(Circular) (Six-base) MAPSORT of: pFN21AB5951.seq Check: 4491 from: 1 to: 6235 pFN21AB5951 6235 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4045 278 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3336 3578 1937 Size: 1399 242 4594 Fragments arranged by size: 4594 1399 242 Acc65I G'GTAC_C Cuts at: 3556 3556 Size: 6235 AclI AA'CG_TT Cuts at: 1563 4362 4735 1563 Size: 2799 373 3063 Fragments arranged by size: 3063 2799 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2154 2202 3092 3425 4298 5682 5950 1632 Size: 522 48 890 333 873 1384 268 1917 Fragments arranged by size: 1917 1384 890 873 522 333 268 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6038 AflIII A'CryG_T Cuts at: 1203 5134 6111 1203 Size: 3931 977 1327 Fragments arranged by size: 3931 1327 977 AgeI A'CCGG_T Cuts at: 5066 5066 Size: 6235 AhdI GACnn_n'nnGTC Cuts at: 4964 4964 Size: 6235 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6235 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2511 2592 1184 Size: 1327 81 4827 Fragments arranged by size: 4827 1327 81 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2196 2228 2742 2774 2811 2843 2196 Size: 32 514 32 37 32 5588 Fragments arranged by size: 5588 514 37 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2555 2605 3124 3978 5550 2555 Size: 50 519 854 1572 3240 Fragments arranged by size: 3240 1572 854 519 50 ApaI G_GGCC'C Cuts at: 2525 2847 3084 2525 Size: 322 237 5676 Fragments arranged by size: 5676 322 237 ApaLI G'TGCA_C Cuts at: 4292 5448 4292 Size: 1156 5079 ApoI r'AATT_y Cuts at: 1493 3341 3544 3770 3922 6031 1493 Size: 1848 203 226 152 2109 1697 Fragments arranged by size: 2109 1848 1697 226 203 152 AseI AT'TA_AT Cuts at: 160 1032 4789 160 Size: 872 3757 1606 Fragments arranged by size: 3757 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 AvaI C'yCGr_G Cuts at: 1870 1943 2830 2892 3560 1870 Size: 73 887 62 668 4545 Fragments arranged by size: 4545 887 668 73 62 BamHI G'GATC_C Cuts at: 3565 3932 3565 Size: 367 5868 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3556 5011 618 Size: 325 217 627 1769 1455 1842 Fragments arranged by size: 1842 1769 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2525 2847 3084 3554 1391 Size: 1134 322 237 470 4072 Fragments arranged by size: 4072 1134 470 322 237 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3471 928 Size: 971 1572 3692 Fragments arranged by size: 3692 1572 971 BbvCI CC'TCA_GC Cuts at: 2697 3428 2697 Size: 731 5504 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2873 2907 3509 3543 4426 4460 1903 Size: 34 936 34 602 34 883 34 3678 Fragments arranged by size: 3678 936 883 602 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2649 4127 5337 2649 Size: 1478 1210 3547 Fragments arranged by size: 3547 1478 1210 BclI T'GATC_A Cuts at: 1548 2020 1548 Size: 472 5763 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4846 136 Size: 107 122 71 631 605 3174 1525 Fragments arranged by size: 3174 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2116 6230 2116 Size: 4114 2121 BlpI GC'TnA_GC Cuts at: 3269 3652 3269 Size: 383 5852 Bme1580I G_kGCm'C Cuts at: 1790 2525 2847 3084 4296 5452 1790 Size: 735 322 237 1212 1156 2573 Fragments arranged by size: 2573 1212 1156 735 322 237 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6235 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2501 2721 3966 Size: 416 53 83 399 24 1084 220 1245 Cuts at: 3966 4924 442 Size: 958 1753 Fragments arranged by size: 1753 1245 1084 958 416 399 220 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6235 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3294 3294 Size: 6235 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2733 2944 3342 4895 1239 Size: 1494 211 398 1553 2579 Fragments arranged by size: 2579 1553 1494 398 211 Bpu10I CC'TnA_GC Cuts at: 2697 3428 2697 Size: 731 5504 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4360 5225 5523 4360 Size: 865 298 5072 Fragments arranged by size: 5072 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4898 882 Size: 617 3399 2219 Fragments arranged by size: 3399 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4026 493 Size: 711 2822 2702 Fragments arranged by size: 2822 2702 711 BsaBI GATnn'nnATC Cuts at: 6229 6229 Size: 6235 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4042 4424 5059 275 Size: 53 83 186 936 2509 382 635 1451 Fragments arranged by size: 2509 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3205 4668 5066 5340 5487 5898 3205 Size: 1463 398 274 147 411 3542 Fragments arranged by size: 3542 1463 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2273 2303 1178 Size: 30 165 30 870 30 5110 Fragments arranged by size: 5110 870 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2281 2685 3449 1186 Size: 1095 404 764 3972 Fragments arranged by size: 3972 1095 764 404 BseYI C'CCAG_C Cuts at: 2848 3145 3225 5438 5934 2848 Size: 297 80 2213 496 3149 Fragments arranged by size: 3149 2213 496 297 80 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2264 1163 Size: 573 528 5134 Fragments arranged by size: 5134 573 528 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3685 4446 5474 1540 Size: 275 186 1684 761 1028 2301 Fragments arranged by size: 2301 1684 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4296 4381 5452 4296 Size: 85 1071 5079 Fragments arranged by size: 5079 1071 85 BsmI GAATG_Cn' Cuts at: 2072 3746 3839 2072 Size: 1674 93 4468 Fragments arranged by size: 4468 1674 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2525 2847 3084 3554 4296 4381 5452 Size: 399 735 322 237 470 742 85 1071 Cuts at: 5452 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 735 470 399 322 237 85 BspEI T'CCGG_A Cuts at: 5898 5898 Size: 6235 BspHI T'CATG_A Cuts at: 4124 5854 4124 Size: 1730 4505 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3590 844 Size: 2746 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3276 4122 1394 Size: 429 1453 846 3507 Fragments arranged by size: 3507 1453 846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3162 4730 4904 65 Size: 1183 1914 1568 174 1396 Fragments arranged by size: 1914 1568 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3243 4879 5066 1700 Size: 110 1433 1636 187 2869 Fragments arranged by size: 2869 1636 1433 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6235 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6235 BssSI C'ACGA_G Cuts at: 4295 5307 4295 Size: 1012 5223 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6235 BstBI TT'CG_AA Cuts at: 5039 5039 Size: 6235 BstEII G'GTnAC_C Cuts at: 1184 3127 1184 Size: 1943 4292 BstYI r'GATC_y Cuts at: 1972 2116 2149 2992 3565 3932 4317 4334 5786 Size: 144 33 843 573 367 385 17 1452 Cuts at: 5786 5872 5884 6230 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 843 573 385 367 346 144 86 33 17 12 Bsu36I CC'TnA_GG Cuts at: 2463 2482 2781 2463 Size: 19 299 5917 Fragments arranged by size: 5917 299 19 BtgI C'CryG_G Cuts at: 513 1065 2004 3372 513 Size: 552 939 1368 3376 Fragments arranged by size: 3376 1368 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5496 Fragments arranged by size: 5496 683 56 BtsI GCAGTG_nn' Cuts at: 3751 4545 4565 3751 Size: 794 20 5421 Fragments arranged by size: 5421 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6200 DraI TTT'AAA Cuts at: 3539 3539 Size: 6235 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5242 809 Size: 507 3926 1802 Fragments arranged by size: 3926 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3245 3682 4571 Size: 54 1425 50 275 18 1415 437 889 Cuts at: 4571 6200 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1415 889 437 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3682 1537 Size: 275 1870 4090 Fragments arranged by size: 4090 1870 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2130 3204 3371 4165 1353 Size: 777 1074 167 794 3423 Fragments arranged by size: 3423 1074 794 777 167 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4822 5196 5342 387 Size: 1064 138 3233 374 146 1280 Fragments arranged by size: 3233 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2202 2733 2944 3092 3342 3425 Size: 393 522 48 531 211 148 250 83 Cuts at: 3425 4298 4895 5682 5950 1239 Size: 873 597 787 268 1524 Fragments arranged by size: 1524 873 787 597 531 522 393 268 250 211 148 83 48 EcoNI CCTnn'n_nnAGG Cuts at: 2374 2700 3431 2374 Size: 326 731 5178 Fragments arranged by size: 5178 731 326 EcoO109I rG'GnC_Cy Cuts at: 2522 2827 2843 2844 3080 3081 2522 Size: 305 16 1 236 1 5676 Fragments arranged by size: 5676 305 236 16 1 1 EcoRI G'AATT_C Cuts at: 3544 3922 3544 Size: 378 5857 FspI TGC'GCA Cuts at: 4741 4741 Size: 6235 HaeII r_GCGC'y Cuts at: 1678 3226 5382 1678 Size: 1548 2156 2531 Fragments arranged by size: 2531 2156 1548 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2210 2242 2288 2320 4876 Size: 32 135 32 638 32 46 32 2556 Cuts at: 4876 4908 4950 4982 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2556 638 135 46 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2159 3579 3825 669 Size: 988 281 221 1420 246 3079 Fragments arranged by size: 3079 1420 988 281 246 221 HindIII A'AGCT_T Cuts at: 748 748 Size: 6235 HpaI GTT'AAC Cuts at: 3825 3825 Size: 6235 KpnI G_GTAC'C Cuts at: 3560 3560 Size: 6235 MfeI C'AATT_G Cuts at: 3834 3834 Size: 6235 MluI A'CGCG_T Cuts at: 6111 6111 Size: 6235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2282 5349 5533 1305 Size: 977 3067 184 2007 Fragments arranged by size: 3067 2007 977 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4413 Fragments arranged by size: 4413 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4193 4552 4711 518 Size: 648 3027 359 159 2042 Fragments arranged by size: 3027 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2501 3112 3451 3498 3651 4328 5476 5721 Size: 895 611 339 47 153 677 1148 245 Cuts at: 5721 1606 Size: 2120 Fragments arranged by size: 2120 1148 895 677 611 339 245 153 47 NcoI C'CATG_G Cuts at: 513 1065 2004 3372 513 Size: 552 939 1368 3376 Fragments arranged by size: 3376 1368 939 552 NdeI CA'TA_TG Cuts at: 387 2983 387 Size: 2596 3639 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6235 NotI GC'GGCC_GC Cuts at: 3682 3682 Size: 6235 NspI r_CATG'y Cuts at: 1132 1229 2492 2839 3593 5138 1132 Size: 97 1263 347 754 1545 2229 Fragments arranged by size: 2229 1545 1263 754 347 97 PasI CC'CwG_GG Cuts at: 1348 2045 1348 Size: 697 5538 PciI A'CATG_T Cuts at: 5134 5134 Size: 6235 PflMI CCAn_nnn'nTGG Cuts at: 1108 2555 1108 Size: 1447 4788 PfoI T'CCnGG_A Cuts at: 2710 3381 3483 2710 Size: 671 102 5462 Fragments arranged by size: 5462 671 102 PmeI GTTT'AAAC Cuts at: 3539 3539 Size: 6235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3085 3117 4304 4336 5817 5849 3085 Size: 32 1187 32 1481 32 3471 Fragments arranged by size: 3471 1481 1187 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2827 2827 Size: 6235 PshAI GACnn'nnGTC Cuts at: 1613 2668 1613 Size: 1055 5180 PsiI TTA'TAA Cuts at: 3805 3805 Size: 6235 PspOMI G'GGCC_C Cuts at: 2521 2843 3080 2521 Size: 322 237 5676 Fragments arranged by size: 5676 322 237 PstI C_TGCA'G Cuts at: 830 2500 2610 2767 3503 3587 830 Size: 1670 110 157 736 84 3478 Fragments arranged by size: 3478 1670 736 157 110 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6235 PvuII CAG'CTG Cuts at: 2501 3112 2501 Size: 611 5624 SalI G'TCGA_C Cuts at: 1936 3577 1936 Size: 1641 4594 SanDI GG'GwC_CC Cuts at: 2827 2827 Size: 6235 SapI GCTCTTCn'nnn_ Cuts at: 2130 2130 Size: 6235 SbfI CC_TGCA'GG Cuts at: 3587 3587 Size: 6235 ScaI AGT'ACT Cuts at: 4483 6195 4483 Size: 1712 4523 SexAI A'CCwGG_T Cuts at: 2235 2235 Size: 6235 SfcI C'TryA_G Cuts at: 826 1045 2496 2606 2763 3499 3583 4718 5399 Size: 219 1451 110 157 736 84 1135 681 Cuts at: 5399 5590 826 Size: 191 1471 Fragments arranged by size: 1471 1451 1135 736 681 219 191 157 110 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6235 SmaI CCC'GGG Cuts at: 1872 2832 3562 1872 Size: 960 730 4545 Fragments arranged by size: 4545 960 730 SmlI C'TyrA_G Cuts at: 820 1017 1943 4339 5240 5502 820 Size: 197 926 2396 901 262 1553 Fragments arranged by size: 2396 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4026 493 Size: 3533 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6235 SphI G_CATG'C Cuts at: 1132 2492 2839 3593 1132 Size: 1360 347 754 3774 Fragments arranged by size: 3774 1360 754 347 SspI AAT'ATT Cuts at: 5 52 4159 5 Size: 47 4107 2081 Fragments arranged by size: 4107 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6235 StyI C'CwwG_G Cuts at: 513 1065 2004 3372 3674 513 Size: 552 939 1368 302 3074 Fragments arranged by size: 3074 1368 939 552 302 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2446 2450 4262 4279 4432 4617 5062 Size: 1255 866 4 1812 17 153 185 445 Cuts at: 5062 325 Size: 1498 Fragments arranged by size: 1812 1498 1255 866 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4481 6193 96 Size: 274 80 33 51 1443 2504 1712 138 Fragments arranged by size: 2504 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2273 2305 2844 2876 6195 6227 2273 Size: 32 539 32 3319 32 2281 Fragments arranged by size: 3319 2281 539 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6235 XbaI T'CTAG_A Cuts at: 3571 3571 Size: 6235 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6235 XmaI C'CCGG_G Cuts at: 1870 2830 3560 1870 Size: 960 730 4545 Fragments arranged by size: 4545 960 730 XmnI GAAnn'nnTTC Cuts at: 4364 4364 Size: 6235 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4043 276 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI XcmI