################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI Aor51HI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BanI BbeI BclI BfaI BfrI BglII BlnI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI DsaI Eco47III EcoO65I EcoRV EcoT22I FbaI FspI HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MroI MseI MslI MunI NarI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SfuI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 562 GACGTC GACGT^C AccI 565 GTCTAC GT^[AC][GT]AC AcsI 1093 GAATTC [GA]^AATT[CT] AfaI 369 GTAC GT^AC Alw44I 1509 GTGCAC G^TGCAC AlwNI 210 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1509 GTGCAC G^TGCAC ApoI 1093 GAATTC [GA]^AATT[CT] AspI 1537 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1557 TGGCCA TGG^CCA BcgI 443 GCAACATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1313 GTATCC GTATCC(6/5) BlpI 1392 GCTCAGC GC^T[ACGT]AGC BpmI 297 CTGGAG CTGGAG(16/14) Bpu1102I 1392 GCTCAGC GC^T[ACGT]AGC BsaBI 1275 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 955 GAGACC GGTCTC(1/5) BseRI 424 GAGGAG GAGGAG(10/8) BsmAI 955 GAGAC GTCTC(1/5) BsmI 454 GCATTC GAATGC(1/-1) BstAPI 1511 GCACCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1392 GCTCAGC GC^T[ACGT]AGC EcoNI 1593 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1093 GAATTC G^AATTC FseI 1046 GGCCGGCC GGCCGG^CC HaeII 414 GGCGCT [GA]GCGC^[CT] HincII 1156 GTCAAC GT[CT]^[GA]AC HindII 1156 GTCAAC GT[CT]^[GA]AC MamI 1275 GATCCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1557 TGGCCA TGG^CCA MscI 1557 TGGCCA TGG^CCA PflFI 1537 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 332 AGGACCC [GA]G^G[AT]CC[CT] RsaI 369 GTAC GT^AC SexAI 383 ACCTGGT A^CC[AT]GGT SfiI 741 GGCCGCGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 849 CGCCGGCG C[GA]^CCGG[CT]G SmaI 1117 CCCGGG CCC^GGG SmlI 1594 CTCAAG C^T[CT][GA]AG SphI 1205 GCATGC GCATG^C Tth111I 1537 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1117 CCCGGG C^CCGGG XmaI 1117 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AflIII 1552 ACATGT A^C[GA][CT]GT 1562 ACATGT AhdI 382 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1479 GACCCCAGGTC AlwI 168 GATCC GGATC(4/5) 1275 GATCC AocI 421 CCTGAGG CC^T[ACGT]AGG 1069 CCTGAGG AspEI 382 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1479 GACCCCAGGTC BbrPI 1023 CACGTG CAC^GTG 1398 CACGTG BbsI 481 GTCTTC GAAGAC(2/6) 925 GTCTTC BbvCI 180 CCTCAGC CCTCAGC(-5/-2) 1179 CCTCAGC BglI 742 GCCGCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1184 GCCACAATGGC BpuAI 481 GTCTTC GAAGAC(2/6) 925 GTCTTC BsaAI 1023 CACGTG [CT]AC^GT[GA] 1398 CACGTG BspLU11I 1552 ACATGT A^CATGT 1562 ACATGT BspMI 827 ACCTGC ACCTGC(4/8) 1055 ACCTGC BstXI 1292 CCACTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1358 CCAGTGCCCTGG BstYI 167 AGATCC [GA]^GATC[CT] 1274 AGATCC Bsu36I 421 CCTGAGG CC^T[ACGT]AGG 1069 CCTGAGG DraIII 1020 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG 1528 CACGCGGTG Eam1105I 382 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1479 GACCCCAGGTC EarI 139 CTCTTC CTCTTC(1/4) 1246 CTCTTC Eco81I 421 CCTGAGG CC^T[ACGT]AGG 1069 CCTGAGG EcoT14I 155 CCAAGG C^C[AT][AT]GG 410 CCAAGG Ksp632I 139 CTCTTC CTCTTC(1/4) 1246 CTCTTC MflI 167 AGATCC [GA]^GATC[CT] 1274 AGATCC NotI 627 GCGGCCGC GC^GGCCGC 1195 GCGGCCGC PmaCI 1023 CACGTG CAC^GTG 1398 CACGTG PmlI 1023 CACGTG CAC^GTG 1398 CACGTG PvuII 589 CAGCTG CAG^CTG 1210 CAGCTG SapI 138 GCTCTTC GCTCTTC(1/4) 1245 GCTCTTC StyI 155 CCAAGG C^C[AT][AT]GG 410 CCAAGG TaqI 818 TCGA T^CGA 872 TCGA Tsp509I 1094 AATT ^AATT 1447 AATT TthHB8I 818 TCGA T^CGA 872 TCGA XhoII 167 AGATCC [GA]^GATC[CT] 1274 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AspHI AvaI AvaII BanII BbvI BcnI BmyI BsaHI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI