Restriction map for
pFN21AA0325
(Linear) (Six-base) MAPSORT of: KIAA0325.seq Check: 9024 from: 1 to: 13938
KIAA0325.orf
With 182 enzymes: SgfI *
May 20, 2014 13:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 107 3693 3948 4685 5910 11943 12263 13938
Size: 107 3586 255 737 1225 6033 320 1675
Fragments arranged by size:
6033 3586 1675 1225 737 320 255 107
AatII G_ACGT'C
Cuts at: 0 263 13938
Size: 263 13675
AccI GT'mk_AC
Cuts at: 0 3473 4634 8651 9635 12863 13826 13938
Size: 3473 1161 4017 984 3228 963 112
Fragments arranged by size:
4017 3473 3228 1161 984 963 112
Acc65I G'GTAC_C
Cuts at: 0 3008 13938
Size: 3008 10930
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1273 1357 2065 2810 3148 3155 3814
Size: 524 749 84 708 745 338 7 659
Cuts at: 3814 3889 6206 6456 6565 6733 7800 7915 8584
Size: 75 2317 250 109 168 1067 115 669
Cuts at: 8584 8710 10456 10839 11338 11557 11695 12307 13051
Size: 126 1746 383 499 219 138 612 744
Cuts at: 13051 13255 13428 13938
Size: 204 173 510
Fragments arranged by size:
2317 1746 1067 749 745 744 708 669
659 612 524 510 499 383 338 250
219 204 173 168 138 126 115 109
84 75 7
AfeI AGC'GCT
Cuts at: 0 241 925 2503 3583 7462 8426 13938
Size: 241 684 1578 1080 3879 964 5512
Fragments arranged by size:
5512 3879 1578 1080 964 684 241
AflII C'TTAA_G
Cuts at: 0 2197 13938
Size: 2197 11741
AflIII A'CryG_T
Cuts at: 0 77 6488 6843 10112 10211 12017 12159 12659
Size: 77 6411 355 3269 99 1806 142 500
Cuts at: 12659 13523 13938
Size: 864 415
Fragments arranged by size:
6411 3269 1806 864 500 415 355 142
99 77
AhdI GACnn_n'nnGTC
Cuts at: 0 1848 12636 13104 13938
Size: 1848 10788 468 834
Fragments arranged by size:
10788 1848 834 468
AleI CACnn'nnGTG
Cuts at: 0 1790 1913 3514 6515 6844 7567 12100 12597
Size: 1790 123 1601 3001 329 723 4533 497
Cuts at: 12597 13938
Size: 1341
Fragments arranged by size:
4533 3001 1790 1601 1341 723 497 329
123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 127 10261 12371 13938
Size: 127 10134 2110 1567
Fragments arranged by size:
10134 2110 1567 127
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2386 2418 12799 12831 13210 13242 13938
Size: 2386 32 10381 32 379 32 696
Fragments arranged by size:
10381 2386 696 379 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 808 2043 2442 2515 3925 5383 5743 7693
Size: 808 1235 399 73 1410 1458 360 1950
Cuts at: 7693 8152 8624 9909 10254 11565 11778 12789 13078
Size: 459 472 1285 345 1311 213 1011 289
Cuts at: 13078 13182 13938
Size: 104 756
Fragments arranged by size:
1950 1458 1410 1311 1285 1235 1011 808
756 472 459 399 360 345 289 213
104 73
ApaI G_GGCC'C
Cuts at: 0 6674 8221 13938
Size: 6674 1547 5717
Fragments arranged by size:
6674 5717 1547
ApaLI G'TGCA_C
Cuts at: 0 10874 13929 13938
Size: 10874 3055 9
Fragments arranged by size:
10874 3055 9
ApoI r'AATT_y
Cuts at: 0 289 602 764 1222 1402 3049 3388 3421
Size: 289 313 162 458 180 1647 339 33
Cuts at: 3421 4083 4381 5593 9322 10792 11209 11710 12616
Size: 662 298 1212 3729 1470 417 501 906
Cuts at: 12616 13938
Size: 1322
Fragments arranged by size:
3729 1647 1470 1322 1212 906 662 501
458 417 339 313 298 289 180 162
33
AvaI C'yCGr_G
Cuts at: 0 10 2139 2168 5033 6675 6730 8214 10990
Size: 10 2129 29 2865 1642 55 1484 2776
Cuts at: 10990 11042 11081 11615 13938
Size: 52 39 534 2323
Fragments arranged by size:
2865 2776 2323 2129 1642 1484 534 55
52 39 29 10
BamHI G'GATC_C
Cuts at: 0 755 2993 6921 11046 11768 13218 13890 13938
Size: 755 2238 3928 4125 722 1450 672 48
Fragments arranged by size:
4125 3928 2238 1450 755 722 672 48
BanI G'GyrC_C
Cuts at: 0 111 141 3008 5854 6435 7653 8245 9048
Size: 111 30 2867 2846 581 1218 592 803
Cuts at: 9048 11680 12399 13115 13404 13498 13648 13938
Size: 2632 719 716 289 94 150 290
Fragments arranged by size:
2867 2846 2632 1218 803 719 716 592
581 290 289 150 111 94 30
BanII G_rGCy'C
Cuts at: 0 11 883 3566 6028 6674 6836 7369 8221
Size: 11 872 2683 2462 646 162 533 852
Cuts at: 8221 9149 9614 10232 11696 13616 13938
Size: 928 465 618 1464 1920 322
Fragments arranged by size:
2683 2462 1920 1464 928 872 852 646
618 533 465 322 162 11
BbeI G_GCGC'C
Cuts at: 0 145 13119 13502 13652 13938
Size: 145 12974 383 150 286
Fragments arranged by size:
12974 383 286 150 145
BbsI GAAGACnn'nnnn_
Cuts at: 0 386 431 716 2511 3793 6259 6897 6994
Size: 386 45 285 1795 1282 2466 638 97
Cuts at: 6994 7903 8915 10669 13093 13938
Size: 909 1012 1754 2424 845
Fragments arranged by size:
2466 2424 1795 1754 1282 1012 909 845
638 386 285 97 45
BbvCI CC'TCA_GC
Cuts at: 0 1523 8266 8758 12472 13938
Size: 1523 6743 492 3714 1466
Fragments arranged by size:
6743 3714 1523 1466 492
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4920 4954 8539 8573 12154 12188 13938
Size: 4920 34 3585 34 3581 34 1750
Fragments arranged by size:
4920 3585 3581 1750 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 8352 10766 13938
Size: 8352 2414 3172
Fragments arranged by size:
8352 3172 2414
BclI T'GATC_A
Cuts at: 0 635 2552 5474 7905 10763 10982 13016 13352
Size: 635 1917 2922 2431 2858 219 2034 336
Cuts at: 13352 13938
Size: 586
Fragments arranged by size:
2922 2858 2431 2034 1917 635 586 336
219
BfrBI ATG'CAT
Cuts at: 0 1206 13938
Size: 1206 12732
BglI GCCn_nnn'nGGC
Cuts at: 0 1639 3904 5689 6135 7828 10384 12957 13535
Size: 1639 2265 1785 446 1693 2556 2573 578
Cuts at: 13535 13938
Size: 403
Fragments arranged by size:
2573 2556 2265 1785 1693 1639 578 446
403
BglII A'GATC_T
Cuts at: 0 2090 3222 3434 8237 8922 9840 11070 13938
Size: 2090 1132 212 4803 685 918 1230 2868
Fragments arranged by size:
4803 2868 2090 1230 1132 918 685 212
BlpI GC'TnA_GC
Cuts at: 0 2505 5344 5515 7105 11812 13183 13938
Size: 2505 2839 171 1590 4707 1371 755
Fragments arranged by size:
4707 2839 2505 1590 1371 755 171
Bme1580I G_kGCm'C
Cuts at: 0 1131 1192 1627 6674 8221 8478 9111 10878
Size: 1131 61 435 5047 1547 257 633 1767
Cuts at: 10878 11683 11912 12097 12531 13933 13938
Size: 805 229 185 434 1402 5
Fragments arranged by size:
5047 1767 1547 1402 1131 805 633 435
434 257 229 185 61 5
BmgBI CAC'GTC
Cuts at: 0 78 6489 7283 9189 13106 13938
Size: 78 6411 794 1906 3917 832
Fragments arranged by size:
6411 3917 1906 832 794 78
BmrI ACTGGGnnnn_n'
Cuts at: 0 2112 3789 3935 5738 8641 12604 12738 13938
Size: 2112 1677 146 1803 2903 3963 134 1200
Fragments arranged by size:
3963 2903 2112 1803 1677 1200 146 134
BmtI G_CTAG'C
Cuts at: 0 9484 13938
Size: 9484 4454
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 51 3241 3483 5025 6255 7012 10445 13938
Size: 51 3190 242 1542 1230 757 3433 3493
Fragments arranged by size:
3493 3433 3190 1542 1230 757 242 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 175 187 205 761 1740 4997 5908
Size: 145 30 12 18 556 979 3257 911
Cuts at: 5908 6518 6607 7348 7477 7529 7851 7865 8128
Size: 610 89 741 129 52 322 14 263
Cuts at: 8128 8458 9034 9364 9601 9706 9982 11329 11470
Size: 330 576 330 237 105 276 1347 141
Cuts at: 11470 11940 12346 12826 13627 13938
Size: 470 406 480 801 311
Fragments arranged by size:
3257 1347 979 911 801 741 610 576
556 480 470 406 330 330 322 311
276 263 237 145 141 129 105 89
52 30 18 14 12
Bpu10I CC'TnA_GC
Cuts at: 0 1523 5543 8266 8758 9613 12472 13938
Size: 1523 4020 2723 492 855 2859 1466
Fragments arranged by size:
4020 2859 2723 1523 1466 855 492
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1182 8771 9208 9684 11437 11745 13177 13938
Size: 1182 7589 437 476 1753 308 1432 761
Fragments arranged by size:
7589 1753 1432 1182 761 476 437 308
BsaI GGTCTCn'nnnn_
Cuts at: 0 1675 4802 5281 7640 7861 8424 8434 9061
Size: 1675 3127 479 2359 221 563 10 627
Cuts at: 9061 10307 11377 13938
Size: 1246 1070 2561
Fragments arranged by size:
3127 2561 2359 1675 1246 1070 627 563
479 221 10
BsaAI yAC'GTr
Cuts at: 0 2028 6846 8190 8754 12662 13524 13706 13938
Size: 2028 4818 1344 564 3908 862 182 232
Fragments arranged by size:
4818 3908 2028 1344 862 564 232 182
BsaBI GATnn'nnATC
Cuts at: 0 791 6920 8921 9508 13938
Size: 791 6129 2001 587 4430
Fragments arranged by size:
6129 4430 2001 791 587
BsaHI Gr'CG_yC
Cuts at: 0 142 260 1410 2735 10082 10265 10850 12949
Size: 142 118 1150 1325 7347 183 585 2099
Cuts at: 12949 13116 13499 13649 13938
Size: 167 383 150 289
Fragments arranged by size:
7347 2099 1325 1150 585 383 289 183
167 150 142 118
BsaWI w'CCGG_w
Cuts at: 0 606 2249 6107 10418 12702 13938
Size: 606 1643 3858 4311 2284 1236
Fragments arranged by size:
4311 3858 2284 1643 1236 606
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2808 2838 2969 2999 5033 5038 5063 5068
Size: 2808 30 131 30 2034 5 25 5
Cuts at: 5068 6578 6608 9907 9937 11374 11404 11464 11494
Size: 1510 30 3299 30 1437 30 60 30
Cuts at: 11494 11875 11905 12319 12349 12799 12829 13938
Size: 381 30 414 30 450 30 1109
Fragments arranged by size:
3299 2808 2034 1510 1437 1109 450 414
381 131 60 30 30 30 30 30
30 30 30 30 25 5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 2401 5059 5905 6553 7231 8536 9157
Size: 184 2217 2658 846 648 678 1305 621
Cuts at: 9157 9598 10084 11368 12922 13624 13938
Size: 441 486 1284 1554 702 314
Fragments arranged by size:
2658 2217 1554 1305 1284 846 702 678
648 621 486 441 314 184
BseYI C'CCAG_C
Cuts at: 0 1817 2015 4466 4565 4694 5240 5697 5915
Size: 1817 198 2451 99 129 546 457 218
Cuts at: 5915 7539 9981 10510 11393 12960 13772 13938
Size: 1624 2442 529 883 1567 812 166
Fragments arranged by size:
2451 2442 1817 1624 1567 883 812 546
529 457 218 198 166 129 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 2137 2455 2653 2952 3538 3976 4639
Size: 82 2055 318 198 299 586 438 663
Cuts at: 4639 4682 5944 6317 7369 12707 12937 13123 13147
Size: 43 1262 373 1052 5338 230 186 24
Cuts at: 13147 13865 13938
Size: 718 73
Fragments arranged by size:
5338 2055 1262 1052 718 663 586 438
373 318 299 230 198 186 82 73
43 24
BsiEI CG_ry'CG
Cuts at: 0 41 59 149 6135 7568 11997 13938
Size: 41 18 90 5986 1433 4429 1941
Fragments arranged by size:
5986 4429 1941 1433 90 41 18
BsiHKAI G_wGCw'C
Cuts at: 0 3566 7111 7316 7334 8066 9149 10426 10878
Size: 3566 3545 205 18 732 1083 1277 452
Cuts at: 10878 12133 13616 13933 13938
Size: 1255 1483 317 5
Fragments arranged by size:
3566 3545 1483 1277 1255 1083 732 452
317 205 18 5
BsmI GAATG_Cn'
Cuts at: 0 349 447 1734 3066 9477 9702 9885 12339
Size: 349 98 1287 1332 6411 225 183 2454
Cuts at: 12339 12488 13938
Size: 149 1450
Fragments arranged by size:
6411 2454 1450 1332 1287 349 225 183
149 98
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4515 6421 7666 8497 8934 9062 11350 12501
Size: 4515 1906 1245 831 437 128 2288 1151
Cuts at: 12501 13938
Size: 1437
Fragments arranged by size:
4515 2288 1906 1437 1245 1151 831 437
128
Bsp1286I G_dGCh'C
Cuts at: 0 11 883 1131 1192 1627 3566 6028 6674
Size: 11 872 248 61 435 1939 2462 646
Cuts at: 6674 6836 7111 7316 7334 7369 8066 8221 8478
Size: 162 275 205 18 35 697 155 257
Cuts at: 8478 9111 9149 9614 10232 10426 10878 11683 11696
Size: 633 38 465 618 194 452 805 13
Cuts at: 11696 11912 12097 12133 12531 13616 13933 13938
Size: 216 185 36 398 1085 317 5
Fragments arranged by size:
2462 1939 1085 872 805 697 646 633
618 465 452 435 398 317 275 257
248 216 205 194 185 162 155 61
38 36 35 18 13 11 5
BspEI T'CCGG_A
Cuts at: 0 606 2249 6107 13938
Size: 606 1643 3858 7831
Fragments arranged by size:
7831 3858 1643 606
BspHI T'CATG_A
Cuts at: 0 2877 4247 13938
Size: 2877 1370 9691
Fragments arranged by size:
9691 2877 1370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 207 2380 2760 3693 3722 3776 3948
Size: 107 100 2173 380 933 29 54 172
Cuts at: 3948 4685 4826 5727 5910 9867 11943 12263 12641
Size: 737 141 901 183 3957 2076 320 378
Cuts at: 12641 13938
Size: 1297
Fragments arranged by size:
3957 2173 2076 1297 933 901 737 380
378 320 183 172 141 107 100 54
29
BsrBI CCG'CTC
Cuts at: 0 1696 2391 4866 5895 8304 10016 11409 12072
Size: 1696 695 2475 1029 2409 1712 1393 663
Cuts at: 12072 13911 13938
Size: 1839 27
Fragments arranged by size:
2475 2409 1839 1712 1696 1393 1029 695
663 27
BsrDI GCAATG_nn'
Cuts at: 0 468 2456 3579 5355 5825 6309 8603 10245
Size: 468 1988 1123 1776 470 484 2294 1642
Cuts at: 10245 10505 10538 13938
Size: 260 33 3400
Fragments arranged by size:
3400 2294 1988 1776 1642 1123 484 470
468 260 33
BsrFI r'CCGG_y
Cuts at: 0 144 6071 6134 7517 13411 13938
Size: 144 5927 63 1383 5894 527
Fragments arranged by size:
5927 5894 1383 527 144 63
BsrGI T'GTAC_A
Cuts at: 0 2496 3021 7742 8615 13938
Size: 2496 525 4721 873 5323
Fragments arranged by size:
5323 4721 2496 873 525
BssSI C'ACGA_G
Cuts at: 0 9139 9562 13938
Size: 9139 423 4376
Fragments arranged by size:
9139 4376 423
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4680 5340 9594 11907 12345 13938
Size: 4680 660 4254 2313 438 1593
Fragments arranged by size:
4680 4254 2313 1593 660 438
BstBI TT'CG_AA
Cuts at: 0 13871 13938
Size: 13871 67
BstEII G'GTnAC_C
Cuts at: 0 5358 7296 10888 11337 13812 13938
Size: 5358 1938 3592 449 2475 126
Fragments arranged by size:
5358 3592 2475 1938 449 126
BstXI CCAn_nnnn'nTGG
Cuts at: 0 345 1047 3620 5767 7293 8125 10296 10842
Size: 345 702 2573 2147 1526 832 2171 546
Cuts at: 10842 11658 12599 13938
Size: 816 941 1339
Fragments arranged by size:
2573 2171 2147 1526 1339 941 832 816
702 546 345
BstYI r'GATC_y
Cuts at: 0 755 792 1097 2090 2993 3222 3434 4829
Size: 755 37 305 993 903 229 212 1395
Cuts at: 4829 5828 6921 7241 8237 8922 9339 9840 10010
Size: 999 1093 320 996 685 417 501 170
Cuts at: 10010 11046 11070 11768 12710 13218 13373 13890 13938
Size: 1036 24 698 942 508 155 517 48
Fragments arranged by size:
1395 1093 1036 999 996 993 942 903
755 698 685 517 508 501 417 320
305 229 212 170 155 48 37 24
Bsu36I CC'TnA_GG
Cuts at: 0 1450 13550 13938
Size: 1450 12100 388
Fragments arranged by size:
12100 1450 388
BtgI C'CryG_G
Cuts at: 0 1040 1791 3746 4461 7639 8315 9926 11441
Size: 1040 751 1955 715 3178 676 1611 1515
Cuts at: 11441 11684 12008 12380 12715 13202 13862 13938
Size: 243 324 372 335 487 660 76
Fragments arranged by size:
3178 1955 1611 1515 1040 751 715 676
660 487 372 335 324 243 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 6242 6929 10314 13938
Size: 6242 687 3385 3624
Fragments arranged by size:
6242 3624 3385 687
BtsI GCAGTG_nn'
Cuts at: 0 473 1129 2657 3503 3948 4665 5932 5941
Size: 473 656 1528 846 445 717 1267 9
Cuts at: 5941 6179 7580 8306 9100 10424 12925 13135 13562
Size: 238 1401 726 794 1324 2501 210 427
Cuts at: 13562 13938
Size: 376
Fragments arranged by size:
2501 1528 1401 1324 1267 846 794 726
717 656 473 445 427 376 238 210
9
ClaI AT'CG_AT
Cuts at: 0 4028 7604 13938
Size: 4028 3576 6334
Fragments arranged by size:
6334 4028 3576
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2829 2864 6428 6463 13938
Size: 2829 35 3564 35 7475
Fragments arranged by size:
7475 3564 2829 35 35
DraI TTT'AAA
Cuts at: 0 5058 6219 13938
Size: 5058 1161 7719
Fragments arranged by size:
7719 5058 1161
DraIII CAC_nnn'GTG
Cuts at: 0 228 421 5340 5947 7683 8389 9192 9912
Size: 228 193 4919 607 1736 706 803 720
Cuts at: 9912 12090 13006 13938
Size: 2178 916 932
Fragments arranged by size:
4919 2178 1736 932 916 803 720 706
607 228 193
DrdI GACnn_nn'nnGTC
Cuts at: 0 217 2578 5635 13938
Size: 217 2361 3057 8303
Fragments arranged by size:
8303 3057 2361 217
EaeI y'GGCC_r
Cuts at: 0 38 56 146 3479 3576 4293 4514 5138
Size: 38 18 90 3333 97 717 221 624
Cuts at: 5138 5757 6035 6116 7058 7136 7754 9035 9506
Size: 619 278 81 942 78 618 1281 471
Cuts at: 9506 9929 11949 12005 12677 13938
Size: 423 2020 56 672 1261
Fragments arranged by size:
3333 2020 1281 1261 942 717 672 624
619 618 471 423 278 221 97 90
81 78 56 38 18
EagI C'GGCC_G
Cuts at: 0 38 56 146 13938
Size: 38 18 90 13792
Fragments arranged by size:
13792 90 38 18
EarI CTCTTCn'nnn_
Cuts at: 0 169 1645 1859 2921 3038 3095 3815 3941
Size: 169 1476 214 1062 117 57 720 126
Cuts at: 3941 4483 4544 6246 6274 6304 6475 6682 6841
Size: 542 61 1702 28 30 171 207 159
Cuts at: 6841 7378 7819 8500 8654 9464 10432 10436 11314
Size: 537 441 681 154 810 968 4 878
Cuts at: 11314 11593 12376 13938
Size: 279 783 1562
Fragments arranged by size:
1702 1562 1476 1062 968 878 810 783
720 681 542 537 441 279 214 207
171 169 159 154 126 117 61 57
30 28 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 5324 5430 7714 11809 13938
Size: 88 5236 106 2284 4095 2129
Fragments arranged by size:
5236 4095 2284 2129 106 88
EcoICRI GAG'CTC
Cuts at: 0 3564 9147 13614 13938
Size: 3564 5583 4467 324
Fragments arranged by size:
5583 4467 3564 324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 175 187 205 524 761 1273 1357
Size: 145 30 12 18 319 237 512 84
Cuts at: 1357 1740 2065 2810 3148 3155 3814 3889 4997
Size: 383 325 745 338 7 659 75 1108
Cuts at: 4997 5908 6206 6456 6518 6565 6607 6733 7348
Size: 911 298 250 62 47 42 126 615
Cuts at: 7348 7477 7529 7800 7851 7865 7915 8128 8458
Size: 129 52 271 51 14 50 213 330
Cuts at: 8458 8584 8710 9034 9364 9601 9706 9982 10456
Size: 126 126 324 330 237 105 276 474
Cuts at: 10456 10839 11329 11338 11470 11557 11695 11940 12307
Size: 383 490 9 132 87 138 245 367
Cuts at: 12307 12346 12826 13051 13255 13428 13627 13938
Size: 39 480 225 204 173 199 311
Fragments arranged by size:
1108 911 745 659 615 512 490 480
474 383 383 367 338 330 330 325
324 319 311 298 276 271 250 245
237 237 225 213 204 199 173 145
138 132 129 126 126 126 105 87
84 75 62 52 51 50 47 42
39 30 18 14 12 9 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 800 1931 2008 4999 5845 6535 6712 8453
Size: 800 1131 77 2991 846 690 177 1741
Cuts at: 8453 9913 11590 11977 13938
Size: 1460 1677 387 1961
Fragments arranged by size:
2991 1961 1741 1677 1460 1131 846 800
690 387 177 77
EcoO109I rG'GnC_Cy
Cuts at: 0 408 1446 1494 2439 3981 5468 6670 6671
Size: 408 1038 48 945 1542 1487 1202 1
Cuts at: 6671 8024 8218 11583 11775 12168 13287 13938
Size: 1353 194 3365 192 393 1119 651
Fragments arranged by size:
3365 1542 1487 1353 1202 1119 1038 945
651 408 393 194 192 48 1
EcoRI G'AATT_C
Cuts at: 0 289 3421 9322 10792 11710 13938
Size: 289 3132 5901 1470 918 2228
Fragments arranged by size:
5901 3132 2228 1470 918 289
EcoRV GAT'ATC
Cuts at: 0 906 4668 7477 13938
Size: 906 3762 2809 6461
Fragments arranged by size:
6461 3762 2809 906
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1588 2020 3366 3844 4470 4927 8728 9859
Size: 1588 432 1346 478 626 457 3801 1131
Cuts at: 9859 10499 11604 13938
Size: 640 1105 2334
Fragments arranged by size:
3801 2334 1588 1346 1131 1105 640 626
478 457 432
FspI TGC'GCA
Cuts at: 0 103 196 7147 13345 13938
Size: 103 93 6951 6198 593
Fragments arranged by size:
6951 6198 593 103 93
HaeII r_GCGC'y
Cuts at: 0 145 166 182 243 927 1069 2505 3585
Size: 145 21 16 61 684 142 1436 1080
Cuts at: 3585 3910 6894 6996 7309 7464 7823 8428 9961
Size: 325 2984 102 313 155 359 605 1533
Cuts at: 9961 11565 12813 13119 13502 13652 13938
Size: 1604 1248 306 383 150 286
Fragments arranged by size:
2984 1604 1533 1436 1248 1080 684 605
383 359 325 313 306 286 155 150
145 142 102 61 21 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 784 816 1018 1050 1295 1327 1421 1453
Size: 784 32 202 32 245 32 94 32
Cuts at: 1453 1680 1712 1834 1866 2985 3017 4578 4610
Size: 227 32 122 32 1119 32 1561 32
Cuts at: 4610 4855 4887 5962 5994 6305 6337 7557 7589
Size: 245 32 1075 32 311 32 1220 32
Cuts at: 7589 7890 7922 8660 8692 9907 9939 10967 10999
Size: 301 32 738 32 1215 32 1028 32
Cuts at: 10999 11167 11199 11374 11406 11875 11907 12359 12391
Size: 168 32 175 32 469 32 452 32
Cuts at: 12391 12622 12654 13090 13122 13365 13397 13938
Size: 231 32 436 32 243 32 541
Fragments arranged by size:
1561 1220 1215 1119 1075 1028 784 738
541 469 452 436 311 301 245 245
243 231 227 202 175 168 122 94
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 747 2211 2700 2808 4311 8782 9636 10056
Size: 747 1464 489 108 1503 4471 854 420
Cuts at: 10056 12864 13629 13938
Size: 2808 765 309
Fragments arranged by size:
4471 2808 1503 1464 854 765 747 489
420 309 108
HindIII A'AGCT_T
Cuts at: 0 2704 4072 4113 13938
Size: 2704 1368 41 9825
Fragments arranged by size:
9825 2704 1368 41
HpaI GTT'AAC
Cuts at: 0 747 2211 13938
Size: 747 1464 11727
Fragments arranged by size:
11727 1464 747
KasI G'GCGC_C
Cuts at: 0 141 13115 13498 13648 13938
Size: 141 12974 383 150 290
Fragments arranged by size:
12974 383 290 150 141
KpnI G_GTAC'C
Cuts at: 0 3012 13938
Size: 3012 10926
MfeI C'AATT_G
Cuts at: 0 2921 5195 10187 12203 13938
Size: 2921 2274 4992 2016 1735
Fragments arranged by size:
4992 2921 2274 2016 1735
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3437 5981 6464 7310 8512 11074 12390 13025
Size: 3437 2544 483 846 1202 2562 1316 635
Cuts at: 13025 13133 13178 13463 13938
Size: 108 45 285 475
Fragments arranged by size:
3437 2562 2544 1316 1202 846 635 483
475 285 108 45
MscI TGG'CCA
Cuts at: 0 3578 4295 5140 5759 6037 6118 7060 7138
Size: 3578 717 845 619 278 81 942 78
Cuts at: 7138 9037 9508 9931 11951 12007 13938
Size: 1899 471 423 2020 56 1931
Fragments arranged by size:
3578 2020 1931 1899 942 845 717 619
471 423 278 81 78 56
MslI CAynn'nnrTG
Cuts at: 0 1790 1913 3514 6049 6515 6844 7114 7567
Size: 1790 123 1601 2535 466 329 270 453
Cuts at: 7567 7846 9085 10024 11368 12100 12433 12504 12597
Size: 279 1239 939 1344 732 333 71 93
Cuts at: 12597 12883 13522 13938
Size: 286 639 416
Fragments arranged by size:
2535 1790 1601 1344 1239 939 732 639
466 453 416 333 329 286 279 270
123 93 71
MspA1I CmG'CkG
Cuts at: 0 117 752 1821 1971 4107 4565 4698 5660
Size: 117 635 1069 150 2136 458 133 962
Cuts at: 5660 6465 7158 7185 7326 7455 7625 8724 9437
Size: 805 693 27 141 129 170 1099 713
Cuts at: 9437 9789 10399 10531 10761 11232 12259 12324 13464
Size: 352 610 132 230 471 1027 65 1140
Cuts at: 13464 13486 13598 13767 13938
Size: 22 112 169 171
Fragments arranged by size:
2136 1140 1099 1069 1027 962 805 713
693 635 610 471 458 352 230 171
170 169 150 141 133 132 129 117
112 65 27 22
NaeI GCC'GGC
Cuts at: 0 146 7519 13413 13938
Size: 146 7373 5894 525
Fragments arranged by size:
7373 5894 525 146
NarI GG'CG_CC
Cuts at: 0 142 13116 13499 13649 13938
Size: 142 12974 383 150 289
Fragments arranged by size:
12974 383 289 150 142
NcoI C'CATG_G
Cuts at: 0 8315 9926 11441 12008 12380 13938
Size: 8315 1611 1515 567 372 1558
Fragments arranged by size:
8315 1611 1558 1515 567 372
NdeI CA'TA_TG
Cuts at: 0 2493 6576 13938
Size: 2493 4083 7362
Fragments arranged by size:
7362 4083 2493
NgoMIV G'CCGG_C
Cuts at: 0 144 7517 13411 13938
Size: 144 7373 5894 527
Fragments arranged by size:
7373 5894 527 144
NheI G'CTAG_C
Cuts at: 0 9480 13938
Size: 9480 4458
NruI TCG'CGA
Cuts at: 0 1805 13938
Size: 1805 12133
NsiI A_TGCA'T
Cuts at: 0 1208 13938
Size: 1208 12730
NspI r_CATG'y
Cuts at: 0 1210 1247 9378 10116 10215 11625 12021 12163
Size: 1210 37 8131 738 99 1410 396 142
Cuts at: 12163 12358 12471 12857 13938
Size: 195 113 386 1081
Fragments arranged by size:
8131 1410 1210 1081 738 396 386 195
142 113 99 37
PasI CC'CwG_GG
Cuts at: 0 4049 12307 13938
Size: 4049 8258 1631
Fragments arranged by size:
8258 4049 1631
PciI A'CATG_T
Cuts at: 0 10112 10211 12017 12159 13938
Size: 10112 99 1806 142 1779
Fragments arranged by size:
10112 1806 1779 142 99
PflMI CCAn_nnn'nTGG
Cuts at: 0 3456 5247 5820 6821 7496 9528 9684 10001
Size: 3456 1791 573 1001 675 2032 156 317
Cuts at: 10001 10911 12090 13598 13938
Size: 910 1179 1508 340
Fragments arranged by size:
3456 2032 1791 1508 1179 1001 910 675
573 340 317 156
PfoI T'CCnGG_A
Cuts at: 0 863 3731 9011 9779 10841 10904 11495 12803
Size: 863 2868 5280 768 1062 63 591 1308
Cuts at: 12803 13938
Size: 1135
Fragments arranged by size:
5280 2868 1308 1135 1062 863 768 591
63
PmlI CAC'GTG
Cuts at: 0 2028 6846 8190 8754 12662 13524 13706 13938
Size: 2028 4818 1344 564 3908 862 182 232
Fragments arranged by size:
4818 3908 2028 1344 862 564 232 182
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 6931 6963 9196 9228 12799 12831 13938
Size: 6931 32 2233 32 3571 32 1107
Fragments arranged by size:
6931 3571 2233 1107 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 408 1446 1494 3981 5468 8024 11583 12168
Size: 408 1038 48 2487 1487 2556 3559 585
Cuts at: 12168 13287 13938
Size: 1119 651
Fragments arranged by size:
3559 2556 2487 1487 1119 1038 651 585
408 48
PshAI GACnn'nnGTC
Cuts at: 0 959 6263 12785 13938
Size: 959 5304 6522 1153
Fragments arranged by size:
6522 5304 1153 959
PsiI TTA'TAA
Cuts at: 0 8977 11575 13938
Size: 8977 2598 2363
Fragments arranged by size:
8977 2598 2363
PspOMI G'GGCC_C
Cuts at: 0 6670 8217 13938
Size: 6670 1547 5721
Fragments arranged by size:
6670 5721 1547
PspXI vC'TCGA_Gb
Cuts at: 0 6730 11615 13938
Size: 6730 4885 2323
Fragments arranged by size:
6730 4885 2323
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 6046 6078 13938
Size: 6046 32 7860
Fragments arranged by size:
7860 6046 32
PstI C_TGCA'G
Cuts at: 0 92 1379 1545 6887 7241 10068 10250 11334
Size: 92 1287 166 5342 354 2827 182 1084
Cuts at: 11334 11425 12222 12350 13469 13938
Size: 91 797 128 1119 469
Fragments arranged by size:
5342 2827 1287 1119 1084 797 469 354
182 166 128 92 91
PvuI CG_AT'CG
Cuts at: 0 11997 13938
Size: 11997 1941
PvuII CAG'CTG
Cuts at: 0 1821 1971 4107 4565 7326 7455 9437 9789
Size: 1821 150 2136 458 2761 129 1982 352
Cuts at: 9789 10399 10531 11232 12324 13464 13486 13598 13767
Size: 610 132 701 1092 1140 22 112 169
Cuts at: 13767 13938
Size: 171
Fragments arranged by size:
2761 2136 1982 1821 1140 1092 701 610
458 352 171 169 150 132 129 112
22
SacI G_AGCT'C
Cuts at: 0 3566 9149 13616 13938
Size: 3566 5583 4467 322
Fragments arranged by size:
5583 4467 3566 322
SalI G'TCGA_C
Cuts at: 0 9634 12862 13938
Size: 9634 3228 1076
Fragments arranged by size:
9634 3228 1076
SanDI GG'GwC_CC
Cuts at: 0 8024 13938
Size: 8024 5914
SapI GCTCTTCn'nnn_
Cuts at: 0 169 3941 6475 6682 6841 7378 10432 10436
Size: 169 3772 2534 207 159 537 3054 4
Cuts at: 10436 13938
Size: 3502
Fragments arranged by size:
3772 3502 3054 2534 537 207 169 159
4
ScaI AGT'ACT
Cuts at: 0 2166 11147 11479 13938
Size: 2166 8981 332 2459
Fragments arranged by size:
8981 2459 2166 332
SexAI A'CCwGG_T
Cuts at: 0 3011 9638 9908 10591 13938
Size: 3011 6627 270 683 3347
Fragments arranged by size:
6627 3347 3011 683 270
SfcI C'TryA_G
Cuts at: 0 88 1375 1541 6883 7237 10064 10246 10606
Size: 88 1287 166 5342 354 2827 182 360
Cuts at: 10606 11330 11421 12218 12346 13465 13938
Size: 724 91 797 128 1119 473
Fragments arranged by size:
5342 2827 1287 1119 797 724 473 360
354 182 166 128 91 88
SfoI GGC'GCC
Cuts at: 0 143 13117 13500 13650 13938
Size: 143 12974 383 150 288
Fragments arranged by size:
12974 383 288 150 143
SmaI CCC'GGG
Cuts at: 0 12 8216 11044 11083 13938
Size: 12 8204 2828 39 2855
Fragments arranged by size:
8204 2855 2828 39 12
SmlI C'TyrA_G
Cuts at: 0 1161 2197 6730 8786 9223 9663 11452 11615
Size: 1161 1036 4533 2056 437 440 1789 163
Cuts at: 11615 11724 13192 13938
Size: 109 1468 746
Fragments arranged by size:
4533 2056 1789 1468 1161 1036 746 440
437 163 109
SphI G_CATG'C
Cuts at: 0 1247 11625 12358 12857 13938
Size: 1247 10378 733 499 1081
Fragments arranged by size:
10378 1247 1081 733 499
SspI AAT'ATT
Cuts at: 0 597 2729 8774 13938
Size: 597 2132 6045 5164
Fragments arranged by size:
6045 5164 2132 597
StuI AGG'CCT
Cuts at: 0 1782 7393 8459 9122 13938
Size: 1782 5611 1066 663 4816
Fragments arranged by size:
5611 4816 1782 1066 663
StyI C'CwwG_G
Cuts at: 0 2004 2588 3899 4290 5684 6767 7055 7301
Size: 2004 584 1311 391 1394 1083 288 246
Cuts at: 7301 7649 8315 9926 10268 11441 12008 12036 12380
Size: 348 666 1611 342 1173 567 28 344
Cuts at: 12380 12395 12680 13502 13938
Size: 15 285 822 436
Fragments arranged by size:
2004 1611 1394 1311 1173 1083 822 666
584 567 436 391 348 344 342 288
285 246 28 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3007 4885 5608 5842 6253 6655 7023 7531
Size: 3007 1878 723 234 411 402 368 508
Cuts at: 7531 8088 8368 8394 11958 12267 12912 12958 13422
Size: 557 280 26 3564 309 645 46 464
Cuts at: 13422 13522 13938
Size: 100 416
Fragments arranged by size:
3564 3007 1878 723 645 557 508 464
416 411 402 368 309 280 234 100
46 26
TatI w'GTAC_w
Cuts at: 0 2164 2496 2976 3021 4059 4344 4788 6161
Size: 2164 332 480 45 1038 285 444 1373
Cuts at: 6161 7742 7919 8615 8897 11145 11477 12536 13938
Size: 1581 177 696 282 2248 332 1059 1402
Fragments arranged by size:
2248 2164 1581 1402 1373 1059 1038 696
480 444 332 332 285 282 177 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 35 67 1098 1130 2662 2694 2808 2840
Size: 35 32 1031 32 1532 32 114 32
Cuts at: 2840 6158 6190 6913 6945 8162 8194 11031 11063
Size: 3318 32 723 32 1217 32 2837 32
Cuts at: 11063 13938
Size: 2875
Fragments arranged by size:
3318 2875 2837 1532 1217 1031 723 114
35 32 32 32 32 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1309 5383 7720 11365 11388 13938
Size: 1309 4074 2337 3645 23 2550
Fragments arranged by size:
4074 3645 2550 2337 1309 23
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2406 3906 6649 12751 13005 13578 13938
Size: 2406 1500 2743 6102 254 573 360
Fragments arranged by size:
6102 2743 2406 1500 573 360 254
XhoI C'TCGA_G
Cuts at: 0 6730 11615 13938
Size: 6730 4885 2323
Fragments arranged by size:
6730 4885 2323
XmaI C'CCGG_G
Cuts at: 0 10 8214 11042 11081 13938
Size: 10 8204 2828 39 2857
Fragments arranged by size:
8204 2857 2828 39 10
XmnI GAAnn'nnTTC
Cuts at: 0 1012 1256 4114 4957 6103 6617 8569 10582
Size: 1012 244 2858 843 1146 514 1952 2013
Cuts at: 10582 10744 13938
Size: 162 3194
Fragments arranged by size:
3194 2858 2013 1952 1146 1012 843 514
244 162
ZraI GAC'GTC
Cuts at: 0 261 13938
Size: 261 13677
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspI PasI PciI PflMI PfoI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII SacI SalI SanDI SapI ScaI SexAI
SfcI SfoI SmaI SmlI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AgeI AscI AseI AsiSI AvrII BaeI BsiWI
BssHII BstZ17I FseI FspAI MluI NotI PacI PmeI
RsrII SacII SbfI SfiI SgfI SgrAI SnaBI SpeI
SrfI SwaI XbaI