Restriction map for
pFN21AB6310
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI Alw44I AlwNI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AviII AvrII BanI BbeI
BbrPI BbsI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeIII MamI MfeI MluI MroI
MseI MunI NaeI NarI NdeI
NgoMIV NheI NotI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp45I
Tsp509I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AhdI 208 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 670 CCTGAGG CC^T[ACGT]AGG
Aor51HI 490 AGCGCT AGC^GCT
ApaI 746 GGGCCC GGGCC^C
AspEI 208 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 121 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 248 TGGCCA TGG^CCA
BamHI 162 GGATCC G^GATCC
BbvCI 391 GCTGAGG CCTCAGC(-5/-2)
BcgI 459 CGAGCAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 20 GAGACC GGTCTC(1/5)
BsmAI 20 GAGAC GTCTC(1/5)
BsrI 596 CCAGT ACTGG(1/-1)
BstXI 305 CCACGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 670 CCTGAGG CC^T[ACGT]AGG
CfoI 491 GCGC GCG^C
Eam1105I 208 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 511 GAAGAG CTCTTC(1/4)
Eco47III 490 AGCGCT AGC^GCT
Eco81I 670 CCTGAGG CC^T[ACGT]AGG
EcoNI 810 CCTCTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 490 AGCGCT [GA]GCGC^[CT]
HhaI 491 GCGC GCG^C
HinP1I 491 GCGC G^CGC
HinfI 208 GACTC G^A[ACGT]TC
HphI 667 TCACC GGTGA(8/7)
Ksp632I 511 GAAGAG CTCTTC(1/4)
MaeII 236 ACGT A^CGT
MboII 511 GAAGA GAAGA(8/7)
MluNI 248 TGGCCA TGG^CCA
MscI 248 TGGCCA TGG^CCA
MslI 306 CACGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NruI 16 TCGCGA TCG^CGA
PflFI 121 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 558 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 208 GACTC GAGTC(4/5)
PshAI 655 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
StuI 626 AGGCCT AGG^CCT
TaqI 341 TCGA T^CGA
Tth111I 121 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 341 TCGA T^CGA
Van91I 558 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccII 17 CGCG CG^CG
325 CGCG
AlwI 163 GATCC GGATC(4/5)
162 GGATC
BglI 361 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
536 GCCCCCAGGGC
BglII 84 AGATCT A^GATCT
561 AGATCT
BsgI 520 CTGCAC GTGCAG(16/14)
274 GTGCAG
BstUI 17 CGCG CG^CG
325 CGCG
EaeI 248 TGGCCA [CT]^GGCC[GA]
308 CGGCCA
MvnI 17 CGCG CG^CG
325 CGCG
NcoI 311 CCATGG C^CATGG
794 CCATGG
PstI 446 CTGCAG CTGCA^G
721 CTGCAG
PvuII 389 CAGCTG CAG^CTG
657 CAGCTG
SfcI 446 CTGCAG C^T[GA][CT]AG
721 CTGCAG
SmaI 742 CCCGGG CCC^GGG
777 CCCGGG
TspRI 485 CACTG CA[GC]TG[ACGT][ACGT]^
597 CAGTG
XmaCI 742 CCCGGG C^CCGGG
777 CCCGGG
XmaI 742 CCCGGG C^CCGGG
777 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AvaI AvaII BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI BsoBI
Bsp1286I BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HpaII
ItaI MboI MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI Sau3AI Sau96I
ScrFI SfaNI StyI TseI XhoII