(Circular) (Six-base) MAPSORT of: pFN21AB6496.seq Check: 9925 from: 1 to: 5779 pFN21AB6496 5779 bp With 182 enzymes: SgfI * October 20, 2009 10:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3589 278 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3057 3122 1937 Size: 1120 65 4594 Fragments arranged by size: 4594 1120 65 Acc65I G'GTAC_C Cuts at: 3100 3100 Size: 5779 AclI AA'CG_TT Cuts at: 1563 3906 4279 1563 Size: 2343 373 3063 Fragments arranged by size: 3063 2343 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3842 5226 5494 1632 Size: 2210 1384 268 1917 Fragments arranged by size: 2210 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5582 AflIII A'CryG_T Cuts at: 1203 4678 5655 1203 Size: 3475 977 1327 Fragments arranged by size: 3475 1327 977 AgeI A'CCGG_T Cuts at: 4610 4610 Size: 5779 AhdI GACnn_n'nnGTC Cuts at: 4508 4508 Size: 5779 AleI CACnn'nnGTG Cuts at: 1166 2786 1166 Size: 1620 4159 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5779 AlwNI CAG_nnn'CTG Cuts at: 2020 2212 3031 3522 5094 2020 Size: 192 819 491 1572 2705 Fragments arranged by size: 2705 1572 819 491 192 ApaI G_GGCC'C Cuts at: 2549 2549 Size: 5779 ApaLI G'TGCA_C Cuts at: 3836 4992 3836 Size: 1156 4623 ApoI r'AATT_y Cuts at: 1493 2201 2449 3088 3314 3466 5575 1493 Size: 708 248 639 226 152 2109 1697 Fragments arranged by size: 2109 1697 708 639 248 226 152 AseI AT'TA_AT Cuts at: 160 1032 4333 160 Size: 872 3301 1606 Fragments arranged by size: 3301 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 AvaI C'yCGr_G Cuts at: 1870 1943 2868 3104 1870 Size: 73 925 236 4545 Fragments arranged by size: 4545 925 236 73 BamHI G'GATC_C Cuts at: 3109 3476 3109 Size: 367 5412 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2098 3100 4555 618 Size: 325 217 627 311 1002 1455 1842 Fragments arranged by size: 1842 1455 1002 627 325 311 217 BanII G_rGCy'C Cuts at: 1391 2306 2549 2922 2954 3098 1391 Size: 915 243 373 32 144 4072 Fragments arranged by size: 4072 915 373 243 144 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4808 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3970 4004 1903 Size: 34 2033 34 3678 Fragments arranged by size: 3678 2033 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3671 4881 3671 Size: 1210 4569 BclI T'GATC_A Cuts at: 1548 1548 Size: 5779 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4390 136 Size: 107 122 71 631 605 2718 1525 Fragments arranged by size: 2718 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2088 2413 5774 2088 Size: 325 3361 2093 Fragments arranged by size: 3361 2093 325 BlpI GC'TnA_GC Cuts at: 3196 3196 Size: 5779 Bme1580I G_kGCm'C Cuts at: 1790 2549 3840 4996 1790 Size: 759 1291 1156 2573 Fragments arranged by size: 2573 1291 1156 759 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5779 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3510 4468 442 Size: 416 53 83 399 24 2093 958 1753 Fragments arranged by size: 2093 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2733 4439 1239 Size: 1494 1706 2579 Fragments arranged by size: 2579 1706 1494 Bpu10I CC'TnA_GC Cuts at: 2021 2021 Size: 5779 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3904 4769 5067 3904 Size: 865 298 4616 Fragments arranged by size: 4616 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4442 882 Size: 617 2943 2219 Fragments arranged by size: 2943 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2698 3570 493 Size: 711 1494 872 2702 Fragments arranged by size: 2702 1494 872 711 BsaBI GATnn'nnATC Cuts at: 2295 5773 2295 Size: 3478 2301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3586 3968 4603 275 Size: 53 83 186 936 2053 382 635 1451 Fragments arranged by size: 2053 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2169 4212 4610 4884 5031 5442 2169 Size: 2043 398 274 147 411 2506 Fragments arranged by size: 2506 2043 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5554 Fragments arranged by size: 5554 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2781 1186 Size: 1595 4184 BseYI C'CCAG_C Cuts at: 2538 2542 4982 5478 2538 Size: 4 2440 496 2839 Fragments arranged by size: 2839 2440 496 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3068 1163 Size: 573 1332 3874 Fragments arranged by size: 3874 1332 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2170 3229 3990 5018 1540 Size: 275 186 169 1059 761 1028 2301 Fragments arranged by size: 2301 1059 1028 761 275 186 169 BsiHKAI G_wGCw'C Cuts at: 2027 2306 3840 3925 4996 2027 Size: 279 1534 85 1071 2810 Fragments arranged by size: 2810 1534 1071 279 85 BsmI GAATG_Cn' Cuts at: 2594 3290 3383 2594 Size: 696 93 4990 Fragments arranged by size: 4990 696 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2649 2649 Size: 5779 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2306 2549 2922 2954 3098 3840 Size: 399 237 279 243 373 32 144 742 Cuts at: 3840 3925 4996 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 373 279 243 237 144 85 32 BspEI T'CCGG_A Cuts at: 5442 5442 Size: 5779 BspHI T'CATG_A Cuts at: 3668 5398 3668 Size: 1730 4049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3134 844 Size: 2290 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3666 1394 Size: 429 1843 3507 Fragments arranged by size: 3507 1843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4274 4448 65 Size: 1183 3026 174 1396 Fragments arranged by size: 3026 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4423 4610 1700 Size: 110 2613 187 2869 Fragments arranged by size: 2869 2613 187 110 BsrGI T'GTAC_A Cuts at: 96 2778 96 Size: 2682 3097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5779 BssSI C'ACGA_G Cuts at: 3839 4851 3839 Size: 1012 4767 BstAPI GCAn_nnn'nTGC Cuts at: 794 2031 2527 794 Size: 1237 496 4046 Fragments arranged by size: 4046 1237 496 BstBI TT'CG_AA Cuts at: 4583 4583 Size: 5779 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5779 BstXI CCAn_nnnn'nTGG Cuts at: 2542 2542 Size: 5779 BstYI r'GATC_y Cuts at: 1972 2088 2209 2413 3109 3476 3861 3878 5330 Size: 116 121 204 696 367 385 17 1452 Cuts at: 5330 5416 5428 5774 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 696 385 367 346 204 121 116 86 17 12 BstZ17I GTA'TAC Cuts at: 3058 3058 Size: 5779 BtgI C'CryG_G Cuts at: 513 1065 2004 2308 513 Size: 552 939 304 3984 Fragments arranged by size: 3984 939 552 304 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5040 Fragments arranged by size: 5040 683 56 BtsI GCAGTG_nn' Cuts at: 3295 4089 4109 3295 Size: 794 20 4965 Fragments arranged by size: 4965 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5779 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5744 DraI TTT'AAA Cuts at: 3083 3083 Size: 5779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4786 809 Size: 507 3470 1802 Fragments arranged by size: 3470 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3226 4115 5744 Size: 54 1425 50 275 18 1396 889 1629 Cuts at: 5744 8 Size: 43 Fragments arranged by size: 1629 1425 1396 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3226 1537 Size: 275 1414 4090 Fragments arranged by size: 4090 1414 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3709 1353 Size: 2356 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4366 4740 4886 387 Size: 1064 138 2777 374 146 1280 Fragments arranged by size: 2777 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2304 2304 Size: 5779 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2733 3842 4439 5226 5494 1239 Size: 393 1101 1109 597 787 268 1524 Fragments arranged by size: 1524 1109 1101 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2546 2546 Size: 5779 EcoRI G'AATT_C Cuts at: 3088 3466 3088 Size: 378 5401 FspI TGC'GCA Cuts at: 4285 4285 Size: 5779 HaeII r_GCGC'y Cuts at: 1678 2557 4926 1678 Size: 879 2369 2531 Fragments arranged by size: 2531 2369 879 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2219 2251 3003 3035 4420 Size: 32 135 32 647 32 752 32 1385 Cuts at: 4420 4452 4494 4526 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1385 752 647 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2997 3123 3369 669 Size: 988 281 1059 126 246 3079 Fragments arranged by size: 3079 1059 988 281 246 126 HindIII A'AGCT_T Cuts at: 748 748 Size: 5779 HpaI GTT'AAC Cuts at: 2997 3369 2997 Size: 372 5407 KpnI G_GTAC'C Cuts at: 3104 3104 Size: 5779 MfeI C'AATT_G Cuts at: 2512 3378 2512 Size: 866 4913 MluI A'CGCG_T Cuts at: 5655 5655 Size: 5779 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4893 5077 1305 Size: 3588 184 2007 Fragments arranged by size: 3588 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3957 Fragments arranged by size: 3957 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2700 2786 2844 3737 4096 4255 518 Size: 648 1534 86 58 893 359 159 2042 Fragments arranged by size: 2042 1534 893 648 359 159 86 58 MspA1I CmG'CkG Cuts at: 1606 2542 3195 3872 5020 5265 1606 Size: 936 653 677 1148 245 2120 Fragments arranged by size: 2120 1148 936 677 653 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2308 513 Size: 552 939 304 3984 Fragments arranged by size: 3984 939 552 304 NdeI CA'TA_TG Cuts at: 387 387 Size: 5779 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5779 NotI GC'GGCC_GC Cuts at: 3226 3226 Size: 5779 NspI r_CATG'y Cuts at: 1132 1229 3137 4682 1132 Size: 97 1908 1545 2229 Fragments arranged by size: 2229 1908 1545 97 PasI CC'CwG_GG Cuts at: 1348 2549 1348 Size: 1201 4578 PciI A'CATG_T Cuts at: 4678 4678 Size: 5779 PflMI CCAn_nnn'nTGG Cuts at: 1108 2212 2575 1108 Size: 1104 363 4312 Fragments arranged by size: 4312 1104 363 PfoI T'CCnGG_A Cuts at: 2205 2205 Size: 5779 PmeI GTTT'AAAC Cuts at: 3083 3083 Size: 5779 PmlI CAC'GTG Cuts at: 2698 2698 Size: 5779 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3848 3880 5361 5393 3848 Size: 32 1481 32 4234 Fragments arranged by size: 4234 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5779 PsiI TTA'TAA Cuts at: 3349 3349 Size: 5779 PspOMI G'GGCC_C Cuts at: 2545 2545 Size: 5779 PstI C_TGCA'G Cuts at: 830 2157 3131 830 Size: 1327 974 3478 Fragments arranged by size: 3478 1327 974 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5779 PvuII CAG'CTG Cuts at: 2542 2542 Size: 5779 SacI G_AGCT'C Cuts at: 2306 2306 Size: 5779 SalI G'TCGA_C Cuts at: 1936 3121 1936 Size: 1185 4594 SbfI CC_TGCA'GG Cuts at: 3131 3131 Size: 5779 ScaI AGT'ACT Cuts at: 4027 5739 4027 Size: 1712 4067 SfcI C'TryA_G Cuts at: 826 1045 2137 2153 3127 4262 4943 5134 826 Size: 219 1092 16 974 1135 681 191 1471 Fragments arranged by size: 1471 1135 1092 974 681 219 191 16 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5779 SmaI CCC'GGG Cuts at: 1872 2870 3106 1872 Size: 998 236 4545 Fragments arranged by size: 4545 998 236 SmlI C'TyrA_G Cuts at: 820 1017 1943 3883 4784 5046 820 Size: 197 926 1940 901 262 1553 Fragments arranged by size: 1940 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3570 493 Size: 3077 2702 SpeI A'CTAG_T Cuts at: 152 2605 152 Size: 2453 3326 SphI G_CATG'C Cuts at: 1132 3137 1132 Size: 2005 3774 SspI AAT'ATT Cuts at: 5 52 2386 2754 3703 5 Size: 47 2334 368 949 2081 Fragments arranged by size: 2334 2081 949 368 47 StuI AGG'CCT Cuts at: 1517 2135 1517 Size: 618 5161 StyI C'CwwG_G Cuts at: 513 1065 2004 2308 3218 513 Size: 552 939 304 910 3074 Fragments arranged by size: 3074 939 910 552 304 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2204 2552 2763 3806 3823 3976 4161 Size: 1255 624 348 211 1043 17 153 185 Cuts at: 4161 4606 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1043 624 445 348 211 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2778 4025 5737 Size: 274 80 33 51 1443 801 1247 1712 Cuts at: 5737 96 Size: 138 Fragments arranged by size: 1712 1443 1247 801 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2439 2471 5739 5771 2439 Size: 32 3268 32 2447 Fragments arranged by size: 3268 2447 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5779 XbaI T'CTAG_A Cuts at: 2416 3115 2416 Size: 699 5080 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2546 2546 Size: 5779 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5779 XmaI C'CCGG_G Cuts at: 1870 2868 3104 1870 Size: 998 236 4545 Fragments arranged by size: 4545 998 236 XmnI GAAnn'nnTTC Cuts at: 3908 3908 Size: 5779 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3587 276 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI