Restriction map for
pFN21AB7738
(Linear) (Six-base) MAPSORT of: KIBB7738.seq Check: 9261 from: 1 to: 1452
KIBB7738.orf
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
Acc65I G'GTAC_C
Cuts at: 0 207 527 752 1452
Size: 207 320 225 700
Fragments arranged by size:
700 320 225 207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 170 218 1452
Size: 36 134 48 1234
Fragments arranged by size:
1234 134 48 36
AfeI AGC'GCT
Cuts at: 0 92 1452
Size: 92 1360
AflIII A'CryG_T
Cuts at: 0 1247 1452
Size: 1247 205
AlwNI CAG_nnn'CTG
Cuts at: 0 564 1139 1452
Size: 564 575 313
Fragments arranged by size:
575 564 313
ApaI G_GGCC'C
Cuts at: 0 863 1452
Size: 863 589
ApoI r'AATT_y
Cuts at: 0 480 946 1452
Size: 480 466 506
Fragments arranged by size:
506 480 466
AvaI C'yCGr_G
Cuts at: 0 123 464 532 1270 1452
Size: 123 341 68 738 182
Fragments arranged by size:
738 341 182 123 68
BanI G'GyrC_C
Cuts at: 0 207 430 499 527 752 1015 1452
Size: 207 223 69 28 225 263 437
Fragments arranged by size:
437 263 225 223 207 69 28
BanII G_rGCy'C
Cuts at: 0 130 296 647 863 1271 1387 1452
Size: 130 166 351 216 408 116 65
Fragments arranged by size:
408 351 216 166 130 116 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 543 1452
Size: 543 909
BciVI GTATCCnnnnn_n'
Cuts at: 0 16 1452
Size: 16 1436
BclI T'GATC_A
Cuts at: 0 773 1452
Size: 773 679
BglII A'GATC_T
Cuts at: 0 153 1452
Size: 153 1299
BlpI GC'TnA_GC
Cuts at: 0 1183 1452
Size: 1183 269
Bme1580I G_kGCm'C
Cuts at: 0 44 435 749 863 1020 1452
Size: 44 391 314 114 157 432
Fragments arranged by size:
432 391 314 157 114 44
BmgBI CAC'GTC
Cuts at: 0 188 1452
Size: 188 1264
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 870 1452
Size: 870 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 851 1452
Size: 363 488 601
Fragments arranged by size:
601 488 363
Bpu10I CC'TnA_GC
Cuts at: 0 203 1452
Size: 203 1249
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1303 1452
Size: 1303 149
BsaAI yAC'GTr
Cuts at: 0 1248 1452
Size: 1248 204
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 112 142 1452
Size: 112 30 1310
Fragments arranged by size:
1310 112 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1138 1363 1452
Size: 1138 225 89
Fragments arranged by size:
1138 225 89
BseYI C'CCAG_C
Cuts at: 0 105 506 615 662 1418 1452
Size: 105 401 109 47 756 34
Fragments arranged by size:
756 401 109 105 47 34
BsmBI CGTCTCn'nnnn_
Cuts at: 0 178 1452
Size: 178 1274
Bsp1286I G_dGCh'C
Cuts at: 0 44 130 296 435 647 749 863 1020
Size: 44 86 166 139 212 102 114 157
Cuts at: 1020 1271 1387 1452
Size: 251 116 65
Fragments arranged by size:
251 212 166 157 139 116 114 102
86 65 44
BsrBI CCG'CTC
Cuts at: 0 6 1190 1452
Size: 6 1184 262
Fragments arranged by size:
1184 262 6
BsrDI GCAATG_nn'
Cuts at: 0 1076 1452
Size: 1076 376
BsrFI r'CCGG_y
Cuts at: 0 1157 1334 1452
Size: 1157 177 118
Fragments arranged by size:
1157 177 118
BsrGI T'GTAC_A
Cuts at: 0 1250 1452
Size: 1250 202
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1426 1452
Size: 1426 26
BstYI r'GATC_y
Cuts at: 0 153 165 490 1452
Size: 153 12 325 962
Fragments arranged by size:
962 325 153 12
Bsu36I CC'TnA_GG
Cuts at: 0 1121 1452
Size: 1121 331
BtgI C'CryG_G
Cuts at: 0 357 522 722 881 1452
Size: 357 165 200 159 571
Fragments arranged by size:
571 357 200 165 159
BtsI GCAGTG_nn'
Cuts at: 0 373 1452
Size: 373 1079
DraIII CAC_nnn'GTG
Cuts at: 0 821 1452
Size: 821 631
DrdI GACnn_nn'nnGTC
Cuts at: 0 811 1339 1452
Size: 811 528 113
Fragments arranged by size:
811 528 113
EaeI y'GGCC_r
Cuts at: 0 519 1159 1452
Size: 519 640 293
Fragments arranged by size:
640 519 293
EarI CTCTTCn'nnn_
Cuts at: 0 163 1285 1452
Size: 163 1122 167
Fragments arranged by size:
1122 167 163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 170 218 363 851 1452
Size: 36 134 48 145 488 601
Fragments arranged by size:
601 488 145 134 48 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 330 1452
Size: 330 1122
EcoO109I rG'GnC_Cy
Cuts at: 0 860 1275 1452
Size: 860 415 177
Fragments arranged by size:
860 415 177
FspI TGC'GCA
Cuts at: 0 825 1452
Size: 825 627
FspAI rTGC'GCAy
Cuts at: 0 825 1452
Size: 825 627
HaeII r_GCGC'y
Cuts at: 0 94 1011 1452
Size: 94 917 441
Fragments arranged by size:
917 441 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 1452
Size: 112 32 1308
Fragments arranged by size:
1308 112 32
KpnI G_GTAC'C
Cuts at: 0 211 531 756 1452
Size: 211 320 225 696
Fragments arranged by size:
696 320 225 211
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 1452
Size: 620 832
MscI TGG'CCA
Cuts at: 0 521 1452
Size: 521 931
MslI CAynn'nnrTG
Cuts at: 0 1376 1452
Size: 1376 76
MspA1I CmG'CkG
Cuts at: 0 105 636 1026 1452
Size: 105 531 390 426
Fragments arranged by size:
531 426 390 105
NcoI C'CATG_G
Cuts at: 0 357 522 881 1452
Size: 357 165 359 571
Fragments arranged by size:
571 359 357 165
PasI CC'CwG_GG
Cuts at: 0 757 1452
Size: 757 695
PflMI CCAn_nnn'nTGG
Cuts at: 0 1139 1452
Size: 1139 313
PfoI T'CCnGG_A
Cuts at: 0 161 179 1295 1452
Size: 161 18 1116 157
Fragments arranged by size:
1116 161 157 18
PspOMI G'GGCC_C
Cuts at: 0 859 1452
Size: 859 593
PstI C_TGCA'G
Cuts at: 0 331 1025 1452
Size: 331 694 427
Fragments arranged by size:
694 427 331
PvuII CAG'CTG
Cuts at: 0 636 1026 1452
Size: 636 390 426
Fragments arranged by size:
636 426 390
SfcI C'TryA_G
Cuts at: 0 327 451 1021 1452
Size: 327 124 570 431
Fragments arranged by size:
570 431 327 124
SmaI CCC'GGG
Cuts at: 0 125 466 1452
Size: 125 341 986
Fragments arranged by size:
986 341 125
SmlI C'TyrA_G
Cuts at: 0 1318 1452
Size: 1318 134
StyI C'CwwG_G
Cuts at: 0 357 435 522 881 1433 1452
Size: 357 78 87 359 552 19
Fragments arranged by size:
552 359 357 87 78 19
TatI w'GTAC_w
Cuts at: 0 1250 1452
Size: 1250 202
Tth111I GACn'n_nGTC
Cuts at: 0 1357 1452
Size: 1357 95
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 514 520 1452
Size: 514 6 932
Fragments arranged by size:
932 514 6
XmaI C'CCGG_G
Cuts at: 0 123 464 1452
Size: 123 341 988
Fragments arranged by size:
988 341 123
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AlwNI ApaI ApoI AvaI
BanI BanII BbsI BciVI BclI BglII BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI
BseYI BsmBI Bsp1286I BsrBI BsrDI BsrFI BsrGI BstXI
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI
Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I KpnI
MmeI MscI MslI MspA1I NcoI PasI PflMI PfoI
PspOMI PstI PvuII SfcI SmaI SmlI StyI TatI
Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BfrBI BglI BmrI BmtI
BsaI BsaBI BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI
BsmI BspEI BspHI BspMI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI HincII HindIII HpaI
KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TstI XbaI XhoI XmnI
ZraI