Restriction map for
pFN21AE0357
(Linear) (Six-base) MAPSORT of: KIEE0357.seq Check: 3640 from: 1 to: 1803
KIEE0357.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
Acc65I G'GTAC_C
Cuts at: 0 275 1803
Size: 275 1528
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 969 980 1223 1648 1803
Size: 908 61 11 243 425 155
Fragments arranged by size:
908 425 243 155 61 11
AflIII A'CryG_T
Cuts at: 0 1278 1803
Size: 1278 525
AlwNI CAG_nnn'CTG
Cuts at: 0 1588 1803
Size: 1588 215
ApoI r'AATT_y
Cuts at: 0 480 489 1803
Size: 480 9 1314
Fragments arranged by size:
1314 480 9
AseI AT'TA_AT
Cuts at: 0 853 1803
Size: 853 950
AvaI C'yCGr_G
Cuts at: 0 575 1803
Size: 575 1228
AvrII C'CTAG_G
Cuts at: 0 1555 1803
Size: 1555 248
BanI G'GyrC_C
Cuts at: 0 275 1803
Size: 275 1528
BglII A'GATC_T
Cuts at: 0 316 1012 1803
Size: 316 696 791
Fragments arranged by size:
791 696 316
BmrI ACTGGGnnnn_n'
Cuts at: 0 697 1803
Size: 697 1106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 598 1803
Size: 310 288 1205
Fragments arranged by size:
1205 310 288
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 942 972 1803
Size: 942 30 831
Fragments arranged by size:
942 831 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 563 1803
Size: 563 1240
BspHI T'CATG_A
Cuts at: 0 1055 1803
Size: 1055 748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 146 1596 1803
Size: 146 1450 207
Fragments arranged by size:
1450 207 146
BsrDI GCAATG_nn'
Cuts at: 0 141 1573 1643 1803
Size: 141 1432 70 160
Fragments arranged by size:
1432 160 141 70
BsrGI T'GTAC_A
Cuts at: 0 1221 1803
Size: 1221 582
BstYI r'GATC_y
Cuts at: 0 316 1012 1042 1803
Size: 316 696 30 761
Fragments arranged by size:
761 696 316 30
Bsu36I CC'TnA_GG
Cuts at: 0 1669 1803
Size: 1669 134
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 253 288 1155 1190 1803
Size: 253 35 867 35 613
Fragments arranged by size:
867 613 253 35 35
DraI TTT'AAA
Cuts at: 0 231 1803
Size: 231 1572
EarI CTCTTCn'nnn_
Cuts at: 0 16 418 794 1624 1803
Size: 16 402 376 830 179
Fragments arranged by size:
830 402 376 179 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 598 908 969 980 1223 1648 1803
Size: 310 288 310 61 11 243 425 155
Fragments arranged by size:
425 310 310 288 243 155 61 11
EcoRI G'AATT_C
Cuts at: 0 480 1803
Size: 480 1323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 301 333 942 974 1803
Size: 301 32 609 32 829
Fragments arranged by size:
829 609 301 32 32
HincII GTy'rAC
Cuts at: 0 1050 1803
Size: 1050 753
HindIII A'AGCT_T
Cuts at: 0 1335 1803
Size: 1335 468
KpnI G_GTAC'C
Cuts at: 0 279 1803
Size: 279 1524
MfeI C'AATT_G
Cuts at: 0 521 1520 1803
Size: 521 999 283
Fragments arranged by size:
999 521 283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1220 1246 1803
Size: 1220 26 557
Fragments arranged by size:
1220 557 26
MspA1I CmG'CkG
Cuts at: 0 553 1803
Size: 553 1250
NspI r_CATG'y
Cuts at: 0 1222 1803
Size: 1222 581
PshAI GACnn'nnGTC
Cuts at: 0 1136 1803
Size: 1136 667
PstI C_TGCA'G
Cuts at: 0 1593 1803
Size: 1593 210
PvuII CAG'CTG
Cuts at: 0 553 1803
Size: 553 1250
ScaI AGT'ACT
Cuts at: 0 1762 1803
Size: 1762 41
SfcI C'TryA_G
Cuts at: 0 1589 1803
Size: 1589 214
StyI C'CwwG_G
Cuts at: 0 16 1555 1803
Size: 16 1539 248
Fragments arranged by size:
1539 248 16
SwaI ATTT'AAAT
Cuts at: 0 231 1803
Size: 231 1572
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 712 1457 1803
Size: 712 745 346
Fragments arranged by size:
745 712 346
TatI w'GTAC_w
Cuts at: 0 1221 1760 1803
Size: 1221 539 43
Fragments arranged by size:
1221 539 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 960 992 1803
Size: 960 32 811
Fragments arranged by size:
960 811 32
XbaI T'CTAG_A
Cuts at: 0 484 1803
Size: 484 1319
XmnI GAAnn'nnTTC
Cuts at: 0 1678 1803
Size: 1678 125
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApoI AseI AvaI AvrII
BanI BglII BmrI BpmI BsaXI BseRI BspHI BspMI
BsrDI BsrGI BstYI Bsu36I CspCI DraI EarI Eco57MI
EcoRI Hin4I HincII HindIII KpnI MfeI MmeI MspA1I
NspI PshAI PstI PvuII ScaI SfcI StyI SwaI
TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI BaeI
BamHI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII HpaI KasI MluI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI Tth111I XcmI XhoI XmaI ZraI