(Circular) (Six-base) MAPSORT of: pFN21AB8348.seq Check: 7061 from: 1 to: 6142 pFN21AB8348 6142 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2469 3952 278 Size: 53 83 186 936 933 1483 2468 Fragments arranged by size: 2468 1483 936 933 186 83 53 AccI GT'mk_AC Cuts at: 1937 3485 1937 Size: 1548 4594 Acc65I G'GTAC_C Cuts at: 3463 3463 Size: 6142 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2601 2691 2739 2874 4205 5589 5857 1632 Size: 969 90 48 135 1331 1384 268 1917 Fragments arranged by size: 1917 1384 1331 969 268 135 90 48 AfeI AGC'GCT Cuts at: 3158 3158 Size: 6142 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 2152 5041 6018 1203 Size: 949 2889 977 1327 Fragments arranged by size: 2889 1327 977 949 AgeI A'CCGG_T Cuts at: 2786 4973 2786 Size: 2187 3955 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6142 AlwNI CAG_nnn'CTG Cuts at: 2431 3885 5457 2431 Size: 1454 1572 3116 Fragments arranged by size: 3116 1572 1454 ApaLI G'TGCA_C Cuts at: 4199 5355 4199 Size: 1156 4986 ApoI r'AATT_y Cuts at: 1493 3364 3451 3677 3829 5938 1493 Size: 1871 87 226 152 2109 1697 Fragments arranged by size: 2109 1871 1697 226 152 87 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 3467 1870 Size: 73 1524 4545 Fragments arranged by size: 4545 1524 73 BamHI G'GATC_C Cuts at: 3031 3472 3839 3031 Size: 441 367 5334 Fragments arranged by size: 5334 441 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3005 3298 3463 4918 618 Size: 325 217 627 1218 293 165 1455 1842 Fragments arranged by size: 1842 1455 1218 627 325 293 217 165 BanII G_rGCy'C Cuts at: 1391 2150 2766 2964 3263 3461 1391 Size: 759 616 198 299 198 4072 Fragments arranged by size: 4072 759 616 299 198 198 BbeI G_GCGC'C Cuts at: 3302 3302 Size: 6142 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2862 928 Size: 971 963 4208 Fragments arranged by size: 4208 971 963 BbvCI CC'TCA_GC Cuts at: 2160 2160 Size: 6142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2362 2396 4333 4367 1903 Size: 34 425 34 1937 34 3678 Fragments arranged by size: 3678 1937 425 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 1548 Size: 6142 BfrBI ATG'CAT Cuts at: 2662 2662 Size: 6142 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4753 136 Size: 107 122 71 631 605 3081 1525 Fragments arranged by size: 3081 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6137 6137 Size: 6142 BlpI GC'TnA_GC Cuts at: 3098 3559 3098 Size: 461 5681 Bme1580I G_kGCm'C Cuts at: 1790 2847 3434 4203 5359 1790 Size: 1057 587 769 1156 2573 Fragments arranged by size: 2573 1156 1057 769 587 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6142 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2803 3384 3873 Size: 416 53 83 399 24 1386 581 489 Cuts at: 3873 4831 442 Size: 958 1753 Fragments arranged by size: 1753 1386 958 581 489 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2101 2453 2968 2978 3185 3294 4802 1239 Size: 862 352 515 10 207 109 1508 2579 Fragments arranged by size: 2579 1508 862 515 352 207 109 10 Bpu10I CC'TnA_GC Cuts at: 2160 2160 Size: 6142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2221 4267 5132 5430 2221 Size: 2046 865 298 2933 Fragments arranged by size: 2933 2046 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4805 882 Size: 617 3306 2219 Fragments arranged by size: 3306 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3933 493 Size: 711 2729 2702 Fragments arranged by size: 2729 2702 711 BsaBI GATnn'nnATC Cuts at: 2880 6136 2880 Size: 3256 2886 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2466 3299 3949 4331 Size: 53 83 186 936 933 833 650 382 Cuts at: 4331 4966 275 Size: 635 1451 Fragments arranged by size: 1451 936 933 833 650 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2786 4575 4973 5247 5394 5805 2786 Size: 1789 398 274 147 411 3123 Fragments arranged by size: 3123 1789 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2951 2981 3245 3275 1178 Size: 30 165 30 1548 30 264 30 4045 Fragments arranged by size: 4045 1548 264 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2104 2345 2589 1186 Size: 918 241 244 4739 Fragments arranged by size: 4739 918 244 241 BseYI C'CCAG_C Cuts at: 2185 2546 3054 5345 5841 2185 Size: 361 508 2291 496 2486 Fragments arranged by size: 2486 2291 508 496 361 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2703 1163 Size: 573 967 4602 Fragments arranged by size: 4602 967 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3592 4353 5381 1540 Size: 275 186 1591 761 1028 2301 Fragments arranged by size: 2301 1591 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2964 4203 4288 5359 2964 Size: 1239 85 1071 3747 Fragments arranged by size: 3747 1239 1071 85 BsmI GAATG_Cn' Cuts at: 2261 3653 3746 2261 Size: 1392 93 4657 Fragments arranged by size: 4657 1392 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2150 2766 2847 2964 3263 3434 3461 Size: 399 360 616 81 117 299 171 27 Cuts at: 3461 4203 4288 5359 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 616 399 360 299 171 117 85 81 27 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 3135 4031 5761 3135 Size: 896 1730 3516 Fragments arranged by size: 3516 1730 896 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3497 844 Size: 2653 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3064 4029 1394 Size: 429 1241 965 3507 Fragments arranged by size: 3507 1241 965 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4637 4811 65 Size: 1183 3389 174 1396 Fragments arranged by size: 3389 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2786 4786 4973 1700 Size: 110 976 2000 187 2869 Fragments arranged by size: 2869 2000 976 187 110 BsrGI T'GTAC_A Cuts at: 96 3228 96 Size: 3132 3010 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6142 BstBI TT'CG_AA Cuts at: 4946 4946 Size: 6142 BstEII G'GTnAC_C Cuts at: 1184 2194 1184 Size: 1010 5132 BstXI CCAn_nnnn'nTGG Cuts at: 2206 2231 2206 Size: 25 6117 BstYI r'GATC_y Cuts at: 1972 2392 2875 2934 3031 3472 3839 4224 4241 Size: 420 483 59 97 441 367 385 17 Cuts at: 4241 5693 5779 5791 6137 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 483 441 420 385 367 346 97 86 59 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2651 3148 3181 513 Size: 552 939 647 497 33 3474 Fragments arranged by size: 3474 939 647 552 497 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5403 Fragments arranged by size: 5403 683 56 BtsI GCAGTG_nn' Cuts at: 2266 2432 2932 3658 4452 4472 2266 Size: 166 500 726 794 20 3936 Fragments arranged by size: 3936 794 726 500 166 20 ClaI AT'CG_AT Cuts at: 1554 2493 1554 Size: 939 5203 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 3446 3446 Size: 6142 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5149 809 Size: 507 3833 1802 Fragments arranged by size: 3833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2265 2794 Size: 54 1425 50 275 18 177 258 529 Cuts at: 2794 2929 3589 4478 6107 8 Size: 135 660 889 1629 43 Fragments arranged by size: 1629 1425 889 660 529 275 258 177 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 1537 Size: 275 1777 4090 Fragments arranged by size: 4090 1777 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2607 3062 4072 1353 Size: 1254 455 1010 3423 Fragments arranged by size: 3423 1254 1010 455 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4729 5103 5249 387 Size: 1064 138 3140 374 146 1280 Fragments arranged by size: 3140 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2962 2962 Size: 6142 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2101 2453 2601 2691 2739 2874 2968 Size: 393 469 352 148 90 48 135 94 Cuts at: 2968 2978 3185 3294 4205 4802 5589 5857 1239 Size: 10 207 109 911 597 787 268 1524 Fragments arranged by size: 1524 911 787 597 469 393 352 268 207 148 135 109 94 90 48 10 EcoO109I rG'GnC_Cy Cuts at: 3196 3196 Size: 6142 EcoRI G'AATT_C Cuts at: 3364 3451 3829 3364 Size: 87 378 5677 Fragments arranged by size: 5677 378 87 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2044 2545 3193 3307 2044 Size: 501 648 114 4879 Fragments arranged by size: 4879 648 501 114 FspI TGC'GCA Cuts at: 4648 4648 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 3055 3160 3302 5289 1678 Size: 1377 105 142 1987 2531 Fragments arranged by size: 2531 1987 1377 142 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2068 2159 2191 2951 Size: 32 135 32 464 32 91 32 760 Cuts at: 2951 2983 3016 3048 4783 4815 4857 4889 1373 Size: 32 33 32 1735 32 42 32 2626 Fragments arranged by size: 2626 1735 760 464 135 91 42 33 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2470 3486 3732 669 Size: 988 281 532 1016 246 3079 Fragments arranged by size: 3079 1016 988 532 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6142 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KasI G'GCGC_C Cuts at: 3298 3298 Size: 6142 KpnI G_GTAC'C Cuts at: 3467 3467 Size: 6142 MfeI C'AATT_G Cuts at: 3741 3741 Size: 6142 MluI A'CGCG_T Cuts at: 2152 6018 2152 Size: 3866 2276 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2949 3003 5256 5440 1305 Size: 1644 54 2253 184 2007 Fragments arranged by size: 2253 2007 1644 184 54 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2267 2931 10 Size: 54 1425 343 177 258 664 3221 Fragments arranged by size: 3221 1425 664 343 258 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 4100 4459 4618 518 Size: 648 1038 1896 359 159 2042 Fragments arranged by size: 2042 1896 1038 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2988 3558 4235 5383 5628 1606 Size: 1382 570 677 1148 245 2120 Fragments arranged by size: 2120 1382 1148 677 570 245 NarI GG'CG_CC Cuts at: 3299 3299 Size: 6142 NcoI C'CATG_G Cuts at: 513 1065 2004 3148 513 Size: 552 939 1144 3507 Fragments arranged by size: 3507 1144 939 552 NdeI CA'TA_TG Cuts at: 387 2758 3414 387 Size: 2371 656 3115 Fragments arranged by size: 3115 2371 656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6142 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NsiI A_TGCA'T Cuts at: 2664 2664 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 2344 2666 3500 5045 1132 Size: 97 1115 322 834 1545 2229 Fragments arranged by size: 2229 1545 1115 834 322 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6142 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 2927 1108 Size: 1819 4323 PfoI T'CCnGG_A Cuts at: 2955 2955 Size: 6142 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2862 3143 1613 Size: 1249 281 4612 Fragments arranged by size: 4612 1249 281 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PstI C_TGCA'G Cuts at: 830 3494 830 Size: 2664 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 PvuII CAG'CTG Cuts at: 2988 2988 Size: 6142 SacI G_AGCT'C Cuts at: 2964 2964 Size: 6142 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SapI GCTCTTCn'nnn_ Cuts at: 3062 3062 Size: 6142 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 4390 6102 4390 Size: 1712 4430 SfcI C'TryA_G Cuts at: 826 1045 3490 4625 5306 5497 826 Size: 219 2445 1135 681 191 1471 Fragments arranged by size: 2445 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3300 3300 Size: 6142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 3469 1872 Size: 1597 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2236 4246 5147 5409 820 Size: 197 926 293 2010 901 262 1553 Fragments arranged by size: 2010 1553 926 901 293 262 197 SnaBI TAC'GTA Cuts at: 493 3933 493 Size: 3440 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 2666 3500 1132 Size: 1534 834 3774 Fragments arranged by size: 3774 1534 834 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6142 StyI C'CwwG_G Cuts at: 513 1065 2004 2520 3141 3148 3581 513 Size: 552 939 516 621 7 433 3074 Fragments arranged by size: 3074 939 621 552 516 433 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3291 4169 4186 4339 4524 4969 325 Size: 1255 1711 878 17 153 185 445 1498 Fragments arranged by size: 1711 1498 1255 878 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2408 3228 4388 Size: 274 80 33 51 1443 431 820 1160 Cuts at: 4388 6100 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1160 820 431 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6102 6134 6102 Size: 32 6110 Tth111I GACn'n_nGTC Cuts at: 1316 2107 1316 Size: 791 5351 XbaI T'CTAG_A Cuts at: 3478 3478 Size: 6142 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2492 3357 2492 Size: 865 5277 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 3467 1870 Size: 1597 4545 XmnI GAAnn'nnTTC Cuts at: 4271 4271 Size: 6142 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2467 3950 276 Size: 53 83 186 936 933 1483 2468 Fragments arranged by size: 2468 1483 936 933 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI