(Circular) (Six-base) MAPSORT of: pFN21AB6305.seq Check: 2860 from: 1 to: 5479 pFN21AB6305 5479 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3289 278 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2822 1937 Size: 885 4594 Acc65I G'GTAC_C Cuts at: 2800 2800 Size: 5479 AclI AA'CG_TT Cuts at: 1563 2778 3606 3979 1563 Size: 1215 828 373 3063 Fragments arranged by size: 3063 1215 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3542 4926 5194 1632 Size: 1910 1384 268 1917 Fragments arranged by size: 1917 1910 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5282 AflIII A'CryG_T Cuts at: 1203 4378 5355 1203 Size: 3175 977 1327 Fragments arranged by size: 3175 1327 977 AgeI A'CCGG_T Cuts at: 2118 4310 2118 Size: 2192 3287 AhdI GACnn_n'nnGTC Cuts at: 4208 4208 Size: 5479 AleI CACnn'nnGTG Cuts at: 1166 2145 2573 1166 Size: 979 428 4072 Fragments arranged by size: 4072 979 428 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5479 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2580 2612 2580 Size: 32 5447 AlwNI CAG_nnn'CTG Cuts at: 2436 2491 3222 4794 2436 Size: 55 731 1572 3121 Fragments arranged by size: 3121 1572 731 55 ApaI G_GGCC'C Cuts at: 2630 2630 Size: 5479 ApaLI G'TGCA_C Cuts at: 3536 4692 3536 Size: 1156 4323 ApoI r'AATT_y Cuts at: 1493 2086 2788 3014 3166 5275 1493 Size: 593 702 226 152 2109 1697 Fragments arranged by size: 2109 1697 702 593 226 152 AseI AT'TA_AT Cuts at: 160 1032 4033 160 Size: 872 3001 1606 Fragments arranged by size: 3001 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 AvaI C'yCGr_G Cuts at: 1870 1943 2804 1870 Size: 73 861 4545 Fragments arranged by size: 4545 861 73 BamHI G'GATC_C Cuts at: 2809 3176 2809 Size: 367 5112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2800 4255 618 Size: 325 217 627 1013 1455 1842 Fragments arranged by size: 1842 1455 1013 627 325 217 BanII G_rGCy'C Cuts at: 1391 2555 2630 2798 1391 Size: 1164 75 168 4072 Fragments arranged by size: 4072 1164 168 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2216 2716 928 Size: 971 317 500 3691 Fragments arranged by size: 3691 971 500 317 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3670 3704 1903 Size: 34 50 34 1649 34 3678 Fragments arranged by size: 3678 1649 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3371 4581 3371 Size: 1210 4269 BclI T'GATC_A Cuts at: 1548 1548 Size: 5479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2420 4090 136 Size: 107 122 71 631 605 748 1670 1525 Fragments arranged by size: 1670 1525 748 631 605 122 107 71 BglII A'GATC_T Cuts at: 5474 5474 Size: 5479 BlpI GC'TnA_GC Cuts at: 2896 2896 Size: 5479 Bme1580I G_kGCm'C Cuts at: 1790 2630 3540 4696 1790 Size: 840 910 1156 2573 Fragments arranged by size: 2573 1156 910 840 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5479 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2182 2557 3210 Size: 416 53 83 399 24 765 375 653 Cuts at: 3210 4168 442 Size: 958 1753 Fragments arranged by size: 1753 958 765 653 416 399 375 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5479 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2096 2145 2250 2518 2692 4139 1239 Size: 857 49 105 268 174 1447 2579 Fragments arranged by size: 2579 1447 857 268 174 105 49 Bpu10I CC'TnA_GC Cuts at: 2243 2243 Size: 5479 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3604 4469 4767 3604 Size: 865 298 4316 Fragments arranged by size: 4316 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2226 2740 4142 882 Size: 617 727 514 1402 2219 Fragments arranged by size: 2219 1402 727 617 514 BsaAI yAC'GTr Cuts at: 493 1204 2272 3270 493 Size: 711 1068 998 2702 Fragments arranged by size: 2702 1068 998 711 BsaBI GATnn'nnATC Cuts at: 5473 5473 Size: 5479 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3286 3668 4303 275 Size: 53 83 186 936 1753 382 635 1451 Fragments arranged by size: 1753 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2041 2118 3912 4310 4584 4731 5142 2041 Size: 77 1794 398 274 147 411 2378 Fragments arranged by size: 2378 1794 411 398 274 147 77 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2537 2567 2580 2610 1178 Size: 30 165 30 1134 30 13 30 4047 Fragments arranged by size: 4047 1134 165 30 30 30 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2530 1186 Size: 1344 4135 BseYI C'CCAG_C Cuts at: 2389 2451 4682 5178 2389 Size: 62 2231 496 2690 Fragments arranged by size: 2690 2231 496 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 1163 Size: 573 564 4342 Fragments arranged by size: 4342 573 564 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2929 3690 4718 1540 Size: 275 186 928 761 1028 2301 Fragments arranged by size: 2301 1028 928 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3540 3625 4696 3540 Size: 85 1071 4323 Fragments arranged by size: 4323 1071 85 BsmI GAATG_Cn' Cuts at: 2990 3083 2990 Size: 93 5386 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2555 2630 2798 3540 3625 4696 1391 Size: 399 765 75 168 742 85 1071 2174 Fragments arranged by size: 2174 1071 765 742 399 168 85 75 BspEI T'CCGG_A Cuts at: 2041 5142 2041 Size: 3101 2378 BspHI T'CATG_A Cuts at: 3368 5098 3368 Size: 1730 3749 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2514 2834 844 Size: 1670 320 3489 Fragments arranged by size: 3489 1670 320 BsrBI CCG'CTC Cuts at: 1394 1823 3366 1394 Size: 429 1543 3507 Fragments arranged by size: 3507 1543 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2635 3974 4148 65 Size: 1183 1387 1339 174 1396 Fragments arranged by size: 1396 1387 1339 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2118 2327 4123 4310 1700 Size: 110 308 209 1796 187 2869 Fragments arranged by size: 2869 1796 308 209 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5479 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5479 BssSI C'ACGA_G Cuts at: 3539 4551 3539 Size: 1012 4467 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5479 BstBI TT'CG_AA Cuts at: 4283 4283 Size: 5479 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5479 BstXI CCAn_nnnn'nTGG Cuts at: 2255 2255 Size: 5479 BstYI r'GATC_y Cuts at: 1972 2809 3176 3561 3578 5030 5116 5128 5474 Size: 837 367 385 17 1452 86 12 346 Cuts at: 5474 1972 Size: 1977 Fragments arranged by size: 1977 1452 837 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2571 2665 513 Size: 552 1506 94 3327 Fragments arranged by size: 3327 1506 552 94 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2323 2752 487 Size: 683 56 1097 429 3214 Fragments arranged by size: 3214 1097 683 429 56 BtsI GCAGTG_nn' Cuts at: 2191 2995 3789 3809 2191 Size: 804 794 20 3861 Fragments arranged by size: 3861 804 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5479 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5444 DraI TTT'AAA Cuts at: 2783 2783 Size: 5479 DraIII CAC_nnn'GTG Cuts at: 2197 2197 Size: 5479 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4486 809 Size: 507 3170 1802 Fragments arranged by size: 3170 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2926 3815 5444 Size: 54 1425 50 275 18 1096 889 1629 Cuts at: 5444 8 Size: 43 Fragments arranged by size: 1629 1425 1096 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2926 1537 Size: 275 1114 4090 Fragments arranged by size: 4090 1114 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3409 1353 Size: 2056 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4066 4440 4586 387 Size: 1064 138 2477 374 146 1280 Fragments arranged by size: 2477 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2145 2250 2518 2692 3542 4139 Size: 393 464 49 105 268 174 850 597 Cuts at: 4139 4926 5194 1239 Size: 787 268 1524 Fragments arranged by size: 1524 850 787 597 464 393 268 268 174 105 49 EcoNI CCTnn'n_nnAGG Cuts at: 2495 2495 Size: 5479 EcoO109I rG'GnC_Cy Cuts at: 2035 2154 2626 2627 2035 Size: 119 472 1 4887 Fragments arranged by size: 4887 472 119 1 EcoRI G'AATT_C Cuts at: 2086 2788 3166 2086 Size: 702 378 4399 Fragments arranged by size: 4399 702 378 FspI TGC'GCA Cuts at: 3985 3985 Size: 5479 HaeII r_GCGC'y Cuts at: 1678 4626 1678 Size: 2948 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2173 2205 4120 4152 4194 Size: 32 135 32 601 32 1915 32 42 Cuts at: 4194 4226 1373 Size: 32 2626 Fragments arranged by size: 2626 1915 601 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2823 3069 669 Size: 988 281 885 246 3079 Fragments arranged by size: 3079 988 885 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5479 HpaI GTT'AAC Cuts at: 3069 3069 Size: 5479 KpnI G_GTAC'C Cuts at: 2804 2804 Size: 5479 MfeI C'AATT_G Cuts at: 3078 3078 Size: 5479 MluI A'CGCG_T Cuts at: 5355 5355 Size: 5479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2044 2799 4593 4777 1305 Size: 739 755 1794 184 2007 Fragments arranged by size: 2007 1794 755 739 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3657 Fragments arranged by size: 3657 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2145 2573 3437 3796 3955 518 Size: 648 979 428 864 359 159 2042 Fragments arranged by size: 2042 979 864 648 428 359 159 MspA1I CmG'CkG Cuts at: 1606 2186 2389 2479 2895 3572 4720 4965 1606 Size: 580 203 90 416 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 580 416 245 203 90 NaeI GCC'GGC Cuts at: 2329 2329 Size: 5479 NcoI C'CATG_G Cuts at: 513 1065 2571 2665 513 Size: 552 1506 94 3327 Fragments arranged by size: 3327 1506 552 94 NdeI CA'TA_TG Cuts at: 387 387 Size: 5479 NgoMIV G'CCGG_C Cuts at: 2327 2327 Size: 5479 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5479 NotI GC'GGCC_GC Cuts at: 2926 2926 Size: 5479 NspI r_CATG'y Cuts at: 1132 1229 2837 4382 1132 Size: 97 1608 1545 2229 Fragments arranged by size: 2229 1608 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5479 PciI A'CATG_T Cuts at: 4378 4378 Size: 5479 PflMI CCAn_nnn'nTGG Cuts at: 1108 2641 1108 Size: 1533 3946 PfoI T'CCnGG_A Cuts at: 2708 2750 2708 Size: 42 5437 PmeI GTTT'AAAC Cuts at: 2783 2783 Size: 5479 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2580 2612 3548 3580 5061 5093 2580 Size: 32 936 32 1481 32 2966 Fragments arranged by size: 2966 1481 936 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2035 2154 2035 Size: 119 5360 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5479 PsiI TTA'TAA Cuts at: 3049 3049 Size: 5479 PspOMI G'GGCC_C Cuts at: 2626 2626 Size: 5479 PstI C_TGCA'G Cuts at: 830 2496 2831 830 Size: 1666 335 3478 Fragments arranged by size: 3478 1666 335 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5479 PvuII CAG'CTG Cuts at: 2186 2389 2479 2186 Size: 203 90 5186 Fragments arranged by size: 5186 203 90 SalI G'TCGA_C Cuts at: 1936 2821 1936 Size: 885 4594 SanDI GG'GwC_CC Cuts at: 2154 2154 Size: 5479 SbfI CC_TGCA'GG Cuts at: 2831 2831 Size: 5479 ScaI AGT'ACT Cuts at: 3727 5439 3727 Size: 1712 3767 SfcI C'TryA_G Cuts at: 826 1045 2492 2718 2827 3962 4643 4834 826 Size: 219 1447 226 109 1135 681 191 1471 Fragments arranged by size: 1471 1447 1135 681 226 219 191 109 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5479 SmaI CCC'GGG Cuts at: 1872 2806 1872 Size: 934 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3583 4484 4746 820 Size: 197 926 1640 901 262 1553 Fragments arranged by size: 1640 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3270 493 Size: 2777 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5479 SphI G_CATG'C Cuts at: 1132 2837 1132 Size: 1705 3774 SspI AAT'ATT Cuts at: 5 52 3403 5 Size: 47 3351 2081 Fragments arranged by size: 3351 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5479 StyI C'CwwG_G Cuts at: 513 1065 2571 2665 2918 513 Size: 552 1506 94 253 3074 Fragments arranged by size: 3074 1506 552 253 94 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2045 2164 2381 2744 3506 3523 3676 Size: 1255 465 119 217 363 762 17 153 Cuts at: 3676 3861 4306 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 762 465 445 363 217 185 153 119 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3725 5437 96 Size: 274 80 33 51 1443 1748 1712 138 Fragments arranged by size: 1748 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5439 5471 5439 Size: 32 5447 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5479 XbaI T'CTAG_A Cuts at: 2815 2815 Size: 5479 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2120 2476 2618 2120 Size: 356 142 4981 Fragments arranged by size: 4981 356 142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5479 XmaI C'CCGG_G Cuts at: 1870 2804 1870 Size: 934 4545 XmnI GAAnn'nnTTC Cuts at: 2213 3608 2213 Size: 1395 4084 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3287 276 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI