Restriction map for
pFN21AE0036
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BclI BfrI BglII BlnI BsaAI
BsaBI BsiWI BsmI BspDI BspHI
BspLU11I BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI DraI
DraIII DrdI Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HindIII HpaI KasI KpnI KspI
MaeII MamI MluI MseI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PflFI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI SacII SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tru9I Tth111I XbaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 325 TCCGGA T^CCGGA
AhdI 211 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1037 GGGCCC GGGCC^C
Asp700I 1914 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 211 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 861 GGCACC G^G[CT][GA]CC
BbsI 1393 GTCTTC GAAGAC(2/6)
BciVI 2108 GTATCC GTATCC(6/5)
BfaI 478 CTAG C^TAG
BglI 727 GCCAGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1429 CTCCAG CTGGAG(16/14)
BpuAI 1393 GTCTTC GAAGAC(2/6)
BseAI 325 TCCGGA T^CCGGA
BsgI 1060 CTGCAC GTGCAG(16/14)
Bsp1407I 1241 TGTACA T^GTACA
BspEI 325 TCCGGA T^CCGGA
BsrDI 572 GCAATG GCAATG(2/0)
BsrGI 1241 TGTACA T^GTACA
BssHII 1096 GCGCGC G^CGCGC
BstAPI 1100 GCATCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 800 AGATCC [GA]^GATC[CT]
CpoI 322 CGGTCCG CG^G[AT]CCG
EagI 1032 CGGCCG C^GGCCG
Eam1105I 211 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1032 CGGCCG C^GGCCG
Eco52I 1032 CGGCCG C^GGCCG
HincII 751 GTTGAC GT[CT]^[GA]AC
HindII 751 GTTGAC GT[CT]^[GA]AC
MaeI 478 CTAG C^TAG
MfeI 153 CAATTG C^AATTG
MflI 800 AGATCC [GA]^GATC[CT]
MroI 325 TCCGGA T^CCGGA
MunI 153 CAATTG C^AATTG
PaeR7I 1880 CTCGAG C^TCGAG
RsrII 322 CGGTCCG CG^G[AT]CCG
SacI 1615 GAGCTC GAGCT^C
SfiI 726 GGCCAGAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1325 CCCGGG CCC^GGG
SmlI 1880 CTCGAG C^T[CT][GA]AG
SspBI 1241 TGTACA T^GTACA
TfiI 1215 GAATC G^A[AT]TC
XhoI 1880 CTCGAG C^TCGAG
XhoII 800 AGATCC [GA]^GATC[CT]
XmaCI 1325 CCCGGG C^CCGGG
XmaI 1325 CCCGGG C^CCGGG
XmnI 1914 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 909 CGCG CG^CG
1097 CGCG
AcyI 928 GACGCC G[GA]^CG[CT]C
1208 GACGCC
AgeI 234 ACCGGT A^CCGGT
2035 ACCGGT
AlwI 801 GATCC GGATC(4/5)
1523 GGATC
Aor51HI 416 AGCGCT AGC^GCT
1016 AGCGCT
BcgI 177 GCAGCGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1474 GCACCCAACTCG
BlpI 1764 GCTTAGC GC^T[ACGT]AGC
1858 GCTGAGC
Bpu1102I 1764 GCTTAGC GC^T[ACGT]AGC
1858 GCTGAGC
BsaHI 928 GACGCC G[GA]^CG[CT]C
1208 GACGCC
BsiEI 648 CGACCG CG[GA][CT]^CG
1032 CGGCCG
BsmBI 1206 GAGACG CGTCTC(1/5)
1934 GAGACG
BsrBI 855 CCGCTC CCGCTC(-3/-3)
1089 CCGCTC
BstUI 909 CGCG CG^CG
1097 CGCG
CelII 1764 GCTTAGC GC^T[ACGT]AGC
1858 GCTGAGC
Eco47III 416 AGCGCT AGC^GCT
1016 AGCGCT
EcoT14I 206 CCATGG C^C[AT][AT]GG
1271 CCATGG
EcoT22I 1 ATGCAT ATGCA^T
277 ATGCAT
FauI 514 CCCGC CCCGC(4/6)
818 CCCGC
HgaI 928 GACGC GACGC(5/10)
1208 GACGC
Hin1I 928 GACGCC G[GA]^CG[CT]C
1208 GACGCC
MvnI 909 CGCG CG^CG
1097 CGCG
NcoI 206 CCATGG C^CATGG
1271 CCATGG
NsiI 1 ATGCAT ATGCA^T
277 ATGCAT
PinAI 234 ACCGGT A^CCGGT
2035 ACCGGT
PleI 718 GAGTC GAGTC(4/5)
890 GAGTC
PpuMI 26 GGGTCCT [GA]G^G[AT]CC[CT]
763 AGGACCT
PshAI 96 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1069 GACCCCTGTC
SapI 165 GAAGAGC GCTCTTC(1/4)
1925 GAAGAGC
StyI 206 CCATGG C^C[AT][AT]GG
1271 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI AspHI AvaI AvaII BalI
BanII BbvI BcnI BmyI BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI