(Circular) (Six-base) MAPSORT of: pFN21AB8679.seq Check: 1761 from: 1 to: 6247 pFN21AB8679 6247 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3238 3238 Size: 6247 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4057 278 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3590 1937 Size: 1653 4594 Acc65I G'GTAC_C Cuts at: 3568 3568 Size: 6247 AclI AA'CG_TT Cuts at: 1563 4374 4747 1563 Size: 2811 373 3063 Fragments arranged by size: 3063 2811 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2124 2261 3312 4310 5694 5962 1632 Size: 492 137 1051 998 1384 268 1917 Fragments arranged by size: 1917 1384 1051 998 492 268 137 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6050 AflIII A'CryG_T Cuts at: 1203 2148 5146 6123 1203 Size: 945 2998 977 1327 Fragments arranged by size: 2998 1327 977 945 AgeI A'CCGG_T Cuts at: 5078 5078 Size: 6247 AhdI GACnn_n'nnGTC Cuts at: 4976 4976 Size: 6247 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6247 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3333 1184 Size: 2149 4098 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2737 2769 2737 Size: 32 6215 AlwNI CAG_nnn'CTG Cuts at: 2818 3990 5562 2818 Size: 1172 1572 3503 Fragments arranged by size: 3503 1572 1172 ApaI G_GGCC'C Cuts at: 2521 2521 Size: 6247 ApaLI G'TGCA_C Cuts at: 2696 4304 5460 2696 Size: 1608 1156 3483 Fragments arranged by size: 3483 1608 1156 ApoI r'AATT_y Cuts at: 1493 2108 2176 3556 3782 3934 6043 1493 Size: 615 68 1380 226 152 2109 1697 Fragments arranged by size: 2109 1697 1380 615 226 152 68 AseI AT'TA_AT Cuts at: 160 1032 2280 3050 4801 160 Size: 872 1248 770 1751 1606 Fragments arranged by size: 1751 1606 1248 872 770 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 AvaI C'yCGr_G Cuts at: 1870 1943 3572 1870 Size: 73 1629 4545 Fragments arranged by size: 4545 1629 73 BamHI G'GATC_C Cuts at: 2377 3577 3944 2377 Size: 1200 367 4680 Fragments arranged by size: 4680 1200 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2537 2672 3568 5023 618 Size: 325 217 627 750 135 896 1455 1842 Fragments arranged by size: 1842 1455 896 750 627 325 217 135 BanII G_rGCy'C Cuts at: 1391 2521 3566 1391 Size: 1130 1045 4072 Fragments arranged by size: 4072 1130 1045 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2360 928 Size: 971 461 4815 Fragments arranged by size: 4815 971 461 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2723 2757 4438 4472 1903 Size: 34 786 34 1681 34 3678 Fragments arranged by size: 3678 1681 786 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2331 4139 5349 2331 Size: 1808 1210 3229 Fragments arranged by size: 3229 1808 1210 BclI T'GATC_A Cuts at: 1548 3111 3180 3399 1548 Size: 1563 69 219 4396 Fragments arranged by size: 4396 1563 219 69 BfrBI ATG'CAT Cuts at: 2794 2794 Size: 6247 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4858 136 Size: 107 122 71 631 605 3186 1525 Fragments arranged by size: 3186 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6242 6242 Size: 6247 BlpI GC'TnA_GC Cuts at: 3664 3664 Size: 6247 Bme1580I G_kGCm'C Cuts at: 1790 2364 2521 2700 4308 5464 1790 Size: 574 157 179 1608 1156 2573 Fragments arranged by size: 2573 1608 1156 574 179 157 BmgBI CAC'GTC Cuts at: 1318 2151 1318 Size: 833 5414 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3978 4936 442 Size: 416 53 83 399 24 2561 958 1753 Fragments arranged by size: 2561 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6247 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2778 3366 3473 3492 4907 1239 Size: 1539 588 107 19 1415 2579 Fragments arranged by size: 2579 1539 1415 588 107 19 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4372 5237 5535 4372 Size: 865 298 5084 Fragments arranged by size: 5084 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4910 882 Size: 617 3411 2219 Fragments arranged by size: 3411 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4038 493 Size: 711 2834 2702 Fragments arranged by size: 2834 2702 711 BsaBI GATnn'nnATC Cuts at: 6241 6241 Size: 6247 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4054 4436 5071 275 Size: 53 83 186 936 2521 382 635 1451 Fragments arranged by size: 2521 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4680 5078 5352 5499 5910 4680 Size: 398 274 147 411 5017 Fragments arranged by size: 5017 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2048 2078 2737 2767 1178 Size: 30 165 30 645 30 659 30 4658 Fragments arranged by size: 4658 659 645 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3369 3381 1186 Size: 2183 12 4052 Fragments arranged by size: 4052 2183 12 BseYI C'CCAG_C Cuts at: 2505 5450 5946 2505 Size: 2945 496 2806 Fragments arranged by size: 2945 2806 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2465 2469 2937 1163 Size: 573 729 4 468 4473 Fragments arranged by size: 4473 729 573 468 4 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3697 4458 5486 1540 Size: 275 186 1696 761 1028 2301 Fragments arranged by size: 2301 1696 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2700 4308 4393 5464 2700 Size: 1608 85 1071 3483 Fragments arranged by size: 3483 1608 1071 85 BsmI GAATG_Cn' Cuts at: 3758 3851 3758 Size: 93 6154 BsmBI CGTCTCn'nnnn_ Cuts at: 2157 2157 Size: 6247 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2364 2521 2700 3566 4308 4393 5464 Size: 399 574 157 179 866 742 85 1071 Cuts at: 5464 1391 Size: 2174 Fragments arranged by size: 2174 1071 866 742 574 399 179 157 85 BspEI T'CCGG_A Cuts at: 5910 5910 Size: 6247 BspHI T'CATG_A Cuts at: 2763 4136 5866 2763 Size: 1373 1730 3144 Fragments arranged by size: 3144 1730 1373 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2856 3083 3238 3301 3602 844 Size: 2012 227 155 63 301 3489 Fragments arranged by size: 3489 2012 301 227 155 63 BsrBI CCG'CTC Cuts at: 1394 1823 2059 3459 4134 1394 Size: 429 236 1400 675 3507 Fragments arranged by size: 3507 1400 675 429 236 BsrDI GCAATG_nn' Cuts at: 65 1248 2066 4742 4916 65 Size: 1183 818 2676 174 1396 Fragments arranged by size: 2676 1396 1183 818 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4891 5078 1700 Size: 110 3081 187 2869 Fragments arranged by size: 3081 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6247 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6247 BssSI C'ACGA_G Cuts at: 4307 5319 4307 Size: 1012 5235 BstAPI GCAn_nnn'nTGC Cuts at: 794 2800 2924 3335 794 Size: 2006 124 411 3706 Fragments arranged by size: 3706 2006 411 124 BstBI TT'CG_AA Cuts at: 5051 5051 Size: 6247 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6247 BstXI CCAn_nnnn'nTGG Cuts at: 2428 2428 Size: 6247 BstYI r'GATC_y Cuts at: 1972 2377 3577 3944 4329 4346 5798 5884 5896 Size: 405 1200 367 385 17 1452 86 12 Cuts at: 5896 6242 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1200 405 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3082 513 Size: 552 939 1078 3678 Fragments arranged by size: 3678 1078 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5508 Fragments arranged by size: 5508 683 56 BtsI GCAGTG_nn' Cuts at: 3763 4557 4577 3763 Size: 794 20 5433 Fragments arranged by size: 5433 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6247 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6212 DraI TTT'AAA Cuts at: 3551 3551 Size: 6247 DraIII CAC_nnn'GTG Cuts at: 2785 2785 Size: 6247 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5254 809 Size: 507 3938 1802 Fragments arranged by size: 3938 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3694 4583 6212 Size: 54 1425 50 275 18 1864 889 1629 Cuts at: 6212 8 Size: 43 Fragments arranged by size: 1864 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3694 1537 Size: 275 1882 4090 Fragments arranged by size: 4090 1882 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2237 2311 3413 4177 1353 Size: 884 74 1102 764 3423 Fragments arranged by size: 3423 1102 884 764 74 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4834 5208 5354 387 Size: 1064 138 435 2810 374 146 1280 Fragments arranged by size: 2810 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2124 2261 2778 3312 3366 3473 3492 Size: 393 492 137 517 534 54 107 19 Cuts at: 3492 4310 4907 5694 5962 1239 Size: 818 597 787 268 1524 Fragments arranged by size: 1524 818 787 597 534 517 492 393 268 137 107 54 19 EcoO109I rG'GnC_Cy Cuts at: 2502 2517 2518 3190 2502 Size: 15 1 672 5559 Fragments arranged by size: 5559 672 15 1 EcoRI G'AATT_C Cuts at: 3556 3934 3556 Size: 378 5869 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2129 2129 Size: 6247 FspI TGC'GCA Cuts at: 4753 4753 Size: 6247 HaeII r_GCGC'y Cuts at: 1678 5394 1678 Size: 3716 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2279 2311 2631 Size: 32 135 32 476 32 199 32 320 Cuts at: 2631 2663 3015 3047 4888 4920 4962 4994 1373 Size: 32 352 32 1841 32 42 32 2626 Fragments arranged by size: 2626 1841 476 352 320 199 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2883 3256 3591 3837 669 Size: 988 281 945 373 335 246 3079 Fragments arranged by size: 3079 988 945 373 335 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6247 HpaI GTT'AAC Cuts at: 3837 3837 Size: 6247 KpnI G_GTAC'C Cuts at: 3572 3572 Size: 6247 MfeI C'AATT_G Cuts at: 3846 3846 Size: 6247 MluI A'CGCG_T Cuts at: 6123 6123 Size: 6247 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2124 5361 5545 1305 Size: 819 3237 184 2007 Fragments arranged by size: 3237 2007 819 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4425 Fragments arranged by size: 4425 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2835 4205 4564 4723 518 Size: 648 1669 1370 359 159 2042 Fragments arranged by size: 2042 1669 1370 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3062 3663 4340 5488 5733 1606 Size: 1456 601 677 1148 245 2120 Fragments arranged by size: 2120 1456 1148 677 601 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3082 513 Size: 552 939 1078 3678 Fragments arranged by size: 3678 1078 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6247 NotI GC'GGCC_GC Cuts at: 3694 3694 Size: 6247 NsiI A_TGCA'T Cuts at: 2796 2796 Size: 6247 NspI r_CATG'y Cuts at: 1132 1229 2226 3605 5150 1132 Size: 97 997 1379 1545 2229 Fragments arranged by size: 2229 1545 1379 997 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6247 PciI A'CATG_T Cuts at: 5146 5146 Size: 6247 PflMI CCAn_nnn'nTGG Cuts at: 1108 2317 1108 Size: 1209 5038 PmeI GTTT'AAAC Cuts at: 3551 3551 Size: 6247 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2737 2769 4316 4348 5829 5861 2737 Size: 32 1547 32 1481 32 3123 Fragments arranged by size: 3123 1547 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2502 2502 Size: 6247 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6247 PsiI TTA'TAA Cuts at: 3817 3817 Size: 6247 PspOMI G'GGCC_C Cuts at: 2517 2517 Size: 6247 PstI C_TGCA'G Cuts at: 830 2572 3599 830 Size: 1742 1027 3478 Fragments arranged by size: 3478 1742 1027 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6247 SalI G'TCGA_C Cuts at: 1936 3589 1936 Size: 1653 4594 SanDI GG'GwC_CC Cuts at: 2502 2502 Size: 6247 SbfI CC_TGCA'GG Cuts at: 3599 3599 Size: 6247 ScaI AGT'ACT Cuts at: 4495 6207 4495 Size: 1712 4535 SfcI C'TryA_G Cuts at: 826 1045 2568 3259 3595 4730 5411 5602 826 Size: 219 1523 691 336 1135 681 191 1471 Fragments arranged by size: 1523 1471 1135 691 681 336 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6247 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6247 SmaI CCC'GGG Cuts at: 1872 3574 1872 Size: 1702 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4351 5252 5514 820 Size: 197 926 2408 901 262 1553 Fragments arranged by size: 2408 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4038 493 Size: 3545 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6247 SphI G_CATG'C Cuts at: 1132 3605 1132 Size: 2473 3774 SspI AAT'ATT Cuts at: 5 52 4171 5 Size: 47 4119 2081 Fragments arranged by size: 4119 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6247 StyI C'CwwG_G Cuts at: 513 1065 2004 2382 2701 3082 3686 513 Size: 552 939 378 319 381 604 3074 Fragments arranged by size: 3074 939 604 552 381 378 319 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2644 4274 4291 4444 4629 5074 325 Size: 1255 1064 1630 17 153 185 445 1498 Fragments arranged by size: 1630 1498 1255 1064 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4493 6205 96 Size: 274 80 33 51 1443 2516 1712 138 Fragments arranged by size: 2516 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2677 2709 6207 6239 2677 Size: 32 3498 32 2685 Fragments arranged by size: 3498 2685 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6247 XbaI T'CTAG_A Cuts at: 3583 3583 Size: 6247 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3280 3448 3280 Size: 168 6079 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6247 XmaI C'CCGG_G Cuts at: 1870 3572 1870 Size: 1702 4545 XmnI GAAnn'nnTTC Cuts at: 2109 4376 2109 Size: 2267 3980 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4055 276 Size: 53 83 186 936 2521 2468 Fragments arranged by size: 2521 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI