(Circular) (Six-base) MAPSORT of: pFN21AB7677.seq Check: 7477 from: 1 to: 5728 pFN21AB7677 5728 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2487 2487 Size: 5728 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2319 3538 278 Size: 53 83 186 936 783 1219 2468 Fragments arranged by size: 2468 1219 936 783 186 83 53 AccI GT'mk_AC Cuts at: 1937 3071 1937 Size: 1134 4594 Acc65I G'GTAC_C Cuts at: 3049 3049 Size: 5728 AclI AA'CG_TT Cuts at: 1563 3855 4228 1563 Size: 2292 373 3063 Fragments arranged by size: 3063 2292 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2377 2420 3791 5175 5443 1632 Size: 745 43 1371 1384 268 1917 Fragments arranged by size: 1917 1384 1371 745 268 43 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5531 AflIII A'CryG_T Cuts at: 1203 2691 4627 5604 1203 Size: 1488 1936 977 1327 Fragments arranged by size: 1936 1488 1327 977 AgeI A'CCGG_T Cuts at: 4559 4559 Size: 5728 AhdI GACnn_n'nnGTC Cuts at: 4457 4457 Size: 5728 AleI CACnn'nnGTG Cuts at: 1166 2072 1166 Size: 906 4822 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2090 1184 Size: 906 4822 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2225 2257 2225 Size: 32 5696 AlwNI CAG_nnn'CTG Cuts at: 2796 3471 5043 2796 Size: 675 1572 3481 Fragments arranged by size: 3481 1572 675 ApaI G_GGCC'C Cuts at: 2376 2376 Size: 5728 ApaLI G'TGCA_C Cuts at: 3785 4941 3785 Size: 1156 4572 ApoI r'AATT_y Cuts at: 1493 3037 3263 3415 5524 1493 Size: 1544 226 152 2109 1697 Fragments arranged by size: 2109 1697 1544 226 152 AseI AT'TA_AT Cuts at: 160 1032 4282 160 Size: 872 3250 1606 Fragments arranged by size: 3250 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 AvaI C'yCGr_G Cuts at: 1870 1943 3053 1870 Size: 73 1110 4545 Fragments arranged by size: 4545 1110 73 AvrII C'CTAG_G Cuts at: 2062 2062 Size: 5728 BamHI G'GATC_C Cuts at: 2773 3058 3425 2773 Size: 285 367 5076 Fragments arranged by size: 5076 367 285 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2292 3049 4504 618 Size: 325 217 627 279 226 757 1455 1842 Fragments arranged by size: 1842 1455 757 627 325 279 226 217 BanII G_rGCy'C Cuts at: 1391 2362 2376 2598 2727 2787 3047 1391 Size: 971 14 222 129 60 260 4072 Fragments arranged by size: 4072 971 260 222 129 60 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2146 2408 2748 928 Size: 971 247 262 340 3908 Fragments arranged by size: 3908 971 340 262 247 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3919 3953 1903 Size: 34 50 34 1898 34 3678 Fragments arranged by size: 3678 1898 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3620 4830 3620 Size: 1210 4518 BclI T'GATC_A Cuts at: 1548 1548 Size: 5728 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4339 136 Size: 107 122 71 631 605 2667 1525 Fragments arranged by size: 2667 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2757 5723 2757 Size: 2966 2762 BlpI GC'TnA_GC Cuts at: 3145 3145 Size: 5728 Bme1580I G_kGCm'C Cuts at: 1790 2376 2851 3789 4945 1790 Size: 586 475 938 1156 2573 Fragments arranged by size: 2573 1156 938 586 475 BmgBI CAC'GTC Cuts at: 1318 2179 1318 Size: 861 4867 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3459 4417 442 Size: 416 53 83 399 24 2042 958 1753 Fragments arranged by size: 2042 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5728 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2318 2406 2981 2990 4388 1239 Size: 1079 88 575 9 1398 2579 Fragments arranged by size: 2579 1398 1079 575 88 9 Bpu10I CC'TnA_GC Cuts at: 2308 2308 Size: 5728 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3853 4718 5016 3853 Size: 865 298 4565 Fragments arranged by size: 4565 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4391 882 Size: 617 2892 2219 Fragments arranged by size: 2892 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2074 2692 3519 493 Size: 711 870 618 827 2702 Fragments arranged by size: 2702 870 827 711 618 BsaBI GATnn'nnATC Cuts at: 5722 5722 Size: 5728 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2316 2405 2751 3535 Size: 53 83 186 936 783 89 346 784 Cuts at: 3535 3917 4552 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 784 783 635 382 346 186 89 83 53 BsaWI w'CCGG_w Cuts at: 3025 4161 4559 4833 4980 5391 3025 Size: 1136 398 274 147 411 3362 Fragments arranged by size: 3362 1136 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2225 2255 1178 Size: 30 165 30 822 30 4651 Fragments arranged by size: 4651 822 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2579 1186 Size: 1393 4335 BseYI C'CCAG_C Cuts at: 2107 2196 2303 2349 2412 4931 5427 2107 Size: 89 107 46 63 2519 496 2408 Fragments arranged by size: 2519 2408 496 107 89 63 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2168 2610 1163 Size: 573 432 442 4281 Fragments arranged by size: 4281 573 442 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3178 3939 4967 1540 Size: 275 186 1177 761 1028 2301 Fragments arranged by size: 2301 1177 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2238 2598 3789 3874 4945 2238 Size: 360 1191 85 1071 3021 Fragments arranged by size: 3021 1191 1071 360 85 BsmI GAATG_Cn' Cuts at: 3239 3332 3239 Size: 93 5635 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2238 2362 2376 2598 2727 2787 2851 Size: 399 448 124 14 222 129 60 64 Cuts at: 2851 3047 3789 3874 4945 1391 Size: 196 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 448 399 222 196 129 124 85 64 60 14 BspEI T'CCGG_A Cuts at: 5391 5391 Size: 5728 BspHI T'CATG_A Cuts at: 3617 5347 3617 Size: 1730 3998 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2112 2487 3083 844 Size: 1268 375 596 3489 Fragments arranged by size: 3489 1268 596 375 BsrBI CCG'CTC Cuts at: 1394 1823 3615 1394 Size: 429 1792 3507 Fragments arranged by size: 3507 1792 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4223 4397 65 Size: 1183 2975 174 1396 Fragments arranged by size: 2975 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4372 4559 1700 Size: 110 2562 187 2869 Fragments arranged by size: 2869 2562 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5728 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5728 BssSI C'ACGA_G Cuts at: 2844 3788 4800 2844 Size: 944 1012 3772 Fragments arranged by size: 3772 1012 944 BstAPI GCAn_nnn'nTGC Cuts at: 794 2191 794 Size: 1397 4331 BstBI TT'CG_AA Cuts at: 4532 4532 Size: 5728 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5728 BstYI r'GATC_y Cuts at: 1972 2121 2388 2757 2773 3058 3425 3810 3827 Size: 149 267 369 16 285 367 385 17 Cuts at: 3827 5279 5365 5377 5723 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 369 367 346 285 267 149 86 17 16 12 BtgI C'CryG_G Cuts at: 513 1065 2229 513 Size: 552 1164 4012 Fragments arranged by size: 4012 1164 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2648 487 Size: 683 56 1422 3567 Fragments arranged by size: 3567 1422 683 56 BtsI GCAGTG_nn' Cuts at: 3244 4038 4058 3244 Size: 794 20 4914 Fragments arranged by size: 4914 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5728 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5693 DraI TTT'AAA Cuts at: 3032 3032 Size: 5728 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4735 809 Size: 507 3419 1802 Fragments arranged by size: 3419 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2518 3175 4064 Size: 54 1425 50 275 18 688 657 889 Cuts at: 4064 5693 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 688 657 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3175 1537 Size: 275 1363 4090 Fragments arranged by size: 4090 1363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2430 3658 1353 Size: 1077 1228 3423 Fragments arranged by size: 3423 1228 1077 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4315 4689 4835 387 Size: 1064 138 2726 374 146 1280 Fragments arranged by size: 2726 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2596 2596 Size: 5728 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2318 2377 2406 2420 2981 2990 3791 Size: 393 686 59 29 14 561 9 801 Cuts at: 3791 4388 5175 5443 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 801 787 686 597 561 393 268 59 29 14 9 EcoNI CCTnn'n_nnAGG Cuts at: 2061 2276 2061 Size: 215 5513 EcoO109I rG'GnC_Cy Cuts at: 2373 2607 2644 2373 Size: 234 37 5457 Fragments arranged by size: 5457 234 37 EcoRI G'AATT_C Cuts at: 3037 3415 3037 Size: 378 5350 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2302 2302 Size: 5728 FspI TGC'GCA Cuts at: 4234 4234 Size: 5728 HaeII r_GCGC'y Cuts at: 1678 4875 1678 Size: 3197 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2133 2165 2247 2279 2825 Size: 32 135 32 561 32 82 32 546 Cuts at: 2825 2857 4369 4401 4443 4475 1373 Size: 32 1512 32 42 32 2626 Fragments arranged by size: 2626 1512 561 546 135 82 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3072 3318 669 Size: 988 281 1134 246 3079 Fragments arranged by size: 3079 1134 988 281 246 HindIII A'AGCT_T Cuts at: 748 2867 748 Size: 2119 3609 HpaI GTT'AAC Cuts at: 3318 3318 Size: 5728 KpnI G_GTAC'C Cuts at: 3053 3053 Size: 5728 MfeI C'AATT_G Cuts at: 3327 3327 Size: 5728 MluI A'CGCG_T Cuts at: 5604 5604 Size: 5728 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4842 5026 1305 Size: 3537 184 2007 Fragments arranged by size: 3537 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3906 Fragments arranged by size: 3906 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2072 2526 3686 4045 4204 518 Size: 648 906 454 1160 359 159 2042 Fragments arranged by size: 2042 1160 906 648 454 359 159 MspA1I CmG'CkG Cuts at: 1606 2231 2353 2701 2968 3144 3821 4969 5214 Size: 625 122 348 267 176 677 1148 245 Cuts at: 5214 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 625 348 267 245 176 122 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5176 NdeI CA'TA_TG Cuts at: 387 387 Size: 5728 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5728 NotI GC'GGCC_GC Cuts at: 3175 3175 Size: 5728 NspI r_CATG'y Cuts at: 1132 1229 3086 4631 1132 Size: 97 1857 1545 2229 Fragments arranged by size: 2229 1857 1545 97 PasI CC'CwG_GG Cuts at: 1348 2498 2611 1348 Size: 1150 113 4465 Fragments arranged by size: 4465 1150 113 PciI A'CATG_T Cuts at: 4627 4627 Size: 5728 PflMI CCAn_nnn'nTGG Cuts at: 1108 2796 1108 Size: 1688 4040 PfoI T'CCnGG_A Cuts at: 2926 2940 2926 Size: 14 5714 PmeI GTTT'AAAC Cuts at: 3032 3032 Size: 5728 PmlI CAC'GTG Cuts at: 2074 2074 Size: 5728 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2225 2257 3797 3829 5310 5342 2225 Size: 32 1540 32 1481 32 2611 Fragments arranged by size: 2611 1540 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2607 2607 Size: 5728 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5728 PsiI TTA'TAA Cuts at: 2865 3298 2865 Size: 433 5295 PspOMI G'GGCC_C Cuts at: 2372 2372 Size: 5728 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2813 2845 2813 Size: 32 5696 PstI C_TGCA'G Cuts at: 830 2487 2606 3080 830 Size: 1657 119 474 3478 Fragments arranged by size: 3478 1657 474 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5728 PvuII CAG'CTG Cuts at: 2701 2701 Size: 5728 SacI G_AGCT'C Cuts at: 2598 2598 Size: 5728 SacII CC_GC'GG Cuts at: 2232 2232 Size: 5728 SalI G'TCGA_C Cuts at: 1936 3070 1936 Size: 1134 4594 SbfI CC_TGCA'GG Cuts at: 3080 3080 Size: 5728 ScaI AGT'ACT Cuts at: 2213 3976 5688 2213 Size: 1763 1712 2253 Fragments arranged by size: 2253 1763 1712 SfcI C'TryA_G Cuts at: 826 1045 2483 2602 3076 4211 4892 5083 826 Size: 219 1438 119 474 1135 681 191 1471 Fragments arranged by size: 1471 1438 1135 681 474 219 191 119 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5728 SmaI CCC'GGG Cuts at: 1872 3055 1872 Size: 1183 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3832 4733 4995 820 Size: 197 926 1889 901 262 1553 Fragments arranged by size: 1889 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3519 493 Size: 3026 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5728 SphI G_CATG'C Cuts at: 1132 3086 1132 Size: 1954 3774 SspI AAT'ATT Cuts at: 5 52 3652 5 Size: 47 3600 2081 Fragments arranged by size: 3600 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5728 StyI C'CwwG_G Cuts at: 513 1065 2062 2277 3167 513 Size: 552 997 215 890 3074 Fragments arranged by size: 3074 997 890 552 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2084 2099 2426 3755 3772 3925 4110 Size: 1255 504 15 327 1329 17 153 185 Cuts at: 4110 4555 325 Size: 445 1498 Fragments arranged by size: 1498 1329 1255 504 445 327 185 153 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2211 2337 3974 Size: 274 80 33 51 1443 234 126 1637 Cuts at: 3974 5686 96 Size: 1712 138 Fragments arranged by size: 1712 1637 1443 274 234 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5688 5720 5688 Size: 32 5696 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5728 XbaI T'CTAG_A Cuts at: 3064 3064 Size: 5728 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5728 XmaI C'CCGG_G Cuts at: 1870 3053 1870 Size: 1183 4545 XmnI GAAnn'nnTTC Cuts at: 3857 3857 Size: 5728 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2317 3536 276 Size: 53 83 186 936 783 1219 2468 Fragments arranged by size: 2468 1219 936 783 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI