367 385 17 1452 86 Cuts at: 5359 5371 5717 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 380 367 352 348 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2196 513 Size: 552 939 192 4039 Fragments arranged by size: 4039 939 552 192 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2092 487 Size: 683 56 866 4117 Fragments arranged by size: 4117 866 683 56 BtsI GCAGTG_nn' Cuts at: 3238 4032 4052 3238 Size: 794 20 4908 Fragments arranged by size: 4908 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5722 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5687 DraI TTT'AAA Cuts at: 3026 3026 Size: 5722 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2807 4729 809 Size: 507 1491 1922 1802 Fragments arranged by size: 1922 1802 1491 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2038 2268 2415 Size: 54 1425 50 275 18 208 230 147 Cuts at: 2415 3169 4058 5687 8 Size: 754 889 1629 43 Fragments arranged by size: 1629 1425 889 754 275 230 208 147 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2038 3169 1537 Size: 275 226 1131 4090 Fragments arranged by size: 4090 1131 275 226 EarI CTCTTCn'nnn_ Cuts at: 1353 2382 2400 2439 2456 2541 2822 2846 3652 Size: 1029 18 39 17 85 281 24 806 Cuts at: 3652 1353 Size: 3423 Fragments arranged by size: 3423 1029 806 281 85 39 24 18 17 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2656 4309 4683 4829 387 Size: 1064 138 1067 1653 374 146 1280 Fragments arranged by size: 1653 1280 1067 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2182 2182 Size: 5722 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2341 2396 2596 2701 3785 4382 Size: 393 695 14 55 200 105 1084 597 Cuts at: 4382 5169 5437 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1084 787 695 597 393 268 200 105 55 14 EcoNI CCTnn'n_nnAGG Cuts at: 2378 2687 2378 Size: 309 5413 EcoRI G'AATT_C Cuts at: 3031 3409 3031 Size: 378 5344 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2785 2974 2785 Size: 189 5533 FseI GG_CCGG'CC Cuts at: 2040 2040 Size: 5722 FspI TGC'GCA Cuts at: 2157 4228 2157 Size: 2071 3651 HaeII r_GCGC'y Cuts at: 1678 2025 2696 4869 1678 Size: 347 671 2173 2531 Fragments arranged by size: 2531 2173 671 347 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 2714 2746 2764 Size: 32 135 32 599 32 511 32 18 Cuts at: 2764 2796 4363 4395 4437 4469 1373 Size: 32 1567 32 42 32 2626 Fragments arranged by size: 2626 1567 599 511 135 42 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 2491 3066 3312 669 Size: 988 281 553 575 246 3079 Fragments arranged by size: 3079 988 575 553 281 246 HindIII A'AGCT_T Cuts at: 748 2312 2497 748 Size: 1564 185 3973 Fragments arranged by size: 3973 1564 185 HpaI GTT'AAC Cuts at: 3312 3312 Size: 5722 KasI G'GCGC_C Cuts at: 2021 2021 Size: 5722 KpnI G_GTAC'C Cuts at: 3047 3047 Size: 5722 MfeI C'AATT_G Cuts at: 3321 3321 Size: 5722 MluI A'CGCG_T Cuts at: 5598 5598 Size: 5722 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2157 4836 5020 1305 Size: 852 2679 184 2007 Fragments arranged by size: 2679 2007 852 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2270 10 Size: 54 1425 343 438 3462 Fragments arranged by size: 3462 1425 438 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2862 3680 4039 4198 518 Size: 648 1696 818 359 159 2042 Fragments arranged by size: 2042 1696 818 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2266 2973 3138 3815 4963 5208 1606 Size: 660 707 165 677 1148 245 2120 Fragments arranged by size: 2120 1148 707 677 660 245 165 NaeI GCC'GGC Cuts at: 2038 2038 Size: 5722 NarI GG'CG_CC Cuts at: 2022 2022 Size: 5722 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4231 Fragments arranged by size: 4231 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5722 NgoMIV G'CCGG_C Cuts at: 2036 2036 Size: 5722 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5722 NotI GC'GGCC_GC Cuts at: 3169 3169 Size: 5722 NspI r_CATG'y Cuts at: 1132 1229 3080 4625 1132 Size: 97 1851 1545 2229 Fragments arranged by size: 2229 1851 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5722 PciI A'CATG_T Cuts at: 4621 4621 Size: 5722 PflMI CCAn_nnn'nTGG Cuts at: 1108 2973 1108 Size: 1865 3857 PmeI GTTT'AAAC Cuts at: 3026 3026 Size: 5722 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3791 3823 5304 5336 3791 Size: 32 1481 32 4177 Fragments arranged by size: 4177 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5722 PsiI TTA'TAA Cuts at: 3292 3292 Size: 5722 PstI C_TGCA'G Cuts at: 830 2298 2688 3074 830 Size: 1468 390 386 3478 Fragments arranged by size: 3478 1468 390 386 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5722 PvuII CAG'CTG Cuts at: 2266 2266 Size: 5722 SacI G_AGCT'C Cuts at: 2184 2184 Size: 5722 SalI G'TCGA_C Cuts at: 1936 3064 1936 Size: 1128 4594 SapI GCTCTTCn'nnn_ Cuts at: 2541 2541 Size: 5722 SbfI CC_TGCA'GG Cuts at: 3074 3074 Size: 5722 ScaI AGT'ACT Cuts at: 3970 5682 3970 Size: 1712 4010 SfcI C'TryA_G Cuts at: 826 1045 2294 2684 3070 4205 4886 5077 826 Size: 219 1249 390 386 1135 681 191 1471 Fragments arranged by size: 1471 1249 1135 681 390 386 219 191 SfoI GGC'GCC Cuts at: 2023 2023 Size: 5722 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5722 SgrAI Cr'CCGG_yG Cuts at: 1700 2637 1700 Size: 937 4785 SmaI CCC'GGG Cuts at: 1872 3049 1872 Size: 1177 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2183 2696 3826 4727 4989 820 Size: 197 926 240 513 1130 901 262 1553 Fragments arranged by size: 1553 1130 926 901 513 262 240 197 SnaBI TAC'GTA Cuts at: 493 3513 493 Size: 3020 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5722 SphI G_CATG'C Cuts at: 1132 3080 1132 Size: 1948 3774 SspI AAT'ATT Cuts at: 5 52 3646 5 Size: 47 3594 2081 Fragments arranged by size: 3594 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5722 StyI C'CwwG_G Cuts at: 513 1065 2004 2286 2814 3161 513 Size: 552 939 282 528 347 3074 Fragments arranged by size: 3074 939 552 528 347 282 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3749 3766 3919 4104 4549 325 Size: 1255 2169 17 153 185 445 1498 Fragments arranged by size: 2169 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2754 3009 3968 Size: 274 80 33 51 1443 777 255 959 Cuts at: 3968 5680 96 Size: 1712 138 Fragments arranged by size: 1712 1443 959 777 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5682 5714 5682 Size: 32 5690 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5722 XbaI T'CTAG_A Cuts at: 3058 3058 Size: 5722 XhoI C'TCGA_G Cuts at: 1943 2696 1943 Size: 753 4969 XmaI C'CCGG_G Cuts at: 1870 3047 1870 Size: 1177 4545 XmnI GAAnn'nnTTC Cuts at: 2714 3851 2714 Size: 1137 4585 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3530 276 Size: 53 83 186 936 1996 2468 Fragments arranged by size: 2468 1996 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI