(Circular) (Six-base) MAPSORT of: pFN21AB7713.seq Check: 6358 from: 1 to: 5974 pFN21AB7713 5974 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 4101 4474 1563 Size: 2538 373 3063 Fragments arranged by size: 3063 2538 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4037 5421 5689 1632 Size: 2405 1384 268 1917 Fragments arranged by size: 2405 1917 1384 268 AfeI AGC'GCT Cuts at: 2831 2831 Size: 5974 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 4873 5850 1203 Size: 3670 977 1327 Fragments arranged by size: 3670 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 2776 4703 2776 Size: 1927 4047 AleI CACnn'nnGTG Cuts at: 1166 2277 1166 Size: 1111 4863 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3164 1184 Size: 1980 3994 AlwNI CAG_nnn'CTG Cuts at: 2555 2657 2959 3020 3717 5289 2555 Size: 102 302 61 697 1572 3240 Fragments arranged by size: 3240 1572 697 302 102 61 ApaI G_GGCC'C Cuts at: 2528 2544 2528 Size: 16 5958 ApaLI G'TGCA_C Cuts at: 3023 4031 5187 3023 Size: 1008 1156 3810 Fragments arranged by size: 3810 1156 1008 ApoI r'AATT_y Cuts at: 1493 3283 3509 3661 5770 1493 Size: 1790 226 152 2109 1697 Fragments arranged by size: 2109 1790 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 2521 3257 3299 1870 Size: 73 578 736 42 4545 Fragments arranged by size: 4545 736 578 73 42 BamHI G'GATC_C Cuts at: 3304 3671 3304 Size: 367 5607 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2664 3226 3295 4750 618 Size: 325 217 627 877 562 69 1455 1842 Fragments arranged by size: 1842 1455 877 627 562 325 217 69 BanII G_rGCy'C Cuts at: 1391 2528 2544 3293 1391 Size: 1137 16 749 4072 Fragments arranged by size: 4072 1137 749 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2576 928 Size: 971 677 4326 Fragments arranged by size: 4326 971 677 BbvCI CC'TCA_GC Cuts at: 2865 2865 Size: 5974 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4165 4199 1903 Size: 34 2228 34 3678 Fragments arranged by size: 3678 2228 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3866 5076 3866 Size: 1210 4764 BclI T'GATC_A Cuts at: 1548 1548 Size: 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2148 2399 3101 Size: 107 122 71 631 605 476 251 702 Cuts at: 3101 3105 4585 136 Size: 4 1480 1525 Fragments arranged by size: 1525 1480 702 631 605 476 251 122 107 71 4 BglII A'GATC_T Cuts at: 5969 5969 Size: 5974 BlpI GC'TnA_GC Cuts at: 2216 3123 3391 2216 Size: 907 268 4799 Fragments arranged by size: 4799 907 268 Bme1580I G_kGCm'C Cuts at: 1790 2528 2544 2667 2896 3027 3231 4035 5191 Size: 738 16 123 229 131 204 804 1156 Cuts at: 5191 1790 Size: 2573 Fragments arranged by size: 2573 1156 804 738 229 204 131 123 16 BmgBI CAC'GTC Cuts at: 1318 2257 1318 Size: 939 5035 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2576 2927 3705 Size: 416 53 83 399 24 1159 351 778 Cuts at: 3705 4663 442 Size: 958 1753 Fragments arranged by size: 1753 1159 958 778 416 399 351 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2577 2987 3104 3236 4634 1239 Size: 1338 410 117 132 1398 2579 Fragments arranged by size: 2579 1398 1338 410 132 117 Bpu10I CC'TnA_GC Cuts at: 2865 2865 Size: 5974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2423 4099 4964 5262 2423 Size: 1676 865 298 3135 Fragments arranged by size: 3135 1676 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2087 4637 882 Size: 617 588 2550 2219 Fragments arranged by size: 2550 2219 617 588 BsaAI yAC'GTr Cuts at: 493 1204 3765 493 Size: 711 2561 2702 Fragments arranged by size: 2702 2561 711 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3781 4163 4798 275 Size: 53 83 186 936 2248 382 635 1451 Fragments arranged by size: 2248 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2086 2116 2246 2276 3150 Size: 30 165 30 683 30 130 30 874 Cuts at: 3150 3180 1178 Size: 30 3972 Fragments arranged by size: 3972 874 683 165 130 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2990 3155 1186 Size: 991 813 165 4005 Fragments arranged by size: 4005 991 813 165 BseYI C'CCAG_C Cuts at: 2180 2537 2671 2727 2815 2826 5177 5673 2180 Size: 357 134 56 88 11 2351 496 2481 Fragments arranged by size: 2481 2351 496 357 134 88 56 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3053 1163 Size: 573 1317 4084 Fragments arranged by size: 4084 1317 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2498 3100 3424 4185 5213 1540 Size: 275 186 497 602 324 761 1028 2301 Fragments arranged by size: 2301 1028 761 602 497 324 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 3027 4035 4120 5191 2217 Size: 810 1008 85 1071 3000 Fragments arranged by size: 3000 1071 1008 810 85 BsmI GAATG_Cn' Cuts at: 2593 3485 3578 2593 Size: 892 93 4989 Fragments arranged by size: 4989 892 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2377 2377 Size: 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2528 2544 2667 2896 3027 3231 Size: 399 427 311 16 123 229 131 204 Cuts at: 3231 3293 4035 4120 5191 1391 Size: 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 427 399 311 229 204 131 123 85 62 16 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3329 844 Size: 2485 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3127 3163 3861 1394 Size: 429 1304 36 698 3507 Fragments arranged by size: 3507 1304 698 429 36 BsrDI GCAATG_nn' Cuts at: 65 1248 4469 4643 65 Size: 1183 3221 174 1396 Fragments arranged by size: 3221 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2053 3095 4618 4805 1700 Size: 110 243 1042 1523 187 2869 Fragments arranged by size: 2869 1523 1042 243 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 3071 4034 5046 3071 Size: 963 1012 3999 Fragments arranged by size: 3999 1012 963 BstAPI GCAn_nnn'nTGC Cuts at: 794 2394 2941 2959 794 Size: 1600 547 18 3809 Fragments arranged by size: 3809 1600 547 18 BstBI TT'CG_AA Cuts at: 4778 4778 Size: 5974 BstEII G'GTnAC_C Cuts at: 1184 2716 1184 Size: 1532 4442 BstXI CCAn_nnnn'nTGG Cuts at: 2017 3203 2017 Size: 1186 4788 BstYI r'GATC_y Cuts at: 1972 3304 3671 4056 4073 5525 5611 5623 5969 Size: 1332 367 385 17 1452 86 12 346 Cuts at: 5969 1972 Size: 1977 Fragments arranged by size: 1977 1452 1332 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2676 513 Size: 552 1611 3811 Fragments arranged by size: 3811 1611 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2739 487 Size: 683 56 1513 3722 Fragments arranged by size: 3722 1513 683 56 BtsI GCAGTG_nn' Cuts at: 2510 2834 2840 3490 4284 4304 2510 Size: 324 6 650 794 20 4180 Fragments arranged by size: 4180 794 650 324 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DraIII CAC_nnn'GTG Cuts at: 2418 2418 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 2495 3097 Size: 54 1425 50 275 18 225 440 602 Cuts at: 3097 3421 4310 5939 8 Size: 324 889 1629 43 Fragments arranged by size: 1629 1425 889 602 440 324 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2495 3097 3421 1537 Size: 275 683 602 324 4090 Fragments arranged by size: 4090 683 602 324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 2880 3904 1353 Size: 801 726 1024 3423 Fragments arranged by size: 3423 1024 801 726 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3158 4561 4935 5081 387 Size: 1064 138 1569 1403 374 146 1280 Fragments arranged by size: 1569 1403 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2577 2987 3104 3236 4037 4634 5421 Size: 393 945 410 117 132 801 597 787 Cuts at: 5421 5689 1239 Size: 268 1524 Fragments arranged by size: 1524 945 801 787 597 410 393 268 132 117 EcoO109I rG'GnC_Cy Cuts at: 2525 2541 2561 2654 2956 3076 2525 Size: 16 20 93 302 120 5423 Fragments arranged by size: 5423 302 120 93 20 16 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3262 3262 Size: 5974 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 2236 2833 5121 1678 Size: 558 597 2288 2531 Fragments arranged by size: 2531 2288 597 558 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2086 2118 2246 2278 2762 Size: 32 135 32 514 32 128 32 484 Cuts at: 2762 2794 4615 4647 4689 4721 1373 Size: 32 1821 32 42 32 2626 Fragments arranged by size: 2626 1821 514 484 135 128 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3318 3564 669 Size: 988 281 1380 246 3079 Fragments arranged by size: 3079 1380 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5974 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2382 5088 5272 1305 Size: 1077 2706 184 2007 Fragments arranged by size: 2706 2007 1077 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4152 Fragments arranged by size: 4152 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2277 3201 3932 4291 4450 518 Size: 648 1111 924 731 359 159 2042 Fragments arranged by size: 2042 1111 924 731 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2346 2727 2903 3055 3179 3390 4067 5215 Size: 740 381 176 152 124 211 677 1148 Cuts at: 5215 5460 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 740 677 381 245 211 176 152 124 NaeI GCC'GGC Cuts at: 2055 3097 2055 Size: 1042 4932 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5422 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NgoMIV G'CCGG_C Cuts at: 2053 3095 2053 Size: 1042 4932 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 2437 3332 4877 1132 Size: 97 1208 895 1545 2229 Fragments arranged by size: 2229 1545 1208 895 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5974 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 2020 2727 1108 Size: 912 707 4355 Fragments arranged by size: 4355 912 707 PfoI T'CCnGG_A Cuts at: 2877 2877 Size: 5974 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4043 4075 5556 5588 4043 Size: 32 1481 32 4429 Fragments arranged by size: 4429 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 3544 3544 Size: 5974 PspOMI G'GGCC_C Cuts at: 2524 2540 2524 Size: 16 5958 PspXI vC'TCGA_Gb Cuts at: 3257 3257 Size: 5974 PstI C_TGCA'G Cuts at: 830 2790 2847 2955 3326 830 Size: 1960 57 108 371 3478 Fragments arranged by size: 3478 1960 371 108 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2346 2727 2903 3055 2346 Size: 381 176 152 5265 Fragments arranged by size: 5265 381 176 152 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 2784 4222 5934 2784 Size: 1438 1712 2824 Fragments arranged by size: 2824 1712 1438 SfcI C'TryA_G Cuts at: 826 1045 2786 2843 2951 3322 4457 5138 5329 Size: 219 1741 57 108 371 1135 681 191 Cuts at: 5329 826 Size: 1471 Fragments arranged by size: 1741 1471 1135 681 371 219 191 108 57 SfiI GGCCn_nnn'nGGCC Cuts at: 2399 3105 2399 Size: 706 5268 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 2523 3301 1872 Size: 651 778 4545 Fragments arranged by size: 4545 778 651 SmlI C'TyrA_G Cuts at: 820 1017 1943 2438 3257 4078 4979 5241 820 Size: 197 926 495 819 821 901 262 1553 Fragments arranged by size: 1553 926 901 821 819 495 262 197 SnaBI TAC'GTA Cuts at: 493 3765 493 Size: 3272 2702 SpeI A'CTAG_T Cuts at: 152 2938 152 Size: 2786 3188 SphI G_CATG'C Cuts at: 1132 2437 3332 1132 Size: 1305 895 3774 Fragments arranged by size: 3774 1305 895 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 2297 2393 2402 2996 1517 Size: 780 96 9 594 4495 Fragments arranged by size: 4495 780 594 96 9 StyI C'CwwG_G Cuts at: 513 1065 2997 3413 513 Size: 552 1932 416 3074 Fragments arranged by size: 3074 1932 552 416 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2111 2718 2807 4001 4018 4171 4356 Size: 1255 531 607 89 1194 17 153 185 Cuts at: 4356 4801 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1194 607 531 445 185 153 89 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2340 2484 2782 Size: 274 80 33 51 1443 363 144 298 Cuts at: 2782 4220 5932 96 Size: 1438 1712 138 Fragments arranged by size: 1712 1443 1438 363 298 274 144 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2502 2534 5934 5966 2502 Size: 32 3400 32 2510 Fragments arranged by size: 3400 2510 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2462 2873 1316 Size: 1146 411 4417 Fragments arranged by size: 4417 1146 411 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2017 2017 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 3257 1943 Size: 1314 4660 XmaI C'CCGG_G Cuts at: 1870 2521 3299 1870 Size: 651 778 4545 Fragments arranged by size: 4545 778 651 XmnI GAAnn'nnTTC Cuts at: 4103 4103 Size: 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI