(Circular) (Six-base) MAPSORT of: pFN21AE0357.seq Check: 3998 from: 1 to: 6508 pFN21AE0357 6508 bp With 182 enzymes: SgfI * April 15, 2010 09:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4318 278 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3851 1937 Size: 1914 4594 Acc65I G'GTAC_C Cuts at: 2280 3829 2280 Size: 1549 4959 AclI AA'CG_TT Cuts at: 1563 4635 5008 1563 Size: 3072 373 3063 Fragments arranged by size: 3072 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2913 2974 2985 3228 3653 4571 5955 6223 Size: 1281 61 11 243 425 918 1384 268 Cuts at: 6223 1632 Size: 1917 Fragments arranged by size: 1917 1384 1281 918 425 268 243 61 11 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6311 AflIII A'CryG_T Cuts at: 1203 3283 5407 6384 1203 Size: 2080 2124 977 1327 Fragments arranged by size: 2124 2080 1327 977 AgeI A'CCGG_T Cuts at: 5339 5339 Size: 6508 AhdI GACnn_n'nnGTC Cuts at: 5237 5237 Size: 6508 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6508 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6508 AlwNI CAG_nnn'CTG Cuts at: 3593 4251 5823 3593 Size: 658 1572 4278 Fragments arranged by size: 4278 1572 658 ApaLI G'TGCA_C Cuts at: 4565 5721 4565 Size: 1156 5352 ApoI r'AATT_y Cuts at: 1493 2485 2494 3817 4043 4195 6304 1493 Size: 992 9 1323 226 152 2109 1697 Fragments arranged by size: 2109 1697 1323 992 226 152 9 AseI AT'TA_AT Cuts at: 160 1032 2858 5062 160 Size: 872 1826 2204 1606 Fragments arranged by size: 2204 1826 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 AvaI C'yCGr_G Cuts at: 1870 1943 2580 3833 1870 Size: 73 637 1253 4545 Fragments arranged by size: 4545 1253 637 73 AvrII C'CTAG_G Cuts at: 3560 3560 Size: 6508 BamHI G'GATC_C Cuts at: 3838 4205 3838 Size: 367 6141 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2280 3829 5284 618 Size: 325 217 627 493 1549 1455 1842 Fragments arranged by size: 1842 1549 1455 627 493 325 217 BanII G_rGCy'C Cuts at: 1391 3827 1391 Size: 2436 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5537 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4699 4733 1903 Size: 34 2762 34 3678 Fragments arranged by size: 3678 2762 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4400 5610 4400 Size: 1210 5298 BclI T'GATC_A Cuts at: 1548 1548 Size: 6508 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5119 136 Size: 107 122 71 631 605 3447 1525 Fragments arranged by size: 3447 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2321 3017 6503 2321 Size: 696 3486 2326 Fragments arranged by size: 3486 2326 696 BlpI GC'TnA_GC Cuts at: 3925 3925 Size: 6508 Bme1580I G_kGCm'C Cuts at: 1790 4569 5725 1790 Size: 2779 1156 2573 Fragments arranged by size: 2779 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6508 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2702 4239 5197 Size: 416 53 83 399 24 1285 1537 958 Cuts at: 5197 442 Size: 1753 Fragments arranged by size: 1753 1537 1285 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6508 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 2603 5168 1239 Size: 1076 288 2565 2579 Fragments arranged by size: 2579 2565 1076 288 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4633 5498 5796 4633 Size: 865 298 5345 Fragments arranged by size: 5345 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5171 882 Size: 617 3672 2219 Fragments arranged by size: 3672 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4299 493 Size: 711 3095 2702 Fragments arranged by size: 3095 2702 711 BsaBI GATnn'nnATC Cuts at: 6502 6502 Size: 6508 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4315 4697 5332 275 Size: 53 83 186 936 2782 382 635 1451 Fragments arranged by size: 2782 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4941 5339 5613 5760 6171 4941 Size: 398 274 147 411 5278 Fragments arranged by size: 5278 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2947 2977 1178 Size: 30 165 30 1544 30 4709 Fragments arranged by size: 4709 1544 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2568 1186 Size: 1382 5126 BseYI C'CCAG_C Cuts at: 5711 6207 5711 Size: 496 6012 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5935 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3958 4719 5747 1540 Size: 275 186 1957 761 1028 2301 Fragments arranged by size: 2301 1957 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4569 4654 5725 4569 Size: 85 1071 5352 Fragments arranged by size: 5352 1071 85 BsmI GAATG_Cn' Cuts at: 4019 4112 4019 Size: 93 6415 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3827 4569 4654 5725 1391 Size: 399 2037 742 85 1071 2174 Fragments arranged by size: 2174 2037 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6171 6171 Size: 6508 BspHI T'CATG_A Cuts at: 3060 4397 6127 3060 Size: 1337 1730 3441 Fragments arranged by size: 3441 1730 1337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2151 3601 3863 844 Size: 1307 1450 262 3489 Fragments arranged by size: 3489 1450 1307 262 BsrBI CCG'CTC Cuts at: 1394 1823 4395 1394 Size: 429 2572 3507 Fragments arranged by size: 3507 2572 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2146 3578 3648 5003 5177 65 Size: 1183 898 1432 70 1355 174 1396 Fragments arranged by size: 1432 1396 1355 1183 898 174 70 BsrFI r'CCGG_y Cuts at: 1700 1810 5152 5339 1700 Size: 110 3342 187 2869 Fragments arranged by size: 3342 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3226 96 Size: 3130 3378 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6508 BssSI C'ACGA_G Cuts at: 4568 5580 4568 Size: 1012 5496 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6508 BstBI TT'CG_AA Cuts at: 5312 5312 Size: 6508 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6508 BstYI r'GATC_y Cuts at: 1972 2321 3017 3047 3838 4205 4590 4607 6059 Size: 349 696 30 791 367 385 17 1452 Cuts at: 6059 6145 6157 6503 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 791 696 385 367 349 346 86 30 17 12 Bsu36I CC'TnA_GG Cuts at: 3674 3674 Size: 6508 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5017 Fragments arranged by size: 5017 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5769 Fragments arranged by size: 5769 683 56 BtsI GCAGTG_nn' Cuts at: 4024 4818 4838 4024 Size: 794 20 5694 Fragments arranged by size: 5694 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6508 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2258 2293 3160 3195 529 Size: 35 1694 35 867 35 3842 Fragments arranged by size: 3842 1694 867 35 35 35 DraI TTT'AAA Cuts at: 2236 3812 2236 Size: 1576 4932 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5515 809 Size: 507 4199 1802 Fragments arranged by size: 4199 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3955 4844 6473 Size: 54 1425 50 275 18 2125 889 1629 Cuts at: 6473 8 Size: 43 Fragments arranged by size: 2125 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3955 1537 Size: 275 2143 4090 Fragments arranged by size: 4090 2143 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 2423 2799 3629 4438 1353 Size: 668 402 376 830 809 3423 Fragments arranged by size: 3423 830 809 668 402 376 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5095 5469 5615 387 Size: 1064 138 3506 374 146 1280 Fragments arranged by size: 3506 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2315 2603 2913 2974 2985 3228 3653 Size: 393 683 288 310 61 11 243 425 Cuts at: 3653 4571 5168 5955 6223 1239 Size: 918 597 787 268 1524 Fragments arranged by size: 1524 918 787 683 597 425 393 310 288 268 243 61 11 EcoRI G'AATT_C Cuts at: 2485 3817 4195 2485 Size: 1332 378 4798 Fragments arranged by size: 4798 1332 378 FspI TGC'GCA Cuts at: 5014 5014 Size: 6508 HaeII r_GCGC'y Cuts at: 1678 5655 1678 Size: 3977 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2306 2338 2947 2979 5149 Size: 32 135 32 734 32 609 32 2170 Cuts at: 5149 5181 5223 5255 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2170 734 609 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3055 3852 4098 669 Size: 988 281 1117 797 246 3079 Fragments arranged by size: 3079 1117 988 797 281 246 HindIII A'AGCT_T Cuts at: 748 3340 748 Size: 2592 3916 HpaI GTT'AAC Cuts at: 4098 4098 Size: 6508 KpnI G_GTAC'C Cuts at: 2284 3833 2284 Size: 1549 4959 MfeI C'AATT_G Cuts at: 2526 3525 4107 2526 Size: 999 582 4927 Fragments arranged by size: 4927 999 582 MluI A'CGCG_T Cuts at: 6384 6384 Size: 6508 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3225 3251 5622 5806 1305 Size: 1920 26 2371 184 2007 Fragments arranged by size: 2371 2007 1920 184 26 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4686 Fragments arranged by size: 4686 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4466 4825 4984 518 Size: 648 3300 359 159 2042 Fragments arranged by size: 3300 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2558 3924 4601 5749 5994 1606 Size: 952 1366 677 1148 245 2120 Fragments arranged by size: 2120 1366 1148 952 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5017 Fragments arranged by size: 5017 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6508 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6508 NotI GC'GGCC_GC Cuts at: 3955 3955 Size: 6508 NspI r_CATG'y Cuts at: 1132 1229 3227 3866 5411 1132 Size: 97 1998 639 1545 2229 Fragments arranged by size: 2229 1998 1545 639 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6508 PciI A'CATG_T Cuts at: 5407 5407 Size: 6508 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6508 PmeI GTTT'AAAC Cuts at: 3812 3812 Size: 6508 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4577 4609 6090 6122 4577 Size: 32 1481 32 4963 Fragments arranged by size: 4963 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3141 1613 Size: 1528 4980 PsiI TTA'TAA Cuts at: 4078 4078 Size: 6508 PstI C_TGCA'G Cuts at: 830 3598 3860 830 Size: 2768 262 3478 Fragments arranged by size: 3478 2768 262 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6508 PvuII CAG'CTG Cuts at: 2558 2558 Size: 6508 SalI G'TCGA_C Cuts at: 1936 3850 1936 Size: 1914 4594 SbfI CC_TGCA'GG Cuts at: 3860 3860 Size: 6508 ScaI AGT'ACT Cuts at: 3767 4756 6468 3767 Size: 989 1712 3807 Fragments arranged by size: 3807 1712 989 SfcI C'TryA_G Cuts at: 826 1045 3594 3856 4991 5672 5863 826 Size: 219 2549 262 1135 681 191 1471 Fragments arranged by size: 2549 1471 1135 681 262 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6508 SmaI CCC'GGG Cuts at: 1872 3835 1872 Size: 1963 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4612 5513 5775 820 Size: 197 926 2669 901 262 1553 Fragments arranged by size: 2669 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4299 493 Size: 3806 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6508 SphI G_CATG'C Cuts at: 1132 3866 1132 Size: 2734 3774 SspI AAT'ATT Cuts at: 5 52 4432 5 Size: 47 4380 2081 Fragments arranged by size: 4380 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6508 StyI C'CwwG_G Cuts at: 513 1065 2004 2021 3560 3947 513 Size: 552 939 17 1539 387 3074 Fragments arranged by size: 3074 1539 939 552 387 17 SwaI ATTT'AAAT Cuts at: 2236 2236 Size: 6508 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2717 3462 4535 4552 4705 4890 5335 Size: 1255 1137 745 1073 17 153 185 445 Cuts at: 5335 325 Size: 1498 Fragments arranged by size: 1498 1255 1137 1073 745 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3226 3765 4754 Size: 274 80 33 51 1443 1249 539 989 Cuts at: 4754 6466 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1249 989 539 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2965 2997 6468 6500 2965 Size: 32 3471 32 2973 Fragments arranged by size: 3471 2973 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6508 XbaI T'CTAG_A Cuts at: 2489 3844 2489 Size: 1355 5153 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6508 XmaI C'CCGG_G Cuts at: 1870 3833 1870 Size: 1963 4545 XmnI GAAnn'nnTTC Cuts at: 3683 4637 3683 Size: 954 5554 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4316 276 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI