Restriction map for
pFN21AB8348
(Linear) (Six-base) MAPSORT of: KIBB8348.seq Check: 2951 from: 1 to: 1437
KIBB8348.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AatII G_ACGT'C
Cuts at: 0 464 1437
Size: 464 973
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 686 734 869 1437
Size: 596 90 48 135 568
Fragments arranged by size:
596 568 135 90 48
AfeI AGC'GCT
Cuts at: 0 1153 1437
Size: 1153 284
AflIII A'CryG_T
Cuts at: 0 147 1437
Size: 147 1290
AgeI A'CCGG_T
Cuts at: 0 781 1437
Size: 781 656
AlwNI CAG_nnn'CTG
Cuts at: 0 426 1437
Size: 426 1011
ApoI r'AATT_y
Cuts at: 0 1359 1437
Size: 1359 78
BamHI G'GATC_C
Cuts at: 0 1026 1437
Size: 1026 411
BanI G'GyrC_C
Cuts at: 0 1000 1293 1437
Size: 1000 293 144
Fragments arranged by size:
1000 293 144
BanII G_rGCy'C
Cuts at: 0 145 761 959 1258 1437
Size: 145 616 198 299 179
Fragments arranged by size:
616 299 198 179 145
BbeI G_GCGC'C
Cuts at: 0 1297 1437
Size: 1297 140
BbsI GAAGACnn'nnnn_
Cuts at: 0 857 1437
Size: 857 580
BbvCI CC'TCA_GC
Cuts at: 0 155 1437
Size: 155 1282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 357 391 1437
Size: 357 34 1046
Fragments arranged by size:
1046 357 34
BfrBI ATG'CAT
Cuts at: 0 657 1437
Size: 657 780
BlpI GC'TnA_GC
Cuts at: 0 1093 1437
Size: 1093 344
Bme1580I G_kGCm'C
Cuts at: 0 842 1429 1437
Size: 842 587 8
Fragments arranged by size:
842 587 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 798 1379 1437
Size: 798 581 58
Fragments arranged by size:
798 581 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 448 963 973 1180 1289 1437
Size: 96 352 515 10 207 109 148
Fragments arranged by size:
515 352 207 148 109 96 10
Bpu10I CC'TnA_GC
Cuts at: 0 155 1437
Size: 155 1282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 1437
Size: 216 1221
BsaBI GATnn'nnATC
Cuts at: 0 875 1437
Size: 875 562
BsaHI Gr'CG_yC
Cuts at: 0 461 1294 1437
Size: 461 833 143
Fragments arranged by size:
833 461 143
BsaWI w'CCGG_w
Cuts at: 0 781 1437
Size: 781 656
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 946 976 1240 1270 1437
Size: 946 30 264 30 167
Fragments arranged by size:
946 264 167 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 99 340 584 1437
Size: 99 241 244 853
Fragments arranged by size:
853 244 241 99
BseYI C'CCAG_C
Cuts at: 0 180 541 1049 1437
Size: 180 361 508 388
Fragments arranged by size:
508 388 361 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 1437
Size: 698 739
BsiHKAI G_wGCw'C
Cuts at: 0 959 1437
Size: 959 478
BsmI GAATG_Cn'
Cuts at: 0 256 1437
Size: 256 1181
Bsp1286I G_dGCh'C
Cuts at: 0 145 761 842 959 1258 1429 1437
Size: 145 616 81 117 299 171 8
Fragments arranged by size:
616 299 171 145 117 81 8
BspHI T'CATG_A
Cuts at: 0 1130 1437
Size: 1130 307
BsrBI CCG'CTC
Cuts at: 0 1059 1437
Size: 1059 378
BsrFI r'CCGG_y
Cuts at: 0 781 1437
Size: 781 656
BsrGI T'GTAC_A
Cuts at: 0 1223 1437
Size: 1223 214
BstEII G'GTnAC_C
Cuts at: 0 189 1437
Size: 189 1248
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 226 1437
Size: 201 25 1211
Fragments arranged by size:
1211 201 25
BstYI r'GATC_y
Cuts at: 0 387 870 929 1026 1437
Size: 387 483 59 97 411
Fragments arranged by size:
483 411 387 97 59
BtgI C'CryG_G
Cuts at: 0 646 1143 1176 1437
Size: 646 497 33 261
Fragments arranged by size:
646 497 261 33
BtsI GCAGTG_nn'
Cuts at: 0 261 427 927 1437
Size: 261 166 500 510
Fragments arranged by size:
510 500 261 166
ClaI AT'CG_AT
Cuts at: 0 488 1437
Size: 488 949
EaeI y'GGCC_r
Cuts at: 0 2 260 789 924 1437
Size: 2 258 529 135 513
Fragments arranged by size:
529 513 258 135 2
EarI CTCTTCn'nnn_
Cuts at: 0 602 1057 1437
Size: 602 455 380
Fragments arranged by size:
602 455 380
EcoICRI GAG'CTC
Cuts at: 0 957 1437
Size: 957 480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 448 596 686 734 869 963 973
Size: 96 352 148 90 48 135 94 10
Cuts at: 973 1180 1289 1437
Size: 207 109 148
Fragments arranged by size:
352 207 148 148 135 109 96 94
90 48 10
EcoO109I rG'GnC_Cy
Cuts at: 0 1191 1437
Size: 1191 246
EcoRI G'AATT_C
Cuts at: 0 1359 1437
Size: 1359 78
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 39 540 1188 1302 1437
Size: 39 501 648 114 135
Fragments arranged by size:
648 501 135 114 39
HaeII r_GCGC'y
Cuts at: 0 1050 1155 1297 1437
Size: 1050 105 142 140
Fragments arranged by size:
1050 142 140 105
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 154 186 946 978 1011 1043
Size: 31 32 91 32 760 32 33 32
Cuts at: 1043 1437
Size: 394
Fragments arranged by size:
760 394 91 33 32 32 32 32
31
HincII GTy'rAC
Cuts at: 0 465 1437
Size: 465 972
KasI G'GCGC_C
Cuts at: 0 1293 1437
Size: 1293 144
MluI A'CGCG_T
Cuts at: 0 147 1437
Size: 147 1290
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 998 1437
Size: 944 54 439
Fragments arranged by size:
944 439 54
MscI TGG'CCA
Cuts at: 0 4 262 926 1437
Size: 4 258 664 511
Fragments arranged by size:
664 511 258 4
MslI CAynn'nnrTG
Cuts at: 0 199 1437
Size: 199 1238
MspA1I CmG'CkG
Cuts at: 0 983 1437
Size: 983 454
NarI GG'CG_CC
Cuts at: 0 1294 1437
Size: 1294 143
NcoI C'CATG_G
Cuts at: 0 1143 1437
Size: 1143 294
NdeI CA'TA_TG
Cuts at: 0 753 1409 1437
Size: 753 656 28
Fragments arranged by size:
753 656 28
NsiI A_TGCA'T
Cuts at: 0 659 1437
Size: 659 778
NspI r_CATG'y
Cuts at: 0 339 661 1437
Size: 339 322 776
Fragments arranged by size:
776 339 322
PflMI CCAn_nnn'nTGG
Cuts at: 0 922 1437
Size: 922 515
PfoI T'CCnGG_A
Cuts at: 0 950 1437
Size: 950 487
PshAI GACnn'nnGTC
Cuts at: 0 857 1138 1437
Size: 857 281 299
Fragments arranged by size:
857 299 281
PvuII CAG'CTG
Cuts at: 0 983 1437
Size: 983 454
SacI G_AGCT'C
Cuts at: 0 959 1437
Size: 959 478
SapI GCTCTTCn'nnn_
Cuts at: 0 1057 1437
Size: 1057 380
SfoI GGC'GCC
Cuts at: 0 1295 1437
Size: 1295 142
SmlI C'TyrA_G
Cuts at: 0 231 1437
Size: 231 1206
SphI G_CATG'C
Cuts at: 0 661 1437
Size: 661 776
StyI C'CwwG_G
Cuts at: 0 515 1136 1143 1437
Size: 515 621 7 294
Fragments arranged by size:
621 515 294 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1286 1437
Size: 1286 151
TatI w'GTAC_w
Cuts at: 0 403 1223 1437
Size: 403 820 214
Fragments arranged by size:
820 403 214
Tth111I GACn'n_nGTC
Cuts at: 0 102 1437
Size: 102 1335
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 487 1352 1437
Size: 487 865 85
Fragments arranged by size:
865 487 85
ZraI GAC'GTC
Cuts at: 0 462 1437
Size: 462 975
Enzymes that do cut:
AatII AcuI AfeI AflIII AgeI AlwNI ApoI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BfrBI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BsrBI BsrFI BsrGI BstEII BstXI BstYI BtgI BtsI
ClaI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI
HaeII Hin4I HincII KasI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NsiI NspI PflMI PfoI
PshAI PvuII SacI SapI SfoI SmlI SphI StyI
TaqII TatI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BciVI BclI BglI BglII BmgBI BmtI BplI
BsaI BsaAI BsiEI BsiWI BsmBI BspEI BspMI BsrDI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI CspCI
DraI DraIII DrdI EagI EciI EcoNI EcoRV FseI
FspI FspAI HindIII HpaI KpnI MfeI NaeI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TstI XbaI XhoI XmaI XmnI