Restriction map for
pFN21AE0462
(Circular) (Six-base) MAPSORT of: pFN21AE0462.seq Check: 2462 from: 1 to: 5386
pFN21AE0462 5386 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2000 2000
Size: 5386
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3196 278
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2729 1937
Size: 792 4594
Acc65I G'GTAC_C
Cuts at: 2707 2707
Size: 5386
AclI AA'CG_TT
Cuts at: 1563 3513 3886 1563
Size: 1950 373 3063
Fragments arranged by size:
3063 1950 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3449 4833 5101 1632
Size: 1817 1384 268 1917
Fragments arranged by size:
1917 1817 1384 268
AfeI AGC'GCT
Cuts at: 2123 2552 2123
Size: 429 4957
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5189
AflIII A'CryG_T
Cuts at: 1203 4285 5262 1203
Size: 3082 977 1327
Fragments arranged by size:
3082 1327 977
AgeI A'CCGG_T
Cuts at: 4217 4217
Size: 5386
AhdI GACnn_n'nnGTC
Cuts at: 4115 4115
Size: 5386
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5386
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2285 1184
Size: 1101 4285
AlwNI CAG_nnn'CTG
Cuts at: 2053 3129 4701 2053
Size: 1076 1572 2738
Fragments arranged by size:
2738 1572 1076
ApaLI G'TGCA_C
Cuts at: 3443 4599 3443
Size: 1156 4230
ApoI r'AATT_y
Cuts at: 1493 2695 2921 3073 5182 1493
Size: 1202 226 152 2109 1697
Fragments arranged by size:
2109 1697 1202 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3940 160
Size: 872 2908 1606
Fragments arranged by size:
2908 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
AvaI C'yCGr_G
Cuts at: 1870 1943 2711 1870
Size: 73 768 4545
Fragments arranged by size:
4545 768 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2158 2191 2158
Size: 33 5353
BamHI G'GATC_C
Cuts at: 2716 3083 2716
Size: 367 5019
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2707 4162 618
Size: 325 217 627 294 626 1455 1842
Fragments arranged by size:
1842 1455 627 626 325 294 217
BanII G_rGCy'C
Cuts at: 1391 2094 2705 1391
Size: 703 611 4072
Fragments arranged by size:
4072 703 611
BbeI G_GCGC'C
Cuts at: 2085 2085
Size: 5386
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2197 928
Size: 971 298 4117
Fragments arranged by size:
4117 971 298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3577 3611 1903
Size: 34 1640 34 3678
Fragments arranged by size:
3678 1640 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3278 4488 3278
Size: 1210 4176
BclI T'GATC_A
Cuts at: 1548 2497 1548
Size: 949 4437
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3997 136
Size: 107 122 71 631 605 2325 1525
Fragments arranged by size:
2325 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5381 5381
Size: 5386
BlpI GC'TnA_GC
Cuts at: 2803 2803
Size: 5386
Bme1580I G_kGCm'C
Cuts at: 1790 3447 4603 1790
Size: 1657 1156 2573
Fragments arranged by size:
2573 1657 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5386
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3117 4075 442
Size: 416 53 83 399 24 1700 958 1753
Fragments arranged by size:
1753 1700 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4046 1239
Size: 2807 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2639 3511 4376 4674 2639
Size: 872 865 298 3351
Fragments arranged by size:
3351 872 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2105 2144 4049 882
Size: 617 606 39 1905 2219
Fragments arranged by size:
2219 1905 617 606 39
BsaAI yAC'GTr
Cuts at: 493 1204 2239 3177 493
Size: 711 1035 938 2702
Fragments arranged by size:
2702 1035 938 711
BsaBI GATnn'nnATC
Cuts at: 5380 5380
Size: 5386
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 3193 3575 4210
Size: 53 83 186 936 549 1111 382 635
Cuts at: 4210 275
Size: 1451
Fragments arranged by size:
1451 1111 936 635 549 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3819 4217 4491 4638 5049 3819
Size: 398 274 147 411 4156
Fragments arranged by size:
4156 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5161
Fragments arranged by size:
5161 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5386
BseYI C'CCAG_C
Cuts at: 2021 4589 5085 2021
Size: 2568 496 2322
Fragments arranged by size:
2568 2322 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2330 1163
Size: 573 594 4219
Fragments arranged by size:
4219 594 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2836 3597 4625 1540
Size: 275 186 835 761 1028 2301
Fragments arranged by size:
2301 1028 835 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2576 3447 3532 4603 2576
Size: 871 85 1071 3359
Fragments arranged by size:
3359 1071 871 85
BsmI GAATG_Cn'
Cuts at: 2897 2990 2897
Size: 93 5293
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2576 2705 3447 3532 4603 1391
Size: 399 304 482 129 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 482 399 304 129 85
BspEI T'CCGG_A
Cuts at: 5049 5049
Size: 5386
BspHI T'CATG_A
Cuts at: 3275 5005 3275
Size: 1730 3656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2000 2741 844
Size: 1156 741 3489
Fragments arranged by size:
3489 1156 741
BsrBI CCG'CTC
Cuts at: 1394 1823 3273 1394
Size: 429 1450 3507
Fragments arranged by size:
3507 1450 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2601 3881 4055 65
Size: 1183 1353 1280 174 1396
Fragments arranged by size:
1396 1353 1280 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4030 4217 1700
Size: 110 2220 187 2869
Fragments arranged by size:
2869 2220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5386
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5386
BssSI C'ACGA_G
Cuts at: 3446 4458 3446
Size: 1012 4374
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2053 794
Size: 1259 4127
BstBI TT'CG_AA
Cuts at: 4190 4190
Size: 5386
BstEII G'GTnAC_C
Cuts at: 1184 2327 1184
Size: 1143 4243
BstXI CCAn_nnnn'nTGG
Cuts at: 2680 2680
Size: 5386
BstYI r'GATC_y
Cuts at: 1972 2716 3083 3468 3485 4937 5023 5035 5381
Size: 744 367 385 17 1452 86 12 346
Cuts at: 5381 1972
Size: 1977
Fragments arranged by size:
1977 1452 744 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2154 513
Size: 552 939 150 3745
Fragments arranged by size:
3745 939 552 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2051 487
Size: 683 56 825 3822
Fragments arranged by size:
3822 825 683 56
BtsI GCAGTG_nn'
Cuts at: 2902 3696 3716 2902
Size: 794 20 4572
Fragments arranged by size:
4572 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5351
DraI TTT'AAA
Cuts at: 2690 2690
Size: 5386
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4393 809
Size: 507 3077 1802
Fragments arranged by size:
3077 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2833 3722 5351
Size: 54 1425 50 275 18 1003 889 1629
Cuts at: 5351 8
Size: 43
Fragments arranged by size:
1629 1425 1003 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2833 1537
Size: 275 1021 4090
Fragments arranged by size:
4090 1021 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2378 2541 3316 1353
Size: 1025 163 775 3423
Fragments arranged by size:
3423 1025 775 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3973 4347 4493 387
Size: 1064 138 2384 374 146 1280
Fragments arranged by size:
2384 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3449 4046 4833 5101 1239
Size: 393 1817 597 787 268 1524
Fragments arranged by size:
1817 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2050 2251 2587 2050
Size: 201 336 4849
Fragments arranged by size:
4849 336 201
EcoRI G'AATT_C
Cuts at: 2695 3073 2695
Size: 378 5008
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2221 2680 2221
Size: 459 4927
FspI TGC'GCA
Cuts at: 3892 3892
Size: 5386
HaeII r_GCGC'y
Cuts at: 1678 2085 2125 2554 4533 1678
Size: 407 40 429 1979 2531
Fragments arranged by size:
2531 1979 429 407 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4027 4059 4101 4133 1373
Size: 32 135 32 2455 32 42 32 2626
Fragments arranged by size:
2626 2455 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2730 2976 669
Size: 988 281 792 246 3079
Fragments arranged by size:
3079 988 792 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5386
HpaI GTT'AAC
Cuts at: 2976 2976
Size: 5386
KasI G'GCGC_C
Cuts at: 2081 2081
Size: 5386
KpnI G_GTAC'C
Cuts at: 2711 2711
Size: 5386
MfeI C'AATT_G
Cuts at: 2985 2985
Size: 5386
MluI A'CGCG_T
Cuts at: 5262 5262
Size: 5386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2581 4500 4684 1305
Size: 1276 1919 184 2007
Fragments arranged by size:
2007 1919 1276 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3564
Fragments arranged by size:
3564 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2237 3344 3703 3862 518
Size: 648 1071 1107 359 159 2042
Fragments arranged by size:
2042 1107 1071 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2802 3479 4627 4872 1606
Size: 1196 677 1148 245 2120
Fragments arranged by size:
2120 1196 1148 677 245
NarI GG'CG_CC
Cuts at: 2082 2082
Size: 5386
NcoI C'CATG_G
Cuts at: 513 1065 2004 2154 513
Size: 552 939 150 3745
Fragments arranged by size:
3745 939 552 150
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5386
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5386
NotI GC'GGCC_GC
Cuts at: 2833 2833
Size: 5386
NspI r_CATG'y
Cuts at: 1132 1229 2236 2426 2744 4289 1132
Size: 97 1007 190 318 1545 2229
Fragments arranged by size:
2229 1545 1007 318 190 97
PasI CC'CwG_GG
Cuts at: 1348 2086 1348
Size: 738 4648
PciI A'CATG_T
Cuts at: 4285 4285
Size: 5386
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5386
PmeI GTTT'AAAC
Cuts at: 2690 2690
Size: 5386
PmlI CAC'GTG
Cuts at: 2239 2239
Size: 5386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3455 3487 4968 5000 3455
Size: 32 1481 32 3841
Fragments arranged by size:
3841 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2251 2587 2251
Size: 336 5050
PshAI GACnn'nnGTC
Cuts at: 1613 2156 1613
Size: 543 4843
PsiI TTA'TAA
Cuts at: 2956 2956
Size: 5386
PstI C_TGCA'G
Cuts at: 830 2738 830
Size: 1908 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5386
SalI G'TCGA_C
Cuts at: 1936 2728 1936
Size: 792 4594
SapI GCTCTTCn'nnn_
Cuts at: 2541 2541
Size: 5386
SbfI CC_TGCA'GG
Cuts at: 2738 2738
Size: 5386
ScaI AGT'ACT
Cuts at: 3634 5346 3634
Size: 1712 3674
SfcI C'TryA_G
Cuts at: 826 1045 2471 2734 3869 4550 4741 826
Size: 219 1426 263 1135 681 191 1471
Fragments arranged by size:
1471 1426 1135 681 263 219 191
SfoI GGC'GCC
Cuts at: 2083 2083
Size: 5386
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5386
SmaI CCC'GGG
Cuts at: 1872 2713 1872
Size: 841 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2654 3490 4391 4653 820
Size: 197 926 711 836 901 262 1553
Fragments arranged by size:
1553 926 901 836 711 262 197
SnaBI TAC'GTA
Cuts at: 493 3177 493
Size: 2684 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5386
SphI G_CATG'C
Cuts at: 1132 2236 2426 2744 1132
Size: 1104 190 318 3774
Fragments arranged by size:
3774 1104 318 190
SspI AAT'ATT
Cuts at: 5 52 3310 5
Size: 47 3258 2081
Fragments arranged by size:
3258 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5386
StyI C'CwwG_G
Cuts at: 513 1065 2004 2154 2825 513
Size: 552 939 150 671 3074
Fragments arranged by size:
3074 939 671 552 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2129 3413 3430 3583 3768 4213 325
Size: 1255 549 1284 17 153 185 445 1498
Fragments arranged by size:
1498 1284 1255 549 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3632 5344 96
Size: 274 80 33 51 1443 1655 1712 138
Fragments arranged by size:
1712 1655 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5346 5378 5346
Size: 32 5354
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5386
XbaI T'CTAG_A
Cuts at: 2722 2722
Size: 5386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2680 2680
Size: 5386
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5386
XmaI C'CCGG_G
Cuts at: 1870 2711 1870
Size: 841 4545
XmnI GAAnn'nnTTC
Cuts at: 2511 3515 2511
Size: 1004 4382
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3194 276
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI