(Circular) (Six-base) MAPSORT of: pFN21AE9259.seq Check: 2651 from: 1 to: 8335 pFN21AE9259 8335 bp With 182 enzymes: SgfI * April 20, 2012 13:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4271 4923 5513 4271 Size: 652 590 7093 Fragments arranged by size: 7093 652 590 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4338 4437 4883 6145 Size: 53 83 186 936 2802 99 446 1262 Cuts at: 6145 278 Size: 2468 Fragments arranged by size: 2802 2468 1262 936 446 186 99 83 53 AccI GT'mk_AC Cuts at: 1937 5678 1937 Size: 3741 4594 Acc65I G'GTAC_C Cuts at: 5656 5656 Size: 8335 AclI AA'CG_TT Cuts at: 1563 6462 6835 1563 Size: 4899 373 3063 Fragments arranged by size: 4899 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2797 2892 3648 5404 6398 7782 8050 1632 Size: 1165 95 756 1756 994 1384 268 1917 Fragments arranged by size: 1917 1756 1384 1165 994 756 268 95 AfeI AGC'GCT Cuts at: 4685 4685 Size: 8335 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8138 AflIII A'CryG_T Cuts at: 1203 4692 4928 7234 8211 1203 Size: 3489 236 2306 977 1327 Fragments arranged by size: 3489 2306 1327 977 236 AgeI A'CCGG_T Cuts at: 7166 7166 Size: 8335 AhdI GACnn_n'nnGTC Cuts at: 2773 2866 7064 2773 Size: 93 4198 4044 Fragments arranged by size: 4198 4044 93 AleI CACnn'nnGTG Cuts at: 1166 4931 5346 1166 Size: 3765 415 4155 Fragments arranged by size: 4155 3765 415 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4085 5132 1184 Size: 2901 1047 4387 Fragments arranged by size: 4387 2901 1047 AlwNI CAG_nnn'CTG Cuts at: 2210 2498 3692 6078 7650 2210 Size: 288 1194 2386 1572 2895 Fragments arranged by size: 2895 2386 1572 1194 288 ApaI G_GGCC'C Cuts at: 3828 3828 Size: 8335 ApaLI G'TGCA_C Cuts at: 6392 7548 6392 Size: 1156 7179 ApoI r'AATT_y Cuts at: 1493 5018 5644 5870 6022 8131 1493 Size: 3525 626 226 152 2109 1697 Fragments arranged by size: 3525 2109 1697 626 226 152 AseI AT'TA_AT Cuts at: 160 1032 6889 160 Size: 872 5857 1606 Fragments arranged by size: 5857 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8335 AvaI C'yCGr_G Cuts at: 1870 1943 2771 3487 3651 3722 4581 4679 4785 Size: 73 828 716 164 71 859 98 106 Cuts at: 4785 5427 5603 5660 1870 Size: 642 176 57 4545 Fragments arranged by size: 4545 859 828 716 642 176 164 106 98 73 71 57 BamHI G'GATC_C Cuts at: 2322 5665 6032 2322 Size: 3343 367 4625 Fragments arranged by size: 4625 3343 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3298 3515 3780 4427 5656 Size: 325 217 627 1511 217 265 647 1229 Cuts at: 5656 7111 618 Size: 1455 1842 Fragments arranged by size: 1842 1511 1455 1229 647 627 325 265 217 217 BanII G_rGCy'C Cuts at: 1391 2476 2525 2554 3137 3828 4094 4839 5610 Size: 1085 49 29 583 691 266 745 771 Cuts at: 5610 5654 1391 Size: 44 4072 Fragments arranged by size: 4072 1085 771 745 691 583 266 49 44 29 BbeI G_GCGC'C Cuts at: 3784 4431 3784 Size: 647 7688 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3589 5368 928 Size: 971 1690 1779 3895 Fragments arranged by size: 3895 1779 1690 971 BbvCI CC'TCA_GC Cuts at: 5229 5229 Size: 8335 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5531 5565 6526 6560 1903 Size: 34 3594 34 961 34 3678 Fragments arranged by size: 3678 3594 961 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6227 7437 6227 Size: 1210 7125 BclI T'GATC_A Cuts at: 1548 1548 Size: 8335 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3880 4965 Size: 107 122 71 631 605 337 1871 1085 Cuts at: 4965 5514 6946 136 Size: 549 1432 1525 Fragments arranged by size: 1871 1525 1432 1085 631 605 549 337 122 107 71 BglII A'GATC_T Cuts at: 2671 4101 8330 2671 Size: 1430 4229 2676 Fragments arranged by size: 4229 2676 1430 BlpI GC'TnA_GC Cuts at: 4316 4973 5752 4316 Size: 657 779 6899 Fragments arranged by size: 6899 779 657 Bme1580I G_kGCm'C Cuts at: 1790 3336 3518 3754 3828 4806 6396 7552 1790 Size: 1546 182 236 74 978 1590 1156 2573 Fragments arranged by size: 2573 1590 1546 1156 978 236 182 74 BmgBI CAC'GTC Cuts at: 1318 4693 4780 1318 Size: 3375 87 4873 Fragments arranged by size: 4873 3375 87 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2663 3141 3741 Size: 416 53 83 399 24 1246 478 600 Cuts at: 3741 3887 4483 5451 6066 7024 442 Size: 146 596 968 615 958 1753 Fragments arranged by size: 1753 1246 968 958 615 600 596 478 416 399 146 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8335 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5236 5629 5236 Size: 393 7942 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2187 2201 2493 2801 3200 3266 3545 Size: 863 85 14 292 308 399 66 279 Cuts at: 3545 4146 4217 4997 5095 5576 6995 1239 Size: 601 71 780 98 481 1419 2579 Fragments arranged by size: 2579 1419 863 780 601 481 399 308 292 279 98 85 71 66 14 Bpu10I CC'TnA_GC Cuts at: 3820 4635 5229 5592 3820 Size: 815 594 363 6563 Fragments arranged by size: 6563 815 594 363 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2312 3380 3618 6460 7325 7623 2312 Size: 1068 238 2842 865 298 3024 Fragments arranged by size: 3024 2842 1068 865 298 238 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3398 3443 6998 882 Size: 617 1899 45 3555 2219 Fragments arranged by size: 3555 2219 1899 617 45 BsaAI yAC'GTr Cuts at: 493 1204 4622 4929 6126 493 Size: 711 3418 307 1197 2702 Fragments arranged by size: 3418 2702 1197 711 307 BsaBI GATnn'nnATC Cuts at: 8329 8329 Size: 8335 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3781 4163 4335 4364 Size: 53 83 186 936 2248 382 172 29 Cuts at: 4364 4428 4434 4880 6142 6524 7159 275 Size: 64 6 446 1262 382 635 1451 Fragments arranged by size: 2248 1451 1262 936 635 446 382 382 186 172 83 64 53 29 6 BsaWI w'CCGG_w Cuts at: 4980 5436 6768 7166 7440 7587 7998 4980 Size: 456 1332 398 274 147 411 5317 Fragments arranged by size: 5317 1332 456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3673 3703 4463 4493 5441 Size: 30 165 30 2270 30 760 30 948 Cuts at: 5441 5471 1178 Size: 30 4042 Fragments arranged by size: 4042 2270 948 760 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2112 2732 2798 2921 2924 2927 2936 2960 Size: 926 620 66 123 3 3 9 24 Cuts at: 2960 2963 2993 3127 3132 3281 4262 4469 5462 Size: 3 30 134 5 149 981 207 993 Cuts at: 5462 1186 Size: 4059 Fragments arranged by size: 4059 993 981 926 620 207 149 134 123 66 30 24 9 5 3 3 3 BseYI C'CCAG_C Cuts at: 2329 2363 3188 3218 5151 7538 8034 2329 Size: 34 825 30 1933 2387 496 2630 Fragments arranged by size: 2630 2387 1933 825 496 34 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3301 3490 3678 4238 4454 4553 4909 Size: 573 1565 189 188 560 216 99 356 Cuts at: 4909 1163 Size: 4589 Fragments arranged by size: 4589 1565 573 560 356 216 189 188 99 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2040 3678 4900 5473 5785 6546 Size: 275 186 39 1638 1222 573 312 761 Cuts at: 6546 7574 1540 Size: 1028 2301 Fragments arranged by size: 2301 1638 1222 1028 761 573 312 275 186 39 BsiHKAI G_wGCw'C Cuts at: 3444 4322 4389 5069 5610 6396 6481 7552 3444 Size: 878 67 680 541 786 85 1071 4227 Fragments arranged by size: 4227 1071 878 786 680 541 85 67 BsmI GAATG_Cn' Cuts at: 4525 5846 5939 4525 Size: 1321 93 6921 Fragments arranged by size: 6921 1321 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2786 3075 4871 2786 Size: 289 1796 6250 Fragments arranged by size: 6250 1796 289 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2476 2525 2554 3137 3336 3444 3518 Size: 399 686 49 29 583 199 108 74 Cuts at: 3518 3754 3828 4094 4322 4389 4806 4839 5069 Size: 236 74 266 228 67 417 33 230 Cuts at: 5069 5610 5654 6396 6481 7552 1391 Size: 541 44 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 686 583 541 417 399 266 236 230 228 199 108 85 74 74 67 49 44 33 29 BspEI T'CCGG_A Cuts at: 5436 7998 5436 Size: 2562 5773 BspHI T'CATG_A Cuts at: 6224 7954 6224 Size: 1730 6605 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4271 4923 5403 5513 5690 844 Size: 3427 652 480 110 177 3489 Fragments arranged by size: 3489 3427 652 480 177 110 BsrBI CCG'CTC Cuts at: 1394 1823 6222 1394 Size: 429 4399 3507 Fragments arranged by size: 4399 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3388 5547 6830 7004 65 Size: 1183 2140 2159 1283 174 1396 Fragments arranged by size: 2159 2140 1396 1283 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3018 3673 3677 6979 7166 1700 Size: 110 1208 655 4 3302 187 2869 Fragments arranged by size: 3302 2869 1208 655 187 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8335 BssHII G'CGCG_C Cuts at: 1925 4523 1925 Size: 2598 5737 BssSI C'ACGA_G Cuts at: 2518 3114 6395 7407 2518 Size: 596 3281 1012 3446 Fragments arranged by size: 3446 3281 1012 596 BstAPI GCAn_nnn'nTGC Cuts at: 794 3692 4983 794 Size: 2898 1291 4146 Fragments arranged by size: 4146 2898 1291 BstBI TT'CG_AA Cuts at: 7139 7139 Size: 8335 BstEII G'GTnAC_C Cuts at: 1184 2662 3640 3664 1184 Size: 1478 978 24 5855 Fragments arranged by size: 5855 1478 978 24 BstXI CCAn_nnnn'nTGG Cuts at: 3427 4352 5554 3427 Size: 925 1202 6208 Fragments arranged by size: 6208 1202 925 BstYI r'GATC_y Cuts at: 1972 2322 2671 4101 5665 6032 6417 6434 7886 Size: 350 349 1430 1564 367 385 17 1452 Cuts at: 7886 7972 7984 8330 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1564 1452 1430 385 367 350 349 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3139 4845 3139 Size: 1706 6629 BtgI C'CryG_G Cuts at: 513 1065 2004 2311 3056 3098 3864 4095 4179 Size: 552 939 307 745 42 766 231 84 Cuts at: 4179 4552 513 Size: 373 4296 Fragments arranged by size: 4296 939 766 745 552 373 307 231 84 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4486 4558 5395 487 Size: 683 56 3260 72 837 3427 Fragments arranged by size: 3427 3260 837 683 72 56 BtsI GCAGTG_nn' Cuts at: 3478 3681 4149 4921 5851 6645 6665 3478 Size: 203 468 772 930 794 20 5148 Fragments arranged by size: 5148 930 794 772 468 203 20 ClaI AT'CG_AT Cuts at: 1554 4896 1554 Size: 3342 4993 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8300 DraI TTT'AAA Cuts at: 5639 5639 Size: 8335 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7342 809 Size: 507 6026 1802 Fragments arranged by size: 6026 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2676 3016 Size: 54 1425 50 275 18 207 639 340 Cuts at: 3016 3345 3675 4966 5782 6671 8300 8 Size: 329 330 1291 816 889 1629 43 Fragments arranged by size: 1629 1425 1291 889 816 639 340 330 329 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2037 3675 5782 1537 Size: 275 225 1638 2107 4090 Fragments arranged by size: 4090 2107 1638 275 225 EarI CTCTTCn'nnn_ Cuts at: 1353 2362 2898 2913 2931 2937 2949 2961 5267 Size: 1009 536 15 18 6 12 12 2306 Cuts at: 5267 6265 1353 Size: 998 3423 Fragments arranged by size: 3423 2306 1009 998 536 18 15 12 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3949 6922 7296 7442 387 Size: 1064 138 2360 2973 374 146 1280 Fragments arranged by size: 2973 2360 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5608 5608 Size: 8335 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2187 2201 2493 2797 2801 2892 Size: 393 470 85 14 292 304 4 91 Cuts at: 2892 3200 3266 3545 3648 4146 4217 4997 5095 Size: 308 66 279 103 498 71 780 98 Cuts at: 5095 5404 5576 6398 6995 7782 8050 1239 Size: 309 172 822 597 787 268 1524 Fragments arranged by size: 1524 822 787 780 597 498 470 393 309 308 304 292 279 268 172 103 98 91 85 71 66 14 4 EcoNI CCTnn'n_nnAGG Cuts at: 2159 2714 3710 3902 2159 Size: 555 996 192 6592 Fragments arranged by size: 6592 996 555 192 EcoO109I rG'GnC_Cy Cuts at: 2053 2305 2767 2775 3143 3163 3174 3198 3824 Size: 252 462 8 368 20 11 24 626 Cuts at: 3824 3825 3895 3907 4669 5187 2053 Size: 1 70 12 762 518 5201 Fragments arranged by size: 5201 762 626 518 462 368 252 70 24 20 12 11 8 1 EcoRI G'AATT_C Cuts at: 5644 6022 5644 Size: 378 7957 FspI TGC'GCA Cuts at: 6841 6841 Size: 8335 HaeII r_GCGC'y Cuts at: 1678 2075 3784 4431 4497 4687 7482 1678 Size: 397 1709 647 66 190 2795 2531 Fragments arranged by size: 2795 2531 1709 647 397 190 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2633 2656 2665 2688 2759 Size: 32 135 32 1061 23 9 23 71 Cuts at: 2759 2791 2819 2851 2852 2884 2966 2998 4928 Size: 32 28 32 1 32 82 32 1930 Cuts at: 4928 4960 5020 5052 6976 7008 7050 7082 1373 Size: 32 60 32 1924 32 42 32 2626 Fragments arranged by size: 2626 1930 1924 1061 135 82 71 60 42 32 32 32 32 32 32 32 32 32 32 28 23 23 9 1 HincII GTy'rAC Cuts at: 669 1657 1938 4339 4546 4819 5629 5679 5925 Size: 988 281 2401 207 273 810 50 246 Cuts at: 5925 669 Size: 3079 Fragments arranged by size: 3079 2401 988 810 281 273 246 207 50 HindIII A'AGCT_T Cuts at: 748 748 Size: 8335 HpaI GTT'AAC Cuts at: 5925 5925 Size: 8335 KasI G'GCGC_C Cuts at: 3780 4427 3780 Size: 647 7688 KpnI G_GTAC'C Cuts at: 5660 5660 Size: 8335 MfeI C'AATT_G Cuts at: 5934 5934 Size: 8335 MluI A'CGCG_T Cuts at: 8211 8211 Size: 8335 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2694 2883 3996 4713 4750 4977 7449 7633 Size: 1389 189 1113 717 37 227 2472 184 Cuts at: 7633 1305 Size: 2007 Fragments arranged by size: 2472 2007 1389 1113 717 227 189 184 37 MscI TGG'CCA Cuts at: 10 64 1489 1832 2678 3347 10 Size: 54 1425 343 846 669 4998 Fragments arranged by size: 4998 1425 846 669 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4400 4931 5114 5346 6293 6652 6811 Size: 648 3234 531 183 232 947 359 159 Cuts at: 6811 518 Size: 2042 Fragments arranged by size: 3234 2042 947 648 531 359 232 183 159 MspA1I CmG'CkG Cuts at: 1606 2030 2060 3100 3285 3583 4159 5041 5751 Size: 424 30 1040 185 298 576 882 710 Cuts at: 5751 6428 7576 7821 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1040 882 710 677 576 424 298 245 185 30 NaeI GCC'GGC Cuts at: 3020 3679 3020 Size: 659 7676 NarI GG'CG_CC Cuts at: 3781 4428 3781 Size: 647 7688 NcoI C'CATG_G Cuts at: 513 1065 2004 3056 4552 513 Size: 552 939 1052 1496 4296 Fragments arranged by size: 4296 1496 1052 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8335 NgoMIV G'CCGG_C Cuts at: 3018 3677 3018 Size: 659 7676 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8335 NotI GC'GGCC_GC Cuts at: 2037 5782 2037 Size: 3745 4590 NspI r_CATG'y Cuts at: 1132 1229 4076 4414 5693 7238 1132 Size: 97 2847 338 1279 1545 2229 Fragments arranged by size: 2847 2229 1545 1279 338 97 PasI CC'CwG_GG Cuts at: 1348 2476 1348 Size: 1128 7207 PciI A'CATG_T Cuts at: 7234 7234 Size: 8335 PflMI CCAn_nnn'nTGG Cuts at: 1108 2270 2443 2579 2653 4374 1108 Size: 1162 173 136 74 1721 5069 Fragments arranged by size: 5069 1721 1162 173 136 74 PfoI T'CCnGG_A Cuts at: 3522 3918 5013 3522 Size: 396 1095 6844 Fragments arranged by size: 6844 1095 396 PmeI GTTT'AAAC Cuts at: 5639 5639 Size: 8335 PmlI CAC'GTG Cuts at: 4622 4929 4622 Size: 307 8028 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6404 6436 7917 7949 6404 Size: 32 1481 32 6790 Fragments arranged by size: 6790 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2305 2767 2775 3163 3174 3198 4669 5187 2305 Size: 462 8 388 11 24 1471 518 5453 Fragments arranged by size: 5453 1471 518 462 388 24 11 8 PshAI GACnn'nnGTC Cuts at: 1613 2814 1613 Size: 1201 7134 PsiI TTA'TAA Cuts at: 5905 5905 Size: 8335 PspOMI G'GGCC_C Cuts at: 3824 3824 Size: 8335 PstI C_TGCA'G Cuts at: 830 2029 2488 3688 3996 4122 4384 4920 5172 Size: 1199 459 1200 308 126 262 536 252 Cuts at: 5172 5687 830 Size: 515 3478 Fragments arranged by size: 3478 1200 1199 536 515 459 308 262 252 126 PvuI CG_AT'CG Cuts at: 2001 4900 2001 Size: 2899 5436 PvuII CAG'CTG Cuts at: 3285 3583 3285 Size: 298 8037 SacI G_AGCT'C Cuts at: 5610 5610 Size: 8335 SacII CC_GC'GG Cuts at: 3101 3101 Size: 8335 SalI G'TCGA_C Cuts at: 1936 5677 1936 Size: 3741 4594 SanDI GG'GwC_CC Cuts at: 2767 2775 3174 3198 2767 Size: 8 399 24 7904 Fragments arranged by size: 7904 399 24 8 SbfI CC_TGCA'GG Cuts at: 5687 5687 Size: 8335 ScaI AGT'ACT Cuts at: 6583 8295 6583 Size: 1712 6623 SfcI C'TryA_G Cuts at: 826 1045 2025 2250 2484 3684 3992 4118 4237 Size: 219 980 225 234 1200 308 126 119 Cuts at: 4237 4380 4916 5123 5168 5683 6818 7499 7690 Size: 143 536 207 45 515 1135 681 191 Cuts at: 7690 826 Size: 1471 Fragments arranged by size: 1471 1200 1135 980 681 536 515 308 234 225 219 207 191 143 126 119 45 SfoI GGC'GCC Cuts at: 3782 4429 3782 Size: 647 7688 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8335 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8335 SmaI CCC'GGG Cuts at: 1872 2773 3489 3653 3724 5662 1872 Size: 901 716 164 71 1938 4545 Fragments arranged by size: 4545 1938 901 716 164 71 SmlI C'TyrA_G Cuts at: 820 1017 1943 2291 3395 3633 6439 7340 7602 Size: 197 926 348 1104 238 2806 901 262 Cuts at: 7602 820 Size: 1553 Fragments arranged by size: 2806 1553 1104 926 901 348 262 238 197 SnaBI TAC'GTA Cuts at: 493 6126 493 Size: 5633 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8335 SphI G_CATG'C Cuts at: 1132 4076 4414 5693 1132 Size: 2944 338 1279 3774 Fragments arranged by size: 3774 2944 1279 338 SrfI GCCC'GGGC Cuts at: 3489 3489 Size: 8335 SspI AAT'ATT Cuts at: 5 52 5498 6259 5 Size: 47 5446 761 2081 Fragments arranged by size: 5446 2081 761 47 StuI AGG'CCT Cuts at: 1517 3569 1517 Size: 2052 6283 StyI C'CwwG_G Cuts at: 513 1065 2004 2160 2538 3056 3105 4552 5774 Size: 552 939 156 378 518 49 1447 1222 Cuts at: 5774 513 Size: 3074 Fragments arranged by size: 3074 1447 1222 939 552 518 378 156 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2792 3049 3316 4925 6362 6379 6532 Size: 1255 1212 257 267 1609 1437 17 153 Cuts at: 6532 6717 7162 325 Size: 185 445 1498 Fragments arranged by size: 1609 1498 1437 1255 1212 445 267 257 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3153 4857 5292 Size: 274 80 33 51 1443 1176 1704 435 Cuts at: 5292 6581 8293 96 Size: 1289 1712 138 Fragments arranged by size: 1712 1704 1443 1289 1176 435 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3673 3705 4463 4495 8295 8327 3673 Size: 32 758 32 3800 32 3681 Fragments arranged by size: 3800 3681 758 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8335 XbaI T'CTAG_A Cuts at: 5671 5671 Size: 8335 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3154 3355 3154 Size: 201 8134 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8335 XmaI C'CCGG_G Cuts at: 1870 2771 3487 3651 3722 5660 1870 Size: 901 716 164 71 1938 4545 Fragments arranged by size: 4545 1938 901 716 164 71 XmnI GAAnn'nnTTC Cuts at: 4512 6464 4512 Size: 1952 6383 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4336 4435 4881 6143 Size: 53 83 186 936 2802 99 446 1262 Cuts at: 6143 276 Size: 2468 Fragments arranged by size: 2802 2468 1262 936 446 186 99 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SapI SexAI SfiI SwaI