Restriction map for
pFN21AE0357
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AspEI AspHI AspI AviII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HaeIII HapII
Hin1I HpaI HpaII KasI KspI
MamI MluI MluNI MroI MscI
MslI MspI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 275 GGTACC G^GTACC
AflIII 1278 ACGTGT A^C[GA][CT]GT
AlwNI 1583 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1668 CCTCAGG CC^T[ACGT]AGG
AseI 852 ATTAAT AT^TAAT
AsnI 852 ATTAAT AT^TAAT
Asp700I 1674 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 275 GGTACC G^GTACC
AvaI 575 CTCGGG C^[CT]CG[GA]G
AvrII 1555 CCTAGG C^CTAGG
BanI 275 GGTACC G^G[CT][GA]CC
BlnI 1555 CCTAGG C^CTAGG
BseRI 572 CTCCTC GAGGAG(10/8)
BsiYI 682 CCGTCACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 682 CCGTCACTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 739 GAGAC GTCTC(1/5)
BsoBI 575 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1221 TGTACA T^GTACA
BspHI 1055 TCATGA T^CATGA
BsrGI 1221 TGTACA T^GTACA
BstF5I 1299 GGATG GGATG(2/0)
Bsu36I 1668 CCTCAGG CC^T[ACGT]AGG
Cac8I 829 GCAAGC GC[ACGT]^[ACGT]GC
DraI 229 TTTAAA TTT^AAA
Eco81I 1668 CCTCAGG CC^T[ACGT]AGG
EcoRI 480 GAATTC G^AATTC
FokI 1299 GGATG GGATG(9/13)
HincII 1048 GTCAAC GT[CT]^[GA]AC
HindII 1048 GTCAAC GT[CT]^[GA]AC
HindIII 1335 AAGCTT A^AGCTT
KpnI 275 GGTACC GGTAC^C
MaeII 1278 ACGT A^CGT
MspA1I 551 CAGCTG C[AC]G^C[GT]G
MwoI 1218 GCATGTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 275 GGTACC GG[ACGT]^[ACGT]CC
NspI 1218 GCATGT [GA]CATG^[CT]
PshAI 1132 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 852 ATTAAT AT^TAAT
PstI 1589 CTGCAG CTGCA^G
PvuII 551 CAGCTG CAG^CTG
RcaI 1055 TCATGA T^CATGA
ScaI 1760 AGTACT AGT^ACT
SfcI 1589 CTGCAG C^T[GA][CT]AG
SspBI 1221 TGTACA T^GTACA
SwaI 228 ATTTAAAT ATTT^AAAT
XbaI 484 TCTAGA T^CTAGA
XmnI 1674 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcsI 480 GAATTC [GA]^AATT[CT]
489 AAATTC
AlwI 1330 GATCC GGATC(4/5)
1042 GGATC
ApoI 480 GAATTC [GA]^AATT[CT]
489 AAATTC
AvaII 792 GGTCC G^G[AT]CC
1525 GGTCC
BglII 316 AGATCT A^GATCT
1012 AGATCT
BpmI 613 CTCCAG CTGGAG(16/14)
289 CTGGAG
BsmFI 690 GGGAC GGGAC(10/14)
666 GTCCC
BspMI 137 ACCTGC ACCTGC(4/8)
1587 ACCTGC
BssKI 615 CCAGG ^CC[ACGT]GG
669 CCTGG
BstNI 615 CCAGG CC^[AT]GG
669 CCTGG
Cfr13I 792 GGTCC G^G[ACGT]CC
1525 GGTCC
EcoRII 615 CCAGG ^CC[AT]GG
669 CCTGG
EcoT14I 16 CCAAGG C^C[AT][AT]GG
1555 CCTAGG
HgaI 692 GACGC GACGC(5/10)
1132 GACGC
MfeI 521 CAATTG C^AATTG
1520 CAATTG
MunI 521 CAATTG C^AATTG
1520 CAATTG
MvaI 615 CCAGG CC^[AT]GG
669 CCTGG
Sau96I 792 GGTCC G^G[ACGT]CC
1525 GGTCC
ScrFI 615 CCAGG CC^[ACGT]GG
669 CCTGG
SfaNI 991 GCATC GCATC(5/9)
1227 GCATC
StyI 16 CCAAGG C^C[AT][AT]GG
1555 CCTAGG
TaqI 504 TCGA T^CGA
1130 TCGA
TthHB8I 504 TCGA T^CGA
1130 TCGA
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
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AfaI AluI BbvI BfaI BsaJI
BsrDI BsrI BstYI CfoI DdeI
DpnII EarI Fnu4HI HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI NdeII NlaIII RsaI Sau3AI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII