################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccI AclI AgeI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbeI BbvCI BcgI BciVI BlnI BpmI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccIII 1016 TCCGGA T^CCGGA AcsI 977 AAATTT [GA]^AATT[CT] AcyI 1318 GGCGTC G[GA]^CG[CT]C AflII 1420 CTTAAG C^TTAAG AhdI 191 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 622 CCTCAGG CC^T[ACGT]AGG ApaI 77 GGGCCC GGGCC^C ApoI 977 AAATTT [GA]^AATT[CT] AspEI 191 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 12 GTGCTC G[AT]GC[AT]^C BanI 739 GGCACC G^G[CT][GA]CC BbsI 147 GTCTTC GAAGAC(2/6) BclI 615 TGATCA T^GATCA BfaI 1376 CTAG C^TAG BfrI 1420 CTTAAG C^TTAAG BglI 259 GCCCCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 563 AGATCT A^GATCT BpuAI 147 GTCTTC GAAGAC(2/6) BsaBI 277 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1318 GGCGTC G[GA]^CG[CT]C BsaWI 1016 TCCGGA [AT]^CCGG[AT] BseAI 1016 TCCGGA T^CCGGA BsiEI 82 CGGCCG CG[GA][CT]^CG BsiHKAI 12 GTGCTC G[AT]GC[AT]^C BsmBI 1108 GAGACG CGTCTC(1/5) BspEI 1016 TCCGGA T^CCGGA BsrDI 754 CATTGC GCAATG(2/0) BsrI 518 ACTGG ACTGG(1/-1) BssSI 252 CACGAG CACGAG(-5/-1) BstXI 759 CCACTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 563 AGATCT [GA]^GATC[CT] Bsu36I 622 CCTCAGG CC^T[ACGT]AGG EagI 82 CGGCCG C^GGCCG Eam1105I 191 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 82 CGGCCG C^GGCCG Eco52I 82 CGGCCG C^GGCCG Eco81I 622 CCTCAGG CC^T[ACGT]AGG FbaI 615 TGATCA T^GATCA Hin1I 1318 GGCGTC G[GA]^CG[CT]C MaeI 1376 CTAG C^TAG MamI 277 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 563 AGATCT [GA]^GATC[CT] MroI 1016 TCCGGA T^CCGGA NcoI 1349 CCATGG C^CATGG PflMI 17 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 43 GGGACCC [GA]G^G[AT]CC[CT] PvuII 409 CAGCTG CAG^CTG SapI 1069 GCTCTTC GCTCTTC(1/4) SbfI 925 CCTGCAGG CCTGCA^GG SexAI 192 ACCTGGT A^CC[AT]GGT SmlI 1420 CTTAAG C^T[CT][GA]AG Sse8387I 925 CCTGCAGG CCTGCA^GG Tsp509I 978 AATT ^AATT TspRI 534 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 17 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 89 CCAGGGAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 563 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AlwNI 1096 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1261 CAGAGCCTG Aor51HI 174 AGCGCT AGC^GCT 710 AGCGCT AvaI 170 CCCGAG C^[CT]CG[GA]G 544 CCCGAG AvaII 44 GGACC G^G[AT]CC 540 GGACC BalI 266 TGGCCA TGG^CCA 942 TGGCCA BbrPI 702 CACGTG CAC^GTG 819 CACGTG BlpI 114 GCTGAGC GC^T[ACGT]AGC 126 GCTGAGC Bpu1102I 114 GCTGAGC GC^T[ACGT]AGC 126 GCTGAGC BsaAI 702 CACGTG [CT]AC^GT[GA] 819 CACGTG BsaI 189 GAGACC GGTCTC(1/5) 1205 GAGACC BseRI 547 GAGGAG GAGGAG(10/8) 1399 GAGGAG BsgI 748 GTGCAG GTGCAG(16/14) 997 GTGCAG BsoBI 170 CCCGAG C^[CT]CG[GA]G 544 CCCGAG BsrFI 57 GCCGGC [GA]^CCGG[CT] 1253 GCCGGC CelII 114 GCTGAGC GC^T[ACGT]AGC 126 GCTGAGC Cfr10I 57 GCCGGC [GA]^CCGG[CT] 1253 GCCGGC Eco47III 174 AGCGCT AGC^GCT 710 AGCGCT FauI 48 CCCGC CCCGC(4/6) 136 CCCGC HaeII 174 AGCGCT [GA]GCGC^[CT] 710 AGCGCT HincII 642 GTTGAC GT[CT]^[GA]AC 670 GTCAAC HindII 642 GTTGAC GT[CT]^[GA]AC 670 GTCAAC KspI 49 CCGCGG CCGC^GG 840 CCGCGG MaeIII 1120 GTGAC ^GT[ACGT]AC 1321 GTCAC MluNI 266 TGGCCA TGG^CCA 942 TGGCCA MscI 266 TGGCCA TGG^CCA 942 TGGCCA MseI 1187 TTAA T^TAA 1421 TTAA NaeI 57 GCCGGC GCC^GGC 1253 GCCGGC NgoMIV 57 GCCGGC G^CCGGC 1253 GCCGGC PleI 1137 GACTC GAGTC(4/5) 932 GAGTC PmaCI 702 CACGTG CAC^GTG 819 CACGTG PmlI 702 CACGTG CAC^GTG 819 CACGTG SacII 49 CCGCGG CCGC^GG 840 CCGCGG TfiI 1155 GATTC G^A[AT]TC 1406 GATTC Tru9I 1187 TTAA T^TAA 1421 TTAA Tsp45I 1120 GTGAC ^GT[GC]AC 1321 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI TthHB8I