Restriction map for
pFN21AB7738
(Circular) (Six-base) MAPSORT of: pFN21AB7738.seq Check: 9287 from: 1 to: 6157
pFN21AB7738 6157 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3967 278
Size: 53 83 186 936 2431 2468
Fragments arranged by size:
2468 2431 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3500 1937
Size: 1563 4594
Acc65I G'GTAC_C
Cuts at: 2212 2532 2757 3478 2212
Size: 320 225 721 4891
Fragments arranged by size:
4891 721 320 225
AclI AA'CG_TT
Cuts at: 1563 4284 4657 1563
Size: 2721 373 3063
Fragments arranged by size:
3063 2721 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2041 2175 2223 4220 5604 5872 1632
Size: 409 134 48 1997 1384 268 1917
Fragments arranged by size:
1997 1917 1384 409 268 134 48
AfeI AGC'GCT
Cuts at: 2097 2097
Size: 6157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5960
AflIII A'CryG_T
Cuts at: 1203 3252 5056 6033 1203
Size: 2049 1804 977 1327
Fragments arranged by size:
2049 1804 1327 977
AgeI A'CCGG_T
Cuts at: 4988 4988
Size: 6157
AhdI GACnn_n'nnGTC
Cuts at: 4886 4886
Size: 6157
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6157
AlwNI CAG_nnn'CTG
Cuts at: 2569 3144 3900 5472 2569
Size: 575 756 1572 3254
Fragments arranged by size:
3254 1572 756 575
ApaI G_GGCC'C
Cuts at: 2868 2868
Size: 6157
ApaLI G'TGCA_C
Cuts at: 4214 5370 4214
Size: 1156 5001
ApoI r'AATT_y
Cuts at: 1493 2485 2951 3466 3692 3844 5953 1493
Size: 992 466 515 226 152 2109 1697
Fragments arranged by size:
2109 1697 992 515 466 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4711 160
Size: 872 3679 1606
Fragments arranged by size:
3679 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6157
AvaI C'yCGr_G
Cuts at: 1870 1943 2128 2469 2537 3275 3482 1870
Size: 73 185 341 68 738 207 4545
Fragments arranged by size:
4545 738 341 207 185 73 68
BamHI G'GATC_C
Cuts at: 3487 3854 3487
Size: 367 5790
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2212 2435 2504 2532 2757
Size: 325 217 627 425 223 69 28 225
Cuts at: 2757 3020 3478 4933 618
Size: 263 458 1455 1842
Fragments arranged by size:
1842 1455 627 458 425 325 263 225
223 217 69 28
BanII G_rGCy'C
Cuts at: 1391 2135 2301 2652 2868 3276 3392 3476 1391
Size: 744 166 351 216 408 116 84 4072
Fragments arranged by size:
4072 744 408 351 216 166 116 84
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2548 928
Size: 971 649 4537
Fragments arranged by size:
4537 971 649
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4348 4382 1903
Size: 34 2411 34 3678
Fragments arranged by size:
3678 2411 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2021 4049 5259 2021
Size: 2028 1210 2919
Fragments arranged by size:
2919 2028 1210
BclI T'GATC_A
Cuts at: 1548 2778 1548
Size: 1230 4927
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4768 136
Size: 107 122 71 631 605 3096 1525
Fragments arranged by size:
3096 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2158 6152 2158
Size: 3994 2163
BlpI GC'TnA_GC
Cuts at: 3188 3574 3188
Size: 386 5771
Bme1580I G_kGCm'C
Cuts at: 1790 2049 2440 2754 2868 3025 4218 5374 1790
Size: 259 391 314 114 157 1193 1156 2573
Fragments arranged by size:
2573 1193 1156 391 314 259 157 114
BmgBI CAC'GTC
Cuts at: 1318 2193 1318
Size: 875 5282
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3888 4846 442
Size: 416 53 83 399 24 2471 958 1753
Fragments arranged by size:
2471 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6157
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 6157
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2368 2856 4817 1239
Size: 1129 488 1961 2579
Fragments arranged by size:
2579 1961 1129 488
Bpu10I CC'TnA_GC
Cuts at: 2208 2208
Size: 6157
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3308 4282 5147 5445 3308
Size: 974 865 298 4020
Fragments arranged by size:
4020 974 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4820 882
Size: 617 3321 2219
Fragments arranged by size:
3321 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3253 3948 493
Size: 711 2049 695 2702
Fragments arranged by size:
2702 2049 711 695
BsaBI GATnn'nnATC
Cuts at: 6151 6151
Size: 6157
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3964 4346 4981 275
Size: 53 83 186 936 2431 382 635 1451
Fragments arranged by size:
2431 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4590 4988 5262 5409 5820 4590
Size: 398 274 147 411 4927
Fragments arranged by size:
4927 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2147 1178
Size: 30 165 30 714 30 5188
Fragments arranged by size:
5188 714 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3143 3368 1186
Size: 1957 225 3975
Fragments arranged by size:
3975 1957 225
BseYI C'CCAG_C
Cuts at: 2110 2511 2620 2667 3423 5360 5856 2110
Size: 401 109 47 756 1937 496 2411
Fragments arranged by size:
2411 1937 756 496 401 109 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5584
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3607 4368 5396 1540
Size: 275 186 1606 761 1028 2301
Fragments arranged by size:
2301 1606 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4218 4303 5374 4218
Size: 85 1071 5001
Fragments arranged by size:
5001 1071 85
BsmI GAATG_Cn'
Cuts at: 3668 3761 3668
Size: 93 6064
BsmBI CGTCTCn'nnnn_
Cuts at: 2183 2183
Size: 6157
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2135 2301 2440 2652 2754 2868
Size: 399 259 86 166 139 212 102 114
Cuts at: 2868 3025 3276 3392 3476 4218 4303 5374 1391
Size: 157 251 116 84 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 259 251 212 166
157 139 116 114 102 86 85 84
BspEI T'CCGG_A
Cuts at: 5820 5820
Size: 6157
BspHI T'CATG_A
Cuts at: 4046 5776 4046
Size: 1730 4427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3512 844
Size: 2668 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 3195 4044 1394
Size: 429 188 1184 849 3507
Fragments arranged by size:
3507 1184 849 429 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 3081 4652 4826 65
Size: 1183 1833 1571 174 1396
Fragments arranged by size:
1833 1571 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3162 3339 4801 4988 1700
Size: 110 1352 177 1462 187 2869
Fragments arranged by size:
2869 1462 1352 187 177 110
BsrGI T'GTAC_A
Cuts at: 96 3255 96
Size: 3159 2998
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6157
BssSI C'ACGA_G
Cuts at: 4217 5229 4217
Size: 1012 5145
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6157
BstBI TT'CG_AA
Cuts at: 4961 4961
Size: 6157
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6157
BstXI CCAn_nnnn'nTGG
Cuts at: 3431 3431
Size: 6157
BstYI r'GATC_y
Cuts at: 1972 2158 2170 2495 3487 3854 4239 4256 5708
Size: 186 12 325 992 367 385 17 1452
Cuts at: 5708 5794 5806 6152 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 992 385 367 346 325 186
86 17 12 12
Bsu36I CC'TnA_GG
Cuts at: 3126 3126
Size: 6157
BtgI C'CryG_G
Cuts at: 513 1065 2004 2362 2527 2727 2886 513
Size: 552 939 358 165 200 159 3784
Fragments arranged by size:
3784 939 552 358 200 165 159
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5418
Fragments arranged by size:
5418 683 56
BtsI GCAGTG_nn'
Cuts at: 2378 3673 4467 4487 2378
Size: 1295 794 20 4048
Fragments arranged by size:
4048 1295 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6157
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6122
DraI TTT'AAA
Cuts at: 3461 3461
Size: 6157
DraIII CAC_nnn'GTG
Cuts at: 2826 2826
Size: 6157
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2816 3344 5164 809
Size: 507 1500 528 1820 1802
Fragments arranged by size:
1820 1802 1500 528 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2524 3164 3604
Size: 54 1425 50 275 18 694 640 440
Cuts at: 3604 4493 6122 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 694 640 440 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3604 1537
Size: 275 1792 4090
Fragments arranged by size:
4090 1792 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2168 3290 4087 1353
Size: 815 1122 797 3423
Fragments arranged by size:
3423 1122 815 797
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4744 5118 5264 387
Size: 1064 138 3155 374 146 1280
Fragments arranged by size:
3155 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2175 2223 2368 2856 4220 4817
Size: 393 409 134 48 145 488 1364 597
Cuts at: 4817 5604 5872 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1364 787 597 488 409 393 268
145 134 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2335 2335
Size: 6157
EcoO109I rG'GnC_Cy
Cuts at: 2865 3280 2865
Size: 415 5742
EcoRI G'AATT_C
Cuts at: 3466 3844 3466
Size: 378 5779
FspI TGC'GCA
Cuts at: 2830 4663 2830
Size: 1833 4324
FspAI rTGC'GCAy
Cuts at: 2830 2830
Size: 6157
HaeII r_GCGC'y
Cuts at: 1678 2099 3016 5304 1678
Size: 421 917 2288 2531
Fragments arranged by size:
2531 2288 917 421
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 4798 4830 4872
Size: 32 135 32 545 32 2649 32 42
Cuts at: 4872 4904 1373
Size: 32 2626
Fragments arranged by size:
2649 2626 545 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3501 3747 669
Size: 988 281 1563 246 3079
Fragments arranged by size:
3079 1563 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6157
HpaI GTT'AAC
Cuts at: 3747 3747
Size: 6157
KpnI G_GTAC'C
Cuts at: 2216 2536 2761 3482 2216
Size: 320 225 721 4891
Fragments arranged by size:
4891 721 320 225
MfeI C'AATT_G
Cuts at: 3756 3756
Size: 6157
MluI A'CGCG_T
Cuts at: 6033 6033
Size: 6157
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2625 5271 5455 1305
Size: 1320 2646 184 2007
Fragments arranged by size:
2646 2007 1320 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2526 10
Size: 54 1425 343 694 3641
Fragments arranged by size:
3641 1425 694 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3381 4115 4474 4633 518
Size: 648 2215 734 359 159 2042
Fragments arranged by size:
2215 2042 734 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2110 2641 3031 3573 4250 5398 5643 1606
Size: 504 531 390 542 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 542 531 504 390 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2362 2527 2886 513
Size: 552 939 358 165 359 3784
Fragments arranged by size:
3784 939 552 359 358 165
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6157
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6157
NotI GC'GGCC_GC
Cuts at: 3604 3604
Size: 6157
NspI r_CATG'y
Cuts at: 1132 1229 3515 5060 1132
Size: 97 2286 1545 2229
Fragments arranged by size:
2286 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2762 1348
Size: 1414 4743
PciI A'CATG_T
Cuts at: 5056 5056
Size: 6157
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3144 1108
Size: 2036 4121
PfoI T'CCnGG_A
Cuts at: 2166 2184 3300 2166
Size: 18 1116 5023
Fragments arranged by size:
5023 1116 18
PmeI GTTT'AAAC
Cuts at: 3461 3461
Size: 6157
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4226 4258 5739 5771 4226
Size: 32 1481 32 4612
Fragments arranged by size:
4612 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6157
PsiI TTA'TAA
Cuts at: 3727 3727
Size: 6157
PspOMI G'GGCC_C
Cuts at: 2864 2864
Size: 6157
PstI C_TGCA'G
Cuts at: 830 2336 3030 3509 830
Size: 1506 694 479 3478
Fragments arranged by size:
3478 1506 694 479
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6157
PvuII CAG'CTG
Cuts at: 2641 3031 2641
Size: 390 5767
SalI G'TCGA_C
Cuts at: 1936 3499 1936
Size: 1563 4594
SbfI CC_TGCA'GG
Cuts at: 3509 3509
Size: 6157
ScaI AGT'ACT
Cuts at: 4405 6117 4405
Size: 1712 4445
SfcI C'TryA_G
Cuts at: 826 1045 2332 2456 3026 3505 4640 5321 5512
Size: 219 1287 124 570 479 1135 681 191
Cuts at: 5512 826
Size: 1471
Fragments arranged by size:
1471 1287 1135 681 570 479 219 191
124
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6157
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6157
SmaI CCC'GGG
Cuts at: 1872 2130 2471 3484 1872
Size: 258 341 1013 4545
Fragments arranged by size:
4545 1013 341 258
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3323 4261 5162 5424 820
Size: 197 926 1380 938 901 262 1553
Fragments arranged by size:
1553 1380 938 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3948 493
Size: 3455 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6157
SphI G_CATG'C
Cuts at: 1132 3515 1132
Size: 2383 3774
SspI AAT'ATT
Cuts at: 5 52 4081 5
Size: 47 4029 2081
Fragments arranged by size:
4029 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6157
StyI C'CwwG_G
Cuts at: 513 1065 2004 2362 2440 2527 2886 3438 3596
Size: 552 939 358 78 87 359 552 158
Cuts at: 3596 513
Size: 3074
Fragments arranged by size:
3074 939 552 552 359 358 158 87
78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4184 4201 4354 4539 4984 325
Size: 1255 2604 17 153 185 445 1498
Fragments arranged by size:
2604 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3255 4403 6115
Size: 274 80 33 51 1443 1278 1148 1712
Cuts at: 6115 96
Size: 138
Fragments arranged by size:
1712 1443 1278 1148 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6117 6149 6117
Size: 32 6125
Tth111I GACn'n_nGTC
Cuts at: 1316 3362 1316
Size: 2046 4111
XbaI T'CTAG_A
Cuts at: 3493 3493
Size: 6157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2519 2525 2519
Size: 6 6151
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6157
XmaI C'CCGG_G
Cuts at: 1870 2128 2469 3482 1870
Size: 258 341 1013 4545
Fragments arranged by size:
4545 1013 341 258
XmnI GAAnn'nnTTC
Cuts at: 4286 4286
Size: 6157
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3965 276
Size: 53 83 186 936 2431 2468
Fragments arranged by size:
2468 2431 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BstZ17I EcoICRI EcoRV FalI FseI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI