Restriction map for
pFN21AB7677
(Linear) (Six-base) MAPSORT of: KIBB7677.seq Check: 9204 from: 1 to: 1023
KIBB7677.orf
With 182 enzymes: SgfI *
October 16, 2009 16:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 482 1023
Size: 482 541
AatII G_ACGT'C
Cuts at: 0 314 1023
Size: 314 709
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 415 1023
Size: 372 43 608
Fragments arranged by size:
608 372 43
AflIII A'CryG_T
Cuts at: 0 686 1023
Size: 686 337
AleI CACnn'nnGTG
Cuts at: 0 67 1023
Size: 67 956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 85 1023
Size: 85 938
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 220 252 1023
Size: 220 32 771
Fragments arranged by size:
771 220 32
AlwNI CAG_nnn'CTG
Cuts at: 0 791 1023
Size: 791 232
ApaI G_GGCC'C
Cuts at: 0 371 1023
Size: 371 652
AvrII C'CTAG_G
Cuts at: 0 57 1023
Size: 57 966
BamHI G'GATC_C
Cuts at: 0 768 1023
Size: 768 255
BanI G'GyrC_C
Cuts at: 0 61 287 1023
Size: 61 226 736
Fragments arranged by size:
736 226 61
BanII G_rGCy'C
Cuts at: 0 357 371 593 722 782 1023
Size: 357 14 222 129 60 241
Fragments arranged by size:
357 241 222 129 60 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 141 403 743 1023
Size: 141 262 340 280
Fragments arranged by size:
340 280 262 141
BglII A'GATC_T
Cuts at: 0 752 1023
Size: 752 271
Bme1580I G_kGCm'C
Cuts at: 0 371 846 1023
Size: 371 475 177
Fragments arranged by size:
475 371 177
BmgBI CAC'GTC
Cuts at: 0 174 1023
Size: 174 849
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 401 976 985 1023
Size: 313 88 575 9 38
Fragments arranged by size:
575 313 88 38 9
Bpu10I CC'TnA_GC
Cuts at: 0 303 1023
Size: 303 720
BsaAI yAC'GTr
Cuts at: 0 69 687 1023
Size: 69 618 336
Fragments arranged by size:
618 336 69
BsaHI Gr'CG_yC
Cuts at: 0 311 400 746 1023
Size: 311 89 346 277
Fragments arranged by size:
346 311 277 89
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 1023
Size: 220 30 773
Fragments arranged by size:
773 220 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 574 1023
Size: 574 449
BseYI C'CCAG_C
Cuts at: 0 102 191 298 344 407 1023
Size: 102 89 107 46 63 616
Fragments arranged by size:
616 107 102 89 63 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 605 1023
Size: 163 442 418
Fragments arranged by size:
442 418 163
BsiHKAI G_wGCw'C
Cuts at: 0 233 593 1023
Size: 233 360 430
Fragments arranged by size:
430 360 233
Bsp1286I G_dGCh'C
Cuts at: 0 233 357 371 593 722 782 846 1023
Size: 233 124 14 222 129 60 64 177
Fragments arranged by size:
233 222 177 129 124 64 60 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 482 1023
Size: 107 375 541
Fragments arranged by size:
541 375 107
BssSI C'ACGA_G
Cuts at: 0 839 1023
Size: 839 184
BstAPI GCAn_nnn'nTGC
Cuts at: 0 186 1023
Size: 186 837
BstYI r'GATC_y
Cuts at: 0 116 383 752 768 1023
Size: 116 267 369 16 255
Fragments arranged by size:
369 267 255 116 16
BtgI C'CryG_G
Cuts at: 0 224 1023
Size: 224 799
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 643 1023
Size: 643 380
EaeI y'GGCC_r
Cuts at: 0 513 1023
Size: 513 510
EarI CTCTTCn'nnn_
Cuts at: 0 425 1023
Size: 425 598
EcoICRI GAG'CTC
Cuts at: 0 591 1023
Size: 591 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 372 401 415 976 985 1023
Size: 313 59 29 14 561 9 38
Fragments arranged by size:
561 313 59 38 29 14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 56 271 1023
Size: 56 215 752
Fragments arranged by size:
752 215 56
EcoO109I rG'GnC_Cy
Cuts at: 0 368 602 639 1023
Size: 368 234 37 384
Fragments arranged by size:
384 368 234 37
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 297 1023
Size: 297 726
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 128 160 242 274 820 852 1023
Size: 128 32 82 32 546 32 171
Fragments arranged by size:
546 171 128 82 32 32 32
HindIII A'AGCT_T
Cuts at: 0 862 1023
Size: 862 161
MslI CAynn'nnrTG
Cuts at: 0 67 521 1023
Size: 67 454 502
Fragments arranged by size:
502 454 67
MspA1I CmG'CkG
Cuts at: 0 226 348 696 963 1023
Size: 226 122 348 267 60
Fragments arranged by size:
348 267 226 122 60
PasI CC'CwG_GG
Cuts at: 0 493 606 1023
Size: 493 113 417
Fragments arranged by size:
493 417 113
PflMI CCAn_nnn'nTGG
Cuts at: 0 791 1023
Size: 791 232
PfoI T'CCnGG_A
Cuts at: 0 921 935 1023
Size: 921 14 88
Fragments arranged by size:
921 88 14
PmlI CAC'GTG
Cuts at: 0 69 1023
Size: 69 954
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 1023
Size: 220 32 771
Fragments arranged by size:
771 220 32
PpuMI rG'GwC_Cy
Cuts at: 0 602 1023
Size: 602 421
PsiI TTA'TAA
Cuts at: 0 860 1023
Size: 860 163
PspOMI G'GGCC_C
Cuts at: 0 367 1023
Size: 367 656
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 808 840 1023
Size: 808 32 183
Fragments arranged by size:
808 183 32
PstI C_TGCA'G
Cuts at: 0 482 601 1023
Size: 482 119 422
Fragments arranged by size:
482 422 119
PvuII CAG'CTG
Cuts at: 0 696 1023
Size: 696 327
SacI G_AGCT'C
Cuts at: 0 593 1023
Size: 593 430
SacII CC_GC'GG
Cuts at: 0 227 1023
Size: 227 796
ScaI AGT'ACT
Cuts at: 0 208 1023
Size: 208 815
SfcI C'TryA_G
Cuts at: 0 478 597 1023
Size: 478 119 426
Fragments arranged by size:
478 426 119
StyI C'CwwG_G
Cuts at: 0 57 272 1023
Size: 57 215 751
Fragments arranged by size:
751 215 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 94 421 1023
Size: 79 15 327 602
Fragments arranged by size:
602 327 79 15
TatI w'GTAC_w
Cuts at: 0 206 332 1023
Size: 206 126 691
Fragments arranged by size:
691 206 126
ZraI GAC'GTC
Cuts at: 0 312 1023
Size: 312 711
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AlfI AloI AlwNI
ApaI AvrII BamHI BanI BanII BbsI BglII Bme1580I
BmgBI BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BssSI BstAPI BstYI BtgI
BtgZI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
Hin4I HindIII MslI MspA1I PasI PflMI PfoI PmlI
PpiI PpuMI PsiI PspOMI PsrI PstI PvuII SacI
SacII ScaI SfcI StyI TaqII TatI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI ApaLI
ApoI AscI AseI AsiSI AvaI BaeI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BlpI BmrI BmtI
BplI BpuEI BsaI BsaBI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PshAI PspXI PvuI RsrII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI