################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## Acc65I AccI AclI AflII AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BciVI BclI BfrI BlnI BsaBI BsiWI Bsp1407I BspHI BspLU11I BsrBI BsrGI Bst1107I BstBI BstZ17I CpoI DraI DraIII DrdI EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MamI MfeI MluI MunI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AccIII 3431 TCCGGA T^CCGGA AcsI 3013 GAATTT [GA]^AATT[CT] Aor51HI 2678 AGCGCT AGC^GCT ApaI 1819 GGGCCC GGGCC^C ApoI 3013 GAATTT [GA]^AATT[CT] Asp700I 2503 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 317 GGATCC G^GATCC BbvCI 3223 GCTGAGG CCTCAGC(-5/-2) BcgI 3537 CGAAGCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BseAI 3431 TCCGGA T^CCGGA BsmI 2514 GAATGC GAATGC(1/-1) BspDI 2890 ATCGAT AT^CGAT BspEI 3431 TCCGGA T^CCGGA BssHII 2518 GCGCGC G^CGCGC ClaI 2890 ATCGAT AT^CGAT Eco47III 2678 AGCGCT AGC^GCT KspI 1093 CCGCGG CCGC^GG MroI 3431 TCCGGA T^CCGGA MseI 3017 TTAA T^TAA NotI 31 GCGGCCGC GC^GGCCGC PshAI 805 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 2892 CGATCG CGAT^CG SacI 3601 GAGCTC GAGCT^C SacII 1093 CCGCGG CCGC^GG SspI 3491 AATATT AAT^ATT StuI 1562 AGGCCT AGG^CCT Tru9I 3017 TTAA T^TAA Tsp509I 3014 AATT ^AATT XmnI 2503 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AflIII 2687 ACGTGT A^C[GA][CT]GT 2923 ACGTGT AhdI 763 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 856 GACAGCAAGTC AocI 1133 CCTCAGG CC^T[ACGT]AGG 2839 CCTGAGG AspEI 763 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 856 GACAGCAAGTC BalI 671 TGGCCA TGG^CCA 1340 TGGCCA BbeI 1775 GGCGCC GGCGC^C 2422 GGCGCC BbrPI 2615 CACGTG CAC^GTG 2922 CACGTG BbsI 1591 GTCTTC GAAGAC(2/6) 3370 GTCTTC BglII 666 AGATCT A^GATCT 2096 AGATCT BlpI 2310 GCTGAGC GC^T[ACGT]AGC 2967 GCTCAGC Bpu1102I 2310 GCTGAGC GC^T[ACGT]AGC 2967 GCTCAGC BpuAI 1591 GTCTTC GAAGAC(2/6) 3370 GTCTTC BsaAI 2615 CACGTG [CT]AC^GT[GA] 2922 CACGTG BsaI 1399 GAGACC GGTCTC(1/5) 1444 GAGACC BsaWI 2975 TCCGGT [AT]^CCGG[AT] 3431 TCCGGA BsrDI 3543 CATTGC GCAATG(2/0) 1376 GCAATG BssSI 513 CACGAG CACGAG(-5/-1) 1109 CACGAG BstAPI 1681 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2972 GCATCCGGTGC Bsu36I 1133 CCTCAGG CC^T[ACGT]AGG 2839 CCTGAGG CelII 2310 GCTGAGC GC^T[ACGT]AGC 2967 GCTCAGC EagI 32 CGGCCG C^GGCCG 1670 CGGCCG Eam1105I 763 GACCCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 856 GACAGCAAGTC EclXI 32 CGGCCG C^GGCCG 1670 CGGCCG Eco52I 32 CGGCCG C^GGCCG 1670 CGGCCG Eco81I 1133 CCTCAGG CC^T[ACGT]AGG 2839 CCTGAGG KasI 1775 GGCGCC G^GCGCC 2422 GGCGCC MluNI 671 TGGCCA TGG^CCA 1340 TGGCCA MscI 671 TGGCCA TGG^CCA 1340 TGGCCA NaeI 1013 GCCGGC GCC^GGC 1672 GCCGGC NarI 1775 GGCGCC GG^CGCC 2422 GGCGCC NcoI 1051 CCATGG C^CATGG 2547 CCATGG NgoMIV 1013 GCCGGC G^CCGGC 1672 GCCGGC NspI 2067 GCATGC [GA]CATG^[CT] 2405 GCATGC PmaCI 2615 CACGTG CAC^GTG 2922 CACGTG PmlI 2615 CACGTG CAC^GTG 2922 CACGTG PvuII 1278 CAGCTG CAG^CTG 1576 CAGCTG SfoI 1775 GGCGCC GGC^GCC 2422 GGCGCC SphI 2067 GCATGC GCATG^C 2405 GCATGC XcmI 1142 CCAGTGAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1343 CCACTGAGAGTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 115 enzymes) # ######################################### AatII AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XhoII XmaCI XmaI