Restriction map for
pFN21AB8679
(Linear) (Six-base) MAPSORT of: KIBB8679.seq Check: 6405 from: 1 to: 1542
KIBB8679.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1233 1542
Size: 1233 309
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 256 1307 1542
Size: 119 137 1051 235
Fragments arranged by size:
1051 235 137 119
AflIII A'CryG_T
Cuts at: 0 143 1542
Size: 143 1399
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1328 1542
Size: 1328 214
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 732 764 1542
Size: 732 32 778
Fragments arranged by size:
778 732 32
AlwNI CAG_nnn'CTG
Cuts at: 0 813 1542
Size: 813 729
ApaI G_GGCC'C
Cuts at: 0 516 1542
Size: 516 1026
ApaLI G'TGCA_C
Cuts at: 0 691 1542
Size: 691 851
ApoI r'AATT_y
Cuts at: 0 103 171 1542
Size: 103 68 1371
Fragments arranged by size:
1371 103 68
AseI AT'TA_AT
Cuts at: 0 275 1045 1542
Size: 275 770 497
Fragments arranged by size:
770 497 275
BamHI G'GATC_C
Cuts at: 0 372 1542
Size: 372 1170
BanI G'GyrC_C
Cuts at: 0 532 667 1542
Size: 532 135 875
Fragments arranged by size:
875 532 135
BanII G_rGCy'C
Cuts at: 0 516 1542
Size: 516 1026
BbsI GAAGACnn'nnnn_
Cuts at: 0 355 1542
Size: 355 1187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 718 752 1542
Size: 718 34 790
Fragments arranged by size:
790 718 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 326 1542
Size: 326 1216
BclI T'GATC_A
Cuts at: 0 1106 1175 1394 1542
Size: 1106 69 219 148
Fragments arranged by size:
1106 219 148 69
BfrBI ATG'CAT
Cuts at: 0 789 1542
Size: 789 753
Bme1580I G_kGCm'C
Cuts at: 0 359 516 695 1542
Size: 359 157 179 847
Fragments arranged by size:
847 359 179 157
BmgBI CAC'GTC
Cuts at: 0 146 1542
Size: 146 1396
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 773 1361 1468 1487 1542
Size: 773 588 107 19 55
Fragments arranged by size:
773 588 107 55 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 43 73 732 762 1542
Size: 43 30 659 30 780
Fragments arranged by size:
780 659 43 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1364 1376 1542
Size: 1364 12 166
Fragments arranged by size:
1364 166 12
BseYI C'CCAG_C
Cuts at: 0 500 1542
Size: 500 1042
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 464 932 1542
Size: 460 4 468 610
Fragments arranged by size:
610 468 460 4
BsiHKAI G_wGCw'C
Cuts at: 0 695 1542
Size: 695 847
BsmBI CGTCTCn'nnnn_
Cuts at: 0 152 1542
Size: 152 1390
Bsp1286I G_dGCh'C
Cuts at: 0 359 516 695 1542
Size: 359 157 179 847
Fragments arranged by size:
847 359 179 157
BspHI T'CATG_A
Cuts at: 0 758 1542
Size: 758 784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 851 1078 1233 1296 1542
Size: 851 227 155 63 246
Fragments arranged by size:
851 246 227 155 63
BsrBI CCG'CTC
Cuts at: 0 54 1454 1542
Size: 54 1400 88
Fragments arranged by size:
1400 88 54
BsrDI GCAATG_nn'
Cuts at: 0 61 1542
Size: 61 1481
BstAPI GCAn_nnn'nTGC
Cuts at: 0 795 919 1330 1542
Size: 795 124 411 212
Fragments arranged by size:
795 411 212 124
BstXI CCAn_nnnn'nTGG
Cuts at: 0 423 1542
Size: 423 1119
BstYI r'GATC_y
Cuts at: 0 372 1542
Size: 372 1170
BtgI C'CryG_G
Cuts at: 0 1077 1542
Size: 1077 465
DraIII CAC_nnn'GTG
Cuts at: 0 780 1542
Size: 780 762
EarI CTCTTCn'nnn_
Cuts at: 0 232 306 1408 1542
Size: 232 74 1102 134
Fragments arranged by size:
1102 232 134 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1542
Size: 19 1523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 256 773 1307 1361 1468 1487 1542
Size: 119 137 517 534 54 107 19 55
Fragments arranged by size:
534 517 137 119 107 55 54 19
EcoO109I rG'GnC_Cy
Cuts at: 0 497 512 513 1185 1542
Size: 497 15 1 672 357
Fragments arranged by size:
672 497 357 15 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 124 1542
Size: 124 1418
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 274 306 626 658 1010 1042
Size: 43 32 199 32 320 32 352 32
Cuts at: 1042 1542
Size: 500
Fragments arranged by size:
500 352 320 199 43 32 32 32
32
HincII GTy'rAC
Cuts at: 0 878 1251 1542
Size: 878 373 291
Fragments arranged by size:
878 373 291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 1542
Size: 119 1423
MslI CAynn'nnrTG
Cuts at: 0 830 1542
Size: 830 712
MspA1I CmG'CkG
Cuts at: 0 1057 1542
Size: 1057 485
NcoI C'CATG_G
Cuts at: 0 1077 1542
Size: 1077 465
NsiI A_TGCA'T
Cuts at: 0 791 1542
Size: 791 751
NspI r_CATG'y
Cuts at: 0 221 1542
Size: 221 1321
PflMI CCAn_nnn'nTGG
Cuts at: 0 312 1542
Size: 312 1230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 732 764 1542
Size: 732 32 778
Fragments arranged by size:
778 732 32
PpuMI rG'GwC_Cy
Cuts at: 0 497 1542
Size: 497 1045
PspOMI G'GGCC_C
Cuts at: 0 512 1542
Size: 512 1030
PstI C_TGCA'G
Cuts at: 0 567 1542
Size: 567 975
SanDI GG'GwC_CC
Cuts at: 0 497 1542
Size: 497 1045
SfcI C'TryA_G
Cuts at: 0 563 1254 1542
Size: 563 691 288
Fragments arranged by size:
691 563 288
StyI C'CwwG_G
Cuts at: 0 377 696 1077 1542
Size: 377 319 381 465
Fragments arranged by size:
465 381 377 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 639 1542
Size: 639 903
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 672 704 1542
Size: 672 32 838
Fragments arranged by size:
838 672 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1275 1443 1542
Size: 1275 168 99
Fragments arranged by size:
1275 168 99
XmnI GAAnn'nnTTC
Cuts at: 0 104 1542
Size: 104 1438
Enzymes that do cut:
AarI AcuI AflIII AlfI AloI AlwNI ApaI ApaLI
ApoI AseI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI Bme1580I BmgBI BpmI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BstAPI BstXI BstYI BtgI DraIII EarI EciI Eco57MI
EcoO109I FalI Hin4I HincII MmeI MslI MspA1I NcoI
NsiI NspI PflMI PpiI PpuMI PspOMI PstI SanDI
SfcI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AscI AsiSI AvaI AvrII BaeI BbeI BbvCI
BglI BglII BlpI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI
BspEI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmaI ZraI