Restriction map for
pFN21AE0561
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvrII BamHI BbrPI
BbvI BcgI BciVI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BseAI BseRI BsiHKAI BsiWI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstYI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
Eam1105I EarI Eco47III EcoO65I EcoRI
EcoRV EcoT22I FauI FseI HgaI
HpaI KpnI Ksp632I KspI MaeI
MamI MfeI MflI MluI MroI
MseI MspA1I MunI MvnI NaeI
NciI NdeI NgoMIV NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I TseI Tth111I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 465 GTCTAC GT^[AC][GT]AC
AcyI 227 GGCGCC G[GA]^CG[CT]C
AfaI 757 GTAC GT^AC
AgeI 611 ACCGGT A^CCGGT
AlwI 491 GATCC GGATC(4/5)
AlwNI 982 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 371 CCTCAGG CC^T[ACGT]AGG
Asp700I 738 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 626 TGCGCA TGC^GCA
BalI 351 TGGCCA TGG^CCA
BanII 328 GGGCTC G[GA]GC[CT]^C
BbeI 227 GGCGCC GGCGC^C
BbvCI 187 CCTCAGC CCTCAGC(-5/-2)
BclI 693 TGATCA T^GATCA
BglI 361 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 328 GGGCTC G[AGT]GC[ACT]^C
BpmI 988 CTGGAG CTGGAG(16/14)
BsaAI 932 TACGTA [CT]AC^GT[GA]
BsaHI 227 GGCGCC G[GA]^CG[CT]C
BsaI 976 GAGACC GGTCTC(1/5)
BsaWI 611 ACCGGT [AT]^CCGG[AT]
BsgI 567 GTGCAG GTGCAG(16/14)
BsiEI 198 CGGCCG CG[GA][CT]^CG
BsmBI 679 GAGACG CGTCTC(1/5)
Bsp1286I 328 GGGCTC G[AGT]GC[ACT]^C
BspHI 279 TCATGA T^CATGA
BspMI 660 ACCTGC ACCTGC(4/8)
BstAPI 642 GCATGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 318 CCACCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 371 CCTCAGG CC^T[ACGT]AGG
DsaI 339 CCATGG C^C[GA][CT]GG
EagI 198 CGGCCG C^GGCCG
EclXI 198 CGGCCG C^GGCCG
Eco52I 198 CGGCCG C^GGCCG
Eco81I 371 CCTCAGG CC^T[ACGT]AGG
EcoT14I 339 CCATGG C^C[AT][AT]GG
FbaI 693 TGATCA T^GATCA
FspI 626 TGCGCA TGC^GCA
HaeII 227 GGCGCC [GA]GCGC^[CT]
Hin1I 227 GGCGCC G[GA]^CG[CT]C
HindIII 265 AAGCTT A^AGCTT
KasI 227 GGCGCC G^GCGCC
MluNI 351 TGGCCA TGG^CCA
MscI 351 TGGCCA TGG^CCA
NarI 227 GGCGCC GG^CGCC
NcoI 339 CCATGG C^CATGG
NotI 197 GCGGCCGC GC^GGCCGC
PinAI 611 ACCGGT A^CCGGT
PpuMI 20 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 279 TCATGA T^CATGA
RsaI 757 GTAC GT^AC
SexAI 113 ACCTGGT A^CC[AT]GGT
SfoI 227 GGCGCC GGC^GCC
SnaBI 932 TACGTA TAC^GTA
StuI 368 AGGCCT AGG^CCT
StyI 339 CCATGG C^C[AT][AT]GG
TaqI 489 TCGA T^CGA
TfiI 879 GAATC G^A[AT]TC
Tsp45I 142 GTCAC ^GT[GC]AC
Tsp509I 996 AATT ^AATT
TthHB8I 489 TCGA T^CGA
XcmI 339 CCATGGATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 738 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AluI 266 AGCT AG^CT
986 AGCT
BanI 227 GGCGCC G^G[CT][GA]CC
648 GGTGCC
BbsI 180 GAAGAC GAAGAC(2/6)
273 GAAGAC
BpuAI 180 GAAGAC GAAGAC(2/6)
273 GAAGAC
BsmAI 679 GAGAC GTCTC(1/5)
976 GAGAC
BsrFI 203 GCCGGT [GA]^CCGG[CT]
611 ACCGGT
CfoI 228 GCGC GCG^C
627 GCGC
Cfr10I 203 GCCGGT [GA]^CCGG[CT]
611 ACCGGT
EaeI 198 CGGCCG [CT]^GGCC[GA]
351 TGGCCA
EcoNI 187 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
212 CCTGTGTGAGG
HapII 204 CCGG C^CGG
612 CCGG
HhaI 228 GCGC GCG^C
627 GCGC
HinP1I 228 GCGC G^CGC
627 GCGC
HincII 103 GTCAAC GT[CT]^[GA]AC
724 GTCAAC
HindII 103 GTCAAC GT[CT]^[GA]AC
724 GTCAAC
HpaII 204 CCGG C^CGG
612 CCGG
MaeII 786 ACGT A^CGT
933 ACGT
MaeIII 142 GTCAC ^GT[ACGT]AC
835 GTAAC
MslI 319 CACCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
540 CATTGTGGTG
MspI 204 CCGG C^CGG
612 CCGG
PflMI 747 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
981 CCAGGAGCTGG
SmlI 25 CTCAAG C^T[CT][GA]AG
262 CTCAAG
Van91I 747 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
981 CCAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AvaII BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
SfcI TspRI