Restriction map for
pFN21AE0442
(Linear) (Six-base) MAPSORT of: KIEE0442.seq Check: 1054 from: 1 to: 969
KIEE0442.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 292 447 451 949 969
Size: 109 183 155 4 498 20
Fragments arranged by size:
498 183 155 109 20 4
AlwNI CAG_nnn'CTG
Cuts at: 0 378 969
Size: 378 591
ApoI r'AATT_y
Cuts at: 0 398 969
Size: 398 571
BbvCI CC'TCA_GC
Cuts at: 0 667 969
Size: 667 302
BlpI GC'TnA_GC
Cuts at: 0 74 969
Size: 74 895
Bme1580I G_kGCm'C
Cuts at: 0 250 969
Size: 250 719
Bpu10I CC'TnA_GC
Cuts at: 0 598 667 969
Size: 598 69 302
Fragments arranged by size:
598 302 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 733 969
Size: 733 236
BsaWI w'CCGG_w
Cuts at: 0 708 969
Size: 708 261
BsmI GAATG_Cn'
Cuts at: 0 243 969
Size: 243 726
Bsp1286I G_dGCh'C
Cuts at: 0 250 969
Size: 250 719
BspEI T'CCGG_A
Cuts at: 0 708 969
Size: 708 261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 17 161 969
Size: 17 144 808
Fragments arranged by size:
808 144 17
BsrDI GCAATG_nn'
Cuts at: 0 609 965 969
Size: 609 356 4
Fragments arranged by size:
609 356 4
BstYI r'GATC_y
Cuts at: 0 182 969
Size: 182 787
Bsu36I CC'TnA_GG
Cuts at: 0 904 969
Size: 904 65
ClaI AT'CG_AT
Cuts at: 0 833 969
Size: 833 136
DraIII CAC_nnn'GTG
Cuts at: 0 804 969
Size: 804 165
EaeI y'GGCC_r
Cuts at: 0 545 969
Size: 545 424
EarI CTCTTCn'nnn_
Cuts at: 0 311 568 731 969
Size: 311 257 163 238
Fragments arranged by size:
311 257 238 163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 292 447 451 949 969
Size: 109 183 155 4 498 20
Fragments arranged by size:
498 183 155 109 20 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 83 382 969
Size: 83 299 587
Fragments arranged by size:
587 299 83
EcoO109I rG'GnC_Cy
Cuts at: 0 375 870 969
Size: 375 495 99
Fragments arranged by size:
495 375 99
HaeII r_GCGC'y
Cuts at: 0 119 969
Size: 119 850
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 534 566 764 796 969
Size: 534 32 198 32 173
Fragments arranged by size:
534 198 173 32 32
HindIII A'AGCT_T
Cuts at: 0 230 969
Size: 230 739
MscI TGG'CCA
Cuts at: 0 547 969
Size: 547 422
MslI CAynn'nnrTG
Cuts at: 0 583 760 969
Size: 583 177 209
Fragments arranged by size:
583 209 177
NspI r_CATG'y
Cuts at: 0 510 969
Size: 510 459
PflMI CCAn_nnn'nTGG
Cuts at: 0 360 804 969
Size: 360 444 165
Fragments arranged by size:
444 360 165
PfoI T'CCnGG_A
Cuts at: 0 56 969
Size: 56 913
PshAI GACnn'nnGTC
Cuts at: 0 682 969
Size: 682 287
PstI C_TGCA'G
Cuts at: 0 158 969
Size: 158 811
SapI GCTCTTCn'nnn_
Cuts at: 0 311 969
Size: 311 658
SbfI CC_TGCA'GG
Cuts at: 0 158 969
Size: 158 811
SfcI C'TryA_G
Cuts at: 0 80 154 471 969
Size: 80 74 317 498
Fragments arranged by size:
498 317 80 74
TatI w'GTAC_w
Cuts at: 0 713 969
Size: 713 256
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 38 70 969
Size: 38 32 899
Fragments arranged by size:
899 38 32
Tth111I GACn'n_nGTC
Cuts at: 0 793 969
Size: 793 176
XmnI GAAnn'nnTTC
Cuts at: 0 460 969
Size: 460 509
Enzymes that do cut:
AcuI AlwNI ApoI BbvCI BlpI Bme1580I Bpu10I BsaI
BsaWI BsmI Bsp1286I BspEI BspMI BsrDI BstYI Bsu36I
ClaI DraIII EaeI EarI Eco57MI EcoNI EcoO109I HaeII
Hin4I HindIII MscI MslI NspI PflMI PfoI PshAI
PstI SapI SbfI SfcI TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII
BmgBI BmrI BmtI BplI BpmI BpuEI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmBI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI CspCI
DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HpaI KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII XbaI
XcmI XhoI XmaI ZraI