Restriction map for
pFN21AE0442
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaI AvaII AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BcgI BciVI BclI BfrI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaBI BsaHI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1407I
BspHI BspLU11I BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cfr10I CpoI DraI DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MaeII MaeIII
MamI MfeI MluI MseI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
SspI StuI StyI SwaI Tru9I
Tsp45I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 708 TCCGGA T^CCGGA
AcsI 398 AAATTT [GA]^AATT[CT]
AfaI 714 GTAC GT^AC
AlwI 183 GATCC GGATC(4/5)
AlwNI 373 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 903 CCTCAGG CC^T[ACGT]AGG
ApoI 398 AAATTT [GA]^AATT[CT]
Asp700I 456 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 790 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 545 TGGCCA TGG^CCA
BbvCI 666 CCTCAGC CCTCAGC(-5/-2)
BcnI 444 CCCGG CC^[GC]GG
BlpI 73 GCTCAGC GC^T[ACGT]AGC
BmyI 246 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 73 GCTCAGC GC^T[ACGT]AGC
BsaI 739 GAGACC GGTCTC(1/5)
BsaWI 708 TCCGGA [AT]^CCGG[AT]
BseAI 708 TCCGGA T^CCGGA
BsmFI 558 GTCCC GGGAC(10/14)
BsmI 237 GAATGC GAATGC(1/-1)
Bsp1286I 246 GTGCCC G[AGT]GC[ACT]^C
BspDI 832 ATCGAT AT^CGAT
BspEI 708 TCCGGA T^CCGGA
BstYI 182 AGATCC [GA]^GATC[CT]
Bsu36I 903 CCTCAGG CC^T[ACGT]AGG
CelII 73 GCTCAGC GC^T[ACGT]AGC
ClaI 832 ATCGAT AT^CGAT
DraIII 799 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 545 TGGCCA [CT]^GGCC[GA]
Eco81I 903 CCTCAGG CC^T[ACGT]AGG
HaeII 115 AGCGCC [GA]GCGC^[CT]
HindIII 230 AAGCTT A^AGCTT
HphI 762 GGTGA GGTGA(8/7)
MflI 182 AGATCC [GA]^GATC[CT]
MluNI 545 TGGCCA TGG^CCA
MroI 708 TCCGGA T^CCGGA
MscI 545 TGGCCA TGG^CCA
MwoI 232 GCTTGGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 444 CCCGG CC^[GC]GG
NspI 506 ACATGC [GA]CATG^[CT]
PflFI 790 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 773 GAGTC GAGTC(4/5)
PshAI 678 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 154 CTGCAG CTGCA^G
RsaI 714 GTAC GT^AC
SapI 304 GCTCTTC GCTCTTC(1/4)
SbfI 153 CCTGCAGG CCTGCA^GG
Sse8387I 153 CCTGCAGG CCTGCA^GG
TspRI 879 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 790 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 182 AGATCC [GA]^GATC[CT]
XmnI 456 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 495 CCGC CCGC(-3/-1)
241 GCGG
BbvI 845 GCTGC GCAGC(8/12)
891 GCTGC
BfaI 295 CTAG C^TAG
514 CTAG
BsaJI 26 CCAGGG C^C[ACGT][ACGT]GG
377 CCCTGG
BsmAI 739 GAGAC GTCTC(1/5)
622 GTCTC
BspMI 8 ACCTGC ACCTGC(4/8)
152 ACCTGC
BsrDI 602 GCAATG GCAATG(2/0)
958 GCAATG
CfoI 116 GCGC GCG^C
170 GCGC
DraII 374 AGGCCCT [GA]G^G[ACGT]CC[CT]
869 AGGCCCC
EcoNI 79 CCTACAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
378 CCTGGCAAAGG
EcoO109I 374 AGGCCCT [GA]G^G[ACGT]CC[CT]
869 AGGCCCC
Fnu4HI 845 GCTGC GC^[ACGT]GC
891 GCTGC
HapII 445 CCGG C^CGG
709 CCGG
HhaI 116 GCGC GCG^C
170 GCGC
HinP1I 116 GCGC G^CGC
170 GCGC
HpaII 445 CCGG C^CGG
709 CCGG
ItaI 845 GCTGC GC^[ACGT]GC
891 GCTGC
MaeI 295 CTAG C^TAG
514 CTAG
MslI 579 CACGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
756 CATGAGGGTG
MspI 445 CCGG C^CGG
709 CCGG
PflMI 354 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
798 CCACCCTGTGG
TseI 845 GCTGC G^C[AT]GC
891 GCTGC
Van91I 354 CCAACACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
798 CCACCCTGTGG
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI BsiYI BslI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII FokI HaeIII HinfI
Ksp632I MboI MboII MnlI MvaI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tsp509I TthHB8I