(Circular) (Six-base) MAPSORT of: pFN21AB5686.seq Check: 5528 from: 1 to: 6145 pFN21AB5686 6145 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3955 278 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3488 1937 Size: 1551 4594 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4208 5592 5860 1632 Size: 2576 1384 268 1917 Fragments arranged by size: 2576 1917 1384 268 AfeI AGC'GCT Cuts at: 2181 2717 2181 Size: 536 5609 AflII C'TTAA_G Cuts at: 820 1017 3425 820 Size: 197 2408 3540 Fragments arranged by size: 3540 2408 197 AflIII A'CryG_T Cuts at: 1203 2706 2708 2825 5044 6021 1203 Size: 1503 2 117 2219 977 1327 Fragments arranged by size: 2219 1503 1327 977 117 2 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 2201 4874 2201 Size: 2673 3472 AleI CACnn'nnGTG Cuts at: 1166 2259 2750 2769 1166 Size: 1093 491 19 4542 Fragments arranged by size: 4542 1093 491 19 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2484 2768 1184 Size: 1300 284 4561 Fragments arranged by size: 4561 1300 284 AlwNI CAG_nnn'CTG Cuts at: 3106 3271 3888 5460 3106 Size: 165 617 1572 3791 Fragments arranged by size: 3791 1572 617 165 ApaI G_GGCC'C Cuts at: 2086 2086 Size: 6145 ApaLI G'TGCA_C Cuts at: 4202 5358 4202 Size: 1156 4989 ApoI r'AATT_y Cuts at: 1493 2982 3454 3680 3832 5941 1493 Size: 1489 472 226 152 2109 1697 Fragments arranged by size: 2109 1697 1489 472 226 152 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2175 2549 3470 1870 Size: 73 232 374 921 4545 Fragments arranged by size: 4545 921 374 232 73 BamHI G'GATC_C Cuts at: 3475 3842 3475 Size: 367 5778 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2744 3466 4921 618 Size: 325 217 627 957 722 1455 1842 Fragments arranged by size: 1842 1455 957 722 627 325 217 BanII G_rGCy'C Cuts at: 1391 2086 2742 3100 3464 1391 Size: 695 656 358 364 4072 Fragments arranged by size: 4072 695 656 364 358 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2145 928 Size: 971 246 4928 Fragments arranged by size: 4928 971 246 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4336 4370 1903 Size: 34 2399 34 3678 Fragments arranged by size: 3678 2399 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5247 4037 Size: 1210 4935 BclI T'GATC_A Cuts at: 1548 2620 1548 Size: 1072 5073 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2270 4756 136 Size: 107 122 71 631 605 598 2486 1525 Fragments arranged by size: 2486 1525 631 605 598 122 107 71 BglII A'GATC_T Cuts at: 2568 6140 2568 Size: 3572 2573 BlpI GC'TnA_GC Cuts at: 2120 2132 3562 2120 Size: 12 1430 4703 Fragments arranged by size: 4703 1430 12 Bme1580I G_kGCm'C Cuts at: 1790 2086 2266 4206 5362 1790 Size: 296 180 1940 1156 2573 Fragments arranged by size: 2573 1940 1156 296 180 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6145 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3876 4834 442 Size: 416 53 83 399 24 2459 958 1753 Fragments arranged by size: 2459 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2425 2425 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4805 1239 Size: 3566 2579 Bpu10I CC'TnA_GC Cuts at: 3069 3278 3069 Size: 209 5936 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4270 5135 5433 4270 Size: 865 298 4982 Fragments arranged by size: 4982 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2188 3204 4808 882 Size: 617 689 1016 1604 2219 Fragments arranged by size: 2219 1604 1016 689 617 BsaAI yAC'GTr Cuts at: 493 1204 2709 2826 3936 493 Size: 711 1505 117 1110 2702 Fragments arranged by size: 2702 1505 1110 711 117 BsaBI GATnn'nnATC Cuts at: 2286 6139 2286 Size: 3853 2292 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3324 3952 4334 4969 Size: 53 83 186 936 1791 628 382 635 Cuts at: 4969 275 Size: 1451 Fragments arranged by size: 1791 1451 936 635 628 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3021 4578 4976 5250 5397 5808 3021 Size: 1557 398 274 147 411 3358 Fragments arranged by size: 3358 1557 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2824 2854 3029 3059 1178 Size: 30 165 30 1421 30 175 30 4264 Fragments arranged by size: 4264 1421 175 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2567 3419 1186 Size: 1381 852 3912 Fragments arranged by size: 3912 1381 852 BseYI C'CCAG_C Cuts at: 2412 2511 3392 5348 5844 2412 Size: 99 881 1956 496 2713 Fragments arranged by size: 2713 1956 881 496 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2774 3023 1163 Size: 573 1038 249 4285 Fragments arranged by size: 4285 1038 573 249 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2090 3595 4356 5384 1540 Size: 275 186 89 1505 761 1028 2301 Fragments arranged by size: 2301 1505 1028 761 275 186 89 BsiHKAI G_wGCw'C Cuts at: 2021 4206 4291 5362 2021 Size: 2185 85 1071 2804 Fragments arranged by size: 2804 2185 1071 85 BsmI GAATG_Cn' Cuts at: 3656 3749 3656 Size: 93 6052 BsmBI CGTCTCn'nnnn_ Cuts at: 3107 3107 Size: 6145 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2086 2266 2742 3100 3464 4206 Size: 399 231 65 180 476 358 364 742 Cuts at: 4206 4291 5362 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 476 399 364 358 231 180 85 65 BspEI T'CCGG_A Cuts at: 3021 5808 3021 Size: 2787 3358 BspHI T'CATG_A Cuts at: 4034 5764 4034 Size: 1730 4415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3500 844 Size: 2656 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4032 1394 Size: 429 2209 3507 Fragments arranged by size: 3507 2209 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2758 4640 4814 65 Size: 1183 1510 1882 174 1396 Fragments arranged by size: 1882 1510 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2062 3258 4789 4976 1700 Size: 110 252 1196 1531 187 2869 Fragments arranged by size: 2869 1531 1196 252 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6145 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6145 BssSI C'ACGA_G Cuts at: 2257 4205 5217 2257 Size: 1948 1012 3185 Fragments arranged by size: 3185 1948 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6145 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6145 BstXI CCAn_nnnn'nTGG Cuts at: 2771 2771 Size: 6145 BstYI r'GATC_y Cuts at: 1972 2568 3475 3842 4227 4244 5696 5782 5794 Size: 596 907 367 385 17 1452 86 12 Cuts at: 5794 6140 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 907 596 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2628 2628 Size: 6145 BtgI C'CryG_G Cuts at: 513 1065 2004 2054 2845 3354 513 Size: 552 939 50 791 509 3304 Fragments arranged by size: 3304 939 791 552 509 50 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5406 Fragments arranged by size: 5406 683 56 BtsI GCAGTG_nn' Cuts at: 3661 4455 4475 3661 Size: 794 20 5331 Fragments arranged by size: 5331 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6110 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2087 2271 2842 Size: 54 1425 50 275 18 257 184 571 Cuts at: 2842 2947 3592 4481 6110 8 Size: 105 645 889 1629 43 Fragments arranged by size: 1629 1425 889 645 571 275 257 184 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2087 3592 1537 Size: 275 275 1505 4090 Fragments arranged by size: 4090 1505 275 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2447 2596 3081 4075 1353 Size: 1094 149 485 994 3423 Fragments arranged by size: 3423 1094 994 485 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4732 5106 5252 387 Size: 1064 138 3143 374 146 1280 Fragments arranged by size: 3143 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4208 4805 5592 5860 1239 Size: 393 2576 597 787 268 1524 Fragments arranged by size: 2576 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2049 2082 2534 2804 3103 2049 Size: 33 452 270 299 5091 Fragments arranged by size: 5091 452 299 270 33 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 FspI TGC'GCA Cuts at: 4651 4651 Size: 6145 HaeII r_GCGC'y Cuts at: 1678 2183 2719 5292 1678 Size: 505 536 2573 2531 Fragments arranged by size: 2573 2531 536 505 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2187 2219 2570 2602 2641 Size: 32 135 32 615 32 351 32 39 Cuts at: 2641 2673 2904 2936 3029 3061 4786 4818 4860 Size: 32 231 32 93 32 1725 32 42 Cuts at: 4860 4892 1373 Size: 32 2626 Fragments arranged by size: 2626 1725 615 351 231 135 93 42 39 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2649 2677 3489 3735 669 Size: 988 281 711 28 812 246 3079 Fragments arranged by size: 3079 988 812 711 281 246 28 HindIII A'AGCT_T Cuts at: 748 748 Size: 6145 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3072 5259 5443 1305 Size: 1767 2187 184 2007 Fragments arranged by size: 2187 2007 1767 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2273 2949 10 Size: 54 1425 343 441 676 3206 Fragments arranged by size: 3206 1425 676 441 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2259 2750 2769 4103 4462 4621 518 Size: 648 1093 491 19 1334 359 159 2042 Fragments arranged by size: 2042 1334 1093 648 491 359 159 19 MspA1I CmG'CkG Cuts at: 1606 2056 2416 2505 2847 3561 4238 5386 5631 Size: 450 360 89 342 714 677 1148 245 Cuts at: 5631 1606 Size: 2120 Fragments arranged by size: 2120 1148 714 677 450 360 342 245 89 NaeI GCC'GGC Cuts at: 2064 3260 2064 Size: 1196 4949 NcoI C'CATG_G Cuts at: 513 1065 2004 3354 513 Size: 552 939 1350 3304 Fragments arranged by size: 3304 1350 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NgoMIV G'CCGG_C Cuts at: 2062 3258 2062 Size: 1196 4949 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 3592 3592 Size: 6145 NspI r_CATG'y Cuts at: 1132 1229 3503 5048 1132 Size: 97 2274 1545 2229 Fragments arranged by size: 2274 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2094 3100 3137 1348 Size: 746 1006 37 4356 Fragments arranged by size: 4356 1006 746 37 PciI A'CATG_T Cuts at: 5044 5044 Size: 6145 PflMI CCAn_nnn'nTGG Cuts at: 1108 2028 1108 Size: 920 5225 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PmlI CAC'GTG Cuts at: 2709 2826 2709 Size: 117 6028 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2543 2575 4214 4246 5727 5759 2543 Size: 32 1639 32 1481 32 2929 Fragments arranged by size: 2929 1639 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2049 2049 Size: 6145 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 3715 3715 Size: 6145 PspOMI G'GGCC_C Cuts at: 2082 2082 Size: 6145 PstI C_TGCA'G Cuts at: 830 2485 2882 2935 3497 830 Size: 1655 397 53 562 3478 Fragments arranged by size: 3478 1655 562 397 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 2416 2416 Size: 6145 SacII CC_GC'GG Cuts at: 2057 2848 2057 Size: 791 5354 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SanDI GG'GwC_CC Cuts at: 2049 2049 Size: 6145 SapI GCTCTTCn'nnn_ Cuts at: 3081 3081 Size: 6145 SbfI CC_TGCA'GG Cuts at: 2935 3497 2935 Size: 562 5583 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SexAI A'CCwGG_T Cuts at: 2197 2197 Size: 6145 SfcI C'TryA_G Cuts at: 826 1045 2481 2878 2931 3063 3493 4628 5309 Size: 219 1436 397 53 132 430 1135 681 Cuts at: 5309 5500 826 Size: 191 1471 Fragments arranged by size: 1471 1436 1135 681 430 397 219 191 132 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 3472 1872 Size: 1600 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3425 4249 5150 5412 820 Size: 197 926 1482 824 901 262 1553 Fragments arranged by size: 1553 1482 926 901 824 262 197 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 3503 1132 Size: 2371 3774 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6145 StyI C'CwwG_G Cuts at: 513 1065 2004 2397 2748 3354 3584 513 Size: 552 939 393 351 606 230 3074 Fragments arranged by size: 3074 939 606 552 393 351 230 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3158 4172 4189 4342 4527 4972 325 Size: 1255 1578 1014 17 153 185 445 1498 Fragments arranged by size: 1578 1498 1255 1014 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2240 4391 6103 Size: 274 80 33 51 1443 263 2151 1712 Cuts at: 6103 96 Size: 138 Fragments arranged by size: 2151 1712 1443 274 263 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2824 2856 6105 6137 2824 Size: 32 3249 32 2832 Fragments arranged by size: 3249 2832 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 3481 3481 Size: 6145 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2101 2101 Size: 6145 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6145 XmaI C'CCGG_G Cuts at: 1870 3470 1870 Size: 1600 4545 XmnI GAAnn'nnTTC Cuts at: 4274 4274 Size: 6145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3953 276 Size: 53 83 186 936 2419 2468 Fragments arranged by size: 2468 2419 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SfiI SfoI SrfI SwaI