(Circular) (Six-base) MAPSORT of: pFN21AE0429.seq Check: 2729 from: 1 to: 5989 pFN21AE0429 5989 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3220 3220 Size: 5989 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3799 278 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3332 1937 Size: 1395 4594 Acc65I G'GTAC_C Cuts at: 2670 3310 2670 Size: 640 5349 AclI AA'CG_TT Cuts at: 1563 4116 4489 1563 Size: 2553 373 3063 Fragments arranged by size: 3063 2553 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2484 4052 5436 5704 1632 Size: 852 1568 1384 268 1917 Fragments arranged by size: 1917 1568 1384 852 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5792 AflIII A'CryG_T Cuts at: 1203 4888 5865 1203 Size: 3685 977 1327 Fragments arranged by size: 3685 1327 977 AgeI A'CCGG_T Cuts at: 4820 4820 Size: 5989 AhdI GACnn_n'nnGTC Cuts at: 4718 4718 Size: 5989 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5989 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5989 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3239 3271 3239 Size: 32 5957 AlwNI CAG_nnn'CTG Cuts at: 3732 5304 3732 Size: 1572 4417 ApaI G_GGCC'C Cuts at: 2024 2107 2268 2024 Size: 83 161 5745 Fragments arranged by size: 5745 161 83 ApaLI G'TGCA_C Cuts at: 2733 4046 5202 2733 Size: 1313 1156 3520 Fragments arranged by size: 3520 1313 1156 ApoI r'AATT_y Cuts at: 1493 3298 3524 3676 5785 1493 Size: 1805 226 152 2109 1697 Fragments arranged by size: 2109 1805 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4543 160 Size: 872 3511 1606 Fragments arranged by size: 3511 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 AvaI C'yCGr_G Cuts at: 1870 1943 2189 2261 2267 3314 1870 Size: 73 246 72 6 1047 4545 Fragments arranged by size: 4545 1047 246 73 72 6 BamHI G'GATC_C Cuts at: 3319 3686 3319 Size: 367 5622 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2670 3232 3310 4765 618 Size: 325 217 627 883 562 78 1455 1842 Fragments arranged by size: 1842 1455 883 627 562 325 217 78 BanII G_rGCy'C Cuts at: 1391 2024 2107 2268 2348 3308 1391 Size: 633 83 161 80 960 4072 Fragments arranged by size: 4072 960 633 161 83 80 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2694 928 Size: 971 795 4223 Fragments arranged by size: 4223 971 795 BbvCI CC'TCA_GC Cuts at: 3213 3213 Size: 5989 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4180 4214 1903 Size: 34 2243 34 3678 Fragments arranged by size: 3678 2243 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3881 5091 3881 Size: 1210 4779 BclI T'GATC_A Cuts at: 1548 2173 1548 Size: 625 5364 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2626 4600 136 Size: 107 122 71 631 605 954 1974 1525 Fragments arranged by size: 1974 1525 954 631 605 122 107 71 BglII A'GATC_T Cuts at: 5984 5984 Size: 5989 BlpI GC'TnA_GC Cuts at: 3406 3406 Size: 5989 Bme1580I G_kGCm'C Cuts at: 1790 2024 2107 2262 2268 2737 3237 3284 4050 Size: 234 83 155 6 469 500 47 766 Cuts at: 4050 5206 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 766 500 469 234 155 83 47 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5989 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2506 2589 2841 Size: 416 53 83 399 24 1089 83 252 Cuts at: 2841 3720 4678 442 Size: 879 958 1753 Fragments arranged by size: 1753 1089 958 879 416 399 252 83 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5989 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2356 3200 4649 1239 Size: 1117 844 1449 2579 Fragments arranged by size: 2579 1449 1117 844 Bpu10I CC'TnA_GC Cuts at: 2133 2908 3213 2133 Size: 775 305 4909 Fragments arranged by size: 4909 775 305 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2093 4114 4979 5277 2093 Size: 2021 865 298 2805 Fragments arranged by size: 2805 2021 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4652 882 Size: 617 3153 2219 Fragments arranged by size: 3153 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3780 493 Size: 711 2576 2702 Fragments arranged by size: 2702 2576 711 BsaBI GATnn'nnATC Cuts at: 5983 5983 Size: 5989 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3796 4178 4813 275 Size: 53 83 186 936 2263 382 635 1451 Fragments arranged by size: 2263 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4422 4820 5094 5241 5652 4422 Size: 398 274 147 411 4759 Fragments arranged by size: 4759 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3100 3130 1178 Size: 30 165 30 1697 30 4037 Fragments arranged by size: 4037 1697 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2431 2560 2974 2977 1186 Size: 1245 129 414 3 4198 Fragments arranged by size: 4198 1245 414 129 3 BseYI C'CCAG_C Cuts at: 2665 3064 5192 5688 2665 Size: 399 2128 496 2966 Fragments arranged by size: 2966 2128 496 399 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2335 1163 Size: 573 599 4817 Fragments arranged by size: 4817 599 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3439 4200 5228 1540 Size: 275 186 1438 761 1028 2301 Fragments arranged by size: 2301 1438 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2348 2737 4050 4135 5206 2348 Size: 389 1313 85 1071 3131 Fragments arranged by size: 3131 1313 1071 389 85 BsmI GAATG_Cn' Cuts at: 3500 3593 3500 Size: 93 5896 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2107 2262 2268 2348 2737 3237 Size: 399 234 83 155 6 80 389 500 Cuts at: 3237 3284 3308 4050 4135 5206 1391 Size: 47 24 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 500 399 389 234 155 85 83 80 47 24 6 BspEI T'CCGG_A Cuts at: 5652 5652 Size: 5989 BspHI T'CATG_A Cuts at: 3878 5608 3878 Size: 1730 4259 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2731 3220 3344 844 Size: 1887 489 124 3489 Fragments arranged by size: 3489 1887 489 124 BsrBI CCG'CTC Cuts at: 1394 1823 2118 3147 3876 1394 Size: 429 295 1029 729 3507 Fragments arranged by size: 3507 1029 729 429 295 BsrDI GCAATG_nn' Cuts at: 65 1248 4484 4658 65 Size: 1183 3236 174 1396 Fragments arranged by size: 3236 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4633 4820 1700 Size: 110 2823 187 2869 Fragments arranged by size: 2869 2823 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5989 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5989 BssSI C'ACGA_G Cuts at: 3277 4049 5061 3277 Size: 772 1012 4205 Fragments arranged by size: 4205 1012 772 BstAPI GCAn_nnn'nTGC Cuts at: 794 2213 2918 3232 794 Size: 1419 705 314 3551 Fragments arranged by size: 3551 1419 705 314 BstBI TT'CG_AA Cuts at: 4793 4793 Size: 5989 BstEII G'GTnAC_C Cuts at: 1184 2194 3037 1184 Size: 1010 843 4136 Fragments arranged by size: 4136 1010 843 BstYI r'GATC_y Cuts at: 1972 2992 3254 3319 3686 4071 4088 5540 5626 Size: 1020 262 65 367 385 17 1452 86 Cuts at: 5626 5638 5984 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1020 385 367 346 262 86 65 17 12 Bsu36I CC'TnA_GG Cuts at: 2487 2558 2487 Size: 71 5918 BtgI C'CryG_G Cuts at: 513 1065 2004 2388 513 Size: 552 939 384 4114 Fragments arranged by size: 4114 939 552 384 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5250 Fragments arranged by size: 5250 683 56 BtsI GCAGTG_nn' Cuts at: 2437 2735 3505 4299 4319 2437 Size: 298 770 794 20 4107 Fragments arranged by size: 4107 794 770 298 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5989 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5954 DraI TTT'AAA Cuts at: 3293 3293 Size: 5989 DraIII CAC_nnn'GTG Cuts at: 2514 2514 Size: 5989 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2153 2875 4996 809 Size: 507 837 722 2121 1802 Fragments arranged by size: 2121 1802 837 722 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3436 4325 5954 Size: 54 1425 50 275 18 1606 889 1629 Cuts at: 5954 8 Size: 43 Fragments arranged by size: 1629 1606 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3436 1537 Size: 275 1624 4090 Fragments arranged by size: 4090 1624 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2503 3016 3919 1353 Size: 1150 513 903 3423 Fragments arranged by size: 3423 1150 903 513 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4576 4950 5096 387 Size: 1064 138 2987 374 146 1280 Fragments arranged by size: 2987 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2346 2346 Size: 5989 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2356 2484 3200 4052 4649 5436 5704 Size: 393 724 128 716 852 597 787 268 Cuts at: 5704 1239 Size: 1524 Fragments arranged by size: 1524 852 787 724 716 597 393 268 128 EcoO109I rG'GnC_Cy Cuts at: 2020 2103 2104 2146 2185 2926 2972 3193 2020 Size: 83 1 42 39 741 46 221 4816 Fragments arranged by size: 4816 741 221 83 46 42 39 1 EcoRI G'AATT_C Cuts at: 3298 3676 3298 Size: 378 5611 FspI TGC'GCA Cuts at: 4495 4495 Size: 5989 HaeII r_GCGC'y Cuts at: 1678 5136 1678 Size: 3458 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4630 4662 4704 4736 1373 Size: 32 135 32 3058 32 42 32 2626 Fragments arranged by size: 3058 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3333 3579 669 Size: 988 281 1395 246 3079 Fragments arranged by size: 3079 1395 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5989 HpaI GTT'AAC Cuts at: 3579 3579 Size: 5989 KpnI G_GTAC'C Cuts at: 2674 3314 2674 Size: 640 5349 MfeI C'AATT_G Cuts at: 3588 3588 Size: 5989 MluI A'CGCG_T Cuts at: 5865 5865 Size: 5989 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5103 5287 1305 Size: 3798 184 2007 Fragments arranged by size: 3798 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4167 Fragments arranged by size: 4167 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3947 4306 4465 518 Size: 648 2781 359 159 2042 Fragments arranged by size: 2781 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2446 3068 3125 3223 3405 4082 5230 5475 Size: 840 622 57 98 182 677 1148 245 Cuts at: 5475 1606 Size: 2120 Fragments arranged by size: 2120 1148 840 677 622 245 182 98 57 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4498 Fragments arranged by size: 4498 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5989 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5989 NotI GC'GGCC_GC Cuts at: 3436 3436 Size: 5989 NspI r_CATG'y Cuts at: 1132 1229 3347 4892 1132 Size: 97 2118 1545 2229 Fragments arranged by size: 2229 2118 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5989 PciI A'CATG_T Cuts at: 4888 4888 Size: 5989 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5989 PfoI T'CCnGG_A Cuts at: 2336 3266 2336 Size: 930 5059 PmeI GTTT'AAAC Cuts at: 3293 3293 Size: 5989 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4058 4090 5571 5603 4058 Size: 32 1481 32 4444 Fragments arranged by size: 4444 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2146 2185 2926 2146 Size: 39 741 5209 Fragments arranged by size: 5209 741 39 PshAI GACnn'nnGTC Cuts at: 1613 2079 1613 Size: 466 5523 PsiI TTA'TAA Cuts at: 3559 3559 Size: 5989 PspOMI G'GGCC_C Cuts at: 2020 2103 2264 2020 Size: 83 161 5745 Fragments arranged by size: 5745 161 83 PstI C_TGCA'G Cuts at: 830 2280 2742 2771 2923 3124 3228 3341 830 Size: 1450 462 29 152 201 104 113 3478 Fragments arranged by size: 3478 1450 462 201 152 113 104 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5989 PvuII CAG'CTG Cuts at: 2446 3068 3125 3223 2446 Size: 622 57 98 5212 Fragments arranged by size: 5212 622 98 57 SacI G_AGCT'C Cuts at: 2348 2348 Size: 5989 SalI G'TCGA_C Cuts at: 1936 3331 1936 Size: 1395 4594 SanDI GG'GwC_CC Cuts at: 2185 2926 2185 Size: 741 5248 SbfI CC_TGCA'GG Cuts at: 3341 3341 Size: 5989 ScaI AGT'ACT Cuts at: 4237 5949 4237 Size: 1712 4277 SfcI C'TryA_G Cuts at: 826 1045 2276 2380 2738 2767 2919 2934 3120 Size: 219 1231 104 358 29 152 15 186 Cuts at: 3120 3224 3337 4472 5153 5344 826 Size: 104 113 1135 681 191 1471 Fragments arranged by size: 1471 1231 1135 681 358 219 191 186 152 113 104 104 29 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5989 SmaI CCC'GGG Cuts at: 1872 2263 2269 3316 1872 Size: 391 6 1047 4545 Fragments arranged by size: 4545 1047 391 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 2108 4093 4994 5256 820 Size: 197 926 165 1985 901 262 1553 Fragments arranged by size: 1985 1553 926 901 262 197 165 SnaBI TAC'GTA Cuts at: 493 3780 493 Size: 3287 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5989 SphI G_CATG'C Cuts at: 1132 3347 1132 Size: 2215 3774 SrfI GCCC'GGGC Cuts at: 2263 2269 2263 Size: 6 5983 SspI AAT'ATT Cuts at: 5 52 3913 5 Size: 47 3861 2081 Fragments arranged by size: 3861 2081 47 StuI AGG'CCT Cuts at: 1517 2244 2485 2585 1517 Size: 727 241 100 4921 Fragments arranged by size: 4921 727 241 100 StyI C'CwwG_G Cuts at: 513 1065 2004 3428 513 Size: 552 939 1424 3074 Fragments arranged by size: 3074 1424 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2525 2860 4016 4033 4186 4371 4816 Size: 1255 945 335 1156 17 153 185 445 Cuts at: 4816 325 Size: 1498 Fragments arranged by size: 1498 1255 1156 945 445 335 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2597 4235 5947 Size: 274 80 33 51 1443 620 1638 1712 Cuts at: 5947 96 Size: 138 Fragments arranged by size: 1712 1638 1443 620 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2780 2812 3100 3132 5949 5981 2000 Size: 32 748 32 288 32 2817 32 2008 Fragments arranged by size: 2817 2008 748 288 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5989 XbaI T'CTAG_A Cuts at: 3325 3325 Size: 5989 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2165 2165 Size: 5989 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5989 XmaI C'CCGG_G Cuts at: 1870 2261 2267 3314 1870 Size: 391 6 1047 4545 Fragments arranged by size: 4545 1047 391 6 XmnI GAAnn'nnTTC Cuts at: 4118 4118 Size: 5989 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3797 276 Size: 53 83 186 936 2263 2468 Fragments arranged by size: 2468 2263 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SwaI