(Circular) (Six-base) MAPSORT of: pFN21AB7851.seq Check: 9871 from: 1 to: 6349 pFN21AB7851 6349 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3394 3394 Size: 6349 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4159 278 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2967 3692 1937 Size: 1030 725 4594 Fragments arranged by size: 4594 1030 725 Acc65I G'GTAC_C Cuts at: 2940 3670 2940 Size: 730 5619 AclI AA'CG_TT Cuts at: 1563 4476 4849 1563 Size: 2913 373 3063 Fragments arranged by size: 3063 2913 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2902 4412 5796 6064 1632 Size: 1270 1510 1384 268 1917 Fragments arranged by size: 1917 1510 1384 1270 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 5248 6225 1203 Size: 4045 977 1327 Fragments arranged by size: 4045 1327 977 AgeI A'CCGG_T Cuts at: 3079 5180 3079 Size: 2101 4248 AhdI GACnn_n'nnGTC Cuts at: 5078 5078 Size: 6349 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6349 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3448 1184 Size: 2264 4085 AlwNI CAG_nnn'CTG Cuts at: 2568 4092 5664 2568 Size: 1524 1572 3253 Fragments arranged by size: 3253 1572 1524 ApaI G_GGCC'C Cuts at: 2257 2664 2886 3503 3576 2257 Size: 407 222 617 73 5030 Fragments arranged by size: 5030 617 407 222 73 ApaLI G'TGCA_C Cuts at: 4406 5562 4406 Size: 1156 5193 ApoI r'AATT_y Cuts at: 1493 2165 3658 3884 4036 6145 1493 Size: 672 1493 226 152 2109 1697 Fragments arranged by size: 2109 1697 1493 672 226 152 AseI AT'TA_AT Cuts at: 160 1032 4903 160 Size: 872 3871 1606 Fragments arranged by size: 3871 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 2718 3068 3419 3674 1870 Size: 73 775 350 351 255 4545 Fragments arranged by size: 4545 775 351 350 255 73 BamHI G'GATC_C Cuts at: 2611 2774 3679 4046 2611 Size: 163 905 367 4914 Fragments arranged by size: 4914 905 367 163 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2648 2940 3019 3064 3415 Size: 325 217 627 861 292 79 45 351 Cuts at: 3415 3670 5125 618 Size: 255 1455 1842 Fragments arranged by size: 1842 1455 861 627 351 325 292 255 217 79 45 BanII G_rGCy'C Cuts at: 1391 2257 2664 2858 2886 3291 3408 3503 3576 Size: 866 407 194 28 405 117 95 73 Cuts at: 3576 3599 3668 1391 Size: 23 69 4072 Fragments arranged by size: 4072 866 407 405 194 117 95 73 69 28 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2015 2689 3357 3480 928 Size: 971 116 674 668 123 3797 Fragments arranged by size: 3797 971 674 668 123 116 BbvCI CC'TCA_GC Cuts at: 2738 2790 2981 3234 2738 Size: 52 191 253 5853 Fragments arranged by size: 5853 253 191 52 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4540 4574 1903 Size: 34 2603 34 3678 Fragments arranged by size: 3678 2603 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4241 5451 4241 Size: 1210 5139 BclI T'GATC_A Cuts at: 1548 1548 Size: 6349 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2547 4960 136 Size: 107 122 71 631 605 875 2413 1525 Fragments arranged by size: 2413 1525 875 631 605 122 107 71 BglII A'GATC_T Cuts at: 6344 6344 Size: 6349 BlpI GC'TnA_GC Cuts at: 2246 2852 3766 2246 Size: 606 914 4829 Fragments arranged by size: 4829 914 606 Bme1580I G_kGCm'C Cuts at: 1790 2257 2391 2401 2664 2886 3024 3050 3382 Size: 467 134 10 263 222 138 26 332 Cuts at: 3382 3503 3576 4410 5566 1790 Size: 121 73 834 1156 2573 Fragments arranged by size: 2573 1156 834 467 332 263 222 138 134 121 73 26 10 BmgBI CAC'GTC Cuts at: 1318 2583 1318 Size: 1265 5084 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2862 3424 4080 Size: 416 53 83 399 24 1445 562 656 Cuts at: 4080 5038 442 Size: 958 1753 Fragments arranged by size: 1753 1445 958 656 562 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3254 1056 Size: 2198 4151 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3310 3310 Size: 6349 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2612 3125 3487 3590 3653 5009 1239 Size: 887 486 513 362 103 63 1356 2579 Fragments arranged by size: 2579 1356 887 513 486 362 103 63 Bpu10I CC'TnA_GC Cuts at: 2738 2790 2893 2981 3234 3396 3402 2738 Size: 52 103 88 253 162 6 5685 Fragments arranged by size: 5685 253 162 103 88 52 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2059 3428 4474 5339 5637 2059 Size: 1369 1046 865 298 2771 Fragments arranged by size: 2771 1369 1046 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3065 5012 882 Size: 617 1566 1947 2219 Fragments arranged by size: 2219 1947 1566 617 BsaAI yAC'GTr Cuts at: 493 1204 4140 493 Size: 711 2936 2702 Fragments arranged by size: 2936 2702 711 BsaBI GATnn'nnATC Cuts at: 6343 6343 Size: 6349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2136 2816 3160 3371 Size: 53 83 186 936 603 680 344 211 Cuts at: 3371 4156 4538 5173 275 Size: 785 382 635 1451 Fragments arranged by size: 1451 936 785 680 635 603 382 344 211 186 83 53 BsaWI w'CCGG_w Cuts at: 2638 3079 4782 5180 5454 5601 6012 2638 Size: 441 1703 398 274 147 411 2975 Fragments arranged by size: 2975 1703 441 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3629 3659 1178 Size: 30 165 30 2226 30 3868 Fragments arranged by size: 3868 2226 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2138 3079 3326 1186 Size: 952 941 247 4209 Fragments arranged by size: 4209 952 941 247 BseYI C'CCAG_C Cuts at: 2375 2602 2885 3024 3030 3176 3506 3566 3621 Size: 227 283 139 6 146 330 60 55 Cuts at: 3621 5552 6048 2375 Size: 1931 496 2676 Fragments arranged by size: 2676 1931 496 330 283 227 146 139 60 55 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2270 2403 2984 3240 3372 1163 Size: 573 534 133 581 256 132 4140 Fragments arranged by size: 4140 581 573 534 256 133 132 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3799 4560 5588 1540 Size: 275 186 1798 761 1028 2301 Fragments arranged by size: 2301 1798 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3599 3639 4410 4495 5566 3599 Size: 40 771 85 1071 4382 Fragments arranged by size: 4382 1071 771 85 40 BsmI GAATG_Cn' Cuts at: 2280 3860 3953 2280 Size: 1580 93 4676 Fragments arranged by size: 4676 1580 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2257 2391 2401 2664 2858 2886 3024 Size: 399 467 134 10 263 194 28 138 Cuts at: 3024 3050 3291 3382 3408 3503 3576 3599 3639 Size: 26 241 91 26 95 73 23 40 Cuts at: 3639 3668 4410 4495 5566 1391 Size: 29 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 467 399 263 241 194 138 134 95 91 85 73 40 29 28 26 26 23 10 BspEI T'CCGG_A Cuts at: 2638 6012 2638 Size: 3374 2975 BspHI T'CATG_A Cuts at: 4238 5968 4238 Size: 1730 4619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2952 3394 3704 844 Size: 2108 442 310 3489 Fragments arranged by size: 3489 2108 442 310 BsrBI CCG'CTC Cuts at: 1394 1823 2484 3210 4236 1394 Size: 429 661 726 1026 3507 Fragments arranged by size: 3507 1026 726 661 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2139 4844 5018 65 Size: 1183 891 2705 174 1396 Fragments arranged by size: 2705 1396 1183 891 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2765 3079 3162 3194 4993 5180 1700 Size: 110 955 314 83 32 1799 187 2869 Fragments arranged by size: 2869 1799 955 314 187 110 83 32 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6349 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6349 BssSI C'ACGA_G Cuts at: 4409 5421 4409 Size: 1012 5337 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6349 BstBI TT'CG_AA Cuts at: 5153 5153 Size: 6349 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6349 BstXI CCAn_nnnn'nTGG Cuts at: 2690 2827 2690 Size: 137 6212 BstYI r'GATC_y Cuts at: 1972 2082 2611 2641 2774 3679 4046 4431 4448 Size: 110 529 30 133 905 367 385 17 Cuts at: 4448 5900 5986 5998 6344 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 905 529 385 367 346 133 110 86 30 17 12 Bsu36I CC'TnA_GG Cuts at: 2925 2925 Size: 6349 BtgI C'CryG_G Cuts at: 513 1065 3060 3615 513 Size: 552 1995 555 3247 Fragments arranged by size: 3247 1995 555 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5610 Fragments arranged by size: 5610 683 56 BtsI GCAGTG_nn' Cuts at: 2391 3223 3865 4659 4679 2391 Size: 832 642 794 20 4061 Fragments arranged by size: 4061 832 794 642 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3193 3228 529 Size: 35 2629 35 3650 Fragments arranged by size: 3650 2629 35 35 DraI TTT'AAA Cuts at: 3653 3653 Size: 6349 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2693 5356 809 Size: 507 1377 2663 1802 Fragments arranged by size: 2663 1802 1377 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2763 3796 4685 Size: 54 1425 50 275 18 933 1033 889 Cuts at: 4685 6314 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1033 933 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3796 1537 Size: 275 1984 4090 Fragments arranged by size: 4090 1984 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2506 2525 3112 4279 1353 Size: 1153 19 587 1167 3423 Fragments arranged by size: 3423 1167 1153 587 19 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2033 4936 5310 5456 387 Size: 1064 138 444 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 444 374 146 138 EcoICRI GAG'CTC Cuts at: 3597 3597 Size: 6349 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2612 2902 3125 3487 3590 3653 Size: 393 494 486 290 223 362 103 63 Cuts at: 3653 4412 5009 5796 6064 1239 Size: 759 597 787 268 1524 Fragments arranged by size: 1524 787 759 597 494 486 393 362 290 268 223 103 63 EcoO109I rG'GnC_Cy Cuts at: 2254 2476 2660 2661 2882 3499 3500 3554 3572 Size: 222 184 1 221 617 1 54 18 Cuts at: 3572 3573 2254 Size: 1 5030 Fragments arranged by size: 5030 617 222 221 184 54 18 1 1 1 EcoRI G'AATT_C Cuts at: 2165 3658 4036 2165 Size: 1493 378 4478 Fragments arranged by size: 4478 1493 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3469 3469 Size: 6349 FspI TGC'GCA Cuts at: 4855 4855 Size: 6349 HaeII r_GCGC'y Cuts at: 1678 3037 3251 3466 5496 1678 Size: 1359 214 215 2030 2531 Fragments arranged by size: 2531 2030 1359 215 214 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2447 2468 2479 2500 2544 Size: 32 135 32 875 21 11 21 44 Cuts at: 2544 2567 2576 2599 4990 5022 5064 5096 1373 Size: 23 9 23 2391 32 42 32 2626 Fragments arranged by size: 2626 2391 875 135 44 42 32 32 32 32 23 23 21 21 11 9 HincII GTy'rAC Cuts at: 669 1657 1938 3693 3939 669 Size: 988 281 1755 246 3079 Fragments arranged by size: 3079 1755 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6349 HpaI GTT'AAC Cuts at: 3939 3939 Size: 6349 KpnI G_GTAC'C Cuts at: 2944 3674 2944 Size: 730 5619 MfeI C'AATT_G Cuts at: 3948 3948 Size: 6349 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3288 5463 5647 1305 Size: 1983 2175 184 2007 Fragments arranged by size: 2175 2007 1983 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4527 Fragments arranged by size: 4527 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 3430 4307 4666 4825 518 Size: 648 1197 1067 877 359 159 2042 Fragments arranged by size: 2042 1197 1067 877 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2602 2668 2889 3052 3169 3176 3506 Size: 483 513 66 221 163 117 7 330 Cuts at: 3506 3765 4442 5590 5835 1606 Size: 259 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 513 483 330 259 245 221 163 117 66 7 NaeI GCC'GGC Cuts at: 3164 3164 Size: 6349 NcoI C'CATG_G Cuts at: 513 1065 3060 513 Size: 552 1995 3802 Fragments arranged by size: 3802 1995 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6349 NgoMIV G'CCGG_C Cuts at: 3162 3162 Size: 6349 NheI G'CTAG_C Cuts at: 1052 3250 1052 Size: 2198 4151 NotI GC'GGCC_GC Cuts at: 3796 3796 Size: 6349 NspI r_CATG'y Cuts at: 1132 1229 3439 3614 3707 5252 1132 Size: 97 2210 175 93 1545 2229 Fragments arranged by size: 2229 2210 1545 175 97 93 PasI CC'CwG_GG Cuts at: 1348 3008 1348 Size: 1660 4689 PciI A'CATG_T Cuts at: 5248 5248 Size: 6349 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6349 PfoI T'CCnGG_A Cuts at: 2037 2103 2770 2037 Size: 66 667 5616 Fragments arranged by size: 5616 667 66 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4418 4450 5931 5963 4418 Size: 32 1481 32 4804 Fragments arranged by size: 4804 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2476 2476 Size: 6349 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6349 PsiI TTA'TAA Cuts at: 3919 3919 Size: 6349 PspOMI G'GGCC_C Cuts at: 2253 2660 2882 3499 3572 2253 Size: 407 222 617 73 5030 Fragments arranged by size: 5030 617 407 222 73 PstI C_TGCA'G Cuts at: 830 2949 3701 830 Size: 2119 752 3478 Fragments arranged by size: 3478 2119 752 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6349 PvuII CAG'CTG Cuts at: 2089 3176 2089 Size: 1087 5262 SacI G_AGCT'C Cuts at: 3599 3599 Size: 6349 SalI G'TCGA_C Cuts at: 1936 3691 1936 Size: 1755 4594 SapI GCTCTTCn'nnn_ Cuts at: 2506 2506 Size: 6349 SbfI CC_TGCA'GG Cuts at: 2949 3701 2949 Size: 752 5597 ScaI AGT'ACT Cuts at: 4597 6309 4597 Size: 1712 4637 SfcI C'TryA_G Cuts at: 826 1045 2569 2945 3697 4832 5513 5704 826 Size: 219 1524 376 752 1135 681 191 1471 Fragments arranged by size: 1524 1471 1135 752 681 376 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6349 SmaI CCC'GGG Cuts at: 1872 3421 3676 1872 Size: 1549 255 4545 Fragments arranged by size: 4545 1549 255 SmlI C'TyrA_G Cuts at: 820 1017 1943 2074 3443 4453 5354 5616 820 Size: 197 926 131 1369 1010 901 262 1553 Fragments arranged by size: 1553 1369 1010 926 901 262 197 131 SnaBI TAC'GTA Cuts at: 493 4140 493 Size: 3647 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6349 SphI G_CATG'C Cuts at: 1132 3439 3614 3707 1132 Size: 2307 175 93 3774 Fragments arranged by size: 3774 2307 175 93 SspI AAT'ATT Cuts at: 5 52 4273 5 Size: 47 4221 2081 Fragments arranged by size: 4221 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6349 StyI C'CwwG_G Cuts at: 513 1065 2295 3060 3584 3788 513 Size: 552 1230 765 524 204 3074 Fragments arranged by size: 3074 1230 765 552 524 204 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2837 2972 4376 4393 4546 4731 5176 Size: 1255 1257 135 1404 17 153 185 445 Cuts at: 5176 325 Size: 1498 Fragments arranged by size: 1498 1404 1257 1255 445 185 153 135 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4595 6307 96 Size: 274 80 33 51 1443 2618 1712 138 Fragments arranged by size: 2618 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3629 3661 6309 6341 3629 Size: 32 2648 32 3637 Fragments arranged by size: 3637 2648 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6349 XbaI T'CTAG_A Cuts at: 3685 3685 Size: 6349 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6349 XmaI C'CCGG_G Cuts at: 1870 3419 3674 1870 Size: 1549 255 4545 Fragments arranged by size: 4545 1549 255 XmnI GAAnn'nnTTC Cuts at: 2306 4478 2306 Size: 2172 4177 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4157 276 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI