(Circular) (Six-base) MAPSORT of: pFN21AB6310.seq Check: 654 from: 1 to: 5545 pFN21AB6310 5545 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3355 278 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2888 1937 Size: 951 4594 Acc65I G'GTAC_C Cuts at: 2866 2866 Size: 5545 AclI AA'CG_TT Cuts at: 1563 2844 3672 4045 1563 Size: 1281 828 373 3063 Fragments arranged by size: 3063 1281 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2570 2612 3608 4992 5260 1632 Size: 938 42 996 1384 268 1917 Fragments arranged by size: 1917 1384 996 938 268 42 AfeI AGC'GCT Cuts at: 2497 2497 Size: 5545 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5348 AflIII A'CryG_T Cuts at: 1203 4444 5421 1203 Size: 3241 977 1327 Fragments arranged by size: 3241 1327 977 AgeI A'CCGG_T Cuts at: 4376 4376 Size: 5545 AhdI GACnn_n'nnGTC Cuts at: 2218 4274 2218 Size: 2056 3489 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5545 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5545 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2819 2851 2819 Size: 32 5513 AlwNI CAG_nnn'CTG Cuts at: 3288 4860 3288 Size: 1572 3973 ApaI G_GGCC'C Cuts at: 2755 2755 Size: 5545 ApaLI G'TGCA_C Cuts at: 3602 4758 3602 Size: 1156 4389 ApoI r'AATT_y Cuts at: 1493 2854 3080 3232 5341 1493 Size: 1361 226 152 2109 1697 Fragments arranged by size: 2109 1697 1361 226 152 AseI AT'TA_AT Cuts at: 160 1032 4099 160 Size: 872 3067 1606 Fragments arranged by size: 3067 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 AvaI C'yCGr_G Cuts at: 1870 1943 2098 2322 2531 2747 2762 2782 2870 Size: 73 155 224 209 216 15 20 88 Cuts at: 2870 1870 Size: 4545 Fragments arranged by size: 4545 224 216 209 155 88 73 20 15 BamHI G'GATC_C Cuts at: 2167 2875 3242 2167 Size: 708 367 4470 Fragments arranged by size: 4470 708 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2866 4321 618 Size: 325 217 627 1079 1455 1842 Fragments arranged by size: 1842 1455 1079 627 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2685 2755 2789 2864 1391 Size: 621 673 70 34 75 4072 Fragments arranged by size: 4072 673 621 75 70 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4574 BbvCI CC'TCA_GC Cuts at: 2397 2397 Size: 5545 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2453 2487 3736 3770 1903 Size: 34 516 34 1249 34 3678 Fragments arranged by size: 3678 1249 516 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3437 4647 3437 Size: 1210 4335 BclI T'GATC_A Cuts at: 1548 1548 Size: 5545 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2372 2547 4156 Size: 107 122 71 631 605 700 175 1609 Cuts at: 4156 136 Size: 1525 Fragments arranged by size: 1609 1525 700 631 605 175 122 107 71 BglII A'GATC_T Cuts at: 2089 2566 5540 2089 Size: 477 2974 2094 Fragments arranged by size: 2974 2094 477 BlpI GC'TnA_GC Cuts at: 2962 2962 Size: 5545 Bme1580I G_kGCm'C Cuts at: 1790 2044 2368 2755 3606 4762 1790 Size: 254 324 387 851 1156 2573 Fragments arranged by size: 2573 1156 851 387 324 254 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5545 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2595 3276 4234 Size: 416 53 83 399 24 1178 681 958 Cuts at: 4234 442 Size: 1753 Fragments arranged by size: 1753 1178 958 681 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5545 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2106 2200 2380 2591 4205 1239 Size: 867 94 180 211 1614 2579 Fragments arranged by size: 2579 1614 867 211 180 94 Bpu10I CC'TnA_GC Cuts at: 2397 2397 Size: 5545 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3670 4535 4833 3670 Size: 865 298 4382 Fragments arranged by size: 4382 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2019 4208 882 Size: 617 520 2189 2219 Fragments arranged by size: 2219 2189 617 520 BsaAI yAC'GTr Cuts at: 493 1204 3336 493 Size: 711 2132 2702 Fragments arranged by size: 2702 2132 711 BsaBI GATnn'nnATC Cuts at: 5539 5539 Size: 5545 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3352 3734 4369 275 Size: 53 83 186 936 1819 382 635 1451 Fragments arranged by size: 1819 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3978 4376 4650 4797 5208 3978 Size: 398 274 147 411 4315 Fragments arranged by size: 4315 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2104 2134 2795 2825 1178 Size: 30 165 30 701 30 661 30 3898 Fragments arranged by size: 3898 701 661 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2534 2629 2693 2837 1186 Size: 1348 95 64 144 3894 Fragments arranged by size: 3894 1348 144 95 64 BseYI C'CCAG_C Cuts at: 2367 2379 2392 2645 2687 2714 4748 5244 2367 Size: 12 13 253 42 27 2034 496 2668 Fragments arranged by size: 2668 2034 496 253 42 27 13 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 2510 1163 Size: 573 564 210 4198 Fragments arranged by size: 4198 573 564 210 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2995 3756 4784 1540 Size: 275 186 994 761 1028 2301 Fragments arranged by size: 2301 1028 994 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3606 3691 4762 3606 Size: 85 1071 4389 Fragments arranged by size: 4389 1071 85 BsmI GAATG_Cn' Cuts at: 3056 3149 3056 Size: 93 5452 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2044 2368 2685 2755 2789 2864 Size: 399 222 32 324 317 70 34 75 Cuts at: 2864 3606 3691 4762 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 324 317 222 85 75 70 34 32 BspEI T'CCGG_A Cuts at: 5208 5208 Size: 5545 BspHI T'CATG_A Cuts at: 3434 5164 3434 Size: 1730 3815 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2900 844 Size: 2056 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3432 1394 Size: 429 1609 3507 Fragments arranged by size: 3507 1609 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4040 4214 65 Size: 1183 2792 174 1396 Fragments arranged by size: 2792 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4189 4376 1700 Size: 110 2379 187 2869 Fragments arranged by size: 2869 2379 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5545 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5545 BssSI C'ACGA_G Cuts at: 3605 4617 3605 Size: 1012 4533 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5545 BstBI TT'CG_AA Cuts at: 4349 4349 Size: 5545 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5545 BstXI CCAn_nnnn'nTGG Cuts at: 2317 2317 Size: 5545 BstYI r'GATC_y Cuts at: 1972 2089 2167 2566 2875 3242 3627 3644 5096 Size: 117 78 399 309 367 385 17 1452 Cuts at: 5096 5182 5194 5540 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 399 385 367 346 309 117 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 2676 2676 Size: 5545 BtgI C'CryG_G Cuts at: 513 1065 2004 2115 2310 2316 2799 513 Size: 552 939 111 195 6 483 3259 Fragments arranged by size: 3259 939 552 483 195 111 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4806 Fragments arranged by size: 4806 683 56 BtsI GCAGTG_nn' Cuts at: 3061 3855 3875 3061 Size: 794 20 4731 Fragments arranged by size: 4731 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5545 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5510 DraI TTT'AAA Cuts at: 2849 2849 Size: 5545 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4552 809 Size: 507 3236 1802 Fragments arranged by size: 3236 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 2313 2992 Size: 54 1425 50 275 18 423 60 679 Cuts at: 2992 3881 5510 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 679 423 275 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2992 1537 Size: 275 1180 4090 Fragments arranged by size: 4090 1180 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2511 3475 1353 Size: 1158 964 3423 Fragments arranged by size: 3423 1158 964 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4132 4506 4652 387 Size: 1064 138 2543 374 146 1280 Fragments arranged by size: 2543 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2106 2200 2380 2570 2591 2612 3608 Size: 393 474 94 180 190 21 21 996 Cuts at: 3608 4205 4992 5260 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 996 787 597 474 393 268 190 180 94 21 21 EcoNI CCTnn'n_nnAGG Cuts at: 2819 2819 Size: 5545 EcoO109I rG'GnC_Cy Cuts at: 2220 2751 2759 2772 2220 Size: 531 8 13 4993 Fragments arranged by size: 4993 531 13 8 EcoRI G'AATT_C Cuts at: 2854 3232 2854 Size: 378 5167 FspI TGC'GCA Cuts at: 4051 4051 Size: 5545 HaeII r_GCGC'y Cuts at: 1678 2499 4692 1678 Size: 821 2193 2531 Fragments arranged by size: 2531 2193 821 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2081 2113 2204 2236 2795 Size: 32 135 32 509 32 91 32 559 Cuts at: 2795 2827 4186 4218 4260 4292 1373 Size: 32 1359 32 42 32 2626 Fragments arranged by size: 2626 1359 559 509 135 91 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2889 3135 669 Size: 988 281 951 246 3079 Fragments arranged by size: 3079 988 951 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5545 HpaI GTT'AAC Cuts at: 3135 3135 Size: 5545 KpnI G_GTAC'C Cuts at: 2870 2870 Size: 5545 MfeI C'AATT_G Cuts at: 3144 3144 Size: 5545 MluI A'CGCG_T Cuts at: 5421 5421 Size: 5545 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2235 4659 4843 1305 Size: 930 2424 184 2007 Fragments arranged by size: 2424 2007 930 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2255 10 Size: 54 1425 343 423 3300 Fragments arranged by size: 3300 1425 423 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2315 3503 3862 4021 518 Size: 648 1149 1188 359 159 2042 Fragments arranged by size: 2042 1188 1149 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2371 2396 2664 2696 2808 2961 3638 4786 Size: 765 25 268 32 112 153 677 1148 Cuts at: 4786 5031 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 765 677 268 245 153 112 32 25 NcoI C'CATG_G Cuts at: 513 1065 2004 2316 2799 513 Size: 552 939 312 483 3259 Fragments arranged by size: 3259 939 552 483 312 NdeI CA'TA_TG Cuts at: 387 387 Size: 5545 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5545 NotI GC'GGCC_GC Cuts at: 2992 2992 Size: 5545 NruI TCG'CGA Cuts at: 2023 2023 Size: 5545 NspI r_CATG'y Cuts at: 1132 1229 2903 4448 1132 Size: 97 1674 1545 2229 Fragments arranged by size: 2229 1674 1545 97 PasI CC'CwG_GG Cuts at: 1348 2065 2545 1348 Size: 717 480 4348 Fragments arranged by size: 4348 717 480 PciI A'CATG_T Cuts at: 4444 4444 Size: 5545 PflMI CCAn_nnn'nTGG Cuts at: 1108 2569 1108 Size: 1461 4084 PfoI T'CCnGG_A Cuts at: 2421 2421 Size: 5545 PmeI GTTT'AAAC Cuts at: 2849 2849 Size: 5545 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3614 3646 5127 5159 3614 Size: 32 1481 32 4000 Fragments arranged by size: 4000 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2220 2759 2772 2220 Size: 539 13 4993 Fragments arranged by size: 4993 539 13 PshAI GACnn'nnGTC Cuts at: 1613 2664 1613 Size: 1051 4494 PsiI TTA'TAA Cuts at: 3115 3115 Size: 5545 PspOMI G'GGCC_C Cuts at: 2751 2751 Size: 5545 PstI C_TGCA'G Cuts at: 830 2455 2730 2897 830 Size: 1625 275 167 3478 Fragments arranged by size: 3478 1625 275 167 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5545 PvuII CAG'CTG Cuts at: 2396 2664 2396 Size: 268 5277 SalI G'TCGA_C Cuts at: 1936 2887 1936 Size: 951 4594 SanDI GG'GwC_CC Cuts at: 2772 2772 Size: 5545 SbfI CC_TGCA'GG Cuts at: 2897 2897 Size: 5545 ScaI AGT'ACT Cuts at: 3793 5505 3793 Size: 1712 3833 SfcI C'TryA_G Cuts at: 826 1045 2451 2726 2893 4028 4709 4900 826 Size: 219 1406 275 167 1135 681 191 1471 Fragments arranged by size: 1471 1406 1135 681 275 219 191 167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5545 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5545 SmaI CCC'GGG Cuts at: 1872 2749 2784 2872 1872 Size: 877 35 88 4545 Fragments arranged by size: 4545 877 88 35 SmlI C'TyrA_G Cuts at: 820 1017 1943 3649 4550 4812 820 Size: 197 926 1706 901 262 1553 Fragments arranged by size: 1706 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3336 493 Size: 2843 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5545 SphI G_CATG'C Cuts at: 1132 2903 1132 Size: 1771 3774 SspI AAT'ATT Cuts at: 5 52 3469 5 Size: 47 3417 2081 Fragments arranged by size: 3417 2081 47 StuI AGG'CCT Cuts at: 1517 2633 1517 Size: 1116 4429 StyI C'CwwG_G Cuts at: 513 1065 2004 2171 2316 2755 2799 2984 513 Size: 552 939 167 145 439 44 185 3074 Fragments arranged by size: 3074 939 552 439 185 167 145 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3572 3589 3742 3927 4372 325 Size: 1255 1992 17 153 185 445 1498 Fragments arranged by size: 1992 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3791 5503 96 Size: 274 80 33 51 1443 1814 1712 138 Fragments arranged by size: 1814 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2104 2136 5505 5537 2104 Size: 32 3369 32 2112 Fragments arranged by size: 3369 2112 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2129 1316 Size: 813 4732 XbaI T'CTAG_A Cuts at: 2881 2881 Size: 5545 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5545 XmaI C'CCGG_G Cuts at: 1870 2747 2782 2870 1870 Size: 877 35 88 4545 Fragments arranged by size: 4545 877 88 35 XmnI GAAnn'nnTTC Cuts at: 3674 3674 Size: 5545 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3353 276 Size: 53 83 186 936 1819 2468 Fragments arranged by size: 2468 1819 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI