Restriction map for
pFN21AE0036
(Linear) (Six-base) MAPSORT of: KIEE0036.seq Check: 8848 from: 1 to: 2145
KIEE0036.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1108 2145
Size: 1108 1037
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2049 2145
Size: 2049 96
AfeI AGC'GCT
Cuts at: 0 418 1018 2145
Size: 418 600 1127
Fragments arranged by size:
1127 600 418
AgeI A'CCGG_T
Cuts at: 0 234 2035 2145
Size: 234 1801 110
Fragments arranged by size:
1801 234 110
AhdI GACnn_n'nnGTC
Cuts at: 0 216 2145
Size: 216 1929
AleI CACnn'nnGTG
Cuts at: 0 346 2145
Size: 346 1799
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 885 2145
Size: 885 1260
AlwNI CAG_nnn'CTG
Cuts at: 0 392 498 1949 2145
Size: 392 106 1451 196
Fragments arranged by size:
1451 392 196 106
ApaI G_GGCC'C
Cuts at: 0 1041 2145
Size: 1041 1104
ApoI r'AATT_y
Cuts at: 0 1752 1809 2003 2145
Size: 1752 57 194 142
Fragments arranged by size:
1752 194 142 57
AvaI C'yCGr_G
Cuts at: 0 1325 1880 1931 2084 2145
Size: 1325 555 51 153 61
Fragments arranged by size:
1325 555 153 61 51
BanI G'GyrC_C
Cuts at: 0 861 2145
Size: 861 1284
BanII G_rGCy'C
Cuts at: 0 663 1041 1619 1739 1881 1932 2145
Size: 663 378 578 120 142 51 213
Fragments arranged by size:
663 578 378 213 142 120 51
BbsI GAAGACnn'nnnn_
Cuts at: 0 1386 2145
Size: 1386 759
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 166 200 1463 1497 2145
Size: 166 34 1263 34 648
Fragments arranged by size:
1263 648 166 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2119 2145
Size: 2119 26
BfrBI ATG'CAT
Cuts at: 0 3 279 2145
Size: 3 276 1866
Fragments arranged by size:
1866 276 3
BglI GCCn_nnn'nGGC
Cuts at: 0 733 2145
Size: 733 1412
BlpI GC'TnA_GC
Cuts at: 0 1765 1859 2145
Size: 1765 94 286
Fragments arranged by size:
1765 286 94
Bme1580I G_kGCm'C
Cuts at: 0 67 1041 1349 2145
Size: 67 974 308 796
Fragments arranged by size:
974 796 308 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1414 2145
Size: 1414 731
Bpu10I CC'TnA_GC
Cuts at: 0 1996 2145
Size: 1996 149
BsaI GGTCTCn'nnnn_
Cuts at: 0 88 462 960 2145
Size: 88 374 498 1185
Fragments arranged by size:
1185 498 374 88
BsaHI Gr'CG_yC
Cuts at: 0 929 1209 2145
Size: 929 280 936
Fragments arranged by size:
936 929 280
BsaWI w'CCGG_w
Cuts at: 0 234 325 980 2035 2145
Size: 234 91 655 1055 110
Fragments arranged by size:
1055 655 234 110 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 61 91 1453 1483 2145
Size: 61 30 1362 30 662
Fragments arranged by size:
1362 662 61 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 480 1069 1659 2145
Size: 480 589 590 486
Fragments arranged by size:
590 589 486 480
BseYI C'CCAG_C
Cuts at: 0 12 50 72 317 559 773 1742 2145
Size: 12 38 22 245 242 214 969 403
Fragments arranged by size:
969 403 245 242 214 38 22 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 2145
Size: 1045 1100
BsiEI CG_ry'CG
Cuts at: 0 651 1035 2145
Size: 651 384 1110
Fragments arranged by size:
1110 651 384
BsiHKAI G_wGCw'C
Cuts at: 0 399 1184 1619 2145
Size: 399 785 435 526
Fragments arranged by size:
785 526 435 399
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1200 1928 2145
Size: 1200 728 217
Fragments arranged by size:
1200 728 217
Bsp1286I G_dGCh'C
Cuts at: 0 67 399 663 1041 1184 1349 1619 1739
Size: 67 332 264 378 143 165 270 120
Cuts at: 1739 1881 1932 2145
Size: 142 51 213
Fragments arranged by size:
378 332 270 264 213 165 143 142
120 67 51
BspEI T'CCGG_A
Cuts at: 0 325 2145
Size: 325 1820
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 775 1067 1108 2145
Size: 775 292 41 1037
Fragments arranged by size:
1037 775 292 41
BsrBI CCG'CTC
Cuts at: 0 857 1091 2145
Size: 857 234 1054
Fragments arranged by size:
1054 857 234
BsrDI GCAATG_nn'
Cuts at: 0 579 2145
Size: 579 1566
BsrFI r'CCGG_y
Cuts at: 0 234 1187 1625 2035 2145
Size: 234 953 438 410 110
Fragments arranged by size:
953 438 410 234 110
BsrGI T'GTAC_A
Cuts at: 0 1241 2145
Size: 1241 904
BssHII G'CGCG_C
Cuts at: 0 1096 2145
Size: 1096 1049
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1106 2145
Size: 1106 1039
BstXI CCAn_nnnn'nTGG
Cuts at: 0 135 207 388 993 1608 2145
Size: 135 72 181 605 615 537
Fragments arranged by size:
615 605 537 181 135 72
BstYI r'GATC_y
Cuts at: 0 800 2145
Size: 800 1345
BtgI C'CryG_G
Cuts at: 0 206 651 1271 1378 1695 2045 2145
Size: 206 445 620 107 317 350 100
Fragments arranged by size:
620 445 350 317 206 107 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1839 2145
Size: 1839 306
BtsI GCAGTG_nn'
Cuts at: 0 95 682 740 954 2145
Size: 95 587 58 214 1191
Fragments arranged by size:
1191 587 214 95 58
EaeI y'GGCC_r
Cuts at: 0 32 654 725 1004 1032 1193 1385 1416
Size: 32 622 71 279 28 161 192 31
Cuts at: 1416 1568 2145
Size: 152 577
Fragments arranged by size:
622 577 279 192 161 152 71 32
31 28
EagI C'GGCC_G
Cuts at: 0 1032 2145
Size: 1032 1113
EarI CTCTTCn'nnn_
Cuts at: 0 160 296 1898 1920 2145
Size: 160 136 1602 22 225
Fragments arranged by size:
1602 225 160 136 22
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1536 2145
Size: 1536 609
EcoICRI GAG'CTC
Cuts at: 0 1617 2145
Size: 1617 528
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1414 2049 2145
Size: 1414 635 96
Fragments arranged by size:
1414 635 96
EcoO109I rG'GnC_Cy
Cuts at: 0 27 482 764 1038 1560 1888 1992 2145
Size: 27 455 282 274 522 328 104 153
Fragments arranged by size:
522 455 328 282 274 153 104 27
HaeII r_GCGC'y
Cuts at: 0 420 1020 1728 2145
Size: 420 600 708 417
Fragments arranged by size:
708 600 420 417
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 202 234 520 552 1114 1146
Size: 61 32 109 32 286 32 562 32
Cuts at: 1146 2060 2092 2145
Size: 914 32 53
Fragments arranged by size:
914 562 286 109 61 53 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 753 2145
Size: 753 1392
MfeI C'AATT_G
Cuts at: 0 153 2145
Size: 153 1992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 632 671 2145
Size: 512 120 39 1474
Fragments arranged by size:
1474 512 120 39
MscI TGG'CCA
Cuts at: 0 34 1006 1195 1387 1418 1570 2145
Size: 34 972 189 192 31 152 575
Fragments arranged by size:
972 575 192 189 152 34 31
MslI CAynn'nnrTG
Cuts at: 0 346 670 867 1606 2145
Size: 346 324 197 739 539
Fragments arranged by size:
739 539 346 324 197
MspA1I CmG'CkG
Cuts at: 0 180 191 321 366 777 1517 2145
Size: 180 11 130 45 411 740 628
Fragments arranged by size:
740 628 411 180 130 45 11
NcoI C'CATG_G
Cuts at: 0 206 1271 2145
Size: 206 1065 874
Fragments arranged by size:
1065 874 206
NsiI A_TGCA'T
Cuts at: 0 5 281 2145
Size: 5 276 1864
Fragments arranged by size:
1864 276 5
NspI r_CATG'y
Cuts at: 0 283 1489 1680 2145
Size: 283 1206 191 465
Fragments arranged by size:
1206 465 283 191
PasI CC'CwG_GG
Cuts at: 0 82 1341 2145
Size: 82 1259 804
Fragments arranged by size:
1259 804 82
PflMI CCAn_nnn'nTGG
Cuts at: 0 498 702 1791 2145
Size: 498 204 1089 354
Fragments arranged by size:
1089 498 354 204
PfoI T'CCnGG_A
Cuts at: 0 401 1619 2145
Size: 401 1218 526
Fragments arranged by size:
1218 526 401
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1903 1935 2145
Size: 1903 32 210
Fragments arranged by size:
1903 210 32
PpuMI rG'GwC_Cy
Cuts at: 0 27 764 2145
Size: 27 737 1381
Fragments arranged by size:
1381 737 27
PshAI GACnn'nnGTC
Cuts at: 0 100 1073 2145
Size: 100 973 1072
Fragments arranged by size:
1072 973 100
PspOMI G'GGCC_C
Cuts at: 0 1037 2145
Size: 1037 1108
PspXI vC'TCGA_Gb
Cuts at: 0 1880 2145
Size: 1880 265
PstI C_TGCA'G
Cuts at: 0 424 747 2104 2145
Size: 424 323 1357 41
Fragments arranged by size:
1357 424 323 41
PvuII CAG'CTG
Cuts at: 0 366 777 1517 2145
Size: 366 411 740 628
Fragments arranged by size:
740 628 411 366
RsrII CG'GwC_CG
Cuts at: 0 323 2145
Size: 323 1822
SacI G_AGCT'C
Cuts at: 0 1619 2145
Size: 1619 526
SapI GCTCTTCn'nnn_
Cuts at: 0 160 1920 2145
Size: 160 1760 225
Fragments arranged by size:
1760 225 160
SfcI C'TryA_G
Cuts at: 0 420 743 1350 2100 2145
Size: 420 323 607 750 45
Fragments arranged by size:
750 607 420 323 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 733 2145
Size: 733 1412
SmaI CCC'GGG
Cuts at: 0 1327 2145
Size: 1327 818
SmlI C'TyrA_G
Cuts at: 0 1880 2145
Size: 1880 265
SrfI GCCC'GGGC
Cuts at: 0 1327 2145
Size: 1327 818
StyI C'CwwG_G
Cuts at: 0 206 1271 2145
Size: 206 1065 874
Fragments arranged by size:
1065 874 206
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 90 822 1491 2145
Size: 90 732 669 654
Fragments arranged by size:
732 669 654 90
TatI w'GTAC_w
Cuts at: 0 1241 2145
Size: 1241 904
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 135 495 1338 2145
Size: 135 360 843 807
Fragments arranged by size:
843 807 360 135
XhoI C'TCGA_G
Cuts at: 0 1880 2145
Size: 1880 265
XmaI C'CCGG_G
Cuts at: 0 1325 2145
Size: 1325 820
XmnI GAAnn'nnTTC
Cuts at: 0 1918 2145
Size: 1918 227
Enzymes that do cut:
AarI AcuI AfeI AgeI AhdI AleI AlfI AlwNI
ApaI ApoI AvaI BanI BanII BbsI BcgI BciVI
BfrBI BglI BlpI Bme1580I BpmI Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII
MfeI MmeI MscI MslI MspA1I NcoI NsiI NspI
PasI PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuII RsrII SacI SapI SfcI SfiI SmaI
SmlI SrfI StyI TaqII TatI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AloI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BclI BglII BmgBI BmrI BmtI BplI BpuEI BsaAI
BsaBI BsiWI BsmI BspHI BssSI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PciI PmeI PmlI PsiI PsrI PvuI
SacII SalI SanDI SbfI ScaI SexAI SfoI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI TstI
Tth111I XbaI ZraI