Restriction map for
pFN21AB7738
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I AspEI
AspHI AvrII BamHI BbeI BbrPI
BbvCI BcgI BfrI BglI BlnI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
Hin1I HincII HindII HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TfiI XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 85 CGCG CG^CG
AflIII 1247 ACGTGT A^C[GA][CT]GT
AocI 1120 CCTGAGG CC^T[ACGT]AGG
Aor51HI 90 AGCGCT AGC^GCT
ApaI 859 GGGCCC GGGCC^C
AspI 1354 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 823 TGCGCA TGC^GCA
BalI 519 TGGCCA TGG^CCA
BbsI 550 GTCTTC GAAGAC(2/6)
BciVI 22 GGATAC GTATCC(6/5)
BclI 773 TGATCA T^GATCA
BfaI 256 CTAG C^TAG
BglII 153 AGATCT A^GATCT
BlpI 1182 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1182 GCTGAGC GC^T[ACGT]AGC
BpuAI 550 GTCTTC GAAGAC(2/6)
BsaAI 1246 TACGTG [CT]AC^GT[GA]
BsmBI 184 GAGACG CGTCTC(1/5)
Bsp1407I 1250 TGTACA T^GTACA
BsrDI 1077 CATTGC GCAATG(2/0)
BsrGI 1250 TGTACA T^GTACA
BsrI 380 CCAGT ACTGG(1/-1)
BstUI 85 CGCG CG^CG
BstXI 1419 CCAGCCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1120 CCTGAGG CC^T[ACGT]AGG
CelII 1182 GCTGAGC GC^T[ACGT]AGC
DraIII 816 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 90 AGCGCT AGC^GCT
Eco81I 1120 CCTGAGG CC^T[ACGT]AGG
EcoNI 326 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 773 TGATCA T^GATCA
FspI 823 TGCGCA TGC^GCA
HgaI 1308 GCGTC GACGC(5/10)
MaeI 256 CTAG C^TAG
MluNI 519 TGGCCA TGG^CCA
MscI 519 TGGCCA TGG^CCA
MseI 674 TTAA T^TAA
MslI 1372 CACTTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 85 CGCG CG^CG
PflFI 1354 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1133 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmlI 1318 CTCAAG C^T[CT][GA]AG
SspBI 1250 TGTACA T^GTACA
Tru9I 674 TTAA T^TAA
Tth111I 1354 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1133 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 480 GAATTT [GA]^AATT[CT]
946 AAATTT
AlwNI 559 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1134 CAGGCTCTG
ApoI 480 GAATTT [GA]^AATT[CT]
946 AAATTT
BpmI 378 CTCCAG CTGGAG(16/14)
866 CTCCAG
BseRI 1123 GAGGAG GAGGAG(10/8)
1348 GAGGAG
BsmAI 184 GAGAC GTCTC(1/5)
956 GAGAC
BsrBI 1188 CCGCTC CCGCTC(-3/-3)
4 GAGCGG
BsrFI 1157 ACCGGC [GA]^CCGG[CT]
1334 ACCGGC
Cfr10I 1157 ACCGGC [GA]^CCGG[CT]
1334 ACCGGC
DraII 859 GGGCCCT [GA]G^G[ACGT]CC[CT]
1274 AGGCCCT
DrdI 805 GACTCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1333 GACCGGCCTGTC
EaeI 519 TGGCCA [CT]^GGCC[GA]
1159 CGGCCA
EarI 157 CTCTTC CTCTTC(1/4)
1279 CTCTTC
EcoO109I 859 GGGCCCT [GA]G^G[ACGT]CC[CT]
1274 AGGCCCT
HaeII 90 AGCGCT [GA]GCGC^[CT]
1007 AGCGCC
Ksp632I 157 CTCTTC CTCTTC(1/4)
1279 CTCTTC
MaeII 187 ACGT A^CGT
1247 ACGT
PstI 327 CTGCAG CTGCA^G
1021 CTGCAG
PvuII 634 CAGCTG CAG^CTG
1024 CAGCTG
SmaI 123 CCCGGG CCC^GGG
464 CCCGGG
TaqI 143 TCGA T^CGA
987 TCGA
TthHB8I 143 TCGA T^CGA
987 TCGA
XcmI 507 CCAGCCCCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
513 CCACCCTGGCCATGG
XmaCI 123 CCCGGG C^CCGGG
464 CCCGGG
XmaI 123 CCCGGG C^CCGGG
464 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
Acc65I AciI AfaI AluI AlwI
Asp718I AvaI AvaII BanI BanII
BbvI BcnI BmyI BsaJI BsiYI
BslI BsmFI BsoBI Bsp1286I BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI KpnI MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I Tsp509I TspRI XhoII