(Linear) (Six-base) MAPSORT of: KIBB5056.seq Check: 8342 from: 1 to: 1671 KIBB5056.orf With 182 enzymes: SgfI * March 2, 2010 14:25 .. AatII G_ACGT'C Cuts at: 0 566 1671 Size: 566 1105 AccI GT'mk_AC Cuts at: 0 566 1671 Size: 566 1105 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 216 292 504 1047 1671 Size: 216 76 212 543 624 Fragments arranged by size: 624 543 216 212 76 AflIII A'CryG_T Cuts at: 0 1552 1562 1671 Size: 1552 10 109 Fragments arranged by size: 1552 109 10 AhdI GACnn_n'nnGTC Cuts at: 0 387 1484 1671 Size: 387 1097 187 Fragments arranged by size: 1097 387 187 AlwNI CAG_nnn'CTG Cuts at: 0 215 1671 Size: 215 1456 ApaI G_GGCC'C Cuts at: 0 49 309 555 1124 1671 Size: 49 260 246 569 547 Fragments arranged by size: 569 547 260 246 49 ApaLI G'TGCA_C Cuts at: 0 1509 1671 Size: 1509 162 ApoI r'AATT_y Cuts at: 0 1093 1671 Size: 1093 578 AvaI C'yCGr_G Cuts at: 0 981 1117 1465 1671 Size: 981 136 348 206 Fragments arranged by size: 981 348 206 136 BanII G_rGCy'C Cuts at: 0 49 122 163 309 555 1124 1671 Size: 49 73 41 146 246 569 547 Fragments arranged by size: 569 547 246 146 73 49 41 BbsI GAAGACnn'nnnn_ Cuts at: 0 474 918 1671 Size: 474 444 753 Fragments arranged by size: 753 474 444 BbvCI CC'TCA_GC Cuts at: 0 181 1180 1671 Size: 181 999 491 Fragments arranged by size: 999 491 181 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 432 466 1671 Size: 432 34 1205 Fragments arranged by size: 1205 432 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1324 1671 Size: 1324 347 BglI GCCn_nnn'nGGC Cuts at: 0 748 1190 1671 Size: 748 442 481 Fragments arranged by size: 748 481 442 BlpI GC'TnA_GC Cuts at: 0 1393 1671 Size: 1393 278 Bme1580I G_kGCm'C Cuts at: 0 49 309 555 1118 1124 1365 1513 1671 Size: 49 260 246 563 6 241 148 158 Fragments arranged by size: 563 260 246 241 158 148 49 6 BmgBI CAC'GTC Cuts at: 0 861 1671 Size: 861 810 BmrI ACTGGGnnnn_n' Cuts at: 0 1352 1671 Size: 1352 319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 318 1671 Size: 318 1353 Bpu10I CC'TnA_GC Cuts at: 0 181 1180 1671 Size: 181 999 491 Fragments arranged by size: 999 491 181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1579 1671 Size: 1579 92 BsaI GGTCTCn'nnnn_ Cuts at: 0 949 1671 Size: 949 722 BsaAI yAC'GTr Cuts at: 0 1025 1400 1671 Size: 1025 375 271 Fragments arranged by size: 1025 375 271 BsaBI GATnn'nnATC Cuts at: 0 1279 1671 Size: 1279 392 BsaHI Gr'CG_yC Cuts at: 0 563 584 1016 1223 1671 Size: 563 21 432 207 448 Fragments arranged by size: 563 448 432 207 21 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1651 1671 Size: 1651 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 439 1671 Size: 439 1232 BseYI C'CCAG_C Cuts at: 0 109 123 315 1671 Size: 109 14 192 1356 Fragments arranged by size: 1356 192 109 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1111 1159 1240 1422 1591 1671 Size: 1111 48 81 182 169 80 Fragments arranged by size: 1111 182 169 81 80 48 BsiEI CG_ry'CG Cuts at: 0 539 631 658 752 1048 1052 1199 1671 Size: 539 92 27 94 296 4 147 472 Fragments arranged by size: 539 472 296 147 94 92 27 4 BsiHKAI G_wGCw'C Cuts at: 0 25 614 902 1513 1671 Size: 25 589 288 611 158 Fragments arranged by size: 611 589 288 158 25 BsmI GAATG_Cn' Cuts at: 0 454 1671 Size: 454 1217 Bsp1286I G_dGCh'C Cuts at: 0 25 49 122 163 309 555 614 902 Size: 25 24 73 41 146 246 59 288 Cuts at: 902 1118 1124 1365 1513 1671 Size: 216 6 241 148 158 Fragments arranged by size: 288 246 241 216 158 148 146 73 59 41 25 24 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 836 1064 1671 Size: 836 228 607 Fragments arranged by size: 836 607 228 BsrBI CCG'CTC Cuts at: 0 302 546 1308 1671 Size: 302 244 762 363 Fragments arranged by size: 762 363 302 244 BsrFI r'CCGG_y Cuts at: 0 747 754 807 850 935 1047 1201 1671 Size: 747 7 53 43 85 112 154 470 Fragments arranged by size: 747 470 154 112 85 53 43 7 BssHII G'CGCG_C Cuts at: 0 685 800 846 854 889 915 917 989 Size: 685 115 46 8 35 26 2 72 Cuts at: 989 1001 1671 Size: 12 670 Fragments arranged by size: 685 670 115 72 46 35 26 12 8 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 1517 1671 Size: 1517 154 BstXI CCAn_nnnn'nTGG Cuts at: 0 1299 1365 1671 Size: 1299 66 306 Fragments arranged by size: 1299 306 66 BstYI r'GATC_y Cuts at: 0 167 1274 1671 Size: 167 1107 397 Fragments arranged by size: 1107 397 167 Bsu36I CC'TnA_GG Cuts at: 0 422 1070 1671 Size: 422 648 601 Fragments arranged by size: 648 601 422 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 435 1517 1671 Size: 435 1082 154 Fragments arranged by size: 1082 435 154 BtsI GCAGTG_nn' Cuts at: 0 107 295 1671 Size: 107 188 1376 Fragments arranged by size: 1376 188 107 DraIII CAC_nnn'GTG Cuts at: 0 1025 1533 1671 Size: 1025 508 138 Fragments arranged by size: 1025 508 138 EaeI y'GGCC_r Cuts at: 0 536 628 655 749 950 1045 1049 1196 Size: 536 92 27 94 201 95 4 147 Cuts at: 1196 1301 1557 1671 Size: 105 256 114 Fragments arranged by size: 536 256 201 147 114 105 95 94 92 27 4 EagI C'GGCC_G Cuts at: 0 536 628 655 749 1045 1049 1196 1671 Size: 536 92 27 94 296 4 147 475 Fragments arranged by size: 536 475 296 147 94 92 27 4 EarI CTCTTCn'nnn_ Cuts at: 0 145 1252 1671 Size: 145 1107 419 Fragments arranged by size: 1107 419 145 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 691 1671 Size: 691 980 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 216 292 318 504 1047 1671 Size: 216 76 26 186 543 624 Fragments arranged by size: 624 543 216 186 76 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 1597 1671 Size: 1597 74 EcoO109I rG'GnC_Cy Cuts at: 0 45 46 305 306 333 552 1074 1121 Size: 45 1 259 1 27 219 522 47 Cuts at: 1121 1671 Size: 550 Fragments arranged by size: 550 522 259 219 47 45 27 1 1 EcoRI G'AATT_C Cuts at: 0 1093 1671 Size: 1093 578 FseI GG_CCGG'CC Cuts at: 0 1051 1671 Size: 1051 620 HaeII r_GCGC'y Cuts at: 0 418 1671 Size: 418 1253 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 373 405 1470 1502 1671 Size: 373 32 1065 32 169 Fragments arranged by size: 1065 373 169 32 32 HincII GTy'rAC Cuts at: 0 1158 1671 Size: 1158 513 MscI TGG'CCA Cuts at: 0 1559 1671 Size: 1559 112 MspA1I CmG'CkG Cuts at: 0 113 135 524 591 1133 1212 1671 Size: 113 22 389 67 542 79 459 Fragments arranged by size: 542 459 389 113 79 67 22 NaeI GCC'GGC Cuts at: 0 749 756 809 852 937 1049 1203 1671 Size: 749 7 53 43 85 112 154 468 Fragments arranged by size: 749 468 154 112 85 53 43 7 NgoMIV G'CCGG_C Cuts at: 0 747 754 807 850 935 1047 1201 1671 Size: 747 7 53 43 85 112 154 470 Fragments arranged by size: 747 470 154 112 85 53 43 7 NotI GC'GGCC_GC Cuts at: 0 628 1196 1671 Size: 628 568 475 Fragments arranged by size: 628 568 475 NspI r_CATG'y Cuts at: 0 1209 1556 1566 1671 Size: 1209 347 10 105 Fragments arranged by size: 1209 347 105 10 PasI CC'CwG_GG Cuts at: 0 1365 1671 Size: 1365 306 PciI A'CATG_T Cuts at: 0 1552 1562 1671 Size: 1552 10 109 Fragments arranged by size: 1552 109 10 PfoI T'CCnGG_A Cuts at: 0 614 1671 Size: 614 1057 PmlI CAC'GTG Cuts at: 0 1025 1400 1671 Size: 1025 375 271 Fragments arranged by size: 1025 375 271 PpuMI rG'GwC_Cy Cuts at: 0 333 1671 Size: 333 1338 PspOMI G'GGCC_C Cuts at: 0 45 305 551 1120 1671 Size: 45 260 246 569 551 Fragments arranged by size: 569 551 260 246 45 PvuII CAG'CTG Cuts at: 0 591 1212 1671 Size: 591 621 459 Fragments arranged by size: 621 591 459 SapI GCTCTTCn'nnn_ Cuts at: 0 145 1252 1671 Size: 145 1107 419 Fragments arranged by size: 1107 419 145 SexAI A'CCwGG_T Cuts at: 0 383 1671 Size: 383 1288 SfiI GGCCn_nnn'nGGCC Cuts at: 0 748 1671 Size: 748 923 SgrAI Cr'CCGG_yG Cuts at: 0 850 1671 Size: 850 821 SmaI CCC'GGG Cuts at: 0 1119 1671 Size: 1119 552 SmlI C'TyrA_G Cuts at: 0 1594 1671 Size: 1594 77 SphI G_CATG'C Cuts at: 0 1209 1671 Size: 1209 462 SrfI GCCC'GGGC Cuts at: 0 1119 1671 Size: 1119 552 StyI C'CwwG_G Cuts at: 0 155 410 1671 Size: 155 255 1261 Fragments arranged by size: 1261 255 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 450 1671 Size: 450 1221 Tth111I GACn'n_nGTC Cuts at: 0 1540 1671 Size: 1540 131 XmaI C'CCGG_G Cuts at: 0 1117 1671 Size: 1117 554 ZraI GAC'GTC Cuts at: 0 564 1671 Size: 564 1107 Enzymes that do cut: AatII AccI AcuI AflIII AhdI AlwNI ApaI ApaLI ApoI AvaI BanII BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgZI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI HaeII Hin4I HincII MscI MspA1I NaeI NgoMIV NotI NspI PasI PciI PfoI PmlI PpuMI PspOMI PvuII SapI SexAI SfiI SgrAI SmaI SmlI SphI SrfI StyI TaqII Tth111I XmaI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AgeI AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BclI BfrBI BglII BmtI BplI BsaWI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstZ17I BtgI ClaI CspCI DraI DrdI EcoICRI EcoRV FalI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NarI NcoI NdeI NheI NruI NsiI PacI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SnaBI SpeI SspI StuI SwaI TatI TstI XbaI XcmI XhoI XmnI