(Circular) (Six-base) MAPSORT of: pFN21AE0136.seq Check: 7403 from: 1 to: 5611 pFN21AE0136 5611 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2107 2107 Size: 5611 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2370 3421 278 Size: 53 83 186 936 834 1051 2468 Fragments arranged by size: 2468 1051 936 834 186 83 53 AccI GT'mk_AC Cuts at: 1937 2954 1937 Size: 1017 4594 Acc65I G'GTAC_C Cuts at: 2932 2932 Size: 5611 AclI AA'CG_TT Cuts at: 1563 3738 4111 1563 Size: 2175 373 3063 Fragments arranged by size: 3063 2175 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2840 3674 5058 5326 1632 Size: 1208 834 1384 268 1917 Fragments arranged by size: 1917 1384 1208 834 268 AfeI AGC'GCT Cuts at: 2708 2708 Size: 5611 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5414 AflIII A'CryG_T Cuts at: 1203 2346 4510 5487 1203 Size: 1143 2164 977 1327 Fragments arranged by size: 2164 1327 1143 977 AgeI A'CCGG_T Cuts at: 4442 4442 Size: 5611 AhdI GACnn_n'nnGTC Cuts at: 4340 4340 Size: 5611 AleI CACnn'nnGTG Cuts at: 1166 2654 1166 Size: 1488 4123 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5611 AlwNI CAG_nnn'CTG Cuts at: 2089 2335 3354 4926 2089 Size: 246 1019 1572 2774 Fragments arranged by size: 2774 1572 1019 246 ApaI G_GGCC'C Cuts at: 2053 2247 2053 Size: 194 5417 ApaLI G'TGCA_C Cuts at: 3668 4824 3668 Size: 1156 4455 ApoI r'AATT_y Cuts at: 1493 2920 3146 3298 5407 1493 Size: 1427 226 152 2109 1697 Fragments arranged by size: 2109 1697 1427 226 152 AseI AT'TA_AT Cuts at: 160 1032 4165 160 Size: 872 3133 1606 Fragments arranged by size: 3133 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 AvaI C'yCGr_G Cuts at: 1870 1943 2376 2936 1870 Size: 73 433 560 4545 Fragments arranged by size: 4545 560 433 73 BamHI G'GATC_C Cuts at: 2941 3308 2941 Size: 367 5244 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2932 4387 618 Size: 325 217 627 1145 1455 1842 Fragments arranged by size: 1842 1455 1145 627 325 217 BanII G_rGCy'C Cuts at: 1391 2053 2247 2930 1391 Size: 662 194 683 4072 Fragments arranged by size: 4072 683 662 194 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4640 BbvCI CC'TCA_GC Cuts at: 2892 2892 Size: 5611 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3802 3836 1903 Size: 34 50 34 1781 34 3678 Fragments arranged by size: 3678 1781 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2133 3503 4713 2133 Size: 1370 1210 3031 Fragments arranged by size: 3031 1370 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5611 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 2105 4222 Size: 107 122 71 631 605 376 57 2117 Cuts at: 4222 136 Size: 1525 Fragments arranged by size: 2117 1525 631 605 376 122 107 71 57 BglII A'GATC_T Cuts at: 5606 5606 Size: 5611 BlpI GC'TnA_GC Cuts at: 2825 3028 2825 Size: 203 5408 Bme1580I G_kGCm'C Cuts at: 1790 2053 2247 2903 3672 4828 1790 Size: 263 194 656 769 1156 2573 Fragments arranged by size: 2573 1156 769 656 263 194 BmgBI CAC'GTC Cuts at: 1318 2533 1318 Size: 1215 4396 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2389 2635 3342 Size: 416 53 83 399 24 972 246 707 Cuts at: 3342 4300 442 Size: 958 1753 Fragments arranged by size: 1753 972 958 707 416 399 246 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5611 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2522 2618 4271 1239 Size: 1283 96 1653 2579 Fragments arranged by size: 2579 1653 1283 96 Bpu10I CC'TnA_GC Cuts at: 2258 2892 2258 Size: 634 4977 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3736 4601 4899 3736 Size: 865 298 4448 Fragments arranged by size: 4448 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2512 4274 882 Size: 617 1013 1762 2219 Fragments arranged by size: 2219 1762 1013 617 BsaAI yAC'GTr Cuts at: 493 1204 2347 3402 493 Size: 711 1143 1055 2702 Fragments arranged by size: 2702 1143 1055 711 BsaBI GATnn'nnATC Cuts at: 5605 5605 Size: 5611 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 3418 3800 4435 Size: 53 83 186 936 834 1051 382 635 Cuts at: 4435 275 Size: 1451 Fragments arranged by size: 1451 1051 936 834 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4044 4442 4716 4863 5274 4044 Size: 398 274 147 411 4381 Fragments arranged by size: 4381 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5386 Fragments arranged by size: 5386 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 2786 2789 1186 Size: 829 771 3 4008 Fragments arranged by size: 4008 829 771 3 BseYI C'CCAG_C Cuts at: 2082 2268 2272 2408 2423 2511 4814 5310 2082 Size: 186 4 136 15 88 2303 496 2383 Fragments arranged by size: 2383 2303 496 186 136 88 15 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2004 2429 2659 2681 2708 1163 Size: 573 268 425 230 22 27 4066 Fragments arranged by size: 4066 573 425 268 230 27 22 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3061 3822 4850 1540 Size: 275 186 1060 761 1028 2301 Fragments arranged by size: 2301 1060 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3672 3757 4828 3672 Size: 85 1071 4455 Fragments arranged by size: 4455 1071 85 BsmI GAATG_Cn' Cuts at: 3122 3215 3122 Size: 93 5518 BsmBI CGTCTCn'nnnn_ Cuts at: 2288 2288 Size: 5611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2247 2903 2930 3672 3757 4828 Size: 399 263 194 656 27 742 85 1071 Cuts at: 4828 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 656 399 263 194 85 27 BspEI T'CCGG_A Cuts at: 5274 5274 Size: 5611 BspHI T'CATG_A Cuts at: 3500 5230 3500 Size: 1730 3881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2107 2966 844 Size: 1263 859 3489 Fragments arranged by size: 3489 1263 859 BsrBI CCG'CTC Cuts at: 1394 1823 3498 1394 Size: 429 1675 3507 Fragments arranged by size: 3507 1675 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2419 4106 4280 65 Size: 1183 1171 1687 174 1396 Fragments arranged by size: 1687 1396 1183 1171 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4255 4442 1700 Size: 110 2445 187 2869 Fragments arranged by size: 2869 2445 187 110 BsrGI T'GTAC_A Cuts at: 96 2349 96 Size: 2253 3358 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5611 BssSI C'ACGA_G Cuts at: 2752 3671 4683 2752 Size: 919 1012 3680 Fragments arranged by size: 3680 1012 919 BstAPI GCAn_nnn'nTGC Cuts at: 794 2673 794 Size: 1879 3732 BstBI TT'CG_AA Cuts at: 4415 4415 Size: 5611 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5611 BstYI r'GATC_y Cuts at: 1972 2941 3308 3693 3710 5162 5248 5260 5606 Size: 969 367 385 17 1452 86 12 346 Cuts at: 5606 1972 Size: 1977 Fragments arranged by size: 1977 1452 969 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2457 2457 Size: 5611 BtgI C'CryG_G Cuts at: 513 1065 2805 513 Size: 552 1740 3319 Fragments arranged by size: 3319 1740 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4872 Fragments arranged by size: 4872 683 56 BtsI GCAGTG_nn' Cuts at: 2671 2727 3127 3921 3941 2671 Size: 56 400 794 20 4341 Fragments arranged by size: 4341 794 400 56 20 ClaI AT'CG_AT Cuts at: 1554 2227 1554 Size: 673 4938 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5576 DraI TTT'AAA Cuts at: 2915 2915 Size: 5611 DraIII CAC_nnn'GTG Cuts at: 2629 2629 Size: 5611 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4618 809 Size: 507 3302 1802 Fragments arranged by size: 3302 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2040 2097 2106 Size: 54 1425 50 275 18 210 57 9 Cuts at: 2106 3058 3947 5576 8 Size: 952 889 1629 43 Fragments arranged by size: 1629 1425 952 889 275 210 57 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3058 1537 Size: 275 1246 4090 Fragments arranged by size: 4090 1246 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2033 2191 3541 1353 Size: 680 158 1350 3423 Fragments arranged by size: 3423 1350 680 158 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4198 4572 4718 387 Size: 1064 138 2609 374 146 1280 Fragments arranged by size: 2609 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2522 2618 2840 3674 4271 5058 5326 Size: 393 890 96 222 834 597 787 268 Cuts at: 5326 1239 Size: 1524 Fragments arranged by size: 1524 890 834 787 597 393 268 222 96 EcoO109I rG'GnC_Cy Cuts at: 2049 2244 2332 2779 2049 Size: 195 88 447 4881 Fragments arranged by size: 4881 447 195 88 EcoRI G'AATT_C Cuts at: 2920 3298 2920 Size: 378 5233 FspI TGC'GCA Cuts at: 4117 4117 Size: 5611 HaeII r_GCGC'y Cuts at: 1678 2199 2587 2710 4758 1678 Size: 521 388 123 2048 2531 Fragments arranged by size: 2531 2048 521 388 123 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2131 2163 4252 4284 4326 Size: 32 135 32 559 32 2089 32 42 Cuts at: 4326 4358 1373 Size: 32 2626 Fragments arranged by size: 2626 2089 559 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2955 3201 669 Size: 988 281 1017 246 3079 Fragments arranged by size: 3079 1017 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5611 HpaI GTT'AAC Cuts at: 3201 3201 Size: 5611 KpnI G_GTAC'C Cuts at: 2936 2936 Size: 5611 MfeI C'AATT_G Cuts at: 3210 3210 Size: 5611 MluI A'CGCG_T Cuts at: 5487 5487 Size: 5611 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4725 4909 1305 Size: 3420 184 2007 Fragments arranged by size: 3420 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2042 2099 2108 10 Size: 54 1425 343 210 57 9 3513 Fragments arranged by size: 3513 1425 343 210 57 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 2162 2288 2654 3569 3928 4087 518 Size: 648 996 126 366 915 359 159 2042 Fragments arranged by size: 2042 996 915 648 366 359 159 126 MspA1I CmG'CkG Cuts at: 1606 2086 2272 2443 2896 3027 3704 4852 5097 Size: 480 186 171 453 131 677 1148 245 Cuts at: 5097 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 480 453 245 186 171 131 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5059 NdeI CA'TA_TG Cuts at: 387 387 Size: 5611 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5611 NotI GC'GGCC_GC Cuts at: 3058 3058 Size: 5611 NspI r_CATG'y Cuts at: 1132 1229 2969 4514 1132 Size: 97 1740 1545 2229 Fragments arranged by size: 2229 1740 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5611 PciI A'CATG_T Cuts at: 4510 4510 Size: 5611 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5611 PmeI GTTT'AAAC Cuts at: 2915 2915 Size: 5611 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3680 3712 5193 5225 3680 Size: 32 1481 32 4066 Fragments arranged by size: 4066 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2332 2332 Size: 5611 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5611 PsiI TTA'TAA Cuts at: 3181 3181 Size: 5611 PspOMI G'GGCC_C Cuts at: 2049 2243 2049 Size: 194 5417 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2471 2503 2471 Size: 32 5579 PstI C_TGCA'G Cuts at: 830 2172 2963 830 Size: 1342 791 3478 Fragments arranged by size: 3478 1342 791 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5611 PvuII CAG'CTG Cuts at: 2272 2896 2272 Size: 624 4987 SalI G'TCGA_C Cuts at: 1936 2953 1936 Size: 1017 4594 SbfI CC_TGCA'GG Cuts at: 2963 2963 Size: 5611 ScaI AGT'ACT Cuts at: 3859 5571 3859 Size: 1712 3899 SfcI C'TryA_G Cuts at: 826 1045 2168 2248 2959 4094 4775 4966 826 Size: 219 1123 80 711 1135 681 191 1471 Fragments arranged by size: 1471 1135 1123 711 681 219 191 80 SfiI GGCCn_nnn'nGGCC Cuts at: 2048 2105 2048 Size: 57 5554 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5611 SmaI CCC'GGG Cuts at: 1872 2378 2938 1872 Size: 506 560 4545 Fragments arranged by size: 4545 560 506 SmlI C'TyrA_G Cuts at: 820 1017 1943 3715 4616 4878 820 Size: 197 926 1772 901 262 1553 Fragments arranged by size: 1772 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3402 493 Size: 2909 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5611 SphI G_CATG'C Cuts at: 1132 2969 1132 Size: 1837 3774 SspI AAT'ATT Cuts at: 5 52 3535 5 Size: 47 3483 2081 Fragments arranged by size: 3483 2081 47 StuI AGG'CCT Cuts at: 1517 2057 1517 Size: 540 5071 StyI C'CwwG_G Cuts at: 513 1065 2100 3050 513 Size: 552 1035 950 3074 Fragments arranged by size: 3074 1035 950 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2304 2617 2684 3638 3655 3808 3993 Size: 1255 724 313 67 954 17 153 185 Cuts at: 3993 4438 325 Size: 445 1498 Fragments arranged by size: 1498 1255 954 724 445 313 185 153 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2304 2349 3857 Size: 274 80 33 51 1443 327 45 1508 Cuts at: 3857 5569 96 Size: 1712 138 Fragments arranged by size: 1712 1508 1443 327 274 138 80 51 45 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2013 2045 2826 2858 5571 5603 2013 Size: 32 781 32 2713 32 2021 Fragments arranged by size: 2713 2021 781 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2687 1316 Size: 1371 4240 XbaI T'CTAG_A Cuts at: 2947 2947 Size: 5611 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2431 2584 2622 2431 Size: 153 38 5420 Fragments arranged by size: 5420 153 38 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5611 XmaI C'CCGG_G Cuts at: 1870 2376 2936 1870 Size: 506 560 4545 Fragments arranged by size: 4545 560 506 XmnI GAAnn'nnTTC Cuts at: 3740 3740 Size: 5611 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2368 3419 276 Size: 53 83 186 936 834 1051 2468 Fragments arranged by size: 2468 1051 936 834 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI