Restriction map for
pFN21AB8679
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI AscI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BbvCI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AfaI 365 GTAC GT^AC
AflIII 143 ACACGT A^C[GA][CT]GT
Alw44I 691 GTGCAC G^TGCAC
AlwNI 808 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 512 GGGCCC GGGCC^C
ApaLI 691 GTGCAC G^TGCAC
Asp700I 100 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 691 GTGCAC G[AT]GC[AT]^C
BamHI 372 GGATCC G^GATCC
BanII 512 GGGCCC G[GA]GC[CT]^C
BbsI 348 GAAGAC GAAGAC(2/6)
BcgI 729 GCAGGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 332 GGATAC GTATCC(6/5)
BpuAI 348 GAAGAC GAAGAC(2/6)
BsiHKAI 691 GTGCAC G[AT]GC[AT]^C
BsmBI 146 CGTCTC CGTCTC(1/5)
BspHI 758 TCATGA T^CATGA
BsrDI 62 CATTGC GCAATG(2/0)
BsrI 385 ACTGG ACTGG(1/-1)
BstXI 416 CCACTCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 372 GGATCC [GA]^GATC[CT]
DraIII 775 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1077 CCATGG C^C[GA][CT]GG
EcoT22I 787 ATGCAT ATGCA^T
HapII 471 CCGG C^CGG
HpaII 471 CCGG C^CGG
MaeII 145 ACGT A^CGT
MflI 372 GGATCC [GA]^GATC[CT]
MslI 826 CACAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1055 CAGCGG C[AC]G^C[GT]G
MspI 471 CCGG C^CGG
NcoI 1077 CCATGG C^CATGG
NsiI 787 ATGCAT ATGCA^T
NspI 217 GCATGT [GA]CATG^[CT]
PflMI 306 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 496 GGGACCC [GA]G^G[AT]CC[CT]
PstI 563 CTGCAG CTGCA^G
RcaI 758 TCATGA T^CATGA
RsaI 365 GTAC GT^AC
Van91I 306 CCATGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 372 GGATCC [GA]^GATC[CT]
XmnI 100 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 103 GAATTT [GA]^AATT[CT]
171 GAATTT
ApoI 103 GAATTT [GA]^AATT[CT]
171 GAATTT
AseI 274 ATTAAT AT^TAAT
1044 ATTAAT
AsnI 274 ATTAAT AT^TAAT
1044 ATTAAT
BanI 532 GGTGCC G^G[CT][GA]CC
667 GGCACC
BfaI 509 CTAG C^TAG
517 CTAG
BseRI 1373 CTCCTC GAGGAG(10/8)
1385 CTCCTC
BsrBI 52 GAGCGG CCGCTC(-3/-3)
1452 GAGCGG
BstF5I 6 GGATG GGATG(2/0)
831 GGATG
Cac8I 445 GCAGGC GC[ACGT]^[ACGT]GC
664 GCTGGC
FauI 1302 CCCGC CCCGC(4/6)
1454 GCGGG
FokI 6 GGATG GGATG(9/13)
831 GGATG
HincII 876 GTTGAC GT[CT]^[GA]AC
1249 GTTGAC
HindII 876 GTTGAC GT[CT]^[GA]AC
1249 GTTGAC
MaeI 509 CTAG C^TAG
517 CTAG
PshBI 274 ATTAAT AT^TAAT
1044 ATTAAT
SfcI 563 CTGCAG C^T[GA][CT]AG
1254 CTACAG
TaqI 327 TCGA T^CGA
963 TCGA
TthHB8I 327 TCGA T^CGA
963 TCGA
XcmI 1268 CCAAAAAAGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1436 CCAAAGCTGGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BclI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BssKI BstAPI BstNI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI HaeIII
HinfI HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI