(Circular) (Six-base) MAPSORT of: pFN21AE0043.seq Check: 9076 from: 1 to: 6337 pFN21AE0043 6337 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4147 278 Size: 53 83 186 936 2611 2468 Fragments arranged by size: 2611 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3680 1937 Size: 1743 4594 Acc65I G'GTAC_C Cuts at: 3658 3658 Size: 6337 AclI AA'CG_TT Cuts at: 1563 4464 4837 1563 Size: 2901 373 3063 Fragments arranged by size: 3063 2901 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2723 3390 3430 4400 5784 6052 1632 Size: 1091 667 40 970 1384 268 1917 Fragments arranged by size: 1917 1384 1091 970 667 268 40 AflII C'TTAA_G Cuts at: 820 1017 2998 820 Size: 197 1981 4159 Fragments arranged by size: 4159 1981 197 AflIII A'CryG_T Cuts at: 1203 2133 5236 6213 1203 Size: 930 3103 977 1327 Fragments arranged by size: 3103 1327 977 930 AgeI A'CCGG_T Cuts at: 2819 5168 2819 Size: 2349 3988 AhdI GACnn_n'nnGTC Cuts at: 5066 5066 Size: 6337 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6337 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6337 AlwNI CAG_nnn'CTG Cuts at: 2219 2437 3322 4080 5652 2219 Size: 218 885 758 1572 2904 Fragments arranged by size: 2904 1572 885 758 218 ApaLI G'TGCA_C Cuts at: 3511 4394 5550 3511 Size: 883 1156 4298 Fragments arranged by size: 4298 1156 883 ApoI r'AATT_y Cuts at: 1493 2376 2423 2792 2890 3428 3440 3498 3646 Size: 883 47 369 98 538 12 58 148 Cuts at: 3646 3872 4024 6133 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 883 538 369 226 152 148 98 58 47 12 AseI AT'TA_AT Cuts at: 160 1032 3578 4891 160 Size: 872 2546 1313 1606 Fragments arranged by size: 2546 1606 1313 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6337 AvaI C'yCGr_G Cuts at: 1870 1943 2257 3662 1870 Size: 73 314 1405 4545 Fragments arranged by size: 4545 1405 314 73 BamHI G'GATC_C Cuts at: 3667 4034 3667 Size: 367 5970 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3658 5113 618 Size: 325 217 627 1871 1455 1842 Fragments arranged by size: 1871 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2317 3656 1391 Size: 926 1339 4072 Fragments arranged by size: 4072 1339 926 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2063 2176 2336 2862 3328 928 Size: 971 164 113 160 526 466 3937 Fragments arranged by size: 3937 971 526 466 164 160 113 BbvCI CC'TCA_GC Cuts at: 2609 2609 Size: 6337 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4528 4562 1903 Size: 34 2591 34 3678 Fragments arranged by size: 3678 2591 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4229 5439 4229 Size: 1210 5127 BclI T'GATC_A Cuts at: 1548 3609 1548 Size: 2061 4276 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4948 136 Size: 107 122 71 631 605 3276 1525 Fragments arranged by size: 3276 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2067 3562 6332 2067 Size: 1495 2770 2072 Fragments arranged by size: 2770 2072 1495 BlpI GC'TnA_GC Cuts at: 3754 3754 Size: 6337 Bme1580I G_kGCm'C Cuts at: 1790 2079 2595 2749 3515 4398 5554 1790 Size: 289 516 154 766 883 1156 2573 Fragments arranged by size: 2573 1156 883 766 516 289 154 BmgBI CAC'GTC Cuts at: 1318 2134 1318 Size: 816 5521 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2348 2635 2910 Size: 416 53 83 399 24 931 287 275 Cuts at: 2910 4068 5026 442 Size: 1158 958 1753 Fragments arranged by size: 1753 1158 958 931 416 399 287 275 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 3569 4997 1239 Size: 845 1485 1428 2579 Fragments arranged by size: 2579 1485 1428 845 Bpu10I CC'TnA_GC Cuts at: 2609 2609 Size: 6337 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4462 5327 5625 4462 Size: 865 298 5174 Fragments arranged by size: 5174 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5000 882 Size: 617 3501 2219 Fragments arranged by size: 3501 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4128 493 Size: 711 2924 2702 Fragments arranged by size: 2924 2702 711 BsaBI GATnn'nnATC Cuts at: 6331 6331 Size: 6337 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4144 4526 5161 275 Size: 53 83 186 936 2611 382 635 1451 Fragments arranged by size: 2611 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2819 4770 5168 5442 5589 6000 2819 Size: 1951 398 274 147 411 3156 Fragments arranged by size: 3156 1951 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2925 2955 1178 Size: 30 165 30 1522 30 4560 Fragments arranged by size: 4560 1522 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6337 BseYI C'CCAG_C Cuts at: 2445 5540 6036 2445 Size: 3095 496 2746 Fragments arranged by size: 3095 2746 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5764 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3787 4548 5576 1540 Size: 275 186 1786 761 1028 2301 Fragments arranged by size: 2301 1786 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3515 4398 4483 5554 3515 Size: 883 85 1071 4298 Fragments arranged by size: 4298 1071 883 85 BsmI GAATG_Cn' Cuts at: 3848 3941 3848 Size: 93 6244 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2317 2595 2749 3515 3656 4398 Size: 399 289 238 278 154 766 141 742 Cuts at: 4398 4483 5554 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 766 742 399 289 278 238 154 141 85 BspEI T'CCGG_A Cuts at: 6000 6000 Size: 6337 BspHI T'CATG_A Cuts at: 4226 5956 4226 Size: 1730 4607 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3692 844 Size: 2848 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4224 1394 Size: 429 2401 3507 Fragments arranged by size: 3507 2401 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4832 5006 65 Size: 1183 3584 174 1396 Fragments arranged by size: 3584 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2819 4981 5168 1700 Size: 110 1009 2162 187 2869 Fragments arranged by size: 2869 2162 1009 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6337 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6337 BssSI C'ACGA_G Cuts at: 2588 4397 5409 2588 Size: 1809 1012 3516 Fragments arranged by size: 3516 1809 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6337 BstBI TT'CG_AA Cuts at: 5141 5141 Size: 6337 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6337 BstXI CCAn_nnnn'nTGG Cuts at: 2305 2305 Size: 6337 BstYI r'GATC_y Cuts at: 1972 2067 2496 2664 3562 3667 4034 4419 4436 Size: 95 429 168 898 105 367 385 17 Cuts at: 4436 5888 5974 5986 6332 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 898 429 385 367 346 168 105 95 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2324 2324 Size: 6337 BtgI C'CryG_G Cuts at: 513 1065 2004 2298 513 Size: 552 939 294 4552 Fragments arranged by size: 4552 939 552 294 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5598 Fragments arranged by size: 5598 683 56 BtsI GCAGTG_nn' Cuts at: 2622 3853 4647 4667 2622 Size: 1231 794 20 4292 Fragments arranged by size: 4292 1231 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6337 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6302 DraI TTT'AAA Cuts at: 3641 3641 Size: 6337 DraIII CAC_nnn'GTG Cuts at: 3164 3164 Size: 6337 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5344 809 Size: 507 4028 1802 Fragments arranged by size: 4028 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3784 4673 6302 Size: 54 1425 50 275 18 1954 889 1629 Cuts at: 6302 8 Size: 43 Fragments arranged by size: 1954 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3784 1537 Size: 275 1972 4090 Fragments arranged by size: 4090 1972 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2239 2570 3471 4267 1353 Size: 886 331 901 796 3423 Fragments arranged by size: 3423 901 886 796 331 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4924 5298 5444 387 Size: 1064 138 3335 374 146 1280 Fragments arranged by size: 3335 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2723 3390 3430 3569 4400 4997 Size: 393 452 639 667 40 139 831 597 Cuts at: 4997 5784 6052 1239 Size: 787 268 1524 Fragments arranged by size: 1524 831 787 667 639 597 452 393 268 139 40 EcoO109I rG'GnC_Cy Cuts at: 2254 3319 2254 Size: 1065 5272 EcoRI G'AATT_C Cuts at: 2376 3440 3646 4024 2376 Size: 1064 206 378 4689 Fragments arranged by size: 4689 1064 378 206 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2368 2368 Size: 6337 FspI TGC'GCA Cuts at: 4843 4843 Size: 6337 HaeII r_GCGC'y Cuts at: 1678 2124 5484 1678 Size: 446 3360 2531 Fragments arranged by size: 3360 2531 446 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2216 2248 3158 3190 4978 Size: 32 135 32 644 32 910 32 1788 Cuts at: 4978 5010 5052 5084 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1788 910 644 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2560 3681 3927 669 Size: 988 281 622 1121 246 3079 Fragments arranged by size: 3079 1121 988 622 281 246 HindIII A'AGCT_T Cuts at: 748 2012 2802 3035 748 Size: 1264 790 233 4050 Fragments arranged by size: 4050 1264 790 233 HpaI GTT'AAC Cuts at: 3927 3927 Size: 6337 KpnI G_GTAC'C Cuts at: 3662 3662 Size: 6337 MfeI C'AATT_G Cuts at: 3936 3936 Size: 6337 MluI A'CGCG_T Cuts at: 6213 6213 Size: 6337 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2700 5451 5635 1305 Size: 1395 2751 184 2007 Fragments arranged by size: 2751 2007 1395 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4515 Fragments arranged by size: 4515 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2303 2366 4295 4654 4813 518 Size: 648 1137 63 1929 359 159 2042 Fragments arranged by size: 2042 1929 1137 648 359 159 63 MspA1I CmG'CkG Cuts at: 1606 2219 2661 3753 4430 5578 5823 1606 Size: 613 442 1092 677 1148 245 2120 Fragments arranged by size: 2120 1148 1092 677 613 442 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2298 513 Size: 552 939 294 4552 Fragments arranged by size: 4552 939 552 294 NdeI CA'TA_TG Cuts at: 387 387 Size: 6337 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6337 NotI GC'GGCC_GC Cuts at: 3784 3784 Size: 6337 NspI r_CATG'y Cuts at: 1132 1229 3695 5240 1132 Size: 97 2466 1545 2229 Fragments arranged by size: 2466 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6337 PciI A'CATG_T Cuts at: 5236 5236 Size: 6337 PflMI CCAn_nnn'nTGG Cuts at: 1108 2437 1108 Size: 1329 5008 PfoI T'CCnGG_A Cuts at: 2943 2943 Size: 6337 PmeI GTTT'AAAC Cuts at: 3641 3641 Size: 6337 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4406 4438 5919 5951 4406 Size: 32 1481 32 4792 Fragments arranged by size: 4792 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2254 2254 Size: 6337 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6337 PsiI TTA'TAA Cuts at: 3907 3907 Size: 6337 PstI C_TGCA'G Cuts at: 830 2224 3689 830 Size: 1394 1465 3478 Fragments arranged by size: 3478 1465 1394 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6337 PvuII CAG'CTG Cuts at: 2219 2661 2219 Size: 442 5895 SalI G'TCGA_C Cuts at: 1936 3679 1936 Size: 1743 4594 SapI GCTCTTCn'nnn_ Cuts at: 3471 3471 Size: 6337 SbfI CC_TGCA'GG Cuts at: 3689 3689 Size: 6337 ScaI AGT'ACT Cuts at: 4585 6297 4585 Size: 1712 4625 SexAI A'CCwGG_T Cuts at: 2250 2404 2436 2250 Size: 154 32 6151 Fragments arranged by size: 6151 154 32 SfcI C'TryA_G Cuts at: 826 1045 2220 3685 4820 5501 5692 826 Size: 219 1175 1465 1135 681 191 1471 Fragments arranged by size: 1471 1465 1175 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6337 SgrAI Cr'CCGG_yG Cuts at: 1700 2819 1700 Size: 1119 5218 SmaI CCC'GGG Cuts at: 1872 2259 3664 1872 Size: 387 1405 4545 Fragments arranged by size: 4545 1405 387 SmlI C'TyrA_G Cuts at: 820 1017 1943 2998 4441 5342 5604 820 Size: 197 926 1055 1443 901 262 1553 Fragments arranged by size: 1553 1443 1055 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4128 493 Size: 3635 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6337 SphI G_CATG'C Cuts at: 1132 3695 1132 Size: 2563 3774 SspI AAT'ATT Cuts at: 5 52 3058 3582 4261 5 Size: 47 3006 524 679 2081 Fragments arranged by size: 3006 2081 679 524 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6337 StyI C'CwwG_G Cuts at: 513 1065 2004 2298 2575 3776 513 Size: 552 939 294 277 1201 3074 Fragments arranged by size: 3074 1201 939 552 294 277 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2325 2617 2929 2996 4364 4381 4534 Size: 1255 745 292 312 67 1368 17 153 Cuts at: 4534 4719 5164 325 Size: 185 445 1498 Fragments arranged by size: 1498 1368 1255 745 445 312 292 185 153 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2140 2335 4583 Size: 274 80 33 51 1443 163 195 2248 Cuts at: 4583 6295 96 Size: 1712 138 Fragments arranged by size: 2248 1712 1443 274 195 163 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2286 2318 2615 2647 6297 6329 2286 Size: 32 297 32 3650 32 2294 Fragments arranged by size: 3650 2294 297 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2252 3347 1316 Size: 936 1095 4306 Fragments arranged by size: 4306 1095 936 XbaI T'CTAG_A Cuts at: 3673 3673 Size: 6337 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2501 2501 Size: 6337 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6337 XmaI C'CCGG_G Cuts at: 1870 2257 3662 1870 Size: 387 1405 4545 Fragments arranged by size: 4545 1405 387 XmnI GAAnn'nnTTC Cuts at: 2013 3480 4466 2013 Size: 1467 986 3884 Fragments arranged by size: 3884 1467 986 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4145 276 Size: 53 83 186 936 2611 2468 Fragments arranged by size: 2611 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI