(Circular) (Six-base) MAPSORT of: pFN21AE0511.seq Check: 5882 from: 1 to: 5632 pFN21AE0511 5632 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2915 2975 1937 Size: 978 60 4594 Fragments arranged by size: 4594 978 60 Acc65I G'GTAC_C Cuts at: 2953 2953 Size: 5632 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2556 2664 3695 5079 5347 1632 Size: 924 108 1031 1384 268 1917 Fragments arranged by size: 1917 1384 1031 924 268 108 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 2770 4531 5508 1203 Size: 1567 1761 977 1327 Fragments arranged by size: 1761 1567 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5632 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2058 2728 1184 Size: 874 670 4088 Fragments arranged by size: 4088 874 670 AlwNI CAG_nnn'CTG Cuts at: 3375 4947 3375 Size: 1572 4060 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2138 2301 2450 2752 2941 3167 3319 5428 Size: 645 163 149 302 189 226 152 2109 Cuts at: 5428 1493 Size: 1697 Fragments arranged by size: 2109 1697 645 302 226 189 163 152 149 AseI AT'TA_AT Cuts at: 160 1032 2601 4186 160 Size: 872 1569 1585 1606 Fragments arranged by size: 1606 1585 1569 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2957 1870 Size: 73 1014 4545 Fragments arranged by size: 4545 1014 73 BamHI G'GATC_C Cuts at: 2317 2962 3329 2317 Size: 645 367 4620 Fragments arranged by size: 4620 645 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2094 2953 4408 618 Size: 325 217 627 307 859 1455 1842 Fragments arranged by size: 1842 1455 859 627 325 307 217 BanII G_rGCy'C Cuts at: 1391 2951 1391 Size: 1560 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2349 928 Size: 971 450 4211 Fragments arranged by size: 4211 971 450 BbvCI CC'TCA_GC Cuts at: 2154 2154 Size: 5632 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3823 3857 1903 Size: 34 1886 34 3678 Fragments arranged by size: 3678 1886 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2402 3524 4734 2402 Size: 1122 1210 3300 Fragments arranged by size: 3300 1210 1122 BclI T'GATC_A Cuts at: 1548 2626 1548 Size: 1078 4554 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4243 136 Size: 107 122 71 631 605 2571 1525 Fragments arranged by size: 2571 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5627 5627 Size: 5632 BlpI GC'TnA_GC Cuts at: 3049 3049 Size: 5632 Bme1580I G_kGCm'C Cuts at: 1790 3693 4849 1790 Size: 1903 1156 2573 Fragments arranged by size: 2573 1903 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5632 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3363 4321 442 Size: 416 53 83 399 24 1946 958 1753 Fragments arranged by size: 1946 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2740 4292 1239 Size: 1501 1552 2579 Fragments arranged by size: 2579 1552 1501 Bpu10I CC'TnA_GC Cuts at: 2154 2154 Size: 5632 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2372 3757 4622 4920 2372 Size: 1385 865 298 3084 Fragments arranged by size: 3084 1385 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4295 882 Size: 617 2796 2219 Fragments arranged by size: 2796 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3423 493 Size: 711 2219 2702 Fragments arranged by size: 2702 2219 711 BsaBI GATnn'nnATC Cuts at: 2106 2476 5626 2106 Size: 370 3150 2112 Fragments arranged by size: 3150 2112 370 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3439 3821 4456 275 Size: 53 83 186 936 1906 382 635 1451 Fragments arranged by size: 1906 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4065 4463 4737 4884 5295 4065 Size: 398 274 147 411 4402 Fragments arranged by size: 4402 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2402 2432 1178 Size: 30 165 30 999 30 4378 Fragments arranged by size: 4378 999 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5632 BseYI C'CCAG_C Cuts at: 4835 5331 4835 Size: 496 5136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5059 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3082 3843 4871 1540 Size: 275 186 1081 761 1028 2301 Fragments arranged by size: 2301 1081 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3693 3778 4849 3693 Size: 85 1071 4476 Fragments arranged by size: 4476 1071 85 BsmI GAATG_Cn' Cuts at: 2717 3143 3236 2717 Size: 426 93 5113 Fragments arranged by size: 5113 426 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2951 3693 3778 4849 1391 Size: 399 1161 742 85 1071 2174 Fragments arranged by size: 2174 1161 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5295 5295 Size: 5632 BspHI T'CATG_A Cuts at: 2308 3521 5251 2308 Size: 1213 1730 2689 Fragments arranged by size: 2689 1730 1213 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2987 844 Size: 2143 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3519 1394 Size: 429 1696 3507 Fragments arranged by size: 3507 1696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4127 4301 65 Size: 1183 2879 174 1396 Fragments arranged by size: 2879 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4276 4463 1700 Size: 110 2466 187 2869 Fragments arranged by size: 2869 2466 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 3692 4704 3692 Size: 1012 4620 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5632 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 2821 1184 Size: 1637 3995 BstYI r'GATC_y Cuts at: 1972 2317 2836 2962 3329 3714 3731 5183 5269 Size: 345 519 126 367 385 17 1452 86 Cuts at: 5269 5281 5627 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 519 385 367 346 345 126 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2052 2052 Size: 5632 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4141 Fragments arranged by size: 4141 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4893 Fragments arranged by size: 4893 683 56 BtsI GCAGTG_nn' Cuts at: 2421 3148 3942 3962 2421 Size: 727 794 20 4091 Fragments arranged by size: 4091 794 727 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2851 2886 529 Size: 35 2287 35 3275 Fragments arranged by size: 3275 2287 35 35 DraI TTT'AAA Cuts at: 2296 2936 2296 Size: 640 4992 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4639 809 Size: 507 3323 1802 Fragments arranged by size: 3323 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2685 3079 3968 Size: 54 1425 50 275 18 855 394 889 Cuts at: 3968 5597 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 855 394 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3079 1537 Size: 275 1267 4090 Fragments arranged by size: 4090 1267 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2524 2688 3562 1353 Size: 1171 164 874 3423 Fragments arranged by size: 3423 1171 874 164 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4219 4593 4739 387 Size: 1064 138 2630 374 146 1280 Fragments arranged by size: 2630 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2556 2664 2740 3695 4292 5079 5347 Size: 393 924 108 76 955 597 787 268 Cuts at: 5347 1239 Size: 1524 Fragments arranged by size: 1524 955 924 787 597 393 268 108 76 EcoO109I rG'GnC_Cy Cuts at: 2356 2356 Size: 5632 EcoRI G'AATT_C Cuts at: 2941 3319 2941 Size: 378 5254 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2061 2102 2366 2061 Size: 41 264 5327 Fragments arranged by size: 5327 264 41 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 4779 1678 Size: 3101 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2188 4273 4305 4347 Size: 32 135 32 584 32 2085 32 42 Cuts at: 4347 4379 1373 Size: 32 2626 Fragments arranged by size: 2626 2085 584 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2661 2976 3222 669 Size: 988 281 723 315 246 3079 Fragments arranged by size: 3079 988 723 315 281 246 HindIII A'AGCT_T Cuts at: 748 2067 748 Size: 1319 4313 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KpnI G_GTAC'C Cuts at: 2957 2957 Size: 5632 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4746 4930 1305 Size: 3441 184 2007 Fragments arranged by size: 3441 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2687 10 Size: 54 1425 343 855 2955 Fragments arranged by size: 2955 1425 855 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2089 2244 3590 3949 4108 518 Size: 648 923 155 1346 359 159 2042 Fragments arranged by size: 2042 1346 923 648 359 159 155 MspA1I CmG'CkG Cuts at: 1606 2718 3048 3725 4873 5118 1606 Size: 1112 330 677 1148 245 2120 Fragments arranged by size: 2120 1148 1112 677 330 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4141 Fragments arranged by size: 4141 939 552 NdeI CA'TA_TG Cuts at: 387 2461 387 Size: 2074 3558 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NspI r_CATG'y Cuts at: 1132 1229 2774 2990 4535 1132 Size: 97 1545 216 1545 2229 Fragments arranged by size: 2229 1545 1545 216 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5632 PciI A'CATG_T Cuts at: 2770 4531 2770 Size: 1761 3871 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5632 PfoI T'CCnGG_A Cuts at: 2371 2371 Size: 5632 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3701 3733 5214 5246 3701 Size: 32 1481 32 4087 Fragments arranged by size: 4087 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2356 2356 Size: 5632 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 3202 3202 Size: 5632 PstI C_TGCA'G Cuts at: 830 2984 830 Size: 2154 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 PvuII CAG'CTG Cuts at: 2718 2718 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 3880 5592 3880 Size: 1712 3920 SfcI C'TryA_G Cuts at: 826 1045 2980 4115 4796 4987 826 Size: 219 1935 1135 681 191 1471 Fragments arranged by size: 1935 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2959 1872 Size: 1087 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2351 3736 4637 4899 820 Size: 197 926 408 1385 901 262 1553 Fragments arranged by size: 1553 1385 926 901 408 262 197 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2990 1132 Size: 1858 3774 SspI AAT'ATT Cuts at: 5 52 2599 3556 5 Size: 47 2547 957 2081 Fragments arranged by size: 2547 2081 957 47 StuI AGG'CCT Cuts at: 1517 2505 1517 Size: 988 4644 StyI C'CwwG_G Cuts at: 513 1065 2004 3071 513 Size: 552 939 1067 3074 Fragments arranged by size: 3074 1067 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3659 3676 3829 4014 4459 325 Size: 1255 2079 17 153 185 445 1498 Fragments arranged by size: 2079 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3878 5590 96 Size: 274 80 33 51 1443 1901 1712 138 Fragments arranged by size: 1901 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5592 5624 5592 Size: 32 5600 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2968 2968 Size: 5632 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2957 1870 Size: 1087 4545 XmnI GAAnn'nnTTC Cuts at: 2304 3761 2304 Size: 1457 4175 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI