Restriction map for
pFN21AB7677
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# NO CUT ENZYMES ( 139 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII BalI BbeI BbvCI
BcgI BciVI BclI BfrI BglI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HincII
HindII HpaI KasI KpnI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AatII 310 GACGTC GACGT^C
AflIII 686 ACGTGT A^C[GA][CT]GT
AlwNI 786 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 367 GGGCCC GGGCC^C
AvrII 57 CCTAGG C^CTAGG
BamHI 768 GGATCC G^GATCC
BbrPI 67 CACGTG CAC^GTG
BfaI 58 CTAG C^TAG
BglII 752 AGATCT A^GATCT
BlnI 57 CCTAGG C^CTAGG
BseRI 559 GAGGAG GAGGAG(10/8)
BsmAI 837 GTCTC GTCTC(1/5)
BssSI 839 CTCGTG CACGAG(-5/-1)
BstAPI 180 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI 224 CCGCGG C^C[GA][CT]GG
EaeI 513 CGGCCA [CT]^GGCC[GA]
EarI 430 GAAGAG CTCTTC(1/4)
HindIII 862 AAGCTT A^AGCTT
Ksp632I 430 GAAGAG CTCTTC(1/4)
KspI 224 CCGCGG CCGC^GG
MaeI 58 CTAG C^TAG
PflMI 785 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 67 CACGTG CAC^GTG
PmlI 67 CACGTG CAC^GTG
PpuMI 601 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 694 CAGCTG CAG^CTG
SacI 589 GAGCTC GAGCT^C
SacII 224 CCGCGG CCGC^GG
ScaI 206 AGTACT AGT^ACT
TaqI 239 TCGA T^CGA
TthHB8I 239 TCGA T^CGA
Van91I 785 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AfaI 207 GTAC GT^AC
333 GTAC
AspHI 229 GAGCAC G[AT]GC[AT]^C
589 GAGCTC
BanI 61 GGCACC G^G[CT][GA]CC
287 GGCACC
BsaAI 67 CACGTG [CT]AC^GT[GA]
685 TACGTG
BsgI 178 CTGCAC GTGCAG(16/14)
584 GTGCAG
BsiHKAI 229 GAGCAC G[AT]GC[AT]^C
589 GAGCTC
BsmFI 421 GTCCC GGGAC(10/14)
564 GTCCC
BspMI 98 ACCTGC ACCTGC(4/8)
473 ACCTGC
DdeI 303 CTCAG C^T[ACGT]AG
449 CTCAG
EcoNI 52 CCTCCCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
267 CCTCACCAAGG
EcoT14I 57 CCTAGG C^C[AT][AT]GG
272 CCAAGG
FauI 151 CCCGC CCCGC(4/6)
625 CCCGC
MslI 63 CACCCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
517 CACCTGGATG
PstI 478 CTGCAG CTGCA^G
597 CTGCAG
RsaI 207 GTAC GT^AC
333 GTAC
SfcI 478 CTGCAG C^T[GA][CT]AG
597 CTGCAG
StyI 57 CCTAGG C^C[AT][AT]GG
272 CCAAGG
Tsp509I 14 AATT ^AATT
93 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaII BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp45I TspRI XhoII